Detailed information of oriT

oriT

The information of the oriT region

• oriTDB ID: 119616
• Name: oriT_SB17032|unnamed
• Organism: Escherichia sp. SB17032
• Sequence Completeness: -
• NCBI accession of oriT (coordinates [strand]): NZ_CP141231 (24078..24150 [-], 73 nt)
• oriT length: 73 nt
• IRs (inverted repeats): _
• Location of nic site: _
• Conserved sequence flanking the nic site: _
• Note: Predicted by oriTfinder 2.0

oriT sequence

• Length: 73 nt

>oriT_SB17032|unnamed
GTCGGGGCAAAGCCCTGACCAGGTGGGGAATGTCTGAGTGCGCGTGCGCGGTCCGACATTCCCACATCCTGTC

oriT secondary structure

Predicted by RNAfold.

Relaxase

Relaxase_VJF77_RS00195_SB17032|unnamed

• ID: 12393
• GenBank: WP_029391987
• Name: Relaxase_VJF77_RS00195_SB17032|unnamed
• UniProt ID: A0A2H5C199
• Length: 906 a.a.
• Note: Predicted by oriTfinder 2.0

Relaxase protein sequence

• Length: 906 a.a.
• Molecular weight: 105112.51 Da
• Isoelectric Point: 8.2682

>WP_029391987.1 MULTISPECIES: relaxase/mobilization nuclease domain-containing protein [Enterobacteriaceae]
MNAIIPHKRRDGRSSFEDLIAYTSVRDDVQENELTEDSEIKSDVPHRNRFRRLVDYATRLRNEKFVSLID
VMKDGSQWVNFYGVTCFHNCNSLETAAEEMQYKADKAVFSRNDTDPVFHYILSWPAHESPRPEQLFDCVR
HTLKSLELSKHQYVAAVHTDTDNLHVHVAVNRVHPETGYINWLSWSQEKLSRACRELELKHGFAPDNGCF
VHAPGNRIVRKTALVRERRNAWRRGKKQTFREYIAQMSIAGLREEPAQDWLSLHKRLASDGLYITMQEGE
LVVKDGWDRAREGVALSSFGPSWTAEKLGRKLGEYQPVPTDIFSQVGTPGRYDPEAINVDIRPEKVAETE
SLKQYACRHFAERLPEMARNGELESCLDVHRTLAKAGLWMGIQHGHLVLHDGFDKQQTPVRADSVWPLMT
LDYMQDLDGGWQPVPKDIFTQVIPGERFRGHSIGTQAVSDYEWYRMRMGTGPQGAIKRELFSDKESLWGY
ALIQCRSQIEEMIAGGDFSWQACHEMFARKGLMLQKQHHGLVIVDAFNHELTPVKASSIHPDLTLSRAEP
QAGPFEIAGADIFERVKPECRYNPELAASDEVEPGFRRDPVLRRERREARAAAREDLRARYLAWKEHWRK
PDLRYGERLREIHAACRRRKAYIRVQFRDPQVRKLHYHIAEVQRMQALIRLKESVKEERLSLIAEGKWYP
LSYRQWVEQQAAQGDRAAVSQLRGWDYRDRRSRNKDKRRTTNADRCVVLCEPGGTPLFNNVAKLEARLQK
NGSVHFRDTRTGKNVCTDYGDRVVFYHHTDRNELAEKLDLIAPVLFSRNGKLGFEPEGSYQQFNDVFAEM
VAWHNAAGITGNGHFTITRPDVDLHRQRSEQYYREYIRQQTRLSESHDDNYTLRQEKTWEPPSPGM

Protein domains

Predicted by InterproScan.

• Relaxase/Mobilisation nuclease domain (Relaxase) (62-315)

Protein structure

• AlphaFold DB: A0A2H5C199

Auxiliary protein

WP_001291058_SB17032|unnamed

• ID: 7049
• GenBank: WP_001291058
• Name: WP_001291058_SB17032|unnamed
• UniProt ID: A0A7I0L241
• Length: 110 a.a.
• PDB ID: _
• Note: Predicted by oriTfinder 2.0

Auxiliary protein sequence

• Length: 110 a.a.
• Molecular weight: 12730.52 Da
• Isoelectric Point: 9.9656

>WP_001291058.1 MULTISPECIES: plasmid mobilization protein MobA [Enterobacteriaceae]
MSEKKTRTGSENRKRIVKFTARFTEDEAEIVREKAESSGQTVSTFIRSSSLDKVVNCRTDDRMIDEIMRL
GRLQKHLFVEGKRTGDKEYAEVLVAITEYVNALRRDLMGR

Protein domains

No domain identified.

Protein structure

• AlphaFold DB: A0A7I0L241

T4CP

trbC_VJF77_RS00200_SB17032|unnamed

• ID: 14552
• GenBank: WP_029392471
• Name: trbC_VJF77_RS00200_SB17032|unnamed
• UniProt ID: _
• Length: 766 a.a.
• PDB ID: _
• Note: Predicted by oriTfinder 2.0

T4CP protein sequence

• Length: 766 a.a.
• Molecular weight: 87239.20 Da
• Isoelectric Point: 6.6676

>WP_029392471.1 MULTISPECIES: F-type conjugative transfer protein TrbC [Enterobacteriaceae]
MSQHHVNPDLIHRTAWGNPLWNALHNLNIIGLCLAGSIITALIWPLALPVCLLFTLVTSVIFTLQRWRCP
LRMPMTLSLDDPSQDRKVRRSLFSFWPTLFQYEADETSPARGIFYVGYRRINDIGRELWLSMDDLTRHII
FFSTTGGGKTETTFAWLLNPLCWGRGFTFVDGKAQNDTTRTIWYLSRRFGREDDIEVINFMNGGKSRSEI
IQSGEKSRPQSNTWNPFAFSTEAFTAETMQSMLPQNVQGGEWQSRAIAMNKALVFGTKFWCVRERKTMSL
QMLREHMTLEGMAKLYCRGLDDQWPEEAIAPLRNYLQDVPGFDMSLVRTPSAWTEEPRKQHAYLSGQFSE
TFTTFAETFGDVFAADAGDIDIRDSIHSDRILIVMIPALDTSAHTTSALGRMFITQQSMILARDLGYRLE
GTDAQTLEVKKYKGRFPYICILDEVGAYYTDRIAVEATQVRSLEFSLIMMAQDQERIEGQTSATNTATLM
QNAGTKFAGRIVSDDKTARTVKNAAGEEARARMGSLQRHDGLMGESWVDGNQITIQMESKIDVQDLIRLN
AGEFFTVFQGDVVPSASVYIPDSEKSCDSDPVVINRYIAIEAPRLERLRRLVPRTVQRRLPTPEHVSSII
GVLTEKTSRKRRKKHTEPYRIIDTFQDQLKRTQTSLDLLPQYDTDIESRANELWKKAVHTINNTTREERR
VCYITLNRPEEEHSGPEDIPSVKAILNTLLPLEILLPVAENNKSQTINNDHPQKSCGAQPTTDEKY

Protein domains

Predicted by InterproScan.

• TraM recognition site of TraD and TraG (TraG-D_C) (439-528)

Protein structure

No available structure.

T4SS

T4SS were predicted by using oriTfinder2.

Region 1: 29737..75946

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
VJF77_RS00200	27456..29756	-	2301	WP_029392471	F-type conjugative transfer protein TrbC	-
VJF77_RS00205	29737..30855	-	1119	WP_029391999	DsbC family protein	trbB
VJF77_RS00210	30852..32204	-	1353	WP_065749868	conjugal transfer protein TrbA	trbA
VJF77_RS00215	32515..32598	+	84	WP_271298668	DinQ-like type I toxin DqlB	-
VJF77_RS00220	32661..32837	-	177	WP_032172741	hypothetical protein	-
VJF77_RS00225	32988..33455	-	468	WP_000598962	thermonuclease family protein	-
VJF77_RS00230	33615..34238	+	624	WP_001155252	helix-turn-helix domain-containing protein	-
VJF77_RS00235	34436..34651	-	216	WP_000266543	hypothetical protein	-
VJF77_RS00240	34655..35023	-	369	WP_029391948	hypothetical protein	-
VJF77_RS00245	35322..35564	-	243	WP_029391947	hypothetical protein	-
VJF77_RS00250	35563..35748	+	186	WP_024194018	hypothetical protein	-
VJF77_RS00255	35893..36045	+	153	WP_021532728	Hok/Gef family protein	-
VJF77_RS00260	36042..36203	-	162	WP_021580032	hypothetical protein	-
VJF77_RS00265	36359..36490	-	132	Protein_52	ethanolamine utilization protein EutE	-
VJF77_RS00270	36585..36794	+	210	WP_000062946	HEAT repeat domain-containing protein	-
VJF77_RS00275	36859..37512	-	654	WP_029391946	plasmid IncI1-type surface exclusion protein ExcA	-
VJF77_RS00280	37600..39765	-	2166	WP_029391945	DotA/TraY family protein	traY
VJF77_RS00285	39838..40407	-	570	WP_000650755	conjugal transfer protein TraX	-
VJF77_RS00290	40404..41609	-	1206	WP_029391944	conjugal transfer protein TraW	traW
VJF77_RS00295	41570..42187	-	618	WP_029391943	hypothetical protein	traV
VJF77_RS00300	42187..45231	-	3045	WP_029391942	conjugal transfer protein	traU
VJF77_RS00305	45407..45787	-	381	WP_000690886	hypothetical protein	-
VJF77_RS00310	45787..46095	-	309	WP_029391941	hypothetical protein	-
VJF77_RS00315	46291..46608	+	318	WP_000118520	quaternary ammonium compound efflux SMR transporter SugE	-
VJF77_RS00320	46605..47138	-	534	WP_001221666	lipocalin family protein	-
VJF77_RS00325	47232..48377	-	1146	WP_000976514	extended-spectrum class C beta-lactamase CMY-2	-
VJF77_RS00330	48701..49963	-	1263	WP_000608644	IS1380-like element ISEcp1 family transposase	-
VJF77_RS00335	50300..50926	-	627	WP_001597015	hypothetical protein	traT
VJF77_RS00340	51033..51284	-	252	WP_021532719	hypothetical protein	-
VJF77_RS00345	51341..51739	-	399	WP_029391936	DUF6750 family protein	traR
VJF77_RS00350	51786..52316	-	531	WP_000987022	conjugal transfer protein TraQ	traQ
VJF77_RS00355	52313..53026	-	714	WP_021532717	conjugal transfer protein TraP	traP
VJF77_RS00360	53023..54354	-	1332	WP_029391934	conjugal transfer protein TraO	traO
VJF77_RS00365	54358..55332	-	975	WP_029391933	DotH/IcmK family type IV secretion protein	traN
VJF77_RS00370	55343..56038	-	696	WP_029391932	DotI/IcmL family type IV secretion protein	traM
VJF77_RS00375	56050..56400	-	351	WP_021532713	hypothetical protein	traL
VJF77_RS00380	56417..60478	-	4062	WP_029391931	LPD7 domain-containing protein	-
VJF77_RS00385	60542..60832	-	291	WP_000817799	hypothetical protein	traK
VJF77_RS00390	60829..61977	-	1149	WP_000625247	plasmid transfer ATPase TraJ	virB11
VJF77_RS00395	61961..62797	-	837	WP_029391930	type IV secretory system conjugative DNA transfer family protein	traI
VJF77_RS00400	62794..63246	-	453	WP_001081199	DotD/TraH family lipoprotein	-
VJF77_RS00405	63605..64807	-	1203	WP_029391929	conjugal transfer protein TraF	-
VJF77_RS00410	64897..65718	-	822	WP_000804089	hypothetical protein	traE
VJF77_RS00415	65969..66514	-	546	WP_029391928	hypothetical protein	-
VJF77_RS00420	66633..66926	-	294	WP_236918885	hypothetical protein	-
VJF77_RS00425	66955..68124	-	1170	WP_029391926	site-specific integrase	-
VJF77_RS00430	68179..68451	+	273	WP_048227902	hypothetical protein	-
VJF77_RS00435	68765..69154	+	390	Protein_86	phage tail protein	-
VJF77_RS00440	69159..69497	-	339	WP_072106734	prepilin, shufflon protein A	-
VJF77_RS00445	70306..71550	-	1245	WP_119799357	shufflon system plasmid conjugative transfer pilus tip adhesin PilV	-
VJF77_RS00450	71547..72254	-	708	WP_202110611	A24 family peptidase	-
VJF77_RS00455	72214..72690	-	477	WP_029391914	lytic transglycosylase domain-containing protein	virB1
VJF77_RS00460	72749..73285	-	537	WP_029391915	type 4 pilus major pilin	-
VJF77_RS00465	73336..74436	-	1101	WP_029391916	type II secretion system F family protein	-
VJF77_RS00470	74438..75946	-	1509	WP_269548998	GspE/PulE family protein	virB11
VJF77_RS00475	76043..76639	-	597	WP_029391917	hypothetical protein	-
VJF77_RS00480	76735..77289	-	555	WP_021532699	hypothetical protein	-
VJF77_RS00485	77338..77790	-	453	WP_029391918	type IV pilus biogenesis protein PilP	-
VJF77_RS00490	77780..79075	-	1296	WP_021532697	type 4b pilus protein PilO2	-
VJF77_RS00495	79096..80715	-	1620	WP_021532696	PilN family type IVB pilus formation outer membrane protein	-

Host bacterium

• ID: 20046
• GenBank: NZ_CP141231
• Plasmid name: SB17032|unnamed
• Incompatibility group: IncB/O/K/Z
• Plasmid size: 85947 bp
• Coordinate of oriT [Strand]: 24078..24150 [-]
• Host baterium: Escherichia sp. SB17032

Cargo genes

• Drug resistance gene: blaCMY-2
• Virulence gene: -
• Metal resistance gene: -
• Degradation gene: -
• Symbiosis gene: -
• Anti-CRISPR: -