Detailed information of oriT
oriT
The information of the oriT region
| oriTDB ID | 119506 |
| Name | oriT_pEaer-4382s  |
| Organism | Klebsiella aerogenes strain Eaer-4382 |
| Sequence Completeness | - |
| NCBI accession of oriT (coordinates [strand]) | NZ_KY093014 (73473..73522 [-], 50 nt) |
| oriT length | 50 nt |
| IRs (inverted repeats) | 7..14, 17..24 (GCAAAATT..AATTTTGC) |
| Location of nic site | 33..34 |
| Conserved sequence flanking the nic site |
TGTGTGGTGA |
| Note | Predicted by oriTfinder 2.0 |
oriT sequence
Download Length: 50 nt
>oriT_pEaer-4382s
AAATCTGCAAAATTTTAATTTTGCGTAGTGTGTGGTGATTTTGTGGTGAG
AAATCTGCAAAATTTTAATTTTGCGTAGTGTGTGGTGATTTTGTGGTGAG
Visualization of oriT structure
oriT secondary structure
Predicted by RNAfold.
Download structure file
Relaxase
| ID | 12322 | GenBank | WP_116290300 |
| Name | traI_HTK76_RS00625_pEaer-4382s |
UniProt ID | _ |
| Length | 1752 a.a. | PDB ID | |
| Note | Predicted by oriTfinder 2.0 | ||
Relaxase protein sequence
Download Length: 1752 a.a. Molecular weight: 190657.44 Da Isoelectric Point: 6.1039
>WP_116290300.1 MULTISPECIES: conjugative transfer relaxase/helicase TraI [Klebsiella]
MLSFSQVKSAGSAGNYYTEKDNYYVIGSMEERWQGKGAELLGLEGKVDKQVFTELLQGKLPDGSDLTRIQ
DGVNKHRPGYDLTFSAPKSVSMLAMLGGDKRLIDAHNRAVTVALNQVESLASTRVKKDGVSETVLTGNLI
IARFNHDTSRAQDPQIHTHSVVINATQNGDKWLTLASDTVGKTGFSETILANRIAFGKIYQNSLRADVES
MGYKTVDAGRNGMWEMEGVPVESFSTRSQELREAAGPDASLKSRDVAALDTRKSKEAIDPAEKMVEWMNT
LKETGFDIRGYREAADARAAELARAPAAPVNTDGPDITDVVTKAIAGLSDRKVQFTYADLLARTVGQLEA
KDGVFELARKGINAAIEREQLIPLDREKGLFTSNIHVLDELAVKALSQEVQRQNHVSVTPDASVVRQVPF
SDAVSVLAQDRPVMGIVSGQGGATGQRERVAELTLMAREQGRDVHILAADNRSRDFLAGDVRLAGETVTG
KSALQDGTAFIPGGTLIVDQAEKLSLKETISLLDGAMRHNVQVLLSDSGKRSGTGSALTVLKDSGVNTYR
WQGGHQTTADIISEPDKGARYSRLAQEFAVSVREGQESVAQISGTREQSVLNGLIRDSLRQEGVLGEKDT
TITALTPVWLDSKSRGVRDYYREGMVMERWDPETRTHDRFVIDRVTASSNMLTLKDREGDRLDLKVSAVD
SQWTLFRADTLPVAEGERLAVLGKIPDTRLKGGESVTVMKVEDGQLTVQRPGQKTTQTLAVGAGVFDGIK
VGHGWVESPGRSVSETATVFASVTQRELDNATLNQLAQSGSHLRLYSAQDAARTTEKLSRHTAFSVVSEQ
LKSRSGETDLDAAIAQQKAGLHTPAEQAIHLAIPLLESQDLTFSRPQLLATAMETGGGKVSMADIDTTIQ
TQIRSGQLLNVPVAHGYGNDLLISRQTWDAEKSILTHVLEGKDAVAPLMDRVPASLMTDLTAGQRAATRM
ILESTDRFTVVQGYAGVGKTTQFRAVMSAISLLPEETRPRVIGLAPTHRAVGEMQSAGVEARTTASFLHD
TQLLQRNGQTPDFSNTLFLLDESSMVGLADMAKAHSLIVAGGGRAVSSGDNDQLQPIAPGQPFRLMQQRS
AADVAIMKEIVRQMPELRPAVYSLIERDVHRALTTIEQVTPEQVPRKEGSWAPGSSVVEFTPKQEKAIEK
ALSEGKTLPEGQPATLYEALVKDYTGRTPEAQSQTLVITHLNKDRRALNSLIHDARRENGETGKEEITLP
VLVTSNIRDGELRKLSTWTAHKEAVALVDNVYHRISKVDKDNQLITLTDSEGKERFISPREASAEGVTLY
RQEKITVSQGDRMRFSKSDPERGYVANSIWEVQSVSGDSVTLSDGKITRTLTPKAEQAQQHIDLAYAITA
HGAQGASEPYAIALEGVAGGREQMASFESAYVALSRMKQHVQVYTDNREGWIKAIKNSPEKATAHDILEP
RNDRAVKTADLLFGWARPLDETAAGRAALQQSGLAQGSSPGKFISPGKKYPQPHVALPAFDKNGKAAGIW
LSPLTDRDGRLEAIGGEGRIMGNEDARFVALQNSRNGESLLAGNMGEGVRMARDNPDTGVVVRLAGDDRP
WNPGAMTGGRVWAEPAPVAPVPQAGADIILPPEVLAQRAAEEQQRREMEKQAEQTAREVAGEARKAGEPA
DRVKEVIGDVIRGLERDRPGTEKTTLPDDPQFRRQEAAIQQVASERLQRERLQAVERDMVRDLNREKTLG
GD
MLSFSQVKSAGSAGNYYTEKDNYYVIGSMEERWQGKGAELLGLEGKVDKQVFTELLQGKLPDGSDLTRIQ
DGVNKHRPGYDLTFSAPKSVSMLAMLGGDKRLIDAHNRAVTVALNQVESLASTRVKKDGVSETVLTGNLI
IARFNHDTSRAQDPQIHTHSVVINATQNGDKWLTLASDTVGKTGFSETILANRIAFGKIYQNSLRADVES
MGYKTVDAGRNGMWEMEGVPVESFSTRSQELREAAGPDASLKSRDVAALDTRKSKEAIDPAEKMVEWMNT
LKETGFDIRGYREAADARAAELARAPAAPVNTDGPDITDVVTKAIAGLSDRKVQFTYADLLARTVGQLEA
KDGVFELARKGINAAIEREQLIPLDREKGLFTSNIHVLDELAVKALSQEVQRQNHVSVTPDASVVRQVPF
SDAVSVLAQDRPVMGIVSGQGGATGQRERVAELTLMAREQGRDVHILAADNRSRDFLAGDVRLAGETVTG
KSALQDGTAFIPGGTLIVDQAEKLSLKETISLLDGAMRHNVQVLLSDSGKRSGTGSALTVLKDSGVNTYR
WQGGHQTTADIISEPDKGARYSRLAQEFAVSVREGQESVAQISGTREQSVLNGLIRDSLRQEGVLGEKDT
TITALTPVWLDSKSRGVRDYYREGMVMERWDPETRTHDRFVIDRVTASSNMLTLKDREGDRLDLKVSAVD
SQWTLFRADTLPVAEGERLAVLGKIPDTRLKGGESVTVMKVEDGQLTVQRPGQKTTQTLAVGAGVFDGIK
VGHGWVESPGRSVSETATVFASVTQRELDNATLNQLAQSGSHLRLYSAQDAARTTEKLSRHTAFSVVSEQ
LKSRSGETDLDAAIAQQKAGLHTPAEQAIHLAIPLLESQDLTFSRPQLLATAMETGGGKVSMADIDTTIQ
TQIRSGQLLNVPVAHGYGNDLLISRQTWDAEKSILTHVLEGKDAVAPLMDRVPASLMTDLTAGQRAATRM
ILESTDRFTVVQGYAGVGKTTQFRAVMSAISLLPEETRPRVIGLAPTHRAVGEMQSAGVEARTTASFLHD
TQLLQRNGQTPDFSNTLFLLDESSMVGLADMAKAHSLIVAGGGRAVSSGDNDQLQPIAPGQPFRLMQQRS
AADVAIMKEIVRQMPELRPAVYSLIERDVHRALTTIEQVTPEQVPRKEGSWAPGSSVVEFTPKQEKAIEK
ALSEGKTLPEGQPATLYEALVKDYTGRTPEAQSQTLVITHLNKDRRALNSLIHDARRENGETGKEEITLP
VLVTSNIRDGELRKLSTWTAHKEAVALVDNVYHRISKVDKDNQLITLTDSEGKERFISPREASAEGVTLY
RQEKITVSQGDRMRFSKSDPERGYVANSIWEVQSVSGDSVTLSDGKITRTLTPKAEQAQQHIDLAYAITA
HGAQGASEPYAIALEGVAGGREQMASFESAYVALSRMKQHVQVYTDNREGWIKAIKNSPEKATAHDILEP
RNDRAVKTADLLFGWARPLDETAAGRAALQQSGLAQGSSPGKFISPGKKYPQPHVALPAFDKNGKAAGIW
LSPLTDRDGRLEAIGGEGRIMGNEDARFVALQNSRNGESLLAGNMGEGVRMARDNPDTGVVVRLAGDDRP
WNPGAMTGGRVWAEPAPVAPVPQAGADIILPPEVLAQRAAEEQQRREMEKQAEQTAREVAGEARKAGEPA
DRVKEVIGDVIRGLERDRPGTEKTTLPDDPQFRRQEAAIQQVASERLQRERLQAVERDMVRDLNREKTLG
GD
Protein domains
Predicted by InterproScan.
Protein structure
No available structure.
T4CP
| ID | 14470 | GenBank | WP_020277955 |
| Name | traD_HTK76_RS00620_pEaer-4382s |
UniProt ID | _ |
| Length | 770 a.a. | PDB ID | _ |
| Note | Predicted by oriTfinder 2.0 | ||
T4CP protein sequence
Download Length: 770 a.a. Molecular weight: 85894.89 Da Isoelectric Point: 5.1129
>WP_020277955.1 MULTISPECIES: type IV conjugative transfer system coupling protein TraD [Enterobacteriaceae]
MSFNAKDMTQGGQIANMRFRMFGQIANIIFYVLFILFWVLCGLMLMYRLSWQTFVNGCVYWWCTTLGPMR
DIIRSQPVYTIQYYGQSLEYTSEQILADKYTIWCGEQLWTSFVFAAVVSLVICIVTFFIASWVLGRQGKQ
QSEDENTGGRQLSDKPKEVARQMKRDGMASDIKIGDLPILKNSEIQNFCLHGTVGSGKSEVIRRLLNYVR
ARGDMAIIYDRSCEFVKSYYDPSLDKILNPLDSRCAAWDLWKECLTLPDFDNISNTLIPMGTKEDPFWQG
SGRTIFAEGAYLMREDKDRSYEKLVDTMLSIKIDKLRAYLQNTPAANLVEEKIEKTAISIRAVLTNYVKA
IRYLQGIEKNGEPFTIRDWMRGVREDRPNGWLFISSNADTHASLKPVISMWLSIAIRGLLAMGENRNRRV
WIFADELPTLHKLPDLVEILPEARKFGGCYVFGIQSYAQLEDIYGVKPAATLFDVMNTRAFFRSPSKEIA
EFAAGEIGEKEILKASEQYSYGADPVRDGVSTGKEKERETLVSYSDIQTLPDLSCYVTLPGPYPAVKLAL
KYKPRPKIAEGFIPRTLDARVDARLSALLEAREAEGSLARALFTPDAPASGPADTNSHAGEQPEPVSQPA
PADMTVSPEPVKAPPTTKSPAAEPSVRTTEPSVLRVTTVPLIKPKAAAAAAAASTASSSGAPATAAGGTQ
QELAQQSAEQGQDMLPAGMNEDGVIEDMQAYDAWLADEQTQRDMQRREEVNINHSHRHDEQDDVEIGGNI
MSFNAKDMTQGGQIANMRFRMFGQIANIIFYVLFILFWVLCGLMLMYRLSWQTFVNGCVYWWCTTLGPMR
DIIRSQPVYTIQYYGQSLEYTSEQILADKYTIWCGEQLWTSFVFAAVVSLVICIVTFFIASWVLGRQGKQ
QSEDENTGGRQLSDKPKEVARQMKRDGMASDIKIGDLPILKNSEIQNFCLHGTVGSGKSEVIRRLLNYVR
ARGDMAIIYDRSCEFVKSYYDPSLDKILNPLDSRCAAWDLWKECLTLPDFDNISNTLIPMGTKEDPFWQG
SGRTIFAEGAYLMREDKDRSYEKLVDTMLSIKIDKLRAYLQNTPAANLVEEKIEKTAISIRAVLTNYVKA
IRYLQGIEKNGEPFTIRDWMRGVREDRPNGWLFISSNADTHASLKPVISMWLSIAIRGLLAMGENRNRRV
WIFADELPTLHKLPDLVEILPEARKFGGCYVFGIQSYAQLEDIYGVKPAATLFDVMNTRAFFRSPSKEIA
EFAAGEIGEKEILKASEQYSYGADPVRDGVSTGKEKERETLVSYSDIQTLPDLSCYVTLPGPYPAVKLAL
KYKPRPKIAEGFIPRTLDARVDARLSALLEAREAEGSLARALFTPDAPASGPADTNSHAGEQPEPVSQPA
PADMTVSPEPVKAPPTTKSPAAEPSVRTTEPSVLRVTTVPLIKPKAAAAAAAASTASSSGAPATAAGGTQ
QELAQQSAEQGQDMLPAGMNEDGVIEDMQAYDAWLADEQTQRDMQRREEVNINHSHRHDEQDDVEIGGNI
Protein domains
Predicted by InterproScan.
Protein structure
No available structure.
T4SS
T4SS were predicted by using oriTfinder2.
Region 1: 72915..101960
| Locus tag | Coordinates | Strand | Size (bp) | Protein ID | Product | Description |
|---|---|---|---|---|---|---|
| HTK76_RS00405 | 68935..69291 | + | 357 | WP_019706019 | hypothetical protein | - |
| HTK76_RS00410 | 69352..69564 | + | 213 | WP_019706020 | hypothetical protein | - |
| HTK76_RS00415 | 69575..69799 | + | 225 | WP_014343499 | hypothetical protein | - |
| HTK76_RS00420 | 69880..70200 | + | 321 | WP_004152720 | type II toxin-antitoxin system RelE/ParE family toxin | - |
| HTK76_RS00425 | 70190..70468 | + | 279 | WP_020801586 | helix-turn-helix transcriptional regulator | - |
| HTK76_RS00430 | 70469..70870 | + | 402 | WP_015065522 | type II toxin-antitoxin system HigA family antitoxin | - |
| HTK76_RS00435 | 71699..72520 | + | 822 | WP_004152492 | DUF932 domain-containing protein | - |
| HTK76_RS00440 | 72553..72882 | + | 330 | WP_047066474 | DUF5983 family protein | - |
| HTK76_RS00445 | 72915..73400 | - | 486 | WP_001568108 | transglycosylase SLT domain-containing protein | virB1 |
| HTK76_RS00450 | 73791..74207 | + | 417 | WP_072145360 | conjugal transfer relaxosome DNA-binding protein TraM | - |
| HTK76_RS00455 | 74407..75126 | + | 720 | WP_015065526 | hypothetical protein | - |
| HTK76_RS00460 | 75259..75462 | + | 204 | WP_171486139 | TraY domain-containing protein | - |
| HTK76_RS00465 | 75527..75895 | + | 369 | WP_004194426 | type IV conjugative transfer system pilin TraA | - |
| HTK76_RS00470 | 75909..76214 | + | 306 | WP_004144424 | type IV conjugative transfer system protein TraL | traL |
| HTK76_RS00475 | 76234..76800 | + | 567 | WP_004144423 | type IV conjugative transfer system protein TraE | traE |
| HTK76_RS00480 | 76787..77527 | + | 741 | WP_004152497 | type-F conjugative transfer system secretin TraK | traK |
| HTK76_RS00485 | 77527..78951 | + | 1425 | WP_015065626 | F-type conjugal transfer pilus assembly protein TraB | traB |
| HTK76_RS00490 | 79025..79609 | + | 585 | WP_020277945 | type IV conjugative transfer system lipoprotein TraV | traV |
| HTK76_RS00660 | 79764..80162 | + | 399 | WP_020277946 | hypothetical protein | - |
| HTK76_RS00500 | 80233..80481 | + | 249 | WP_223175793 | hypothetical protein | - |
| HTK76_RS00505 | 80505..80723 | + | 219 | WP_004195468 | hypothetical protein | - |
| HTK76_RS00510 | 80724..81035 | + | 312 | WP_047066479 | hypothetical protein | - |
| HTK76_RS00515 | 81102..81506 | + | 405 | WP_004197817 | hypothetical protein | - |
| HTK76_RS00520 | 81882..82280 | + | 399 | WP_023179972 | hypothetical protein | - |
| HTK76_RS00525 | 82352..84991 | + | 2640 | WP_032431388 | type IV secretion system protein TraC | virb4 |
| HTK76_RS00530 | 84991..85380 | + | 390 | WP_032736784 | type-F conjugative transfer system protein TrbI | - |
| HTK76_RS00535 | 85380..86006 | + | 627 | WP_020277949 | type-F conjugative transfer system protein TraW | traW |
| HTK76_RS00540 | 86048..86437 | + | 390 | WP_020277950 | hypothetical protein | - |
| HTK76_RS00545 | 86434..87423 | + | 990 | WP_009309872 | conjugal transfer pilus assembly protein TraU | traU |
| HTK76_RS00550 | 87436..88074 | + | 639 | WP_015065635 | type-F conjugative transfer system pilin assembly protein TrbC | trbC |
| HTK76_RS00555 | 88133..90088 | + | 1956 | WP_020277951 | type-F conjugative transfer system mating-pair stabilization protein TraN | traN |
| HTK76_RS00560 | 90120..90374 | + | 255 | WP_049194392 | conjugal transfer protein TrbE | - |
| HTK76_RS00565 | 90352..90600 | + | 249 | WP_004152675 | hypothetical protein | - |
| HTK76_RS00570 | 90613..90939 | + | 327 | WP_004144402 | hypothetical protein | - |
| HTK76_RS00575 | 90960..91712 | + | 753 | WP_004152677 | type-F conjugative transfer system pilin assembly protein TraF | traF |
| HTK76_RS00580 | 91723..91962 | + | 240 | WP_004144400 | type-F conjugative transfer system pilin chaperone TraQ | - |
| HTK76_RS00585 | 91934..92491 | + | 558 | WP_032736782 | type-F conjugative transfer system pilin assembly thiol-disulfide isomerase TrbB | traF |
| HTK76_RS00590 | 92537..92980 | + | 444 | WP_020277953 | F-type conjugal transfer protein TrbF | - |
| HTK76_RS00595 | 92958..94337 | + | 1380 | WP_014343487 | conjugal transfer pilus assembly protein TraH | traH |
| HTK76_RS00600 | 94337..97186 | + | 2850 | WP_172689276 | conjugal transfer mating-pair stabilization protein TraG | traG |
| HTK76_RS00605 | 97189..97722 | + | 534 | WP_014343486 | conjugal transfer protein TraS | - |
| HTK76_RS00610 | 97908..98639 | + | 732 | WP_004152629 | conjugal transfer complement resistance protein TraT | - |
| HTK76_RS00615 | 98832..99521 | + | 690 | WP_014343485 | hypothetical protein | - |
| HTK76_RS00620 | 99648..101960 | + | 2313 | WP_020277955 | type IV conjugative transfer system coupling protein TraD | virb4 |
Host bacterium
| ID | 19936 | GenBank | NZ_KY093014 |
| Plasmid name | pEaer-4382s | Incompatibility group | IncFII |
| Plasmid size | 108772 bp | Coordinate of oriT [Strand] | 73473..73522 [-] |
| Host baterium | Klebsiella aerogenes strain Eaer-4382 |
Cargo genes
| Drug resistance gene | blaKPC-3 |
| Virulence gene | - |
| Metal resistance gene | - |
| Degradation gene | - |
| Symbiosis gene | - |
| Anti-CRISPR | AcrIE9 |