Detailed information of oriT

oriT

The information of the oriT region

• oriTDB ID: 119631
• Name: oriT_PS15001|unnamed
• Organism: Escherichia sp. PS15001
• Sequence Completeness: -
• NCBI accession of oriT (coordinates [strand]): NZ_CP141251 (47943..48015 [-], 73 nt)
• oriT length: 73 nt
• IRs (inverted repeats): _
• Location of nic site: _
• Conserved sequence flanking the nic site: _
• Note: Predicted by oriTfinder 2.0

oriT sequence

• Length: 73 nt

>oriT_PS15001|unnamed
GTCGGGGCAAAGCCCTGACCAGGTGGGGAATGTCTGAGTGCGCGTGCGCGGTCCGACATTCCCACATCCTGTC

oriT secondary structure

Predicted by RNAfold.

Relaxase

Relaxase_VJF69_RS00300_PS15001|unnamed

• ID: 12408
• GenBank: WP_029391987
• Name: Relaxase_VJF69_RS00300_PS15001|unnamed
• UniProt ID: A0A2H5C199
• Length: 906 a.a.
• Note: Predicted by oriTfinder 2.0

Relaxase protein sequence

• Length: 906 a.a.
• Molecular weight: 105112.51 Da
• Isoelectric Point: 8.2682

>WP_029391987.1 MULTISPECIES: relaxase/mobilization nuclease domain-containing protein [Enterobacteriaceae]
MNAIIPHKRRDGRSSFEDLIAYTSVRDDVQENELTEDSEIKSDVPHRNRFRRLVDYATRLRNEKFVSLID
VMKDGSQWVNFYGVTCFHNCNSLETAAEEMQYKADKAVFSRNDTDPVFHYILSWPAHESPRPEQLFDCVR
HTLKSLELSKHQYVAAVHTDTDNLHVHVAVNRVHPETGYINWLSWSQEKLSRACRELELKHGFAPDNGCF
VHAPGNRIVRKTALVRERRNAWRRGKKQTFREYIAQMSIAGLREEPAQDWLSLHKRLASDGLYITMQEGE
LVVKDGWDRAREGVALSSFGPSWTAEKLGRKLGEYQPVPTDIFSQVGTPGRYDPEAINVDIRPEKVAETE
SLKQYACRHFAERLPEMARNGELESCLDVHRTLAKAGLWMGIQHGHLVLHDGFDKQQTPVRADSVWPLMT
LDYMQDLDGGWQPVPKDIFTQVIPGERFRGHSIGTQAVSDYEWYRMRMGTGPQGAIKRELFSDKESLWGY
ALIQCRSQIEEMIAGGDFSWQACHEMFARKGLMLQKQHHGLVIVDAFNHELTPVKASSIHPDLTLSRAEP
QAGPFEIAGADIFERVKPECRYNPELAASDEVEPGFRRDPVLRRERREARAAAREDLRARYLAWKEHWRK
PDLRYGERLREIHAACRRRKAYIRVQFRDPQVRKLHYHIAEVQRMQALIRLKESVKEERLSLIAEGKWYP
LSYRQWVEQQAAQGDRAAVSQLRGWDYRDRRSRNKDKRRTTNADRCVVLCEPGGTPLFNNVAKLEARLQK
NGSVHFRDTRTGKNVCTDYGDRVVFYHHTDRNELAEKLDLIAPVLFSRNGKLGFEPEGSYQQFNDVFAEM
VAWHNAAGITGNGHFTITRPDVDLHRQRSEQYYREYIRQQTRLSESHDDNYTLRQEKTWEPPSPGM

Protein domains

Predicted by InterproScan.

• Relaxase/Mobilisation nuclease domain (Relaxase) (62-315)

Protein structure

• AlphaFold DB: A0A2H5C199

Auxiliary protein

WP_001291058_PS15001|unnamed

• ID: 7064
• GenBank: WP_001291058
• Name: WP_001291058_PS15001|unnamed
• UniProt ID: A0A7I0L241
• Length: 110 a.a.
• PDB ID: _
• Note: Predicted by oriTfinder 2.0

Auxiliary protein sequence

• Length: 110 a.a.
• Molecular weight: 12730.52 Da
• Isoelectric Point: 9.9656

>WP_001291058.1 MULTISPECIES: plasmid mobilization protein MobA [Enterobacteriaceae]
MSEKKTRTGSENRKRIVKFTARFTEDEAEIVREKAESSGQTVSTFIRSSSLDKVVNCRTDDRMIDEIMRL
GRLQKHLFVEGKRTGDKEYAEVLVAITEYVNALRRDLMGR

Protein domains

No domain identified.

Protein structure

• AlphaFold DB: A0A7I0L241

T4CP

trbC_VJF69_RS00305_PS15001|unnamed

• ID: 14565
• GenBank: WP_029392471
• Name: trbC_VJF69_RS00305_PS15001|unnamed
• UniProt ID: _
• Length: 766 a.a.
• PDB ID: _
• Note: Predicted by oriTfinder 2.0

T4CP protein sequence

• Length: 766 a.a.
• Molecular weight: 87239.20 Da
• Isoelectric Point: 6.6676

>WP_029392471.1 MULTISPECIES: F-type conjugative transfer protein TrbC [Enterobacteriaceae]
MSQHHVNPDLIHRTAWGNPLWNALHNLNIIGLCLAGSIITALIWPLALPVCLLFTLVTSVIFTLQRWRCP
LRMPMTLSLDDPSQDRKVRRSLFSFWPTLFQYEADETSPARGIFYVGYRRINDIGRELWLSMDDLTRHII
FFSTTGGGKTETTFAWLLNPLCWGRGFTFVDGKAQNDTTRTIWYLSRRFGREDDIEVINFMNGGKSRSEI
IQSGEKSRPQSNTWNPFAFSTEAFTAETMQSMLPQNVQGGEWQSRAIAMNKALVFGTKFWCVRERKTMSL
QMLREHMTLEGMAKLYCRGLDDQWPEEAIAPLRNYLQDVPGFDMSLVRTPSAWTEEPRKQHAYLSGQFSE
TFTTFAETFGDVFAADAGDIDIRDSIHSDRILIVMIPALDTSAHTTSALGRMFITQQSMILARDLGYRLE
GTDAQTLEVKKYKGRFPYICILDEVGAYYTDRIAVEATQVRSLEFSLIMMAQDQERIEGQTSATNTATLM
QNAGTKFAGRIVSDDKTARTVKNAAGEEARARMGSLQRHDGLMGESWVDGNQITIQMESKIDVQDLIRLN
AGEFFTVFQGDVVPSASVYIPDSEKSCDSDPVVINRYIAIEAPRLERLRRLVPRTVQRRLPTPEHVSSII
GVLTEKTSRKRRKKHTEPYRIIDTFQDQLKRTQTSLDLLPQYDTDIESRANELWKKAVHTINNTTREERR
VCYITLNRPEEEHSGPEDIPSVKAILNTLLPLEILLPVAENNKSQTINNDHPQKSCGAQPTTDEKY

Protein domains

Predicted by InterproScan.

• TraM recognition site of TraD and TraG (TraG-D_C) (439-528)

Protein structure

No available structure.

T4SS

T4SS were predicted by using oriTfinder2.

Region 1: 53602..99820

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
VJF69_RS00305	51321..53621	-	2301	WP_029392471	F-type conjugative transfer protein TrbC	-
VJF69_RS00310	53602..54720	-	1119	WP_029391999	DsbC family protein	trbB
VJF69_RS00315	54717..56078	-	1362	WP_041498596	hypothetical protein	trbA
VJF69_RS00320	56389..56472	+	84	WP_271298668	DinQ-like type I toxin DqlB	-
VJF69_RS00325	56535..56711	-	177	WP_032172741	hypothetical protein	-
VJF69_RS00330	56862..57329	-	468	WP_000598962	thermonuclease family protein	-
VJF69_RS00335	57489..58112	+	624	WP_001155252	helix-turn-helix domain-containing protein	-
VJF69_RS00340	58310..58525	-	216	WP_000266543	hypothetical protein	-
VJF69_RS00345	58529..58897	-	369	WP_029391948	hypothetical protein	-
VJF69_RS00350	59196..59438	-	243	WP_029391947	hypothetical protein	-
VJF69_RS00355	59767..59919	+	153	WP_021532728	Hok/Gef family protein	-
VJF69_RS00360	59916..60077	-	162	WP_021580032	hypothetical protein	-
VJF69_RS00365	60233..60364	-	132	Protein_72	ethanolamine utilization protein EutE	-
VJF69_RS00370	60459..60668	+	210	WP_000062946	HEAT repeat domain-containing protein	-
VJF69_RS00375	60733..61386	-	654	WP_029391946	plasmid IncI1-type surface exclusion protein ExcA	-
VJF69_RS00380	61474..63639	-	2166	WP_029391945	DotA/TraY family protein	traY
VJF69_RS00385	63712..64281	-	570	WP_000650755	conjugal transfer protein TraX	-
VJF69_RS00390	64278..65483	-	1206	WP_029391944	conjugal transfer protein TraW	traW
VJF69_RS00395	65444..66061	-	618	WP_029391943	hypothetical protein	traV
VJF69_RS00400	66061..69105	-	3045	WP_029391942	conjugal transfer protein	traU
VJF69_RS00405	69281..69661	-	381	WP_000690886	hypothetical protein	-
VJF69_RS00410	69661..69969	-	309	WP_029391941	hypothetical protein	-
VJF69_RS00415	70165..70482	+	318	WP_000118520	quaternary ammonium compound efflux SMR transporter SugE	-
VJF69_RS00420	70479..71012	-	534	WP_001221666	lipocalin family protein	-
VJF69_RS00425	71106..72251	-	1146	WP_000976514	extended-spectrum class C beta-lactamase CMY-2	-
VJF69_RS00430	72575..73837	-	1263	WP_000608644	IS1380-like element ISEcp1 family transposase	-
VJF69_RS00435	74174..74800	-	627	WP_001597015	hypothetical protein	traT
VJF69_RS00440	74907..75158	-	252	WP_021532719	hypothetical protein	-
VJF69_RS00445	75215..75613	-	399	WP_029391936	DUF6750 family protein	traR
VJF69_RS00450	75660..76190	-	531	WP_000987022	conjugal transfer protein TraQ	traQ
VJF69_RS00455	76187..76900	-	714	WP_021532717	conjugal transfer protein TraP	traP
VJF69_RS00460	76897..78228	-	1332	WP_029391934	conjugal transfer protein TraO	traO
VJF69_RS00465	78232..79206	-	975	WP_029391933	DotH/IcmK family type IV secretion protein	traN
VJF69_RS00470	79217..79912	-	696	WP_029391932	DotI/IcmL family type IV secretion protein	traM
VJF69_RS00475	79924..80274	-	351	WP_021532713	hypothetical protein	traL
VJF69_RS00480	80291..84352	-	4062	WP_029391931	LPD7 domain-containing protein	-
VJF69_RS00485	84416..84706	-	291	WP_000817799	hypothetical protein	traK
VJF69_RS00490	84703..85851	-	1149	WP_000625247	plasmid transfer ATPase TraJ	virB11
VJF69_RS00495	85835..86671	-	837	WP_029391930	type IV secretory system conjugative DNA transfer family protein	traI
VJF69_RS00500	86668..87120	-	453	WP_001081199	DotD/TraH family lipoprotein	-
VJF69_RS00505	87358..87489	+	132	WP_257792844	hypothetical protein	-
VJF69_RS00510	87479..88681	-	1203	WP_029391929	conjugal transfer protein TraF	-
VJF69_RS00515	88771..89592	-	822	WP_000804089	hypothetical protein	traE
VJF69_RS00520	89843..90388	-	546	WP_029391928	hypothetical protein	-
VJF69_RS00525	90507..90788	-	282	WP_029391927	hypothetical protein	-
VJF69_RS00530	90829..91998	-	1170	WP_029391926	site-specific integrase	-
VJF69_RS00535	92365..92685	-	321	WP_251296269	shufflon protein B	-
VJF69_RS00540	93111..93464	+	354	WP_098716872	shufflon protein C	-
VJF69_RS00545	93708..94046	+	339	WP_072106734	prepilin, shufflon protein A	-
VJF69_RS00550	94051..95424	-	1374	WP_115747255	shufflon system plasmid conjugative transfer pilus tip adhesin PilV	-
VJF69_RS00555	95421..96128	-	708	WP_202110611	A24 family peptidase	-
VJF69_RS00560	96088..96564	-	477	WP_029391914	lytic transglycosylase domain-containing protein	virB1
VJF69_RS00565	96623..97159	-	537	WP_029391915	type 4 pilus major pilin	-
VJF69_RS00570	97210..98310	-	1101	WP_029391916	type II secretion system F family protein	-
VJF69_RS00575	98312..99820	-	1509	WP_021532701	GspE/PulE family protein	virB11
VJF69_RS00580	99917..100513	-	597	WP_029391917	hypothetical protein	-
VJF69_RS00585	100609..101163	-	555	WP_021532699	hypothetical protein	-
VJF69_RS00590	101212..101664	-	453	WP_029391918	type IV pilus biogenesis protein PilP	-
VJF69_RS00595	101654..102949	-	1296	WP_021532697	type 4b pilus protein PilO2	-
VJF69_RS00600	102970..104589	-	1620	WP_021532696	PilN family type IVB pilus formation outer membrane protein	-

Host bacterium

• ID: 20061
• GenBank: NZ_CP141251
• Plasmid name: PS15001|unnamed
• Incompatibility group: IncB/O/K/Z
• Plasmid size: 109821 bp
• Coordinate of oriT [Strand]: 47943..48015 [-]
• Host baterium: Escherichia sp. PS15001

Cargo genes

• Drug resistance gene: sul1, qacE, aac(3)-VIa, ant(3'')-Ia, blaCMY-2
• Virulence gene: htpB
• Metal resistance gene: -
• Degradation gene: -
• Symbiosis gene: -
• Anti-CRISPR: -