Detailed information of oriT
oriT
The information of the oriT region
oriTDB ID | 119378 |
Name | oriT_p34AB |
Organism | Acinetobacter baumannii strain 34AB |
Sequence Completeness | - |
NCBI accession of oriT (coordinates [strand]) | NZ_MK134375 (24154..24257 [+], 104 nt) |
oriT length | 104 nt |
IRs (inverted repeats) | 63..70, 73..80 (CACCATGC..GCATGGTG) |
Location of nic site | _ |
Conserved sequence flanking the nic site |
_ |
Note | Predicted by oriTfinder 2.0 |
oriT sequence
Download Length: 104 nt
>oriT_p34AB
GCCCCGCAGGGCAGGATTCCCGTTGAGCGCCGCAGGTGCGAATAAGGGGAAGTGAAGAGGAACACCATGCTTGCATGGTGGGCCTACTTCACACATCCTGCCCG
GCCCCGCAGGGCAGGATTCCCGTTGAGCGCCGCAGGTGCGAATAAGGGGAAGTGAAGAGGAACACCATGCTTGCATGGTGGGCCTACTTCACACATCCTGCCCG
Visualization of oriT structure
oriT secondary structure
Predicted by RNAfold.
Download structure fileT4CP
ID | 14369 | GenBank | WP_042892356 |
Name | t4cp2_HPG65_RS01325_p34AB | UniProt ID | _ |
Length | 930 a.a. | PDB ID | _ |
Note | Predicted by oriTfinder 2.0 |
T4CP protein sequence
Download Length: 930 a.a. Molecular weight: 104210.92 Da Isoelectric Point: 6.1065
>WP_042892356.1 MULTISPECIES: type IV secretion protein DotL [Acinetobacter]
MASTQKYGTQTKFRPSSLKRDTRSGLEKIHLKLTAPDGANYLVGVLGAIAVILFVVPFGGEIFIGIAYYL
LKRYTHPNDYFFEWPFRAPLHSNSLDASTDITSRVKDDVLDNPKAFLEYARTNRDKLQGEGVTYLGRCRE
TGLPVYASNSDDRTHQIVLGTTGSGKTEYMLGNCANQYVQNSGYIFVDAKGDTKAQQDHYRLCNRFGRNE
DLLTINFITSGRNMLRAQTDKMTNSMNQMSNTSSGMLIEFLINLLDDSGGGGGDMWKGRAMAFIAALTRV
LVYLRDNGFIQLSPKVFTQYMELEALEELVFCHNDKYGIDFERVAEQLQGYLVSLPGYSSNPKKLKKQET
KTREQHGYITMQLTKAINDLTFNYGHIFGVEHGGDIDIFDVVLNRRILTVPLPALERSPDSLKMLGKLII
GSIKQMMAGSLGNKMEGLRRAIIDARPTNATTSFKLFLDEWGYIVIVGASVLPAQARSLNFSITFGAQTF
EDIERGSKEEAAATWGNTTVKAIGRTTSGAESTTYKLVDGFAGEEWQGKVNSINMYQGLVFNRSVPQTEV
QFAKEKRVTIEQIAGQHNGEFTLLISTKGEGGKTSDVKIVSMLAFYVAGDQPKYLRLNDLCPIFNIQKSE
IYDPTLKIEDFIHEIEEKHTLLTDNNSSANVTALGDFEICRKLNNTLYENNNTVKDFTQDFVHQILEARR
LESITPEGMAPGQRTVMSSSTAKGEISKEQKHRESIVSAQKEANIKDQIIEKRKQFQYFGKDSDISPQVQ
AGAYNNADLDAEFNIYGERISEPEQAQQESDVETVTRAVITPMNLDSYYDELKEILTGYDLTVAPITEYQ
LVQIPRHVKPEDQTQYIAENKASLDQAISHSTKNEMEFVEQQYAAKFEKALMKNPFSHECVTFEKLLVIL
GSKGSSASLRSGITIKKKDD
MASTQKYGTQTKFRPSSLKRDTRSGLEKIHLKLTAPDGANYLVGVLGAIAVILFVVPFGGEIFIGIAYYL
LKRYTHPNDYFFEWPFRAPLHSNSLDASTDITSRVKDDVLDNPKAFLEYARTNRDKLQGEGVTYLGRCRE
TGLPVYASNSDDRTHQIVLGTTGSGKTEYMLGNCANQYVQNSGYIFVDAKGDTKAQQDHYRLCNRFGRNE
DLLTINFITSGRNMLRAQTDKMTNSMNQMSNTSSGMLIEFLINLLDDSGGGGGDMWKGRAMAFIAALTRV
LVYLRDNGFIQLSPKVFTQYMELEALEELVFCHNDKYGIDFERVAEQLQGYLVSLPGYSSNPKKLKKQET
KTREQHGYITMQLTKAINDLTFNYGHIFGVEHGGDIDIFDVVLNRRILTVPLPALERSPDSLKMLGKLII
GSIKQMMAGSLGNKMEGLRRAIIDARPTNATTSFKLFLDEWGYIVIVGASVLPAQARSLNFSITFGAQTF
EDIERGSKEEAAATWGNTTVKAIGRTTSGAESTTYKLVDGFAGEEWQGKVNSINMYQGLVFNRSVPQTEV
QFAKEKRVTIEQIAGQHNGEFTLLISTKGEGGKTSDVKIVSMLAFYVAGDQPKYLRLNDLCPIFNIQKSE
IYDPTLKIEDFIHEIEEKHTLLTDNNSSANVTALGDFEICRKLNNTLYENNNTVKDFTQDFVHQILEARR
LESITPEGMAPGQRTVMSSSTAKGEISKEQKHRESIVSAQKEANIKDQIIEKRKQFQYFGKDSDISPQVQ
AGAYNNADLDAEFNIYGERISEPEQAQQESDVETVTRAVITPMNLDSYYDELKEILTGYDLTVAPITEYQ
LVQIPRHVKPEDQTQYIAENKASLDQAISHSTKNEMEFVEQQYAAKFEKALMKNPFSHECVTFEKLLVIL
GSKGSSASLRSGITIKKKDD
Protein domains
No domain identified.
Protein structure
No available structure.
T4SS
T4SS were predicted by using oriTfinder2.
Region 1: 236189..270876
Locus tag | Coordinates | Strand | Size (bp) | Protein ID | Product | Description |
---|---|---|---|---|---|---|
HPG65_RS01140 | 231297..232538 | + | 1242 | WP_004826931 | group II intron reverse transcriptase/maturase | - |
HPG65_RS01145 | 232618..233103 | + | 486 | Protein_226 | IS3 family transposase | - |
HPG65_RS01150 | 233153..235009 | + | 1857 | WP_159123820 | hypothetical protein | - |
HPG65_RS01155 | 235058..235459 | - | 402 | WP_159123819 | hypothetical protein | - |
HPG65_RS01160 | 235509..235853 | - | 345 | WP_228719902 | hypothetical protein | - |
HPG65_RS01165 | 236189..239194 | + | 3006 | WP_005028667 | DotA/TraY family protein | traY |
HPG65_RS01170 | 239256..239750 | - | 495 | WP_005028665 | hypothetical protein | - |
HPG65_RS01175 | 239994..242006 | + | 2013 | WP_005028664 | peptidoglycan DD-metalloendopeptidase family protein | traW |
HPG65_RS01180 | 242029..242439 | + | 411 | WP_005005820 | hypothetical protein | - |
HPG65_RS01185 | 242454..242879 | + | 426 | WP_005028662 | hypothetical protein | - |
HPG65_RS01190 | 242932..243576 | - | 645 | WP_005028658 | hypothetical protein | - |
HPG65_RS01195 | 243569..245125 | - | 1557 | WP_171265769 | UvrD-helicase domain-containing protein | - |
HPG65_RS01200 | 245129..245686 | - | 558 | WP_005028652 | hypothetical protein | - |
HPG65_RS01205 | 245686..246255 | - | 570 | WP_005028650 | hypothetical protein | - |
HPG65_RS01210 | 246268..246942 | - | 675 | WP_005028648 | hypothetical protein | - |
HPG65_RS01215 | 246955..247860 | - | 906 | WP_005028646 | hypothetical protein | - |
HPG65_RS01400 | 247983..249512 | - | 1530 | WP_024160802 | Type IV secretion system protein IcmP/DotM | trbA |
HPG65_RS01230 | 250782..251618 | + | 837 | WP_005028640 | ParA family protein | - |
HPG65_RS01235 | 251628..252689 | + | 1062 | WP_024160785 | ParB/RepB/Spo0J family partition protein | - |
HPG65_RS01240 | 252735..253562 | - | 828 | WP_024160786 | hypothetical protein | - |
HPG65_RS01245 | 253559..254665 | - | 1107 | WP_024160787 | ParM/StbA family protein | - |
HPG65_RS01250 | 254692..254961 | - | 270 | WP_005005884 | hypothetical protein | - |
HPG65_RS01255 | 254971..256266 | - | 1296 | WP_024160788 | ATPase, T2SS/T4P/T4SS family | virB11 |
HPG65_RS01260 | 256294..257103 | - | 810 | WP_024160789 | type IV secretory system conjugative DNA transfer family protein | traI |
HPG65_RS01265 | 257141..257587 | - | 447 | WP_024160790 | DotD/TraH family lipoprotein | - |
HPG65_RS01270 | 258666..259895 | + | 1230 | WP_227555133 | hypothetical protein | - |
HPG65_RS01275 | 259896..260183 | + | 288 | WP_024160792 | hypothetical protein | - |
HPG65_RS01280 | 260194..260877 | + | 684 | WP_005005897 | DotI/IcmL family type IV secretion protein | traM |
HPG65_RS01285 | 260891..261838 | + | 948 | WP_005005900 | DotH/IcmK family type IV secretion protein | traN |
HPG65_RS01290 | 261860..263371 | + | 1512 | WP_024160793 | DotG/IcmE/VirB10 family protein | - |
HPG65_RS01295 | 263377..264258 | + | 882 | WP_024160794 | hypothetical protein | - |
HPG65_RS01300 | 264269..264844 | + | 576 | WP_024160795 | hypothetical protein | - |
HPG65_RS01305 | 265012..265425 | + | 414 | WP_024160796 | hypothetical protein | - |
HPG65_RS01310 | 265536..266423 | + | 888 | WP_024160797 | HNH endonuclease | - |
HPG65_RS01315 | 266434..267636 | + | 1203 | WP_024160951 | hypothetical protein | - |
HPG65_RS01320 | 267694..270876 | + | 3183 | WP_166135296 | hypothetical protein | traU |
HPG65_RS01325 | 270999..273791 | + | 2793 | WP_042892356 | type IV secretion protein DotL | - |
HPG65_RS01330 | 273832..274032 | - | 201 | WP_078389757 | hypothetical protein | - |
HPG65_RS01335 | 274427..274600 | + | 174 | WP_162900306 | hypothetical protein | - |
HPG65_RS01340 | 274687..275775 | + | 1089 | WP_171265770 | DUF6685 family protein | - |
Host bacterium
ID | 19808 | GenBank | NZ_MK134375 |
Plasmid name | p34AB | Incompatibility group | - |
Plasmid size | 277864 bp | Coordinate of oriT [Strand] | 24154..24257 [+] |
Host baterium | Acinetobacter baumannii strain 34AB |
Cargo genes
Drug resistance gene | tet(X3), sul2, aph(3')-VIa, aac(3)-IId, msr(E), mph(E), floR, tet(M) |
Virulence gene | - |
Metal resistance gene | - |
Degradation gene | - |
Symbiosis gene | - |
Anti-CRISPR | AcrVA2 |