Detailed information of oriT

oriT

The information of the oriT region


oriTDB ID   119576
Name   oriT_pEa1631 in_silico
Organism   Klebsiella aerogenes
Sequence Completeness      -
NCBI accession of oriT (coordinates [strand])   NZ_MG516907 (28558..28663 [+], 106 nt)
oriT length   106 nt
IRs (inverted repeats)      30..35, 41..46  (CCGCCG..CGGCGG)
Location of nic site      _
Conserved sequence flanking the
  nic site    		  _
Note   Predicted by oriTfinder 2.0

  oriT sequence  


Download         Length: 106 nt

>oriT_pEa1631
AGTACGGGACAAGATGTGTTTTTGGAGTACCGCCGACACGCGGCGGCCGTCCAAAAACGTCTTGGTCAGGGCAAGCCCCGACACCCCCTAACGAGGTTAGCTATCT

Visualization of oriT structure

  oriT secondary structure

Predicted by RNAfold.

Download structure file


Relaxase

ID   12372 GenBank   WP_050868819
Name   traI_HTQ77_RS00255_pEa1631 insolico UniProt ID   A0A142BMF1
Length   659 a.a. PDB ID   
Note   Predicted by oriTfinder 2.0

  Relaxase protein sequence


Download         Length: 659 a.a.        Molecular weight: 75234.98 Da        Isoelectric Point: 10.0285

>WP_050868819.1 MULTISPECIES: TraI/MobA(P) family conjugative relaxase [Enterobacterales]
MVIPKIIEGRRDKKSSFGQLIKYMADKPSQELTDTVQPTPETALAVKSDMFEGLNNYLTRKQKVISTPVD
VEPGVQRVVVGDVTCQYNTFSLDGAAQEMNSVSQQSTRCKDPVMHYVLSWPDYEKPNDDQVFDSVKFTLA
SMGMSDHQYVAAIHRDTDNLHVHVAVNRINPQTYKAASSSFTKDTLHQACRLLELKNGWSHSNGAYVVND
RQQIVRNPHSKKERGNWRSLDRINKMENKEGVETLYRYIVGDEQVGGSRQNLIHVSAGLREAKSWDDVHK
TFADIGLRVEKAQGKKGYVITHEHQNQKTAVKASLVFNKAQYTLKSMEERFGEYQPSHIEPAKVSVFKTA
YTPGAYRRDANKRLQRKIERAEERMLLKGRYRAYRNNLPVYSPDKDRIADEYRKIAQHTRLVKNNVRHSV
SDPHTRKLMYNLAEFKRLQAVANLRLSLREERNGFRAANPRLSYREWVEQEALKGDKAALSQMRGFAYSS
RKKEKYKQQLVEQIGFNRTFNAITSHDRDDVAVMASARHGVKPRLLKDGTVIFERDGKPVAADRGHIVLT
ESNGIDKEKTADLAIALTIAGKAKSVRVDGDGEFKELCCNRIVDAAVNHNHPVAQGITFTDAAQQAYAQN
EKHRLIREQNNSKNEMQFRSERDDKFNPK

  Protein domains


Predicted by InterproScan.

Relaxase/Mobilisation nuclease domain (Relaxase)

(86-334)


  Protein structure


Source ID Structure
AlphaFold DB A0A142BMF1


T4CP

ID   14520 GenBank   WP_011091092
Name   t4cp2_HTQ77_RS00505_pEa1631 insolico UniProt ID   _
Length   695 a.a. PDB ID   _
Note   Predicted by oriTfinder 2.0

  T4CP protein sequence


Download         Length: 695 a.a.        Molecular weight: 79491.81 Da        Isoelectric Point: 4.8270

>WP_011091092.1 MULTISPECIES: hypothetical protein [Gammaproteobacteria]
MQQESVDSKKVIRPDGSFMEWFLTPNVQFGLLAILTVLGLFLPFTMLLSILILPPLMVAFTDRKFRPPLR
MPKDCEMFDDTLTTETQAEYRLGPIRIPHKVRERKKAKGILYVGYERGRLFGRELWLNMTDLLRHMVFFG
TTGSGKTETFYGFIVNFLLWCRGYCLSDGKADNKLAFATWSLARRFGREDDYYVLNLLTGSIDRFVNLVK
QESIPAQSNSVNLFSVAPPTFIIQLMESMLPQVGGDSAQWQDTAKAMMSALINALCYKRARGELLLSQRT
IQKNMSLPAMAALYVEAKKNGWHSEGYAALESYLENTPGFLLANAEYPETWEARAFEQHNYMSRQFLKTL
SLFNETYGHVFPEDSGDIRMDDIFHNDRILIVMIPSLELSRGEAATLGRLYVTLQRMTISKDLGYQLEGK
KEEVLLTHALNNQAPYGLIYDELGQYFTSGMDTLSAQMRSLEKMGVFSSQDHPSLARGANGEVDSLIANT
RVKYFESIEDRKTFEILRETVGQDYYSELSGQEAEHGTFSKTVREGDLYQIREKDRVNIRELRKQTEGQG
VISFQDALVRSAAFYIPDNEKFSSELPMRINRFIEVLPPSPATLYSIYPERKDDELAETNPDAMHFPPIK
LFGYDKAANSLLEKIEVFYELQCGAISPEEMSVCLFELFADWLDGTDTDDVLYHQEDDLFPMIEM

  Protein domains



No domain identified.


  Protein structure



No available structure.




T4SS

T4SS were predicted by using oriTfinder2.

Region 1: 31459..50607

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HTQ77_RS00235 26543..26854 + 312 WP_011091065 hypothetical protein -
HTQ77_RS00240 26987..27523 + 537 WP_011091066 hypothetical protein -
HTQ77_RS00245 28024..28389 - 366 WP_011091067 hypothetical protein -
HTQ77_RS00535 28404..28982 + 579 WP_223811519 plasmid mobilization protein MobA -
HTQ77_RS00255 28969..30948 + 1980 WP_050868819 TraI/MobA(P) family conjugative relaxase -
HTQ77_RS00260 30962..31462 + 501 WP_004187320 DotD/TraH family lipoprotein -
HTQ77_RS00265 31459..32238 + 780 WP_015063359 type IV secretory system conjugative DNA transfer family protein traI
HTQ77_RS00270 32249..33412 + 1164 WP_004187313 plasmid transfer ATPase TraJ virB11
HTQ77_RS00275 33402..33662 + 261 WP_004187310 IcmT/TraK family protein traK
HTQ77_RS00540 33687..36944 + 3258 WP_015063358 LPD7 domain-containing protein -
HTQ77_RS00285 36868..37422 + 555 WP_011154467 hypothetical protein traL
HTQ77_RS00290 37423..37620 - 198 WP_004187464 Hha/YmoA family nucleoid-associated regulatory protein -
HTQ77_RS00295 37607..38017 - 411 WP_004187465 H-NS family nucleoid-associated regulatory protein -
HTQ77_RS00300 38016..38798 + 783 WP_011091073 DotI/IcmL family type IV secretion protein traM
HTQ77_RS00305 38807..39958 + 1152 WP_011091074 DotH/IcmK family type IV secretion protein traN
HTQ77_RS00310 39970..41319 + 1350 WP_011091075 conjugal transfer protein TraO traO
HTQ77_RS00315 41331..42035 + 705 WP_011091076 conjugal transfer protein TraP traP
HTQ77_RS00320 42059..42589 + 531 WP_011091077 conjugal transfer protein TraQ traQ
HTQ77_RS00325 42606..42995 + 390 WP_011091078 DUF6750 family protein traR
HTQ77_RS00330 43041..43535 + 495 WP_011091080 hypothetical protein -
HTQ77_RS00335 43532..46582 + 3051 WP_011091081 hypothetical protein traU
HTQ77_RS00340 46579..47787 + 1209 WP_011091082 conjugal transfer protein TraW traW
HTQ77_RS00345 47784..48434 + 651 WP_011091083 hypothetical protein -
HTQ77_RS00350 48427..50607 + 2181 WP_004187492 DotA/TraY family protein traY
HTQ77_RS00355 50610..51263 + 654 WP_011091084 hypothetical protein -
HTQ77_RS00360 51320..51568 + 249 WP_015062836 IncL/M type plasmid replication protein RepC -
HTQ77_RS00365 51854..52921 + 1068 WP_050868820 plasmid replication initiator RepA -
HTQ77_RS00370 53976..54680 + 705 WP_001067834 IS6-like element IS26 family transposase -

Host bacterium

ID   20006 GenBank   NZ_MG516907
Plasmid name   pEa1631 Incompatibility group   IncL/M
Plasmid size   85489 bp Coordinate of oriT [Strand]   28558..28663 [+]
Host baterium   Klebsiella aerogenes

Cargo genes

Drug resistance gene   blaIMP-4, catB3, qacE, sul1, mph(A), aac(3)-IId, blaTEM-1B
Virulence gene   -
Metal resistance gene   -
Degradation gene   -
Symbiosis gene   -
Anti-CRISPR   -