Detailed information of oriT

oriT

The information of the oriT region


oriTDB ID   119578
Name   oriT_pLAU_KAM9_OXA48 in_silico
Organism   Klebsiella aerogenes strain ST143
Sequence Completeness      -
NCBI accession of oriT (coordinates [strand])   NZ_MN792918 (12677..12782 [+], 106 nt)
oriT length   106 nt
IRs (inverted repeats)      30..35, 41..46  (CCGCCG..CGGCGG)
Location of nic site      _
Conserved sequence flanking the
  nic site    		  _
Note   Predicted by oriTfinder 2.0

  oriT sequence  


Download         Length: 106 nt

>oriT_pLAU_KAM9_OXA48
AGTACGGGACAAGATGTGTTTTTGGAGTACCGCCGACACGCGGCGGCCGTCCAAAAACGTCTTGGTCAGGGCAAGCCCCGACACCCCCTAACGAGGTTAGCTATCT

Visualization of oriT structure

  oriT secondary structure

Predicted by RNAfold.

Download structure file


Relaxase

ID   12374 GenBank   WP_004187323
Name   traI_H5G38_RS00130_pLAU_KAM9_OXA48 insolico UniProt ID   A0A3U8KUL3
Length   659 a.a. PDB ID   
Note   Predicted by oriTfinder 2.0

  Relaxase protein sequence


Download         Length: 659 a.a.        Molecular weight: 75179.89 Da        Isoelectric Point: 9.9948

>WP_004187323.1 MULTISPECIES: TraI/MobA(P) family conjugative relaxase [Enterobacterales]
MVIPKIIEGRRDKKSSFGQLIKYMADKPSQELTDTVQPTPETALAVKSDMFEGLNNYLTRKQKVISTPVD
VEPGVQRVVVGDVTCQYNTFSLDGAAQEMNSVSQQSTRCKDPVMHYVLSWPDYEKPNDDQVFDSVKFTLA
SMGMSDHQYVAAIHRDTDNLHVHVAVNRINPQTYKAASSSFTKDTLHQACRLLELKNGWSHSNGAYVVND
RQQIVRNPHSKKERGNWRSLDRINKMENKEGVETLYRYIVGDEQVGGSRQNLIHVSAGLREAKSWDDVHK
TFADIGLRVEKAQGKKGYVITHEHQNQKTAVKASLVFNKAQYTLKSMEERFGEYQPSHIEPAKVSVFKTA
YTPGAYRRDANKRLQRKIERAEERMLLKGRYRAYRNNLPIYSPDKDRIADEYRKIAQHTRLVKNNVRHSV
SDPHTRKLMYNLAEFKRLQAVANLRLSLREERNGFRAANPRLSYREWVEQEALKGDKAALSQMRGFAYSS
RKKEKYKQQLVEQIGFNRTFNAITSHDRDDVAVMASARHGVKPRLLKDGTVIFERDGKPVAADRGHIVLT
ESNGIDKEKTADLAIALTIAGKAKSVRVDGDGEFKELCCNRIVDAAVNHNHPVAQGITFTDAAQQAYAQN
EKHRLIREQNNSKNEMQFRSESDDKFNPK

  Protein domains


Predicted by InterproScan.

Relaxase/Mobilisation nuclease domain (Relaxase)

(86-334)


  Protein structure


Source ID Structure
AlphaFold DB A0A3U8KUL3


T4CP

ID   14523 GenBank   WP_004206885
Name   t4cp2_H5G38_RS00245_pLAU_KAM9_OXA48 insolico UniProt ID   _
Length   695 a.a. PDB ID   _
Note   Predicted by oriTfinder 2.0

  T4CP protein sequence


Download         Length: 695 a.a.        Molecular weight: 79538.91 Da        Isoelectric Point: 4.8317

>WP_004206885.1 MULTISPECIES: conjugal transfer protein TrbC [Enterobacterales]
MQQESVDSKKVLRPDGSFMEWFLTPNVQFGLLAILTVLGLFLPFTMLLSILILPPLMVAFTDRKFRPPLR
MPKDCEMFDDTLTTETQAEYRLGPIRIPHKVRERKKAKGILYVGYERGRLFGRELWLNMTDLLRHMVFFG
TTGSGKTETFYGFIVNFLLWCRGYCLSDGKADNKLAFATWSLARRFGREDDYYVLNLLTGSIDRFVNLVK
QESIPAQSNSVNLFSVAPPTFIIQLMESMLPQVGGDSAQWQDTAKAMMSALINALCYKRARGELLLSQRT
IQKNMSLPAMAALYVEAKKNGWHSEGYAALESYLENTPGFLLANAEYPETWEARAFEQHNYMSRQFLKTL
SLFNETYGHVFPEDSGDIRMDDIFHNDRILIVMIPSLELSRGEAATLGRLYVTLQRMTISKDLGYQLEGK
KEEVLLTHALNNQAPYGLIYDELGQYFTSGMDTLSAQMRSLEKMGVFSSQDHPSLARGANGEVDSLIANT
RVKYFESIEDRKTFEILRETVGQDYYSELSGQEAEHGTFSKTVREGDLYQIREKDRVNIRELRKQTEGQG
VISFQDALVRSAAFYIPDNEKFSSELPMRINRFIEVLPPSPATLYSIYPERKDDELAETNPDAMRFPPIK
LFGYDKAANSLLEKIEVFYELQCGAISPEEMSVCLFELFAEWLDGTETDDVLYHQEDDLFPMIEM

  Protein domains



No domain identified.


  Protein structure



No available structure.




T4SS

T4SS were predicted by using oriTfinder2.

Region 1: 15578..42786

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
H5G38_RS00110 (pLAU_KAM9_OXA48_00022) 10662..10973 + 312 WP_004187333 hypothetical protein -
H5G38_RS00115 (pLAU_KAM9_OXA48_00023) 11106..11642 + 537 WP_004187332 hypothetical protein -
H5G38_RS00120 (pLAU_KAM9_OXA48_00025) 12144..12539 - 396 WP_019725163 hypothetical protein -
H5G38_RS00415 12538..13101 + 564 WP_020277892 plasmid mobilization protein MobA -
H5G38_RS00130 (pLAU_KAM9_OXA48_00027) 13088..15067 + 1980 WP_004187323 TraI/MobA(P) family conjugative relaxase -
H5G38_RS00135 (pLAU_KAM9_OXA48_00028) 15081..15581 + 501 WP_004187320 DotD/TraH family lipoprotein -
H5G38_RS00140 (pLAU_KAM9_OXA48_00029) 15578..16357 + 780 WP_004187315 type IV secretory system conjugative DNA transfer family protein traI
H5G38_RS00145 (pLAU_KAM9_OXA48_00030) 16368..17531 + 1164 WP_004187313 plasmid transfer ATPase TraJ virB11
H5G38_RS00150 (pLAU_KAM9_OXA48_00031) 17521..17781 + 261 WP_004187310 IcmT/TraK family protein traK
H5G38_RS00405 (pLAU_KAM9_OXA48_00032) 17806..21108 + 3303 WP_219724342 LPD7 domain-containing protein -
H5G38_RS00160 (pLAU_KAM9_OXA48_00033) 21074..21586 + 513 WP_011091071 hypothetical protein traL
H5G38_RS00165 (pLAU_KAM9_OXA48_00034) 21587..21784 - 198 WP_004187464 Hha/YmoA family nucleoid-associated regulatory protein -
H5G38_RS00170 (pLAU_KAM9_OXA48_00035) 21771..22181 - 411 WP_004187465 H-NS family nucleoid-associated regulatory protein -
H5G38_RS00175 (pLAU_KAM9_OXA48_00036) 22180..22962 + 783 WP_015058941 DotI/IcmL family type IV secretion protein traM
H5G38_RS00180 (pLAU_KAM9_OXA48_00037) 22971..24122 + 1152 WP_004187471 DotH/IcmK family type IV secretion protein traN
H5G38_RS00185 (pLAU_KAM9_OXA48_00038) 24134..25483 + 1350 WP_004187474 conjugal transfer protein TraO traO
H5G38_RS00190 (pLAU_KAM9_OXA48_00039) 25495..26199 + 705 WP_015060002 conjugal transfer protein TraP traP
H5G38_RS00195 (pLAU_KAM9_OXA48_00040) 26223..26753 + 531 WP_004187478 conjugal transfer protein TraQ traQ
H5G38_RS00200 (pLAU_KAM9_OXA48_00041) 26770..27159 + 390 WP_004187479 DUF6750 family protein traR
H5G38_RS00205 (pLAU_KAM9_OXA48_00042) 27205..27699 + 495 WP_004187480 hypothetical protein -
H5G38_RS00210 (pLAU_KAM9_OXA48_00043) 27696..30746 + 3051 WP_004187482 hypothetical protein traU
H5G38_RS00215 (pLAU_KAM9_OXA48_00044) 30743..31951 + 1209 WP_011091082 conjugal transfer protein TraW traW
H5G38_RS00220 (pLAU_KAM9_OXA48_00045) 31948..32544 + 597 WP_015060003 hypothetical protein -
H5G38_RS00225 (pLAU_KAM9_OXA48_00046) 32537..34732 + 2196 WP_015062834 DotA/TraY family protein traY
H5G38_RS00230 (pLAU_KAM9_OXA48_00047) 34734..35387 + 654 WP_015060005 hypothetical protein -
H5G38_RS00235 (pLAU_KAM9_OXA48_00048) 35456..35698 + 243 WP_023893611 IncL/M type plasmid replication protein RepC -
H5G38_RS00240 (pLAU_KAM9_OXA48_00049) 35982..37049 + 1068 WP_019725043 plasmid replication initiator RepA -
H5G38_RS00425 38272..38367 + 96 WP_004206884 DinQ-like type I toxin DqlB -
H5G38_RS00245 (pLAU_KAM9_OXA48_00050) 38418..40505 - 2088 WP_004206885 conjugal transfer protein TrbC -
H5G38_RS00250 (pLAU_KAM9_OXA48_00051) 40518..41468 - 951 WP_004206886 DsbC family protein trbB
H5G38_RS00255 (pLAU_KAM9_OXA48_00052) 41479..42786 - 1308 WP_015059988 hypothetical protein trbA
H5G38_RS00260 (pLAU_KAM9_OXA48_00053) 42786..43142 - 357 WP_015586032 lytic transglycosylase domain-containing protein -
H5G38_RS00265 (pLAU_KAM9_OXA48_00054) 43286..43681 - 396 WP_015059989 DUF1496 domain-containing protein -
H5G38_RS00270 (pLAU_KAM9_OXA48_00055) 43749..44183 + 435 Protein_55 CPBP family intramembrane glutamate endopeptidase -
H5G38_RS00275 (pLAU_KAM9_OXA48_00056) 44198..45406 - 1209 WP_001206290 IS4-like element IS10A family transposase -
H5G38_RS00280 (pLAU_KAM9_OXA48_00057) 45709..46620 + 912 WP_015586033 LysR family transcriptional regulator -
H5G38_RS00285 (pLAU_KAM9_OXA48_00058) 46922..47719 - 798 WP_015059991 OXA-48 family carbapenem-hydrolyzing class D beta-lactamase OXA-48 -

Host bacterium

ID   20008 GenBank   NZ_MN792918
Plasmid name   pLAU_KAM9_OXA48 Incompatibility group   IncL/M
Plasmid size   61913 bp Coordinate of oriT [Strand]   12677..12782 [+]
Host baterium   Klebsiella aerogenes strain ST143

Cargo genes

Drug resistance gene   blaOXA-48
Virulence gene   -
Metal resistance gene   -
Degradation gene   -
Symbiosis gene   -
Anti-CRISPR   -