Detailed information of oriT

oriT

The information of the oriT region


oriTDB ID   119619
Name   oriT_SB18102|unnamed in_silico
Organism   Escherichia sp. SB18102
Sequence Completeness      -
NCBI accession of oriT (coordinates [strand])   NZ_CP141237 (71452..71524 [-], 73 nt)
oriT length   73 nt
IRs (inverted repeats)     _
Location of nic site      _
Conserved sequence flanking the
  nic site    		  _
Note   Predicted by oriTfinder 2.0

  oriT sequence  


Download         Length: 73 nt

>oriT_SB18102|unnamed
GTCGGGGCAAAGCCCTGACCAGGTGGGGAATGTCTGAGTGCGCGTGCGCGGTCCGACATTCCCACATCCTGTC

Visualization of oriT structure

  oriT secondary structure

Predicted by RNAfold.

Download structure file


Relaxase

ID   12396 GenBank   WP_029391987
Name   Relaxase_VJF91_RS00435_SB18102|unnamed insolico UniProt ID   A0A2H5C199
Length   906 a.a. PDB ID   
Note   Predicted by oriTfinder 2.0

  Relaxase protein sequence


Download         Length: 906 a.a.        Molecular weight: 105112.51 Da        Isoelectric Point: 8.2682

>WP_029391987.1 MULTISPECIES: relaxase/mobilization nuclease domain-containing protein [Enterobacteriaceae]
MNAIIPHKRRDGRSSFEDLIAYTSVRDDVQENELTEDSEIKSDVPHRNRFRRLVDYATRLRNEKFVSLID
VMKDGSQWVNFYGVTCFHNCNSLETAAEEMQYKADKAVFSRNDTDPVFHYILSWPAHESPRPEQLFDCVR
HTLKSLELSKHQYVAAVHTDTDNLHVHVAVNRVHPETGYINWLSWSQEKLSRACRELELKHGFAPDNGCF
VHAPGNRIVRKTALVRERRNAWRRGKKQTFREYIAQMSIAGLREEPAQDWLSLHKRLASDGLYITMQEGE
LVVKDGWDRAREGVALSSFGPSWTAEKLGRKLGEYQPVPTDIFSQVGTPGRYDPEAINVDIRPEKVAETE
SLKQYACRHFAERLPEMARNGELESCLDVHRTLAKAGLWMGIQHGHLVLHDGFDKQQTPVRADSVWPLMT
LDYMQDLDGGWQPVPKDIFTQVIPGERFRGHSIGTQAVSDYEWYRMRMGTGPQGAIKRELFSDKESLWGY
ALIQCRSQIEEMIAGGDFSWQACHEMFARKGLMLQKQHHGLVIVDAFNHELTPVKASSIHPDLTLSRAEP
QAGPFEIAGADIFERVKPECRYNPELAASDEVEPGFRRDPVLRRERREARAAAREDLRARYLAWKEHWRK
PDLRYGERLREIHAACRRRKAYIRVQFRDPQVRKLHYHIAEVQRMQALIRLKESVKEERLSLIAEGKWYP
LSYRQWVEQQAAQGDRAAVSQLRGWDYRDRRSRNKDKRRTTNADRCVVLCEPGGTPLFNNVAKLEARLQK
NGSVHFRDTRTGKNVCTDYGDRVVFYHHTDRNELAEKLDLIAPVLFSRNGKLGFEPEGSYQQFNDVFAEM
VAWHNAAGITGNGHFTITRPDVDLHRQRSEQYYREYIRQQTRLSESHDDNYTLRQEKTWEPPSPGM

  Protein domains


Predicted by InterproScan.

Relaxase/Mobilisation nuclease domain (Relaxase)

(62-315)


  Protein structure


Source ID Structure
AlphaFold DB A0A2H5C199


Auxiliary protein

ID   7052 GenBank   WP_001291058
Name   WP_001291058_SB18102|unnamed insolico UniProt ID   A0A7I0L241
Length   110 a.a. PDB ID   _
Note   Predicted by oriTfinder 2.0

  Auxiliary protein sequence


Download         Length: 110 a.a.        Molecular weight: 12730.52 Da        Isoelectric Point: 9.9656

>WP_001291058.1 MULTISPECIES: plasmid mobilization protein MobA [Enterobacteriaceae]
MSEKKTRTGSENRKRIVKFTARFTEDEAEIVREKAESSGQTVSTFIRSSSLDKVVNCRTDDRMIDEIMRL
GRLQKHLFVEGKRTGDKEYAEVLVAITEYVNALRRDLMGR

  Protein domains



No domain identified.



  Protein structure


Source ID Structure
AlphaFold DB A0A7I0L241


T4CP

ID   14554 GenBank   WP_029392471
Name   trbC_VJF91_RS00440_SB18102|unnamed insolico UniProt ID   _
Length   766 a.a. PDB ID   _
Note   Predicted by oriTfinder 2.0

  T4CP protein sequence


Download         Length: 766 a.a.        Molecular weight: 87239.20 Da        Isoelectric Point: 6.6676

>WP_029392471.1 MULTISPECIES: F-type conjugative transfer protein TrbC [Enterobacteriaceae]
MSQHHVNPDLIHRTAWGNPLWNALHNLNIIGLCLAGSIITALIWPLALPVCLLFTLVTSVIFTLQRWRCP
LRMPMTLSLDDPSQDRKVRRSLFSFWPTLFQYEADETSPARGIFYVGYRRINDIGRELWLSMDDLTRHII
FFSTTGGGKTETTFAWLLNPLCWGRGFTFVDGKAQNDTTRTIWYLSRRFGREDDIEVINFMNGGKSRSEI
IQSGEKSRPQSNTWNPFAFSTEAFTAETMQSMLPQNVQGGEWQSRAIAMNKALVFGTKFWCVRERKTMSL
QMLREHMTLEGMAKLYCRGLDDQWPEEAIAPLRNYLQDVPGFDMSLVRTPSAWTEEPRKQHAYLSGQFSE
TFTTFAETFGDVFAADAGDIDIRDSIHSDRILIVMIPALDTSAHTTSALGRMFITQQSMILARDLGYRLE
GTDAQTLEVKKYKGRFPYICILDEVGAYYTDRIAVEATQVRSLEFSLIMMAQDQERIEGQTSATNTATLM
QNAGTKFAGRIVSDDKTARTVKNAAGEEARARMGSLQRHDGLMGESWVDGNQITIQMESKIDVQDLIRLN
AGEFFTVFQGDVVPSASVYIPDSEKSCDSDPVVINRYIAIEAPRLERLRRLVPRTVQRRLPTPEHVSSII
GVLTEKTSRKRRKKHTEPYRIIDTFQDQLKRTQTSLDLLPQYDTDIESRANELWKKAVHTINNTTREERR
VCYITLNRPEEEHSGPEDIPSVKAILNTLLPLEILLPVAENNKSQTINNDHPQKSCGAQPTTDEKY

  Protein domains


Predicted by InterproScan.

TraM recognition site of TraD and TraG (TraG-D_C)

(439-528)

  Protein structure



No available structure.




T4SS

T4SS were predicted by using oriTfinder2.

Region 1: 77111..123311

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
VJF91_RS00440 74830..77130 - 2301 WP_029392471 F-type conjugative transfer protein TrbC -
VJF91_RS00445 77111..78229 - 1119 WP_029391999 DsbC family protein trbB
VJF91_RS00450 78226..79569 - 1344 WP_172686572 conjugal transfer protein TrbA trbA
VJF91_RS00455 79880..79963 + 84 WP_271298668 DinQ-like type I toxin DqlB -
VJF91_RS00460 80026..80202 - 177 WP_032172741 hypothetical protein -
VJF91_RS00465 80353..80820 - 468 WP_000598962 thermonuclease family protein -
VJF91_RS00470 80980..81603 + 624 WP_001155252 helix-turn-helix domain-containing protein -
VJF91_RS00475 81801..82016 - 216 WP_000266543 hypothetical protein -
VJF91_RS00480 82020..82388 - 369 WP_029391948 hypothetical protein -
VJF91_RS00485 82687..82929 - 243 WP_029391947 hypothetical protein -
VJF91_RS00490 83258..83410 + 153 WP_021532728 Hok/Gef family protein -
VJF91_RS00495 83724..83855 - 132 Protein_98 ethanolamine utilization protein EutE -
VJF91_RS00500 83950..84159 + 210 WP_000062946 HEAT repeat domain-containing protein -
VJF91_RS00505 84224..84877 - 654 WP_029391946 plasmid IncI1-type surface exclusion protein ExcA -
VJF91_RS00510 84965..87130 - 2166 WP_029391945 DotA/TraY family protein traY
VJF91_RS00515 87203..87772 - 570 WP_000650755 conjugal transfer protein TraX -
VJF91_RS00520 87769..88974 - 1206 WP_029391944 conjugal transfer protein TraW traW
VJF91_RS00525 88935..89552 - 618 WP_029391943 hypothetical protein traV
VJF91_RS00530 89552..92596 - 3045 WP_029391942 conjugal transfer protein traU
VJF91_RS00535 92772..93152 - 381 WP_000690886 hypothetical protein -
VJF91_RS00540 93152..93460 - 309 WP_029391941 hypothetical protein -
VJF91_RS00545 93656..93973 + 318 WP_000118520 quaternary ammonium compound efflux SMR transporter SugE -
VJF91_RS00550 93970..94503 - 534 WP_001221666 lipocalin family protein -
VJF91_RS00555 94597..95742 - 1146 WP_000976514 extended-spectrum class C beta-lactamase CMY-2 -
VJF91_RS00560 96066..97328 - 1263 WP_000608644 IS1380-like element ISEcp1 family transposase -
VJF91_RS00565 97665..98483 - 819 WP_072132149 conjugal transfer protein TraT traT
VJF91_RS00570 98398..98649 - 252 WP_021532719 hypothetical protein -
VJF91_RS00575 98706..99104 - 399 WP_029391936 DUF6750 family protein traR
VJF91_RS00580 99151..99681 - 531 WP_000987022 conjugal transfer protein TraQ traQ
VJF91_RS00585 99678..100391 - 714 WP_021532717 conjugal transfer protein TraP traP
VJF91_RS00590 100388..101719 - 1332 WP_323873455 conjugal transfer protein TraO traO
VJF91_RS00595 101723..102697 - 975 WP_029391933 DotH/IcmK family type IV secretion protein traN
VJF91_RS00600 102708..103403 - 696 WP_029391932 DotI/IcmL family type IV secretion protein traM
VJF91_RS00605 103415..103765 - 351 WP_021532713 hypothetical protein traL
VJF91_RS00610 103782..107843 - 4062 WP_029391931 LPD7 domain-containing protein -
VJF91_RS00615 107907..108197 - 291 WP_000817799 hypothetical protein traK
VJF91_RS00620 108194..109342 - 1149 WP_000625247 plasmid transfer ATPase TraJ virB11
VJF91_RS00625 109326..110162 - 837 WP_029391930 type IV secretory system conjugative DNA transfer family protein traI
VJF91_RS00630 110159..110611 - 453 WP_001081199 DotD/TraH family lipoprotein -
VJF91_RS00635 110849..110980 + 132 WP_257792844 hypothetical protein -
VJF91_RS00640 110970..112172 - 1203 WP_029391929 conjugal transfer protein TraF -
VJF91_RS00645 112262..113083 - 822 WP_000804089 hypothetical protein traE
VJF91_RS00650 113334..113879 - 546 WP_029391928 hypothetical protein -
VJF91_RS00655 113998..114279 - 282 WP_029391927 hypothetical protein -
VJF91_RS00660 114320..115489 - 1170 WP_029391926 site-specific integrase -
VJF91_RS00665 116058..116378 + 321 WP_236922167 shufflon protein B -
VJF91_RS00670 116557..116955 + 399 WP_309388060 shufflon protein C -
VJF91_RS00675 117199..117537 + 339 WP_072106734 prepilin, shufflon protein A -
VJF91_RS00680 117542..118915 - 1374 WP_115747255 shufflon system plasmid conjugative transfer pilus tip adhesin PilV -
VJF91_RS00685 118912..119619 - 708 WP_202110611 A24 family peptidase -
VJF91_RS00690 119579..120055 - 477 WP_029391914 lytic transglycosylase domain-containing protein virB1
VJF91_RS00695 120114..120650 - 537 WP_029391915 type 4 pilus major pilin -
VJF91_RS00700 120701..121801 - 1101 WP_029391916 type II secretion system F family protein -
VJF91_RS00705 121803..123311 - 1509 WP_021532701 GspE/PulE family protein virB11
VJF91_RS00710 123408..124004 - 597 WP_029391917 hypothetical protein -
VJF91_RS00715 124100..124654 - 555 WP_021532699 hypothetical protein -
VJF91_RS00720 124703..125155 - 453 WP_029391918 type IV pilus biogenesis protein PilP -
VJF91_RS00725 125145..126065 - 921 WP_323873456 type 4b pilus protein PilO2 -
VJF91_RS00730 126158..126346 + 189 WP_323873457 hypothetical protein -
VJF91_RS00735 126460..128079 - 1620 WP_021532696 PilN family type IVB pilus formation outer membrane protein -

Host bacterium

ID   20049 GenBank   NZ_CP141237
Plasmid name   SB18102|unnamed Incompatibility group   IncB/O/K/Z
Plasmid size   133311 bp Coordinate of oriT [Strand]   71452..71524 [-]
Host baterium   Escherichia sp. SB18102

Cargo genes

Drug resistance gene   tet(A), dfrA1, blaTEM-1B, sul2, blaCMY-2
Virulence gene   -
Metal resistance gene   -
Degradation gene   -
Symbiosis gene   -
Anti-CRISPR   -