Detailed information of oriT

oriT

The information of the oriT region

• oriTDB ID: 119224
• Name: oriT_pRMLUG2_2
• Organism: Staphylococcus lugdunensis strain RMLUG2
• Sequence Completeness: -
• NCBI accession of oriT (coordinates [strand]): NZ_CP084436 (6004..6123 [+], 120 nt)
• oriT length: 120 nt
• IRs (inverted repeats): 52..59, 61..68 (TTGGGGAT..ATCCCCAA) 27..32, 41..46 (TTATGT..ACATAA)
• Location of nic site: _
• Conserved sequence flanking the nic site: _
• Note: Predicted by oriTfinder 2.0

oriT sequence

• Length: 120 nt

>oriT_pRMLUG2_2
TGGCTAGCATTTAAATCACTCATTTTTTATGTAAGAAGAAACATAAGGGGCTTGGGGATTATCCCCAACAAGCAGGCGACACTACCACGTAAGTGGCTAGCAAAGCCAATGCTTGCCAAA

oriT secondary structure

Predicted by RNAfold.

Relaxase

Relaxase_LE165_RS12705_pRMLUG2_2

• ID: 12164
• GenBank: WP_000246206
• Name: Relaxase_LE165_RS12705_pRMLUG2_2
• UniProt ID: D2JGE5
• Length: 336 a.a.
• Note: Predicted by oriTfinder 2.0

Relaxase protein sequence

• Length: 336 a.a.
• Molecular weight: 38461.30 Da
• Isoelectric Point: 7.3199

>WP_000246206.1 MULTISPECIES: relaxase/mobilization nuclease domain-containing protein [Bacilli]
MATTKLSATKSTSRAINYAEKRAVEKSGLNCDVDYAKSSFKASRELYGKTDGNQGHVIIQSFKPGEVTPE
QCNQLGLALAEKLVPNHQVAVYTHNDTDHVHNHIVINSIDLETGKKFNNNKQALRNVRNFNDEVCMEHGL
SVPEKNTARLRYTQTEKAIADPNTKSTAQYSWKDEIREAIDQSQATNMDEFKDHLNQHGIEIERVTPKSI
TYRHLAEDKKVRGRKLGEDYNKGGIEDGFERQIQHQQQTERVSDTDVQPKRRTSSRDESTERDTGVTQAD
WDQFAQDTNELERSRQAAESARLADEKARRDREKRERAKQTSRRIINNDRDHGLEL

Protein domains

Predicted by InterproScan.

• Relaxase/Mobilisation nuclease domain (Relaxase) (9-242)

Protein structure

• AlphaFold DB: D2JGE5

Host bacterium

• ID: 19654
• GenBank: NZ_CP084436
• Plasmid name: pRMLUG2_2
• Incompatibility group: -
• Plasmid size: 22868 bp
• Coordinate of oriT [Strand]: 6004..6123 [+]
• Host baterium: Staphylococcus lugdunensis strain RMLUG2

Cargo genes

• Drug resistance gene: -
• Virulence gene: -
• Metal resistance gene: -
• Degradation gene: -
• Symbiosis gene: -
• Anti-CRISPR: -