Detailed information of oriT

oriT

The information of the oriT region


oriTDB ID   119162
Name   oriT_pKPN1705-2 in_silico
Organism   Klebsiella quasivariicola strain KPN1705
Sequence Completeness      -
NCBI accession of oriT (coordinates [strand])   NZ_CP022825 (58513..58561 [-], 49 nt)
oriT length   49 nt
IRs (inverted repeats)      6..13, 16..23  (GCAAAATT..AATTTTGC)
Location of nic site      32..33
Conserved sequence flanking the
  nic site    		  TGTGTGGTGA
Note   Predicted by oriTfinder 2.0

  oriT sequence  


Download         Length: 49 nt

>oriT_pKPN1705-2
AATCTGCAAAATTTTAATTTTGCGTAGTGTGTGGTGATTTTTTGGTGAG

Visualization of oriT structure

  oriT secondary structure

Predicted by RNAfold.

Download structure file


Relaxase

ID   12119 GenBank   WP_095033690
Name   traI_B8P98_RS29865_pKPN1705-2 insolico UniProt ID   _
Length   1752 a.a. PDB ID   
Note   Predicted by oriTfinder 2.0

  Relaxase protein sequence


Download         Length: 1752 a.a.        Molecular weight: 190110.97 Da        Isoelectric Point: 6.3071

>WP_095033690.1 conjugative transfer relaxase/helicase TraI [Klebsiella quasivariicola]
MLSFSQVKSAGSAGNYYTDKDNYYVIGSMDERWQGKGAEALGLEGKVDKQVFTELLQGKLPDGSDLTRIQ
DGVNKHRPGYDLTFSAPKSVSMLAMLGGDKRLIDAHNRAVTVALNQVESLASTRVKRDGVSETVLTGNLI
IARFNHDTSRAQDPQIHTHSVVINATQNGDKWQTLASDTVGKTGFSENILANRIALGKIYQNSLRADVES
MGYKTVDAGKNGMWEMEGVPVESFSTRSQELREAAGPDASLKSRDVAALDTRKSKEAIDPAEKMVEWMNT
LKETGFDIRGYREAADARAAELARAPAAPVNTDGPDITDVVTKAIAGLSDRKVQFTYADLLARTVGQLEA
KDGVFELARKGIDAAIEREQLIPLDREKGLFTSNIHVLDELAVKALSQEVQRQNHVSVTPDASVVRQVPF
SDAVSVLAQDRPVMGIVSGQGGAAGQRERVAELTLMAREQGRDVHILAADNRSRDFLAGDVRLAGETVTG
KSALQDGTAFIPGGTLIVDQAEKLSLKETISLLDGAMRHNVQVLLSDSGKRSGTGSALTVLKDSGVNTYR
WQGGHQTTADIISEPDKGARYSRLAQEFAVSVREGLESVAQISGTREQSVLNGLIRDSLRNEGVLGEKDT
TITALTPVWLDSKSRGVRDYYREGMVMESWDPETRTHDRFVIDRVTASSNMLTLKDREGGRLDLKVSAVD
SQWTLFRADTLPVAEGERLAVLGKIPDTRLKGGESITVMKVEDGQLTVQRPGQKTTQTLAVGAGVFDGIK
IGHGWVESPGRSVSETATVFASVTQRELDNATLNQLAQSGSHLRLYSAQDAARTTEKLSRHTAFSVVSEQ
LKTRSGETDLDAAIAQQKAGLRTPAEQAIHLAIPLLESEKLTFSRPQLLATALETGGGKVPMADIDTTIQ
AQIRSGQLLNVPVAHGYGNDLLISRQTWDAEKSILTHVLEGKDAVAPLMDRVPASLMTDLTAGQRAATRM
ILESTDRFTVVQGYAGVGKTTQFRAVMSAISLLPEETRPRVIGLAPTHRAVGEMQSAGVDARTTASFLHD
TQLLQRNGQTPDFSNTLFLLDESSMVGLADMAKAHSLIVAGGGRAVSSGDNDQLQPIAPGQPFRLMQQRS
AADIAIMKEIVRQVPELRPAVYSLIERDVHRALTTIEQVTPEQVPRKEGAWAPGSSVVEFTPAQEKAIQK
ALSEGKTLPAGQPATLYEALVKDYTGRTPEAQSQTLVITHLNKDRRALNSLIHGARRENGETGKEEITLP
VLVTSNIRDGELRKLSTWTAHKEAVALVDNVYHRISKVDKDNQMITLTDSEGKERYISPREASAEGVTLY
RQEKITVSQGDRMRFSKSDLERGYVANSIWEVQSVAGDSVTLSDGKTTRTLTPKADQAQQHIDLAYAITA
HGAQGASEPYAIALEGVAGGREQMASFESAYVALSRMKQHVQVYTDSREGWIKAIKHSPEKATAHDILEP
RNDRAVKSADLLFGRARLLDETAAGRAALQQSGLAQGNSPGKFISPGKKYPQPHVALPAFDKNGKAAGIW
LSPLTDRDGRLEAIGGEGRIMGNDAARFVALQNSRNGESLLAGNMGEGVRMARDNPDTGVVVRLAGDDRP
WNPGAMTGGRVWAEPAPVAPVPQAGADIILPPEVLAQRAAEEQQRREMEKQAEQTAREVAGEARKAGEPV
DRVKEVIGDVIRGLERDRPGPEKTTLPDDPQFRRQEAAIQQVASESLQRERLQAVERDMVRDLNREKTLG
GD

  Protein domains


Predicted by InterproScan.

single-stranded DNA binding TraI N-terminal subdomain (ssDNA_TraI_N)

(576-628)

TrwC relaxase (TrwC)

(10-283)

DNA relaxase TraI 2B/2B-like domain (TraI_2B)

(634-713)

AAA domain (AAA_30)

(970-1156)

DNA helicase TraI (TraI_C)

(1432-1554)


  Protein structure



No available structure.




Auxiliary protein

ID   6935 GenBank   WP_087829209
Name   WP_087829209_pKPN1705-2 insolico UniProt ID   _
Length   129 a.a. PDB ID   _
Note   Predicted by oriTfinder 2.0

  Auxiliary protein sequence


Download         Length: 129 a.a.        Molecular weight: 14907.97 Da        Isoelectric Point: 4.5248

>WP_087829209.1 MULTISPECIES: conjugal transfer relaxosome DNA-binding protein TraM [Enterobacteriaceae]
MGKVQAYPSDEVCEKINAIVEKRRMEGAKDKDVSFSSISTMLLELGLRVYEAQMERKESGFNQMAFNRAL
LESVVKTQFSVNKILGIECLSPHVKDDPRWQWKSMVLNIQEDVQEAMRNFFPDEDAEEE

  Protein domains


Predicted by InterproScan.

TraM protein, DNA-binding (Tra_M)

(1-124)


  Protein structure



No available structure.




T4CP

ID   14196 GenBank   WP_095033689
Name   traD_B8P98_RS29860_pKPN1705-2 insolico UniProt ID   _
Length   761 a.a. PDB ID   _
Note   Predicted by oriTfinder 2.0

  T4CP protein sequence


Download         Length: 761 a.a.        Molecular weight: 85308.33 Da        Isoelectric Point: 4.9677

>WP_095033689.1 MULTISPECIES: type IV conjugative transfer system coupling protein TraD [Klebsiella/Raoultella group]
MSFNAKDMTQGGQIANMRFRMFGQIANIIFYVLFILFWVLCGLMLMYRLSWQTFVNGCVYWWCTTLGPMR
DIIRSQPVYTIQYYGQSLEYTSEQILADKYTIWCGEQLWTSFIFAAVVSLVICIVTFFVASWVLGRQGKQ
QSEDENTGGRQLSDKPKEVARQMKRDGMASDIKIGDLPILLNSEIQNFCLHGTVGSGKSEVIRRLLNYVR
ARGDMAIIYDRSCEFVKSYYDPSLDKILNPLDSRCAAWDLWKECLTLPDFDNISNTLIPMGTKEDPFWQG
SGRTIFAEGAYLMREDKDRSYEKLVDTMLSIKIDKLRAYLQNTPAANLVEEKIEKTAISIRAVLTNYVKA
IRYLQGIEKNGEPFTIRDWMRGVREDQPNGWLFISSNADTHASLKPVISMWLSIAIRGLLAMGENRNRRV
WIFADELPTLHKLPDLVEILPEARKFGGCYVFGIQSYAQLEDIYGVKPAATLFDVMNTRAFFRSPSKEIA
EFAAGEIGEKEILKASEQYSYGADPVRDGVSTGKEKERETLVSYSDIQTLPDLSCYVTLPGPYPAVKLAL
KYKPRPKIAEGFIPRSLDARVDARLSALLEAREAEGSLARALFTPDAPEPGPADTDSHAGEQPEPAEVTV
SPAPVKATQTTKMPAEEPSVRATEPPVLRVTTVPLIKPKAAGTAATASSAGTPVAPAGGTEQELVQHSTE
QGRDMLPAGMNEDGVIEDMQAYDAWLADEQTLRDMQRREEVNINHSHRHDEQDDVEIGGNF

  Protein domains


Predicted by InterproScan.

F sex factor protein N terminal (TraD_N)

(32-128)

Type IV secretion-system coupling protein DNA-binding domain (TrwB_AAD_bind)

(172-560)

  Protein structure



No available structure.




T4SS

T4SS were predicted by using oriTfinder2.

Region 1: 60975..86731

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
B8P98_RS29675 (B8P98_29650) 57393..57923 + 531 WP_015632491 antirestriction protein -
B8P98_RS29680 (B8P98_29655) 57961..58368 - 408 WP_020805750 transglycosylase SLT domain-containing protein -
B8P98_RS29685 (B8P98_29660) 58873..59262 + 390 WP_087829209 conjugal transfer relaxosome DNA-binding protein TraM -
B8P98_RS29690 (B8P98_29665) 59501..60175 + 675 WP_095033684 hypothetical protein -
B8P98_RS29695 (B8P98_29670) 60367..60531 + 165 WP_032444506 TraY domain-containing protein -
B8P98_RS29700 (B8P98_29675) 60593..60961 + 369 WP_087829211 type IV conjugative transfer system pilin TraA -
B8P98_RS29705 (B8P98_29680) 60975..61280 + 306 WP_087829212 type IV conjugative transfer system protein TraL traL
B8P98_RS29710 (B8P98_29685) 61300..61866 + 567 WP_064373744 type IV conjugative transfer system protein TraE traE
B8P98_RS29715 (B8P98_29690) 61853..62593 + 741 WP_020804840 type-F conjugative transfer system secretin TraK traK
B8P98_RS29720 (B8P98_29695) 62593..64017 + 1425 WP_095033685 F-type conjugal transfer pilus assembly protein TraB traB
B8P98_RS29725 (B8P98_29700) 64010..64606 + 597 WP_023316398 conjugal transfer pilus-stabilizing protein TraP -
B8P98_RS29730 (B8P98_29705) 64629..65198 + 570 WP_023316399 type IV conjugative transfer system lipoprotein TraV traV
B8P98_RS29735 (B8P98_29710) 65330..65740 + 411 WP_095033686 hypothetical protein -
B8P98_RS29740 (B8P98_29715) 65745..66029 + 285 WP_095033692 hypothetical protein -
B8P98_RS29745 (B8P98_29720) 66053..66271 + 219 WP_004171484 hypothetical protein -
B8P98_RS29750 (B8P98_29725) 66272..66589 + 318 WP_071041197 hypothetical protein -
B8P98_RS29755 (B8P98_29730) 66656..67060 + 405 WP_071041196 hypothetical protein -
B8P98_RS29760 (B8P98_29735) 67057..67347 + 291 WP_071041194 hypothetical protein -
B8P98_RS29765 (B8P98_29740) 67355..67753 + 399 WP_167382710 hypothetical protein -
B8P98_RS29770 (B8P98_29745) 67825..70464 + 2640 WP_060446930 type IV secretion system protein TraC virb4
B8P98_RS29775 (B8P98_29750) 70464..70853 + 390 WP_020314627 type-F conjugative transfer system protein TrbI -
B8P98_RS29780 (B8P98_29755) 70853..71479 + 627 WP_020314628 type-F conjugative transfer system protein TraW traW
B8P98_RS29785 (B8P98_29760) 71523..72482 + 960 WP_029497356 conjugal transfer pilus assembly protein TraU traU
B8P98_RS29790 (B8P98_29765) 72495..73133 + 639 WP_095033687 type-F conjugative transfer system pilin assembly protein TrbC trbC
B8P98_RS29795 (B8P98_29770) 73192..75147 + 1956 WP_020803160 type-F conjugative transfer system mating-pair stabilization protein TraN traN
B8P98_RS29800 (B8P98_29775) 75180..75461 + 282 WP_022644736 hypothetical protein -
B8P98_RS29805 (B8P98_29780) 75451..75678 + 228 WP_012540032 conjugal transfer protein TrbE -
B8P98_RS29810 (B8P98_29785) 75689..76015 + 327 WP_020323521 hypothetical protein -
B8P98_RS29815 (B8P98_29790) 76036..76788 + 753 WP_023316406 type-F conjugative transfer system pilin assembly protein TraF traF
B8P98_RS29825 (B8P98_29800) 77243..77482 + 240 WP_023316407 type-F conjugative transfer system pilin chaperone TraQ -
B8P98_RS29830 (B8P98_29805) 77454..78011 + 558 WP_020804677 type-F conjugative transfer system pilin assembly thiol-disulfide isomerase TrbB traF
B8P98_RS29835 (B8P98_29810) 78057..78500 + 444 WP_020804675 F-type conjugal transfer protein TrbF -
B8P98_RS29840 (B8P98_29815) 78478..79857 + 1380 WP_162495503 conjugal transfer pilus assembly protein TraH traH
B8P98_RS29845 (B8P98_29820) 79857..82706 + 2850 WP_095033688 conjugal transfer mating-pair stabilization protein TraG traG
B8P98_RS29850 (B8P98_29825) 82712..83239 + 528 WP_020803849 hypothetical protein -
B8P98_RS31790 83422..84090 + 669 WP_020803846 hypothetical protein -
B8P98_RS30585 84114..84374 + 261 WP_020803850 hypothetical protein -
B8P98_RS29860 (B8P98_29835) 84446..86731 + 2286 WP_095033689 type IV conjugative transfer system coupling protein TraD virb4

Host bacterium

ID   19592 GenBank   NZ_CP022825
Plasmid name   pKPN1705-2 Incompatibility group   IncFIA
Plasmid size   97896 bp Coordinate of oriT [Strand]   58513..58561 [-]
Host baterium   Klebsiella quasivariicola strain KPN1705

Cargo genes

Drug resistance gene   -
Virulence gene   -
Metal resistance gene   -
Degradation gene   -
Symbiosis gene   -
Anti-CRISPR   AcrIE9