Detailed information of oriT

oriT

The information of the oriT region


oriTDB ID   119609
Name   oriT_pNCDO700A in_silico
Organism   Lactococcus cremoris strain NCDO700
Sequence Completeness      -
NCBI accession of oriT (coordinates [strand])   NZ_CP069180 (3166..3203 [-], 38 nt)
oriT length   38 nt
IRs (inverted repeats)      1..7, 19..25  (ACACCAC..GTGGTGT)
Location of nic site      _
Conserved sequence flanking the
  nic site    		  _
Note   Predicted by oriTfinder 2.0

  oriT sequence  


Download         Length: 38 nt

>oriT_pNCDO700A
ACACCACCCAATTTTGGAGTGGTGTGTAAGTGCGCATT

Visualization of oriT structure

  oriT secondary structure

Predicted by RNAfold.

Download structure file


Relaxase

ID   12388 GenBank   WP_010890626
Name   mobQ_LLNCDO700_RS12980_pNCDO700A insolico UniProt ID   A0A8B3ETK9
Length   680 a.a. PDB ID   
Note   Predicted by oriTfinder 2.0

  Relaxase protein sequence


Download         Length: 680 a.a.        Molecular weight: 79275.45 Da        Isoelectric Point: 6.7052

>WP_010890626.1 MULTISPECIES: MobQ family relaxase [Lactococcus]
MAIFHMNFSNISAGKGRSAVASASYRSGEKLYSEMENKTYFYNRSVMPESFILLPENAPEWAKDRQKLWN
EVEAVDRKVNSRYAKEFNVALPIELSEDEQKELLTEYVQKIFVDKGMVADVAIHRDHDENPHAHVMLTNR
PFNADGSWGQKAKKEYILDENGNKTYTANGHARSRKIWLVDWDKVGKVEEWRKAWADHVNSVFQEKNIDE
RISEKTLEAQGINDIATQHVGVTGNRDERAEFNKLVLENRQHKAELENLDEKINNELKVKQLKNFYSFNE
KKVIAELSKELHTFIDLEHLEEKNKMLFNWKNSVLIKQIVGKDVSEELNKVSRQEFSLDNANRLIDKVVD
RSIKAMYPTVDSNAFSMPEKRQLIRETESENKVFTGAELDDRLSMIRADSLNRQIVALTKRPFTSLTMLS
KQEKYHIDNINNVLAYQGYNYENIEMSHGKILRNYESEEFSKELDIIQRSLKQINSIEEVRKIVEQQYSV
VLGTIFPDSELNKLSLVEKEKTYNAVIYFNPTIKKLTQSELENIVKEPPILFSTKEHNQGLSYLAGRIRL
EDINNKHLTRVLKFEGTKKLFIGEAKADKNIDPELLKEATNRNDKQSYKNEFYRDKNMENYQSVAYSDTS
PAEYMNRLFSGVLISVLYNNENQHKKGLEEVEWDMERKHREHTKTGGLSR

  Protein domains


Predicted by InterproScan.

MobA/MobL family (MobA_MobL)

(17-237)

Nicking enzyme C-terminal middle helical domain (NES_C_h)

(276-383)


  Protein structure


Source ID Structure
AlphaFold DB A0A8B3ETK9


T4CP

ID   14547 GenBank   WP_010890635
Name   t4cp2_LLNCDO700_RS13185_pNCDO700A insolico UniProt ID   _
Length   530 a.a. PDB ID   _
Note   Predicted by oriTfinder 2.0

  T4CP protein sequence


Download         Length: 530 a.a.        Molecular weight: 59615.30 Da        Isoelectric Point: 4.8001

>WP_010890635.1 MULTISPECIES: VirD4-like conjugal transfer protein, CD1115 family [Lactococcus]
MNGTILGVLDNKIIYQDNTTKPNRNVMVIGGSGSYKTQSVVITNLFNETKNSIVVTDPKGELYEKTAGIK
LAQGYEVHVVNFANMAHSDRYNPFDYIERDIQAESVATKIVQSENAEGKKDVWFSTQRQLLKALILFVMK
ERSPEQRNLAGVINVLQTFDSEPINKDENSDLDNLFLALKITHPARIAYELGFKKAKGDMKASIISSLLA
TISKFTDEEVSNFTSISDFHLQDIGRKKIVLYVIIPVMDNTYESFINLFFSQMFDELYKLASSNGAKLPQ
EVDFILDEFVNLGKFPKYEEFLATCRGYGIGVTTICQTLTQLQSLYGKEKAESILGNHAVKICLNASNEA
TAKYFSELLGKSTVKVETGSESTSHSKETSTSKSDSYSYTSRQLMTPDEIIRMPDTQSLLIFTNQKPIKA
TKAFQFKLFPDADSKVKLEQNKYVGITSKSQLEKYNDLSVKWEEKLQSLKNITVTEEEEKDLQDNIDSDL
EAMNNENSGVDNSTTETDKIAETEAKEVQAEEKNFDQVVL

  Protein domains


Predicted by InterproScan.

Type IV secretory system Conjugative DNA transfer (T4SS-DNA_transf)

(25-452)

  Protein structure



No available structure.




Host bacterium

ID   20039 GenBank   NZ_CP069180
Plasmid name   pNCDO700A Incompatibility group   -
Plasmid size   71195 bp Coordinate of oriT [Strand]   3166..3203 [-]
Host baterium   Lactococcus cremoris strain NCDO700

Cargo genes

Drug resistance gene   -
Virulence gene   -
Metal resistance gene   -
Degradation gene   -
Symbiosis gene   -
Anti-CRISPR   -