Detailed information of oriT

oriT

The information of the oriT region


oriTDB ID   119247
Name   oriT_KPCTRSRTH05|unnamed in_silico
Organism   Klebsiella quasipneumoniae subsp. similipneumoniae strain KPCTRSRTH05
Sequence Completeness      -
NCBI accession of oriT (coordinates [strand])   NZ_CM016896 (47999..48080 [+], 82 nt)
oriT length   82 nt
IRs (inverted repeats)      27..32, 35..40  (GCCACC..GGTGGC)
Location of nic site      _
Conserved sequence flanking the
  nic site    		  _
Note   Predicted by oriTfinder 2.0

  oriT sequence  


Download         Length: 82 nt

>oriT_KPCTRSRTH05|unnamed
CGGGACAGGATATGTTTTGTATTACCGCCACCACGGTGGCAGCAATCCAAAACTCCTGGTCAGGGCGAAGCCCCGACACCCC

Visualization of oriT structure

  oriT secondary structure

Predicted by RNAfold.

Download structure file


Relaxase

ID   12176 GenBank   WP_032440569
Name   traI_CI663_RS00345_KPCTRSRTH05|unnamed insolico UniProt ID   A0A7G3L4G1
Length   642 a.a. PDB ID   
Note   Predicted by oriTfinder 2.0

  Relaxase protein sequence


Download         Length: 642 a.a.        Molecular weight: 74384.24 Da        Isoelectric Point: 8.3203

>WP_032440569.1 MULTISPECIES: TraI/MobA(P) family conjugative relaxase [Enterobacterales]
MNAIIPPKRRDRKSSFVQLVAYVSVRDDVPLKDELKEDERFQRPSRSRQAIFDRLVDYMNRSEGTIIEQI
SDMTPEGDIRCSVDGVICQHNLMSVETAAMEMNSIAMQNTHVKDAVYHYILSWQEDENPSDDQVFDSVKH
SLKRLGMEEHQYVAAIHRDTDNLHVHIAANRVHPVSYRAANVWNDADKLQRTCRELELKHGFKVDNGSWV
RDADNNIVRARKGYRNAPRSARQLEHFSDKESLHHFAVQHVRKPVNAMFREKNASWEKLHQVMNDAGLML
ETSGKGLIVRDVCGDEKLAVKSSRVHPGMTLSRLEPALGTFVPSPRSAEMVKQSVQTSYSDKLHVRDRGA
RAERRQARADARIDLRERYEKYRNAWKKPDLHAAERFRAVSAAFKEQKTHIRDNEKDAHMRKLMYHIVEF
EREKSMAALRIALKAERQKLYDEGMMRPLTYRAWTEQQALHGDQAALSQLRGWSYRQNRKNRTPQASDNI
IYCAPADDTSLLKADGYEARLHRDGAVVYSRQGQDAIIDRGDRVEVINPYDDGDLNTRIAVDMVAWKSGE
RVEFSRDGSFAYAAGDAVAAHNLHHRDSIIVPSDREQYDYTREKFNQLKGRYTDSEREALRQQYDDRYYD
DVRHNDDSSYKP

  Protein domains


Predicted by InterproScan.

Relaxase/Mobilisation nuclease domain (Relaxase)

(52-307)


  Protein structure


Source ID Structure
AlphaFold DB A0A7G3L4G1


T4CP

ID   14260 GenBank   WP_032440593
Name   trbC_CI663_RS00040_KPCTRSRTH05|unnamed insolico UniProt ID   _
Length   730 a.a. PDB ID   _
Note   Predicted by oriTfinder 2.0

  T4CP protein sequence


Download         Length: 730 a.a.        Molecular weight: 82679.78 Da        Isoelectric Point: 6.3201

>WP_032440593.1 MULTISPECIES: F-type conjugative transfer protein TrbC [Enterobacterales]
MQQKQKPVDAARLQRQVHQSMVEEMAYRTGPLLLAIIAIFGAAIVWPVLLWAAIPVMLVWVPMFMLNEFR
MPARMPMDMDRLDPSTETMRPGKLLGFLPVTKLNRHMEKAAGVFYTGYERTLDGGREIWLRMDDLTRHLI
LMATTGAGKTEMLLGFVLNALCWAKGLIFSDGKAQNDVWFAVASLSRRFGREDDLRLLNYITGGQSRSQR
LLENDKSRQQSNNTNPFALAQETYITQLMDSMMPEAGNDRTWQEKAKGMNQVLTKALIYKSRKEQKVMSQ
RLIQEYLSLDKFAELYLQAEAEEWHEEIRTGLRNYLSTSVPGFDMSLITKPSEWSQEARNQHGYLTSQYN
RMLSLFSDTYGHVFSSGAGDIDLRDVVHNDRILVILIPALELSASEALTLGRLTTSQLAMILSQDLGERM
EGKAEDILVIKKFRDRFPFLWLADEVGSYYSDTIGQLATQVRSLGYALVLAGQDLQKLKTAVGDKLWTLL
GNMFTRIFGTIIDPTETAEFAAKSAGMEYQAIQDSMEYRAGAIGGSYADTGRVTVQEKPKLPFDELQALN
QGEQATVFKGAVIRNNALYIADDDKITDKDVRINRFVEVEWPTYDSLSHLLPAKVRRDRPRPASINRIMA
IVKNDKIRHAPDSMIIQDGVLYAIADEAMYFDEVAPTPPNTVQRATQLLSLAAAVLTETRGRYRVEYPEP
EKLSVKKDELEKYQHIHQSAVTQQADGFSY

  Protein domains


Predicted by InterproScan.

TraM recognition site of TraD and TraG (TraG-D_C)

(439-560)

  Protein structure



No available structure.




T4SS

T4SS were predicted by using oriTfinder2.

Region 1: 57189..68676

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
CI663_RS00355 (CI663_026655) 52336..52830 - 495 WP_032440571 molybdopterin-dependent oxidoreductase -
CI663_RS00360 (CI663_026660) 52823..53752 - 930 WP_032440581 glycosyltransferase family 9 protein -
CI663_RS00365 (CI663_026665) 53812..54654 - 843 WP_032440572 helix-turn-helix domain-containing protein -
CI663_RS00370 (CI663_026670) 55264..55491 + 228 WP_032440573 hypothetical protein -
CI663_RS00375 (CI663_026675) 56223..56702 + 480 WP_032440574 lytic transglycosylase domain-containing protein -
CI663_RS00380 (CI663_026680) 56728..57192 + 465 WP_225546581 DotD/TraH family lipoprotein -
CI663_RS00385 (CI663_026685) 57189..58007 + 819 WP_032440576 type IV secretory system conjugative DNA transfer family protein traI
CI663_RS00390 (CI663_026690) 57985..59172 + 1188 WP_077255878 plasmid transfer ATPase TraJ virB11
CI663_RS00395 (CI663_026695) 59169..59435 + 267 WP_032440577 IcmT/TraK family protein traK
CI663_RS27370 59453..62758 + 3306 WP_058674281 LPD7 domain-containing protein -
CI663_RS00405 (CI663_026705) 62792..63145 + 354 WP_032440584 hypothetical protein traL
CI663_RS00410 (CI663_026710) 63217..63885 + 669 WP_032440585 DotI/IcmL/TraM family protein traM
CI663_RS00415 (CI663_026715) 63896..64999 + 1104 WP_077264204 DotH/IcmK family type IV secretion protein traN
CI663_RS00420 (CI663_026720) 65003..66343 + 1341 WP_032440586 conjugal transfer protein TraO traO
CI663_RS00425 (CI663_026725) 66350..67069 + 720 WP_050597333 conjugal transfer protein TraP traP
CI663_RS00430 (CI663_026730) 67079..67612 + 534 WP_032440587 conjugal transfer protein TraQ traQ
CI663_RS00435 (CI663_026735) 67668..68069 + 402 WP_032440588 DUF6750 family protein traR
CI663_RS00440 (CI663_026740) 68113..68676 + 564 WP_045325070 hypothetical protein traT

Host bacterium

ID   19677 GenBank   NZ_CM016896
Plasmid name   KPCTRSRTH05|unnamed Incompatibility group   IncFII
Plasmid size   71655 bp Coordinate of oriT [Strand]   47999..48080 [+]
Host baterium   Klebsiella quasipneumoniae subsp. similipneumoniae strain KPCTRSRTH05

Cargo genes

Drug resistance gene   mcr-3.22
Virulence gene   -
Metal resistance gene   -
Degradation gene   -
Symbiosis gene   -
Anti-CRISPR   -