Detailed information of oriT
oriT
The information of the oriT region
| oriTDB ID | 119163 |
| Name | oriT_pKPN1705-3  |
| Organism | Klebsiella quasivariicola strain KPN1705 |
| Sequence Completeness | - |
| NCBI accession of oriT (coordinates [strand]) | NZ_CP022826 (44542..44646 [+], 105 nt) |
| oriT length | 105 nt |
| IRs (inverted repeats) | 32..37, 40..45 (GCCGGC..GCCGGC) |
| Location of nic site | _ |
| Conserved sequence flanking the nic site |
_ |
| Note | Predicted by oriTfinder 2.0 |
oriT sequence
Download Length: 105 nt
>oriT_pKPN1705-3
AGTACGGGACAAGATGTGTTTTTGGTACACCGCCGGCACGCCGGCAGTGACGCAAAAACGTCTTGGTCAGGGCAAGCCCCGACACCCCCTAACGAGGTTAGCTAT
AGTACGGGACAAGATGTGTTTTTGGTACACCGCCGGCACGCCGGCAGTGACGCAAAAACGTCTTGGTCAGGGCAAGCCCCGACACCCCCTAACGAGGTTAGCTAT
Visualization of oriT structure
oriT secondary structure
Predicted by RNAfold.
Download structure file
Relaxase
| ID | 12120 | GenBank | WP_004206924 |
| Name | traI_B8P98_RS30240_pKPN1705-3 |
UniProt ID | A0A2V4FJ40 |
| Length | 659 a.a. | PDB ID | |
| Note | Predicted by oriTfinder 2.0 | ||
Relaxase protein sequence
Download Length: 659 a.a. Molecular weight: 75087.84 Da Isoelectric Point: 10.0285
>WP_004206924.1 MULTISPECIES: TraI/MobA(P) family conjugative relaxase [Enterobacterales]
MVIPKIIEGRRDKKSSFGQLIKYMADKPSQELTDTVQPTPETALAVKSDMFEGLNNYLTRKQKVISTPVD
VEPGVQRVVVGDVTCQYNTFSLDGAAQEMNSVSQQSTRCKDPVMHYVLSWPDYEKPNDDQVFDSVKFTLA
SMGMSDHQYVAAIHRDTDNLHVHVAVNRINPQTYKAASSSFTKDTLHQACRLLELKNGWSHSNGAYVVND
RQQIVRNPHSKKERGNWRSLDRINKMENKEGVETLYRYIVGDEQVGGSRQNLIHVSAGLREAKSWDDVHK
TFAGIGLRVEKAQGKKGYVITHEHQNQKTAVKASLVFNKAQYTLKSMEERFGEYQPSHIEPAKVSVFKTA
YTPGAYRRDANKRLQRKIERAEERMLLKGRYRAYRNNLPIYSPDKDRIADEYRKIAQHTRLVKNNVRHSV
SDPHTRKLMYNLAEFKRLQAVANLRLSLREERNGFRAANPRLSYREWVEQEALKGDKAALSQMRGFAYSS
RKKEKYKQQLVEQIGFNRTFNAITSHDRDDVAVMASARHGVKPRLLKDGTVIFERDGKPVAADRGHIVLT
ESNGIDKEKTADLAIALTIAGKAKSVRVDGDGEFKELCCNRIVDAAVNHNHPVAQGITFTDAAQQAYAQN
EKHRLIREQNNSKNEMQLRSESDDKFNPK
MVIPKIIEGRRDKKSSFGQLIKYMADKPSQELTDTVQPTPETALAVKSDMFEGLNNYLTRKQKVISTPVD
VEPGVQRVVVGDVTCQYNTFSLDGAAQEMNSVSQQSTRCKDPVMHYVLSWPDYEKPNDDQVFDSVKFTLA
SMGMSDHQYVAAIHRDTDNLHVHVAVNRINPQTYKAASSSFTKDTLHQACRLLELKNGWSHSNGAYVVND
RQQIVRNPHSKKERGNWRSLDRINKMENKEGVETLYRYIVGDEQVGGSRQNLIHVSAGLREAKSWDDVHK
TFAGIGLRVEKAQGKKGYVITHEHQNQKTAVKASLVFNKAQYTLKSMEERFGEYQPSHIEPAKVSVFKTA
YTPGAYRRDANKRLQRKIERAEERMLLKGRYRAYRNNLPIYSPDKDRIADEYRKIAQHTRLVKNNVRHSV
SDPHTRKLMYNLAEFKRLQAVANLRLSLREERNGFRAANPRLSYREWVEQEALKGDKAALSQMRGFAYSS
RKKEKYKQQLVEQIGFNRTFNAITSHDRDDVAVMASARHGVKPRLLKDGTVIFERDGKPVAADRGHIVLT
ESNGIDKEKTADLAIALTIAGKAKSVRVDGDGEFKELCCNRIVDAAVNHNHPVAQGITFTDAAQQAYAQN
EKHRLIREQNNSKNEMQLRSESDDKFNPK
Protein domains
Predicted by InterproScan.
Protein structure
| Source | ID | Structure |
|---|---|---|
| AlphaFold DB | A0A2V4FJ40 |
T4CP
| ID | 14197 | GenBank | WP_004206885 |
| Name | t4cp2_B8P98_RS29995_pKPN1705-3 |
UniProt ID | _ |
| Length | 695 a.a. | PDB ID | _ |
| Note | Predicted by oriTfinder 2.0 | ||
T4CP protein sequence
Download Length: 695 a.a. Molecular weight: 79538.91 Da Isoelectric Point: 4.8317
>WP_004206885.1 MULTISPECIES: conjugal transfer protein TrbC [Enterobacterales]
MQQESVDSKKVLRPDGSFMEWFLTPNVQFGLLAILTVLGLFLPFTMLLSILILPPLMVAFTDRKFRPPLR
MPKDCEMFDDTLTTETQAEYRLGPIRIPHKVRERKKAKGILYVGYERGRLFGRELWLNMTDLLRHMVFFG
TTGSGKTETFYGFIVNFLLWCRGYCLSDGKADNKLAFATWSLARRFGREDDYYVLNLLTGSIDRFVNLVK
QESIPAQSNSVNLFSVAPPTFIIQLMESMLPQVGGDSAQWQDTAKAMMSALINALCYKRARGELLLSQRT
IQKNMSLPAMAALYVEAKKNGWHSEGYAALESYLENTPGFLLANAEYPETWEARAFEQHNYMSRQFLKTL
SLFNETYGHVFPEDSGDIRMDDIFHNDRILIVMIPSLELSRGEAATLGRLYVTLQRMTISKDLGYQLEGK
KEEVLLTHALNNQAPYGLIYDELGQYFTSGMDTLSAQMRSLEKMGVFSSQDHPSLARGANGEVDSLIANT
RVKYFESIEDRKTFEILRETVGQDYYSELSGQEAEHGTFSKTVREGDLYQIREKDRVNIRELRKQTEGQG
VISFQDALVRSAAFYIPDNEKFSSELPMRINRFIEVLPPSPATLYSIYPERKDDELAETNPDAMRFPPIK
LFGYDKAANSLLEKIEVFYELQCGAISPEEMSVCLFELFAEWLDGTETDDVLYHQEDDLFPMIEM
MQQESVDSKKVLRPDGSFMEWFLTPNVQFGLLAILTVLGLFLPFTMLLSILILPPLMVAFTDRKFRPPLR
MPKDCEMFDDTLTTETQAEYRLGPIRIPHKVRERKKAKGILYVGYERGRLFGRELWLNMTDLLRHMVFFG
TTGSGKTETFYGFIVNFLLWCRGYCLSDGKADNKLAFATWSLARRFGREDDYYVLNLLTGSIDRFVNLVK
QESIPAQSNSVNLFSVAPPTFIIQLMESMLPQVGGDSAQWQDTAKAMMSALINALCYKRARGELLLSQRT
IQKNMSLPAMAALYVEAKKNGWHSEGYAALESYLENTPGFLLANAEYPETWEARAFEQHNYMSRQFLKTL
SLFNETYGHVFPEDSGDIRMDDIFHNDRILIVMIPSLELSRGEAATLGRLYVTLQRMTISKDLGYQLEGK
KEEVLLTHALNNQAPYGLIYDELGQYFTSGMDTLSAQMRSLEKMGVFSSQDHPSLARGANGEVDSLIANT
RVKYFESIEDRKTFEILRETVGQDYYSELSGQEAEHGTFSKTVREGDLYQIREKDRVNIRELRKQTEGQG
VISFQDALVRSAAFYIPDNEKFSSELPMRINRFIEVLPPSPATLYSIYPERKDDELAETNPDAMRFPPIK
LFGYDKAANSLLEKIEVFYELQCGAISPEEMSVCLFELFAEWLDGTETDDVLYHQEDDLFPMIEM
Protein domains
No domain identified.
Protein structure
No available structure.
T4SS
T4SS were predicted by using oriTfinder2.
Region 1: 47444..66595
| Locus tag | Coordinates | Strand | Size (bp) | Protein ID | Product | Description |
|---|---|---|---|---|---|---|
| B8P98_RS30220 (B8P98_30195) | 42528..42839 | + | 312 | WP_004187333 | hypothetical protein | - |
| B8P98_RS30225 (B8P98_30200) | 42972..43508 | + | 537 | WP_004206920 | hypothetical protein | - |
| B8P98_RS30230 (B8P98_30205) | 44009..44374 | - | 366 | WP_004206922 | hypothetical protein | - |
| B8P98_RS30235 (B8P98_30210) | 44650..44967 | + | 318 | WP_004206923 | plasmid mobilization protein MobA | - |
| B8P98_RS30240 (B8P98_30215) | 44954..46933 | + | 1980 | WP_004206924 | TraI/MobA(P) family conjugative relaxase | - |
| B8P98_RS30245 (B8P98_30220) | 46947..47447 | + | 501 | WP_004206925 | DotD/TraH family lipoprotein | - |
| B8P98_RS30250 (B8P98_30225) | 47444..48223 | + | 780 | WP_004187315 | type IV secretory system conjugative DNA transfer family protein | traI |
| B8P98_RS30255 (B8P98_30230) | 48234..49397 | + | 1164 | WP_004206926 | plasmid transfer ATPase TraJ | virB11 |
| B8P98_RS30260 (B8P98_30235) | 49387..49647 | + | 261 | WP_004187310 | IcmT/TraK family protein | traK |
| B8P98_RS31795 | 49672..52932 | + | 3261 | WP_219725162 | LPD7 domain-containing protein | - |
| B8P98_RS30270 (B8P98_30245) | 52898..53410 | + | 513 | WP_011091071 | hypothetical protein | traL |
| B8P98_RS30275 (B8P98_30250) | 53411..53623 | - | 213 | WP_305953721 | Hha/YmoA family nucleoid-associated regulatory protein | - |
| B8P98_RS30280 (B8P98_30255) | 53595..54005 | - | 411 | WP_004187465 | H-NS family nucleoid-associated regulatory protein | - |
| B8P98_RS30285 (B8P98_30260) | 54073..54786 | + | 714 | WP_223294958 | DotI/IcmL family type IV secretion protein | traM |
| B8P98_RS30290 (B8P98_30265) | 54795..55946 | + | 1152 | WP_015062843 | DotH/IcmK family type IV secretion protein | traN |
| B8P98_RS30295 (B8P98_30270) | 55958..57307 | + | 1350 | WP_004206932 | conjugal transfer protein TraO | traO |
| B8P98_RS30300 (B8P98_30275) | 57319..58023 | + | 705 | WP_004206933 | conjugal transfer protein TraP | traP |
| B8P98_RS30305 (B8P98_30280) | 58047..58577 | + | 531 | WP_004187478 | conjugal transfer protein TraQ | traQ |
| B8P98_RS30310 (B8P98_30285) | 58594..58983 | + | 390 | WP_011154472 | DUF6750 family protein | traR |
| B8P98_RS30315 (B8P98_30290) | 59029..59523 | + | 495 | WP_004187480 | hypothetical protein | - |
| B8P98_RS30320 (B8P98_30295) | 59520..62570 | + | 3051 | WP_011154474 | conjugative transfer protein | traU |
| B8P98_RS30325 (B8P98_30300) | 62567..63775 | + | 1209 | WP_004187486 | conjugal transfer protein TraW | traW |
| B8P98_RS30330 (B8P98_30305) | 63772..64422 | + | 651 | WP_004187488 | hypothetical protein | - |
| B8P98_RS30335 (B8P98_30310) | 64415..66595 | + | 2181 | WP_095033693 | DotA/TraY family protein | traY |
| B8P98_RS30340 (B8P98_30315) | 66598..67251 | + | 654 | WP_004206935 | hypothetical protein | - |
| B8P98_RS30345 (B8P98_30320) | 67325..67555 | + | 231 | WP_004187496 | IncL/M type plasmid replication protein RepC | - |
Host bacterium
| ID | 19593 | GenBank | NZ_CP022826 |
| Plasmid name | pKPN1705-3 | Incompatibility group | IncL/M |
| Plasmid size | 67851 bp | Coordinate of oriT [Strand] | 44542..44646 [+] |
| Host baterium | Klebsiella quasivariicola strain KPN1705 |
Cargo genes
| Drug resistance gene | ant(2'')-Ia, blaSHV-30, qnrS1 |
| Virulence gene | - |
| Metal resistance gene | - |
| Degradation gene | - |
| Symbiosis gene | - |
| Anti-CRISPR | - |