Detailed information of oriT
oriT
The information of the oriT region
| oriTDB ID | 119561 |
| Name | oriT_FH-1|unnamed  |
| Organism | Klebsiella variicola strain FH-1 |
| Sequence Completeness | - |
| NCBI accession of oriT (coordinates [strand]) | NZ_CP054255 (75037..75086 [+], 50 nt) |
| oriT length | 50 nt |
| IRs (inverted repeats) | 7..14, 17..24 (GCAAAATT..AATTTTGC) |
| Location of nic site | 33..34 |
| Conserved sequence flanking the nic site |
GGTGTGGTGA |
| Note | Predicted by oriTfinder 2.0 |
oriT sequence
Download Length: 50 nt
>oriT_FH-1|unnamed
AAATCTGCAAAATTTTAATTTTGCGTGGGGTGTGGTGATTTTGTGGTGAG
AAATCTGCAAAATTTTAATTTTGCGTGGGGTGTGGTGATTTTGTGGTGAG
Visualization of oriT structure
oriT secondary structure
Predicted by RNAfold.
Download structure file
Relaxase
| ID | 12361 | GenBank | WP_173818510 |
| Name | traI_HL854_RS26940_FH-1|unnamed |
UniProt ID | _ |
| Length | 1753 a.a. | PDB ID | |
| Note | Predicted by oriTfinder 2.0 | ||
Relaxase protein sequence
Download Length: 1753 a.a. Molecular weight: 190863.86 Da Isoelectric Point: 6.1411
>WP_173818510.1 MULTISPECIES: conjugative transfer relaxase/helicase TraI [Klebsiella]
MMSIGSVKSAGSAGNYYTDKDNYYVIGSMDERWQGKGAEALGIDGKAVDKTLFTELLKGKLPDGSDLTRI
QDGANKHRPGYDLTFSAPKSVSVMAMLGGDKRLIDAHNQAVTEAVRQLETLAATRVMTDGKSETVLTGNL
IVAKFNHDTNRNQEPQIHTHAVVINATQNGDKWQTLGTDKIGKTGFIENVYANQIAFGKLYREAFKPLVE
KLGYETEVVGKHGMWEMKGVPVEPFSTRTQEVREAAGPDASLKSRDVAALDTRKSKEAIDPAEKMVEWMN
TLKETGFDIRGYREAADARAAELARAPAAPVNTDGPDITDVVTKAIAGLSDRKVQFTYADLLARTVGQLE
AKDGVFELARKGIDAAIEREQLIPLDREKGLFTSNIHVLDELAVKALSQEVQRQNHVSVTPDASVVRQVP
FSDAVSVLAQDRPVMGIVSGQGGATGQRERVAELTLMAREQGRDVHILAADNRSRDFLASDVRLAGETVT
GKSALQDGTAFIPGGTLIVDQAEKLSLKETISLLDGAMRHNVQVLLSDSGKRSGTGSALTVLKDSGVNTY
RWQGGHQTTADIISEPDKGARYSRLAQEFAVSVREGQESVAQISGTREQSVLNGLIRDSLRHEGVLGEKD
TTITSLTPVWLDSKSRGVRDYYREGMVMERWDPENRTHDRFVIDRVTASSNMLTLKDREGVRLDLKVSAV
DSQWTLFRADTLPVAEGERLAVLGKIPDTRLKGGESITVMKVEDGQLTVQRPGQKTTQTLAVGAGVFDGI
KIGHGWVESPGRSVSETATVFASVTQRELDNATLNQLAQSGSHLRLYSAQDAARTTEKLSRHTAFSVVSE
QLKTRSGETDLDAAIAQQKAGLHTPAEQAIHLSIPLLESQDLTFTRPQLLATALETGGDKVPMWSIEMTI
KKQIKSGQLLNVGVSPGHGNDLLISRQTWDAEKSILTRVLEGKDAVAPLMDRVPASLMTDLTAGQRAATR
MILETPDRFTVVQGYAGVGKTTQFRAVMSAISLLPEETRPHVIGLGPTHRAVGEMQSAGVDARTTASFLH
DTQLLQRNGQTPDFSNTLFLLDESSMVGLSDTAKALSLIAAGGGRAVLSGDTDQLQSISPGQPFRLMQQR
SAADVAIMKEIVRQVPELRPAVYSLIDRDIDRALATIEQVTPERVPRKEGAWVPGSSVVEFTPTQEEEIR
KALSKGESLPAGQPATLYEALVKDYTGRTPEAQSQTLIITHLNNDRRALNGLIHEARRENGETGREEVTL
PVLVTSNIRDGELRKLSTWTAHKGAVALVDNVYHRIDNVDKDNQLITLTDSEGKERYISPREASAEGVTL
YRQEEITVSQGDRMRFSKSDPERGYVANSVWEVQSVSGDSVTLSDGKTTRTLTPKADEAQQHIDLAYAIT
AHGAQGASEPYAIALEGMVGARKLMASFESAYVGLSRMKQHVQVYTDRREGWIKAIQHSPEKATAHDILE
PRNDRAVKTADLLFGRARPLDETAAGRAALQQSGLAQGSSPGKFISPGKKYPQPHVALPAFDKNGKAAGI
WLSPLTDRDGRLEAIGGEGRIMGNEDARFVALQNSRNGESLLAGNIGEGVRMARDNPDTGVVVRLAGDDR
PWNPGAITGGRVWAEQAPVAPVPQAGADIILPPEVLAQRAAEEQQRREMEKQAEQTAREVAGEARKAGEP
VERVKEVIGDVIRGLERDRPGTEKTTLPDDPKFRRQEAAIQQVASETLQRDRLQAVERDMVRDLNREKTL
GGD
MMSIGSVKSAGSAGNYYTDKDNYYVIGSMDERWQGKGAEALGIDGKAVDKTLFTELLKGKLPDGSDLTRI
QDGANKHRPGYDLTFSAPKSVSVMAMLGGDKRLIDAHNQAVTEAVRQLETLAATRVMTDGKSETVLTGNL
IVAKFNHDTNRNQEPQIHTHAVVINATQNGDKWQTLGTDKIGKTGFIENVYANQIAFGKLYREAFKPLVE
KLGYETEVVGKHGMWEMKGVPVEPFSTRTQEVREAAGPDASLKSRDVAALDTRKSKEAIDPAEKMVEWMN
TLKETGFDIRGYREAADARAAELARAPAAPVNTDGPDITDVVTKAIAGLSDRKVQFTYADLLARTVGQLE
AKDGVFELARKGIDAAIEREQLIPLDREKGLFTSNIHVLDELAVKALSQEVQRQNHVSVTPDASVVRQVP
FSDAVSVLAQDRPVMGIVSGQGGATGQRERVAELTLMAREQGRDVHILAADNRSRDFLASDVRLAGETVT
GKSALQDGTAFIPGGTLIVDQAEKLSLKETISLLDGAMRHNVQVLLSDSGKRSGTGSALTVLKDSGVNTY
RWQGGHQTTADIISEPDKGARYSRLAQEFAVSVREGQESVAQISGTREQSVLNGLIRDSLRHEGVLGEKD
TTITSLTPVWLDSKSRGVRDYYREGMVMERWDPENRTHDRFVIDRVTASSNMLTLKDREGVRLDLKVSAV
DSQWTLFRADTLPVAEGERLAVLGKIPDTRLKGGESITVMKVEDGQLTVQRPGQKTTQTLAVGAGVFDGI
KIGHGWVESPGRSVSETATVFASVTQRELDNATLNQLAQSGSHLRLYSAQDAARTTEKLSRHTAFSVVSE
QLKTRSGETDLDAAIAQQKAGLHTPAEQAIHLSIPLLESQDLTFTRPQLLATALETGGDKVPMWSIEMTI
KKQIKSGQLLNVGVSPGHGNDLLISRQTWDAEKSILTRVLEGKDAVAPLMDRVPASLMTDLTAGQRAATR
MILETPDRFTVVQGYAGVGKTTQFRAVMSAISLLPEETRPHVIGLGPTHRAVGEMQSAGVDARTTASFLH
DTQLLQRNGQTPDFSNTLFLLDESSMVGLSDTAKALSLIAAGGGRAVLSGDTDQLQSISPGQPFRLMQQR
SAADVAIMKEIVRQVPELRPAVYSLIDRDIDRALATIEQVTPERVPRKEGAWVPGSSVVEFTPTQEEEIR
KALSKGESLPAGQPATLYEALVKDYTGRTPEAQSQTLIITHLNNDRRALNGLIHEARRENGETGREEVTL
PVLVTSNIRDGELRKLSTWTAHKGAVALVDNVYHRIDNVDKDNQLITLTDSEGKERYISPREASAEGVTL
YRQEEITVSQGDRMRFSKSDPERGYVANSVWEVQSVSGDSVTLSDGKTTRTLTPKADEAQQHIDLAYAIT
AHGAQGASEPYAIALEGMVGARKLMASFESAYVGLSRMKQHVQVYTDRREGWIKAIQHSPEKATAHDILE
PRNDRAVKTADLLFGRARPLDETAAGRAALQQSGLAQGSSPGKFISPGKKYPQPHVALPAFDKNGKAAGI
WLSPLTDRDGRLEAIGGEGRIMGNEDARFVALQNSRNGESLLAGNIGEGVRMARDNPDTGVVVRLAGDDR
PWNPGAITGGRVWAEQAPVAPVPQAGADIILPPEVLAQRAAEEQQRREMEKQAEQTAREVAGEARKAGEP
VERVKEVIGDVIRGLERDRPGTEKTTLPDDPKFRRQEAAIQQVASETLQRDRLQAVERDMVRDLNREKTL
GGD
Protein domains
Predicted by InterproScan.
Protein structure
No available structure.
T4CP
| ID | 14511 | GenBank | WP_228722999 |
| Name | traD_HL854_RS26945_FH-1|unnamed |
UniProt ID | _ |
| Length | 763 a.a. | PDB ID | _ |
| Note | Predicted by oriTfinder 2.0 | ||
T4CP protein sequence
Download Length: 763 a.a. Molecular weight: 85444.47 Da Isoelectric Point: 5.0671
>WP_228722999.1 type IV conjugative transfer system coupling protein TraD [Klebsiella variicola]
MSFNAKDMTQGGQIANMRFRMFGQIANIIFYVLFILFWVLCGLMLMYRLSWQTFVNGCVYWWCTTLGPMR
DIIRSQPVYTIQYYGQSLEYTSEQILADKYTIWCGEQLWTSFVFAAVVSLVICIVTFFIASWVLGRQGKQ
QSEDENTGGRQLSDKPKEVARQMKRDGMASDIKIGDLPILKNSEIQNFCLHGTVGSGKSEVIRRLLNYVR
ARGDMAIIYDRSCEFVKSYYDPSLDKILNPLDSRCAAWDLWKECLTLPDFDNISNTLIPMGTKEDPFWQG
SGRTIFAEGAYLMREDKDRSYEKLVDTMLSIKIDKLRAYLQNTPAANLVEEKIEKTAISIRAVLTNYVKA
IRYLQGIEKNDEPFTIRDWMRGVREDRPNGWLFISSNADTHASLKPVISMWLSIAIRGLLAMGENRNRRV
WIFADELPTLHKLPDLVEILPEARKFGGCYVFGIQSYAQLEDIYGVKPAATLFDVMNTRAFFRSPSKEIA
EFAAGEIGEKEILKASEQYSYGADPVRDGVSTGKEKERETLVSYSDIQTLPDLSCYVTLPGPYPAVKLAL
KYKPRPKIAEGFIQRRLDTRVDARLSALLEAREAEGSLARALFTPDAADTDSHAGEQPEPVSQPAPAEVT
VSPALVKAPPTTKSPAAEPSARAAEPPVLRVTTVPLIKPKAAAAAAAAATASSVGTPAAGGTEQELAQQS
AEQGQEMLPAGMNEDGVIEDMQAYDAWLADEQTQRDMQRREEVNINRSHRHDEQDDVEIGGNF
MSFNAKDMTQGGQIANMRFRMFGQIANIIFYVLFILFWVLCGLMLMYRLSWQTFVNGCVYWWCTTLGPMR
DIIRSQPVYTIQYYGQSLEYTSEQILADKYTIWCGEQLWTSFVFAAVVSLVICIVTFFIASWVLGRQGKQ
QSEDENTGGRQLSDKPKEVARQMKRDGMASDIKIGDLPILKNSEIQNFCLHGTVGSGKSEVIRRLLNYVR
ARGDMAIIYDRSCEFVKSYYDPSLDKILNPLDSRCAAWDLWKECLTLPDFDNISNTLIPMGTKEDPFWQG
SGRTIFAEGAYLMREDKDRSYEKLVDTMLSIKIDKLRAYLQNTPAANLVEEKIEKTAISIRAVLTNYVKA
IRYLQGIEKNDEPFTIRDWMRGVREDRPNGWLFISSNADTHASLKPVISMWLSIAIRGLLAMGENRNRRV
WIFADELPTLHKLPDLVEILPEARKFGGCYVFGIQSYAQLEDIYGVKPAATLFDVMNTRAFFRSPSKEIA
EFAAGEIGEKEILKASEQYSYGADPVRDGVSTGKEKERETLVSYSDIQTLPDLSCYVTLPGPYPAVKLAL
KYKPRPKIAEGFIQRRLDTRVDARLSALLEAREAEGSLARALFTPDAADTDSHAGEQPEPVSQPAPAEVT
VSPALVKAPPTTKSPAAEPSARAAEPPVLRVTTVPLIKPKAAAAAAAAATASSVGTPAAGGTEQELAQQS
AEQGQEMLPAGMNEDGVIEDMQAYDAWLADEQTQRDMQRREEVNINRSHRHDEQDDVEIGGNF
Protein domains
Predicted by InterproScan.
Protein structure
No available structure.
T4SS
T4SS were predicted by using oriTfinder2.
Region 1: 46607..72539
| Locus tag | Coordinates | Strand | Size (bp) | Protein ID | Product | Description |
|---|---|---|---|---|---|---|
| HL854_RS26945 (HL854_27320) | 46607..48898 | - | 2292 | WP_228722999 | type IV conjugative transfer system coupling protein TraD | virb4 |
| HL854_RS27570 | 48972..49067 | - | 96 | Protein_43 | hypothetical protein | - |
| HL854_RS26950 (HL854_27325) | 49260..49991 | - | 732 | WP_004152629 | conjugal transfer complement resistance protein TraT | - |
| HL854_RS26955 (HL854_27330) | 50181..50708 | - | 528 | WP_047665858 | conjugal transfer protein TraS | - |
| HL854_RS26960 (HL854_27335) | 50714..53563 | - | 2850 | WP_173818511 | conjugal transfer mating-pair stabilization protein TraG | traG |
| HL854_RS26965 (HL854_27340) | 53563..54933 | - | 1371 | WP_047665852 | conjugal transfer pilus assembly protein TraH | traH |
| HL854_RS26970 (HL854_27345) | 54920..55342 | - | 423 | WP_049010971 | hypothetical protein | - |
| HL854_RS26975 (HL854_27350) | 55335..55907 | - | 573 | WP_055323574 | type-F conjugative transfer system pilin assembly thiol-disulfide isomerase TrbB | traF |
| HL854_RS26980 (HL854_27355) | 55879..56118 | - | 240 | WP_009309875 | type-F conjugative transfer system pilin chaperone TraQ | - |
| HL854_RS26985 (HL854_27360) | 56129..56881 | - | 753 | WP_047665849 | type-F conjugative transfer system pilin assembly protein TraF | traF |
| HL854_RS26990 (HL854_27365) | 56902..57228 | - | 327 | WP_012539967 | hypothetical protein | - |
| HL854_RS26995 (HL854_27370) | 57239..57466 | - | 228 | WP_047665846 | conjugal transfer protein TrbE | - |
| HL854_RS27000 (HL854_27375) | 57456..57737 | - | 282 | WP_047665844 | hypothetical protein | - |
| HL854_RS27005 (HL854_27380) | 57770..59725 | - | 1956 | WP_065908465 | type-F conjugative transfer system mating-pair stabilization protein TraN | traN |
| HL854_RS27010 (HL854_27385) | 59784..60422 | - | 639 | WP_047667531 | type-F conjugative transfer system pilin assembly protein TrbC | trbC |
| HL854_RS27015 (HL854_27390) | 60435..61424 | - | 990 | WP_046622837 | conjugal transfer pilus assembly protein TraU | traU |
| HL854_RS27020 (HL854_27395) | 61421..61810 | - | 390 | WP_047667530 | hypothetical protein | - |
| HL854_RS27025 (HL854_27400) | 61852..62478 | - | 627 | WP_004152507 | type-F conjugative transfer system protein TraW | traW |
| HL854_RS27030 (HL854_27405) | 62478..62867 | - | 390 | WP_046622835 | type-F conjugative transfer system protein TrbI | - |
| HL854_RS27035 (HL854_27410) | 62867..65506 | - | 2640 | WP_046622834 | type IV secretion system protein TraC | virb4 |
| HL854_RS27040 (HL854_27415) | 65578..65976 | - | 399 | WP_004153071 | hypothetical protein | - |
| HL854_RS27575 | 65984..66226 | - | 243 | WP_074446773 | hypothetical protein | - |
| HL854_RS27050 (HL854_27425) | 66418..66822 | - | 405 | WP_065907749 | hypothetical protein | - |
| HL854_RS27055 (HL854_27430) | 66889..67200 | - | 312 | WP_047665766 | hypothetical protein | - |
| HL854_RS27060 (HL854_27435) | 67201..67419 | - | 219 | WP_014386199 | hypothetical protein | - |
| HL854_RS27065 (HL854_27440) | 67443..67733 | - | 291 | WP_047665765 | hypothetical protein | - |
| HL854_RS27580 | 67762..68160 | - | 399 | WP_223170083 | hypothetical protein | - |
| HL854_RS27075 (HL854_27450) | 68316..68885 | - | 570 | WP_046622829 | type IV conjugative transfer system lipoprotein TraV | traV |
| HL854_RS27080 (HL854_27455) | 68908..69504 | - | 597 | WP_047665764 | conjugal transfer pilus-stabilizing protein TraP | - |
| HL854_RS27085 (HL854_27460) | 69497..70921 | - | 1425 | WP_046622826 | F-type conjugal transfer pilus assembly protein TraB | traB |
| HL854_RS27090 (HL854_27465) | 70921..71661 | - | 741 | WP_013214019 | type-F conjugative transfer system secretin TraK | traK |
| HL854_RS27095 (HL854_27470) | 71648..72214 | - | 567 | WP_047665763 | type IV conjugative transfer system protein TraE | traE |
| HL854_RS27100 (HL854_27475) | 72234..72539 | - | 306 | WP_004178059 | type IV conjugative transfer system protein TraL | traL |
| HL854_RS27105 (HL854_27480) | 72553..72921 | - | 369 | WP_173818512 | type IV conjugative transfer system pilin TraA | - |
| HL854_RS27110 (HL854_27485) | 72975..73346 | - | 372 | WP_071885111 | TraY domain-containing protein | - |
| HL854_RS27115 (HL854_27490) | 73430..74125 | - | 696 | WP_072194100 | transcriptional regulator TraJ family protein | - |
| HL854_RS27120 (HL854_27495) | 74332..74724 | - | 393 | WP_047665760 | conjugal transfer relaxosome DNA-binding protein TraM | - |
| HL854_RS27125 (HL854_27500) | 75231..75638 | + | 408 | WP_223170089 | transglycosylase SLT domain-containing protein | - |
| HL854_RS27130 (HL854_27505) | 75676..76206 | - | 531 | WP_065907732 | antirestriction protein | - |
Host bacterium
| ID | 19991 | GenBank | NZ_CP054255 |
| Plasmid name | FH-1|unnamed | Incompatibility group | IncFIB |
| Plasmid size | 110148 bp | Coordinate of oriT [Strand] | 75037..75086 [+] |
| Host baterium | Klebsiella variicola strain FH-1 |
Cargo genes
| Drug resistance gene | - |
| Virulence gene | - |
| Metal resistance gene | arsH, arsC, arsB, arsA, arsD, arsR |
| Degradation gene | - |
| Symbiosis gene | - |
| Anti-CRISPR | - |