Detailed information of oriT

oriT

The information of the oriT region


oriTDB ID   119505
Name   oriT_pEaer-4382b in_silico
Organism   Klebsiella aerogenes strain Eaer-4382
Sequence Completeness      -
NCBI accession of oriT (coordinates [strand])   NZ_KY093013 (99222..99271 [-], 50 nt)
oriT length   50 nt
IRs (inverted repeats)      7..14, 17..24  (GCAAAATT..AATTTTGC)
Location of nic site      33..34
Conserved sequence flanking the
  nic site    		  TGTGTGGTGA
Note   Predicted by oriTfinder 2.0

  oriT sequence  


Download         Length: 50 nt

>oriT_pEaer-4382b
AAATCTGCAAAATTTTAATTTTGCGTAGTGTGTGGTGATTTTGTGGTGAG

Visualization of oriT structure

  oriT secondary structure

Predicted by RNAfold.

Download structure file


Relaxase

ID   12321 GenBank   WP_116290300
Name   traI_HTK88_RS00750_pEaer-4382b insolico UniProt ID   _
Length   1752 a.a. PDB ID   
Note   Predicted by oriTfinder 2.0

  Relaxase protein sequence


Download         Length: 1752 a.a.        Molecular weight: 190657.44 Da        Isoelectric Point: 6.1039

>WP_116290300.1 MULTISPECIES: conjugative transfer relaxase/helicase TraI [Klebsiella]
MLSFSQVKSAGSAGNYYTEKDNYYVIGSMEERWQGKGAELLGLEGKVDKQVFTELLQGKLPDGSDLTRIQ
DGVNKHRPGYDLTFSAPKSVSMLAMLGGDKRLIDAHNRAVTVALNQVESLASTRVKKDGVSETVLTGNLI
IARFNHDTSRAQDPQIHTHSVVINATQNGDKWLTLASDTVGKTGFSETILANRIAFGKIYQNSLRADVES
MGYKTVDAGRNGMWEMEGVPVESFSTRSQELREAAGPDASLKSRDVAALDTRKSKEAIDPAEKMVEWMNT
LKETGFDIRGYREAADARAAELARAPAAPVNTDGPDITDVVTKAIAGLSDRKVQFTYADLLARTVGQLEA
KDGVFELARKGINAAIEREQLIPLDREKGLFTSNIHVLDELAVKALSQEVQRQNHVSVTPDASVVRQVPF
SDAVSVLAQDRPVMGIVSGQGGATGQRERVAELTLMAREQGRDVHILAADNRSRDFLAGDVRLAGETVTG
KSALQDGTAFIPGGTLIVDQAEKLSLKETISLLDGAMRHNVQVLLSDSGKRSGTGSALTVLKDSGVNTYR
WQGGHQTTADIISEPDKGARYSRLAQEFAVSVREGQESVAQISGTREQSVLNGLIRDSLRQEGVLGEKDT
TITALTPVWLDSKSRGVRDYYREGMVMERWDPETRTHDRFVIDRVTASSNMLTLKDREGDRLDLKVSAVD
SQWTLFRADTLPVAEGERLAVLGKIPDTRLKGGESVTVMKVEDGQLTVQRPGQKTTQTLAVGAGVFDGIK
VGHGWVESPGRSVSETATVFASVTQRELDNATLNQLAQSGSHLRLYSAQDAARTTEKLSRHTAFSVVSEQ
LKSRSGETDLDAAIAQQKAGLHTPAEQAIHLAIPLLESQDLTFSRPQLLATAMETGGGKVSMADIDTTIQ
TQIRSGQLLNVPVAHGYGNDLLISRQTWDAEKSILTHVLEGKDAVAPLMDRVPASLMTDLTAGQRAATRM
ILESTDRFTVVQGYAGVGKTTQFRAVMSAISLLPEETRPRVIGLAPTHRAVGEMQSAGVEARTTASFLHD
TQLLQRNGQTPDFSNTLFLLDESSMVGLADMAKAHSLIVAGGGRAVSSGDNDQLQPIAPGQPFRLMQQRS
AADVAIMKEIVRQMPELRPAVYSLIERDVHRALTTIEQVTPEQVPRKEGSWAPGSSVVEFTPKQEKAIEK
ALSEGKTLPEGQPATLYEALVKDYTGRTPEAQSQTLVITHLNKDRRALNSLIHDARRENGETGKEEITLP
VLVTSNIRDGELRKLSTWTAHKEAVALVDNVYHRISKVDKDNQLITLTDSEGKERFISPREASAEGVTLY
RQEKITVSQGDRMRFSKSDPERGYVANSIWEVQSVSGDSVTLSDGKITRTLTPKAEQAQQHIDLAYAITA
HGAQGASEPYAIALEGVAGGREQMASFESAYVALSRMKQHVQVYTDNREGWIKAIKNSPEKATAHDILEP
RNDRAVKTADLLFGWARPLDETAAGRAALQQSGLAQGSSPGKFISPGKKYPQPHVALPAFDKNGKAAGIW
LSPLTDRDGRLEAIGGEGRIMGNEDARFVALQNSRNGESLLAGNMGEGVRMARDNPDTGVVVRLAGDDRP
WNPGAMTGGRVWAEPAPVAPVPQAGADIILPPEVLAQRAAEEQQRREMEKQAEQTAREVAGEARKAGEPA
DRVKEVIGDVIRGLERDRPGTEKTTLPDDPQFRRQEAAIQQVASERLQRERLQAVERDMVRDLNREKTLG
GD

  Protein domains


Predicted by InterproScan.

DNA helicase TraI (TraI_C)

(1432-1554)

single-stranded DNA binding TraI N-terminal subdomain (ssDNA_TraI_N)

(576-628)

AAA domain (AAA_30)

(970-1156)

TrwC relaxase (TrwC)

(11-283)

DNA relaxase TraI 2B/2B-like domain (TraI_2B)

(634-713)


  Protein structure



No available structure.




T4CP

ID   14469 GenBank   WP_020277955
Name   traD_HTK88_RS00745_pEaer-4382b insolico UniProt ID   _
Length   770 a.a. PDB ID   _
Note   Predicted by oriTfinder 2.0

  T4CP protein sequence


Download         Length: 770 a.a.        Molecular weight: 85894.89 Da        Isoelectric Point: 5.1129

>WP_020277955.1 MULTISPECIES: type IV conjugative transfer system coupling protein TraD [Enterobacteriaceae]
MSFNAKDMTQGGQIANMRFRMFGQIANIIFYVLFILFWVLCGLMLMYRLSWQTFVNGCVYWWCTTLGPMR
DIIRSQPVYTIQYYGQSLEYTSEQILADKYTIWCGEQLWTSFVFAAVVSLVICIVTFFIASWVLGRQGKQ
QSEDENTGGRQLSDKPKEVARQMKRDGMASDIKIGDLPILKNSEIQNFCLHGTVGSGKSEVIRRLLNYVR
ARGDMAIIYDRSCEFVKSYYDPSLDKILNPLDSRCAAWDLWKECLTLPDFDNISNTLIPMGTKEDPFWQG
SGRTIFAEGAYLMREDKDRSYEKLVDTMLSIKIDKLRAYLQNTPAANLVEEKIEKTAISIRAVLTNYVKA
IRYLQGIEKNGEPFTIRDWMRGVREDRPNGWLFISSNADTHASLKPVISMWLSIAIRGLLAMGENRNRRV
WIFADELPTLHKLPDLVEILPEARKFGGCYVFGIQSYAQLEDIYGVKPAATLFDVMNTRAFFRSPSKEIA
EFAAGEIGEKEILKASEQYSYGADPVRDGVSTGKEKERETLVSYSDIQTLPDLSCYVTLPGPYPAVKLAL
KYKPRPKIAEGFIPRTLDARVDARLSALLEAREAEGSLARALFTPDAPASGPADTNSHAGEQPEPVSQPA
PADMTVSPEPVKAPPTTKSPAAEPSVRTTEPSVLRVTTVPLIKPKAAAAAAAASTASSSGAPATAAGGTQ
QELAQQSAEQGQDMLPAGMNEDGVIEDMQAYDAWLADEQTQRDMQRREEVNINHSHRHDEQDDVEIGGNI

  Protein domains


Predicted by InterproScan.

Type IV secretion-system coupling protein DNA-binding domain (TrwB_AAD_bind)

(172-560)

F sex factor protein N terminal (TraD_N)

(32-128)

  Protein structure



No available structure.




T4SS

T4SS were predicted by using oriTfinder2.

Region 1: 98664..127709

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HTK88_RS00535 94684..95040 + 357 WP_019706019 hypothetical protein -
HTK88_RS00540 95101..95313 + 213 WP_019706020 hypothetical protein -
HTK88_RS00545 95324..95548 + 225 WP_014343499 hypothetical protein -
HTK88_RS00550 95629..95949 + 321 WP_004152720 type II toxin-antitoxin system RelE/ParE family toxin -
HTK88_RS00555 95939..96217 + 279 WP_020801586 helix-turn-helix transcriptional regulator -
HTK88_RS00560 96218..96619 + 402 WP_015065522 type II toxin-antitoxin system HigA family antitoxin -
HTK88_RS00565 97448..98269 + 822 WP_004152492 DUF932 domain-containing protein -
HTK88_RS00570 98302..98631 + 330 WP_047066474 DUF5983 family protein -
HTK88_RS00575 98664..99149 - 486 WP_001568108 transglycosylase SLT domain-containing protein virB1
HTK88_RS00580 99540..99956 + 417 WP_072145360 conjugal transfer relaxosome DNA-binding protein TraM -
HTK88_RS00585 100156..100875 + 720 WP_015065526 hypothetical protein -
HTK88_RS00590 101008..101211 + 204 WP_171486139 TraY domain-containing protein -
HTK88_RS00595 101276..101644 + 369 WP_004194426 type IV conjugative transfer system pilin TraA -
HTK88_RS00600 101658..101963 + 306 WP_004144424 type IV conjugative transfer system protein TraL traL
HTK88_RS00605 101983..102549 + 567 WP_004144423 type IV conjugative transfer system protein TraE traE
HTK88_RS00610 102536..103276 + 741 WP_004152497 type-F conjugative transfer system secretin TraK traK
HTK88_RS00615 103276..104700 + 1425 WP_015065626 F-type conjugal transfer pilus assembly protein TraB traB
HTK88_RS00620 104774..105358 + 585 WP_020277945 type IV conjugative transfer system lipoprotein TraV traV
HTK88_RS00785 105513..105911 + 399 WP_020277946 hypothetical protein -
HTK88_RS00790 105982..106230 + 249 WP_223175793 hypothetical protein -
HTK88_RS00630 106254..106472 + 219 WP_004195468 hypothetical protein -
HTK88_RS00635 106473..106784 + 312 WP_047066479 hypothetical protein -
HTK88_RS00640 106851..107255 + 405 WP_004197817 hypothetical protein -
HTK88_RS00645 107631..108029 + 399 WP_023179972 hypothetical protein -
HTK88_RS00650 108101..110740 + 2640 WP_032431388 type IV secretion system protein TraC virb4
HTK88_RS00655 110740..111129 + 390 WP_032736784 type-F conjugative transfer system protein TrbI -
HTK88_RS00660 111129..111755 + 627 WP_020277949 type-F conjugative transfer system protein TraW traW
HTK88_RS00665 111797..112186 + 390 WP_020277950 hypothetical protein -
HTK88_RS00670 112183..113172 + 990 WP_009309872 conjugal transfer pilus assembly protein TraU traU
HTK88_RS00675 113185..113823 + 639 WP_015065635 type-F conjugative transfer system pilin assembly protein TrbC trbC
HTK88_RS00680 113882..115837 + 1956 WP_020277951 type-F conjugative transfer system mating-pair stabilization protein TraN traN
HTK88_RS00685 115869..116123 + 255 WP_049194392 conjugal transfer protein TrbE -
HTK88_RS00690 116101..116349 + 249 WP_004152675 hypothetical protein -
HTK88_RS00695 116362..116688 + 327 WP_004144402 hypothetical protein -
HTK88_RS00700 116709..117461 + 753 WP_004152677 type-F conjugative transfer system pilin assembly protein TraF traF
HTK88_RS00705 117472..117711 + 240 WP_004144400 type-F conjugative transfer system pilin chaperone TraQ -
HTK88_RS00710 117683..118240 + 558 WP_032736782 type-F conjugative transfer system pilin assembly thiol-disulfide isomerase TrbB traF
HTK88_RS00715 118286..118729 + 444 WP_020277953 F-type conjugal transfer protein TrbF -
HTK88_RS00720 118707..120086 + 1380 WP_014343487 conjugal transfer pilus assembly protein TraH traH
HTK88_RS00725 120086..122935 + 2850 WP_172689276 conjugal transfer mating-pair stabilization protein TraG traG
HTK88_RS00730 122938..123471 + 534 WP_014343486 conjugal transfer protein TraS -
HTK88_RS00735 123657..124388 + 732 WP_004152629 conjugal transfer complement resistance protein TraT -
HTK88_RS00740 124581..125270 + 690 WP_014343485 hypothetical protein -
HTK88_RS00745 125397..127709 + 2313 WP_020277955 type IV conjugative transfer system coupling protein TraD virb4

Host bacterium

ID   19935 GenBank   NZ_KY093013
Plasmid name   pEaer-4382b Incompatibility group   IncFII
Plasmid size   134521 bp Coordinate of oriT [Strand]   99222..99271 [-]
Host baterium   Klebsiella aerogenes strain Eaer-4382

Cargo genes

Drug resistance gene   ant(2'')-Ia, aadA6, blaOXA-2, formA, blaKPC-3
Virulence gene   -
Metal resistance gene   -
Degradation gene   -
Symbiosis gene   -
Anti-CRISPR   AcrIE9