Detailed information of oriT

oriT

The information of the oriT region


oriTDB ID   119169
Name   oriT_FDAARGOS_64|unnamed1 in_silico
Organism   Raoultella planticola strain FDAARGOS_64
Sequence Completeness      -
NCBI accession of oriT (coordinates [strand])   NZ_CP026048 (111794..111843 [+], 50 nt)
oriT length   50 nt
IRs (inverted repeats)      7..14, 17..24  (GCAAAATT..AATTTTGC)
Location of nic site      33..34
Conserved sequence flanking the
  nic site    		  TGTGTGGTGA
Note   Predicted by oriTfinder 2.0

  oriT sequence  


Download         Length: 50 nt

>oriT_FDAARGOS_64|unnamed1
AAATCTGCAAAATTTTAATTTTGCGTAGTGTGTGGTGATTTTGTGGTGAG

Visualization of oriT structure

  oriT secondary structure

Predicted by RNAfold.

Download structure file


Relaxase

ID   12123 GenBank   WP_047665716
Name   traI_MC50_RS26940_FDAARGOS_64|unnamed1 insolico UniProt ID   _
Length   1752 a.a. PDB ID   
Note   Predicted by oriTfinder 2.0

  Relaxase protein sequence


Download         Length: 1752 a.a.        Molecular weight: 190627.41 Da        Isoelectric Point: 6.1560

>WP_047665716.1 MULTISPECIES: conjugative transfer relaxase/helicase TraI [Klebsiella/Raoultella group]
MLSFSQVKSAGSAGNYYTEKDNYYVIGSMEERWQGKGAELLGLEGKVDKQVFTELLQGKLPDGSDLTRIQ
DGVNKHRPGYDLTFSAPKSVSMLAMLGGDKRLIDAHNRAVTVALNQVESLASTRVKKDGVSETVLTGNLI
IARFNHDTSRAQDPQIHTHSVVINATQNGDKWLTLASDTVGKTGFSETILANRIAFGKIYQNSLRADVES
MGYKTVDAGRNGMWEMEGVPVESFSTRSQELREAAGPDASLKSRDVAALDTRKSKEAIDPAEKMVEWMNT
LKETGFDIRGYREAADARAAELARAPAAPVNTDGPDITDVVTKAIAGLSDRKVQFTYADLLARTVGQLEA
KDGVFELARKGINAAIEREQLIPLDREKGLFTSNIHVLDELAVKALSQEVQRQNHVSVTPDASVVRQVPF
SDAVSVLAQDRPVMGIVSGQGGATGQRERVAELTLMAREQGRDVHILAADNRSRDFLAGDVRLAGETVTG
KSALQDGTAFIPGGTLIVDQAEKLSLKETISLLDGAMRHNVQVLLSDSGKRSGTGSALTVLKDSGVNTYR
WQGGHQTTADIISEPDKGARYSRLAQEFAVSVREGQESVAQISGTREQSVLNGLIRDSLRQEGVLGEKDT
TITALTPVWLDSKSRGVRDYYREGMVMERWDPETRTHDRFVIDRVTASSNMLTLKDREGDRLDLKVSAVD
SQWTLFRADTLPVAEGERLAVLGKIPDTRLKGGESVTVMKVEDGQLTVQRPGQKTTQTLAVGAGVFDGIK
VGHGWVESPGRSVSETATVFASVTQRELDNATLNQLAQSGSHLRLYSAQDAARTTEKLSRHTAFSVVSEQ
LKSRSGETDLDAAIAQQKAGLHTPAEQAIHLAIPLLESQDLTFSRPQLLATAMETGGGKVSMADIDTTIQ
TQIRSGQLLNVPVAHGYGNDLLISRQTWDAEKSILTHVLEGKDAVAPLMDRVPASLMTDLTAGQRAATRM
ILESTDRFTVVQGYAGVGKTTQFRAVMSAISLLPEETRPRVIGLAPTHRAVGEMQSAGVEARTTASFLHD
TQLLQRNGQTPDFSNTLFLLDESSMVGLADMAKAHSLIVAGGGRAVSSGDNDQLQPIAPGQPFRLMQQRS
AADVAIMKEIVRQMPELRPAVYSLIERDVHRALTTIEQVTPEQVPRKEGSWAPGSSVVEFTPKQEKAIEK
ALSEGKTLPEGQPATLYEALVKDYTGRTPEAQSQTLVITHLNKDRRALNSLIHDARRENGETGKEEITLP
VLVTSNIRDGELRKLSTWTAHKEAVALVDNVYHRISKVDKDNQLITLTDSEGKERFISPREASAEGVTLY
RQEKITVSQGDRMRFSKSDPERGYVANSIWEVQSVSGDSVTLSDGKITRTLTPKAEQAQQHIDLAYAITA
HGAQGASEPYAIALEGVAGGREQMASFESAYVALSRMKQHVQVYTDNREGWIKAIKNSPEKATAHDILEP
RNDRAVKTADLLFGRARPLDETAAGRAALQQSGLAQGSSPGKFISPGKKYPQPHVALPAFDKNGKAAGIW
LSPLTDRDGRLEAIGGEGRIMGNEDARFVALQNSRNGESLLAGNMGEGVRMARDNPDTGVVVRLAGDDRP
WNPGAMTGGRVWAEPAPVAPVPQAGADIILPPEVLAQRAAEEQQRREMEKQAEQTAREVAGEARKAGEPA
DRVKEVIGDVIRGLERDRPGTEKTTLPDDPQFRRQEAAIQQVASERLQRERLQAVERDMVRDLNREKTLG
GD

  Protein domains


Predicted by InterproScan.

DNA helicase TraI (TraI_C)

(1432-1554)

single-stranded DNA binding TraI N-terminal subdomain (ssDNA_TraI_N)

(576-628)

TrwC relaxase (TrwC)

(11-283)

DNA relaxase TraI 2B/2B-like domain (TraI_2B)

(634-713)

AAA domain (AAA_30)

(970-1156)


  Protein structure



No available structure.




T4SS

T4SS were predicted by using oriTfinder2.

Region 1: 88130..112401

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
MC50_RS26945 (MC50_026945) 83356..85668 - 2313 Protein_104 type IV conjugative transfer system coupling protein TraD -
MC50_RS28750 85795..86484 - 690 WP_014343485 hypothetical protein -
MC50_RS26955 (MC50_026955) 86677..87408 - 732 WP_004152629 conjugal transfer complement resistance protein TraT -
MC50_RS26960 (MC50_026960) 87594..88127 - 534 WP_080748465 conjugal transfer protein TraS -
MC50_RS26965 (MC50_026965) 88130..90979 - 2850 WP_020277954 conjugal transfer mating-pair stabilization protein TraG traG
MC50_RS26970 (MC50_026970) 90979..92358 - 1380 WP_014343487 conjugal transfer pilus assembly protein TraH traH
MC50_RS26975 (MC50_026975) 92336..92779 - 444 WP_020277953 F-type conjugal transfer protein TrbF -
MC50_RS26980 (MC50_026980) 92825..93382 - 558 WP_032736782 type-F conjugative transfer system pilin assembly thiol-disulfide isomerase TrbB traF
MC50_RS26985 (MC50_026985) 93354..93593 - 240 WP_004144400 type-F conjugative transfer system pilin chaperone TraQ -
MC50_RS26990 (MC50_026990) 93604..94356 - 753 WP_004152677 type-F conjugative transfer system pilin assembly protein TraF traF
MC50_RS26995 (MC50_026995) 94377..94703 - 327 WP_004144402 hypothetical protein -
MC50_RS27000 (MC50_027000) 94716..94964 - 249 WP_004152675 hypothetical protein -
MC50_RS27005 (MC50_027005) 94942..95196 - 255 WP_049194392 conjugal transfer protein TrbE -
MC50_RS27010 (MC50_027010) 95228..97183 - 1956 WP_020277951 type-F conjugative transfer system mating-pair stabilization protein TraN traN
MC50_RS27015 (MC50_027015) 97242..97880 - 639 WP_015065635 type-F conjugative transfer system pilin assembly protein TrbC trbC
MC50_RS27020 (MC50_027020) 97893..98882 - 990 WP_009309872 conjugal transfer pilus assembly protein TraU traU
MC50_RS27025 (MC50_027025) 98879..99268 - 390 WP_020277950 hypothetical protein -
MC50_RS27030 (MC50_027030) 99310..99936 - 627 WP_020277949 type-F conjugative transfer system protein TraW traW
MC50_RS27035 (MC50_027035) 99936..100325 - 390 WP_032736784 type-F conjugative transfer system protein TrbI -
MC50_RS27040 (MC50_027040) 100325..102964 - 2640 WP_032431388 type IV secretion system protein TraC virb4
MC50_RS27045 (MC50_027045) 103036..103434 - 399 WP_023179972 hypothetical protein -
MC50_RS27055 (MC50_027055) 103810..104214 - 405 WP_004197817 hypothetical protein -
MC50_RS27060 (MC50_027060) 104281..104592 - 312 WP_047066479 hypothetical protein -
MC50_RS27065 (MC50_027065) 104593..104811 - 219 WP_004195468 hypothetical protein -
MC50_RS28990 (MC50_027070) 104835..105083 - 249 WP_223175793 hypothetical protein -
MC50_RS28995 105154..105552 - 399 WP_020277946 hypothetical protein -
MC50_RS27080 (MC50_027080) 105707..106291 - 585 WP_020277945 type IV conjugative transfer system lipoprotein TraV traV
MC50_RS27085 (MC50_027085) 106365..107789 - 1425 WP_015065626 F-type conjugal transfer pilus assembly protein TraB traB
MC50_RS27090 (MC50_027090) 107789..108529 - 741 WP_004152497 type-F conjugative transfer system secretin TraK traK
MC50_RS27095 (MC50_027095) 108516..109082 - 567 WP_004144423 type IV conjugative transfer system protein TraE traE
MC50_RS27100 (MC50_027100) 109102..109407 - 306 WP_004144424 type IV conjugative transfer system protein TraL traL
MC50_RS27105 (MC50_027105) 109421..109789 - 369 WP_004194426 type IV conjugative transfer system pilin TraA -
MC50_RS27110 (MC50_027110) 109854..110057 - 204 WP_171486139 TraY domain-containing protein -
MC50_RS27115 (MC50_027115) 110190..110909 - 720 WP_015065526 hypothetical protein -
MC50_RS27120 (MC50_027120) 111109..111525 - 417 WP_072145360 conjugal transfer relaxosome DNA-binding protein TraM -
MC50_RS27125 (MC50_027125) 111916..112401 + 486 WP_001568108 transglycosylase SLT domain-containing protein virB1
MC50_RS27130 (MC50_027130) 112434..112763 - 330 WP_047066474 DUF5983 family protein -
MC50_RS27135 (MC50_027135) 112796..113617 - 822 WP_004152492 DUF932 domain-containing protein -
MC50_RS27155 (MC50_027155) 114446..114847 - 402 WP_015065522 type II toxin-antitoxin system HigA family antitoxin -
MC50_RS27160 (MC50_027160) 114848..115126 - 279 WP_020801586 helix-turn-helix transcriptional regulator -
MC50_RS27165 (MC50_027165) 115116..115436 - 321 WP_004152720 type II toxin-antitoxin system RelE/ParE family toxin -
MC50_RS27170 (MC50_027170) 115517..115741 - 225 WP_014343499 hypothetical protein -
MC50_RS27175 (MC50_027175) 115752..115964 - 213 WP_019706020 hypothetical protein -
MC50_RS27180 (MC50_027180) 116025..116381 - 357 WP_019706019 hypothetical protein -

Host bacterium

ID   19599 GenBank   NZ_CP026048
Plasmid name   FDAARGOS_64|unnamed1 Incompatibility group   IncFII
Plasmid size   127313 bp Coordinate of oriT [Strand]   111794..111843 [+]
Host baterium   Raoultella planticola strain FDAARGOS_64

Cargo genes

Drug resistance gene   -
Virulence gene   -
Metal resistance gene   -
Degradation gene   -
Symbiosis gene   -
Anti-CRISPR   AcrIE9