Detailed information of oriT

oriT

The information of the oriT region

• oriTDB ID: 119614
• Name: oriT_HBI03|unnamed
• Organism: Escherichia sp. HBI03
• Sequence Completeness: -
• NCBI accession of oriT (coordinates [strand]): NZ_CP141229 (24085..24157 [-], 73 nt)
• oriT length: 73 nt
• IRs (inverted repeats): _
• Location of nic site: _
• Conserved sequence flanking the nic site: _
• Note: Predicted by oriTfinder 2.0

oriT sequence

• Length: 73 nt

>oriT_HBI03|unnamed
GTCGGGGCAAAGCCCTGACCAGGTGGGGAATGTCTGAGTGCGCGTGCGCGGTCCGACATTCCCACATCCTGTC

oriT secondary structure

Predicted by RNAfold.

Relaxase

Relaxase_VJF81_RS00195_HBI03|unnamed

• ID: 12391
• GenBank: WP_029391987
• Name: Relaxase_VJF81_RS00195_HBI03|unnamed
• UniProt ID: A0A2H5C199
• Length: 906 a.a.
• Note: Predicted by oriTfinder 2.0

Relaxase protein sequence

• Length: 906 a.a.
• Molecular weight: 105112.51 Da
• Isoelectric Point: 8.2682

>WP_029391987.1 MULTISPECIES: relaxase/mobilization nuclease domain-containing protein [Enterobacteriaceae]
MNAIIPHKRRDGRSSFEDLIAYTSVRDDVQENELTEDSEIKSDVPHRNRFRRLVDYATRLRNEKFVSLID
VMKDGSQWVNFYGVTCFHNCNSLETAAEEMQYKADKAVFSRNDTDPVFHYILSWPAHESPRPEQLFDCVR
HTLKSLELSKHQYVAAVHTDTDNLHVHVAVNRVHPETGYINWLSWSQEKLSRACRELELKHGFAPDNGCF
VHAPGNRIVRKTALVRERRNAWRRGKKQTFREYIAQMSIAGLREEPAQDWLSLHKRLASDGLYITMQEGE
LVVKDGWDRAREGVALSSFGPSWTAEKLGRKLGEYQPVPTDIFSQVGTPGRYDPEAINVDIRPEKVAETE
SLKQYACRHFAERLPEMARNGELESCLDVHRTLAKAGLWMGIQHGHLVLHDGFDKQQTPVRADSVWPLMT
LDYMQDLDGGWQPVPKDIFTQVIPGERFRGHSIGTQAVSDYEWYRMRMGTGPQGAIKRELFSDKESLWGY
ALIQCRSQIEEMIAGGDFSWQACHEMFARKGLMLQKQHHGLVIVDAFNHELTPVKASSIHPDLTLSRAEP
QAGPFEIAGADIFERVKPECRYNPELAASDEVEPGFRRDPVLRRERREARAAAREDLRARYLAWKEHWRK
PDLRYGERLREIHAACRRRKAYIRVQFRDPQVRKLHYHIAEVQRMQALIRLKESVKEERLSLIAEGKWYP
LSYRQWVEQQAAQGDRAAVSQLRGWDYRDRRSRNKDKRRTTNADRCVVLCEPGGTPLFNNVAKLEARLQK
NGSVHFRDTRTGKNVCTDYGDRVVFYHHTDRNELAEKLDLIAPVLFSRNGKLGFEPEGSYQQFNDVFAEM
VAWHNAAGITGNGHFTITRPDVDLHRQRSEQYYREYIRQQTRLSESHDDNYTLRQEKTWEPPSPGM

Protein domains

Predicted by InterproScan.

• Relaxase/Mobilisation nuclease domain (Relaxase) (62-315)

Protein structure

• AlphaFold DB: A0A2H5C199

Auxiliary protein

WP_001291058_HBI03|unnamed

• ID: 7047
• GenBank: WP_001291058
• Name: WP_001291058_HBI03|unnamed
• UniProt ID: A0A7I0L241
• Length: 110 a.a.
• PDB ID: _
• Note: Predicted by oriTfinder 2.0

Auxiliary protein sequence

• Length: 110 a.a.
• Molecular weight: 12730.52 Da
• Isoelectric Point: 9.9656

>WP_001291058.1 MULTISPECIES: plasmid mobilization protein MobA [Enterobacteriaceae]
MSEKKTRTGSENRKRIVKFTARFTEDEAEIVREKAESSGQTVSTFIRSSSLDKVVNCRTDDRMIDEIMRL
GRLQKHLFVEGKRTGDKEYAEVLVAITEYVNALRRDLMGR

Protein domains

No domain identified.

Protein structure

• AlphaFold DB: A0A7I0L241

T4CP

trbC_VJF81_RS00200_HBI03|unnamed

• ID: 14550
• GenBank: WP_029392471
• Name: trbC_VJF81_RS00200_HBI03|unnamed
• UniProt ID: _
• Length: 766 a.a.
• PDB ID: _
• Note: Predicted by oriTfinder 2.0

T4CP protein sequence

• Length: 766 a.a.
• Molecular weight: 87239.20 Da
• Isoelectric Point: 6.6676

>WP_029392471.1 MULTISPECIES: F-type conjugative transfer protein TrbC [Enterobacteriaceae]
MSQHHVNPDLIHRTAWGNPLWNALHNLNIIGLCLAGSIITALIWPLALPVCLLFTLVTSVIFTLQRWRCP
LRMPMTLSLDDPSQDRKVRRSLFSFWPTLFQYEADETSPARGIFYVGYRRINDIGRELWLSMDDLTRHII
FFSTTGGGKTETTFAWLLNPLCWGRGFTFVDGKAQNDTTRTIWYLSRRFGREDDIEVINFMNGGKSRSEI
IQSGEKSRPQSNTWNPFAFSTEAFTAETMQSMLPQNVQGGEWQSRAIAMNKALVFGTKFWCVRERKTMSL
QMLREHMTLEGMAKLYCRGLDDQWPEEAIAPLRNYLQDVPGFDMSLVRTPSAWTEEPRKQHAYLSGQFSE
TFTTFAETFGDVFAADAGDIDIRDSIHSDRILIVMIPALDTSAHTTSALGRMFITQQSMILARDLGYRLE
GTDAQTLEVKKYKGRFPYICILDEVGAYYTDRIAVEATQVRSLEFSLIMMAQDQERIEGQTSATNTATLM
QNAGTKFAGRIVSDDKTARTVKNAAGEEARARMGSLQRHDGLMGESWVDGNQITIQMESKIDVQDLIRLN
AGEFFTVFQGDVVPSASVYIPDSEKSCDSDPVVINRYIAIEAPRLERLRRLVPRTVQRRLPTPEHVSSII
GVLTEKTSRKRRKKHTEPYRIIDTFQDQLKRTQTSLDLLPQYDTDIESRANELWKKAVHTINNTTREERR
VCYITLNRPEEEHSGPEDIPSVKAILNTLLPLEILLPVAENNKSQTINNDHPQKSCGAQPTTDEKY

Protein domains

Predicted by InterproScan.

• TraM recognition site of TraD and TraG (TraG-D_C) (439-528)

Protein structure

No available structure.

T4SS

T4SS were predicted by using oriTfinder2.

Region 1: 29744..75953

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
VJF81_RS00200	27463..29763	-	2301	WP_029392471	F-type conjugative transfer protein TrbC	-
VJF81_RS00205	29744..30862	-	1119	WP_029391999	DsbC family protein	trbB
VJF81_RS00210	30859..32211	-	1353	WP_065749868	conjugal transfer protein TrbA	trbA
VJF81_RS00215	32522..32605	+	84	WP_271298668	DinQ-like type I toxin DqlB	-
VJF81_RS00220	32668..32844	-	177	WP_032172741	hypothetical protein	-
VJF81_RS00225	32995..33462	-	468	WP_000598962	thermonuclease family protein	-
VJF81_RS00230	33622..34245	+	624	WP_001155252	helix-turn-helix domain-containing protein	-
VJF81_RS00235	34443..34658	-	216	WP_000266543	hypothetical protein	-
VJF81_RS00240	34662..35030	-	369	WP_029391948	hypothetical protein	-
VJF81_RS00245	35329..35571	-	243	WP_029391947	hypothetical protein	-
VJF81_RS00250	35570..35755	+	186	WP_024194018	hypothetical protein	-
VJF81_RS00255	35900..36052	+	153	WP_021532728	Hok/Gef family protein	-
VJF81_RS00260	36049..36210	-	162	WP_021580032	hypothetical protein	-
VJF81_RS00265	36366..36497	-	132	Protein_52	ethanolamine utilization protein EutE	-
VJF81_RS00270	36592..36801	+	210	WP_000062946	HEAT repeat domain-containing protein	-
VJF81_RS00275	36866..37519	-	654	WP_029391946	plasmid IncI1-type surface exclusion protein ExcA	-
VJF81_RS00280	37607..39772	-	2166	WP_029391945	DotA/TraY family protein	traY
VJF81_RS00285	39845..40414	-	570	WP_000650755	conjugal transfer protein TraX	-
VJF81_RS00290	40411..41616	-	1206	WP_029391944	conjugal transfer protein TraW	traW
VJF81_RS00295	41577..42194	-	618	WP_029391943	hypothetical protein	traV
VJF81_RS00300	42194..45238	-	3045	WP_029391942	conjugal transfer protein	traU
VJF81_RS00305	45414..45794	-	381	WP_000690886	hypothetical protein	-
VJF81_RS00310	45794..46102	-	309	WP_029391941	hypothetical protein	-
VJF81_RS00315	46298..46615	+	318	WP_000118520	quaternary ammonium compound efflux SMR transporter SugE	-
VJF81_RS00320	46612..47145	-	534	WP_001221666	lipocalin family protein	-
VJF81_RS00325	47239..48384	-	1146	WP_000976514	extended-spectrum class C beta-lactamase CMY-2	-
VJF81_RS00330	48708..49970	-	1263	WP_000608644	IS1380-like element ISEcp1 family transposase	-
VJF81_RS00335	50307..50933	-	627	WP_001597015	hypothetical protein	traT
VJF81_RS00340	51040..51291	-	252	WP_021532719	hypothetical protein	-
VJF81_RS00345	51348..51746	-	399	WP_029391936	DUF6750 family protein	traR
VJF81_RS00350	51793..52323	-	531	WP_000987022	conjugal transfer protein TraQ	traQ
VJF81_RS00355	52320..53033	-	714	WP_021532717	conjugal transfer protein TraP	traP
VJF81_RS00360	53030..54361	-	1332	WP_029391934	conjugal transfer protein TraO	traO
VJF81_RS00365	54365..55339	-	975	WP_029391933	DotH/IcmK family type IV secretion protein	traN
VJF81_RS00370	55350..56045	-	696	WP_029391932	DotI/IcmL family type IV secretion protein	traM
VJF81_RS00375	56057..56407	-	351	WP_021532713	hypothetical protein	traL
VJF81_RS00380	56424..60485	-	4062	WP_029391931	LPD7 domain-containing protein	-
VJF81_RS00385	60549..60839	-	291	WP_000817799	hypothetical protein	traK
VJF81_RS00390	60836..61984	-	1149	WP_000625247	plasmid transfer ATPase TraJ	virB11
VJF81_RS00395	61968..62804	-	837	WP_029391930	type IV secretory system conjugative DNA transfer family protein	traI
VJF81_RS00400	62801..63253	-	453	WP_001081199	DotD/TraH family lipoprotein	-
VJF81_RS00405	63612..64814	-	1203	WP_029391929	conjugal transfer protein TraF	-
VJF81_RS00410	64904..65725	-	822	WP_000804089	hypothetical protein	traE
VJF81_RS00415	65976..66521	-	546	WP_029391928	hypothetical protein	-
VJF81_RS00420	66640..66933	-	294	WP_236918885	hypothetical protein	-
VJF81_RS00425	66962..68131	-	1170	WP_029391926	site-specific integrase	-
VJF81_RS00430	68186..68419	+	234	WP_012601717	hypothetical protein	-
VJF81_RS00435	68416..68814	-	399	WP_309388060	shufflon protein C	-
VJF81_RS00440	68993..69478	-	486	WP_236926543	hypothetical protein	-
VJF81_RS00445	69829..70218	+	390	Protein_88	phage tail protein	-
VJF81_RS00450	70223..71557	-	1335	WP_059327959	shufflon system plasmid conjugative transfer pilus tip adhesin PilV	-
VJF81_RS00455	71554..72261	-	708	WP_202110611	A24 family peptidase	-
VJF81_RS00460	72221..72697	-	477	WP_029391914	lytic transglycosylase domain-containing protein	virB1
VJF81_RS00465	72756..73292	-	537	WP_029391915	type 4 pilus major pilin	-
VJF81_RS00470	73343..74443	-	1101	WP_029391916	type II secretion system F family protein	-
VJF81_RS00475	74445..75953	-	1509	WP_323873227	ATPase, T2SS/T4P/T4SS family	virB11
VJF81_RS00480	76050..76646	-	597	WP_029391917	hypothetical protein	-
VJF81_RS00485	76742..77296	-	555	WP_021532699	hypothetical protein	-
VJF81_RS00490	77345..77797	-	453	WP_029391918	type IV pilus biogenesis protein PilP	-
VJF81_RS00495	77787..79082	-	1296	WP_021532697	type 4b pilus protein PilO2	-
VJF81_RS00500	79103..80722	-	1620	WP_021532696	PilN family type IVB pilus formation outer membrane protein	-

Host bacterium

• ID: 20044
• GenBank: NZ_CP141229
• Plasmid name: HBI03|unnamed
• Incompatibility group: IncB/O/K/Z
• Plasmid size: 85954 bp
• Coordinate of oriT [Strand]: 24085..24157 [-]
• Host baterium: Escherichia sp. HBI03

Cargo genes

• Drug resistance gene: blaCMY-2
• Virulence gene: -
• Metal resistance gene: -
• Degradation gene: -
• Symbiosis gene: -
• Anti-CRISPR: -