Detailed information of oriT
oriT
The information of the oriT region
| oriTDB ID | 119159 |
| Name | oriT_FDAARGOS_93|unnamed1  |
| Organism | Klebsiella quasipneumoniae strain FDAARGOS_93 |
| Sequence Completeness | - |
| NCBI accession of oriT (coordinates [strand]) | NZ_CP014072 (82..186 [+], 105 nt) |
| oriT length | 105 nt |
| IRs (inverted repeats) | 32..37, 40..45 (GCCGGC..GCCGGC) |
| Location of nic site | _ |
| Conserved sequence flanking the nic site |
_ |
| Note | Predicted by oriTfinder 2.0 |
oriT sequence
Download Length: 105 nt
>oriT_FDAARGOS_93|unnamed1
AGTACGGGACAAGATGTGTTTTTGGTACACCGCCGGCACGCCGGCAGTGACGCAAAAACGTCTTGGTCAGGGCAAGCCCCGACACCCCCTAACGAGGTTAGCTAT
AGTACGGGACAAGATGTGTTTTTGGTACACCGCCGGCACGCCGGCAGTGACGCAAAAACGTCTTGGTCAGGGCAAGCCCCGACACCCCCTAACGAGGTTAGCTAT
Visualization of oriT structure
oriT secondary structure
Predicted by RNAfold.
Download structure file
Relaxase
| ID | 12116 | GenBank | WP_004206924 |
| Name | traI_AL473_RS26425_FDAARGOS_93|unnamed1 |
UniProt ID | A0A2V4FJ40 |
| Length | 659 a.a. | PDB ID | |
| Note | Predicted by oriTfinder 2.0 | ||
Relaxase protein sequence
Download Length: 659 a.a. Molecular weight: 75087.84 Da Isoelectric Point: 10.0285
>WP_004206924.1 MULTISPECIES: TraI/MobA(P) family conjugative relaxase [Enterobacterales]
MVIPKIIEGRRDKKSSFGQLIKYMADKPSQELTDTVQPTPETALAVKSDMFEGLNNYLTRKQKVISTPVD
VEPGVQRVVVGDVTCQYNTFSLDGAAQEMNSVSQQSTRCKDPVMHYVLSWPDYEKPNDDQVFDSVKFTLA
SMGMSDHQYVAAIHRDTDNLHVHVAVNRINPQTYKAASSSFTKDTLHQACRLLELKNGWSHSNGAYVVND
RQQIVRNPHSKKERGNWRSLDRINKMENKEGVETLYRYIVGDEQVGGSRQNLIHVSAGLREAKSWDDVHK
TFAGIGLRVEKAQGKKGYVITHEHQNQKTAVKASLVFNKAQYTLKSMEERFGEYQPSHIEPAKVSVFKTA
YTPGAYRRDANKRLQRKIERAEERMLLKGRYRAYRNNLPIYSPDKDRIADEYRKIAQHTRLVKNNVRHSV
SDPHTRKLMYNLAEFKRLQAVANLRLSLREERNGFRAANPRLSYREWVEQEALKGDKAALSQMRGFAYSS
RKKEKYKQQLVEQIGFNRTFNAITSHDRDDVAVMASARHGVKPRLLKDGTVIFERDGKPVAADRGHIVLT
ESNGIDKEKTADLAIALTIAGKAKSVRVDGDGEFKELCCNRIVDAAVNHNHPVAQGITFTDAAQQAYAQN
EKHRLIREQNNSKNEMQLRSESDDKFNPK
MVIPKIIEGRRDKKSSFGQLIKYMADKPSQELTDTVQPTPETALAVKSDMFEGLNNYLTRKQKVISTPVD
VEPGVQRVVVGDVTCQYNTFSLDGAAQEMNSVSQQSTRCKDPVMHYVLSWPDYEKPNDDQVFDSVKFTLA
SMGMSDHQYVAAIHRDTDNLHVHVAVNRINPQTYKAASSSFTKDTLHQACRLLELKNGWSHSNGAYVVND
RQQIVRNPHSKKERGNWRSLDRINKMENKEGVETLYRYIVGDEQVGGSRQNLIHVSAGLREAKSWDDVHK
TFAGIGLRVEKAQGKKGYVITHEHQNQKTAVKASLVFNKAQYTLKSMEERFGEYQPSHIEPAKVSVFKTA
YTPGAYRRDANKRLQRKIERAEERMLLKGRYRAYRNNLPIYSPDKDRIADEYRKIAQHTRLVKNNVRHSV
SDPHTRKLMYNLAEFKRLQAVANLRLSLREERNGFRAANPRLSYREWVEQEALKGDKAALSQMRGFAYSS
RKKEKYKQQLVEQIGFNRTFNAITSHDRDDVAVMASARHGVKPRLLKDGTVIFERDGKPVAADRGHIVLT
ESNGIDKEKTADLAIALTIAGKAKSVRVDGDGEFKELCCNRIVDAAVNHNHPVAQGITFTDAAQQAYAQN
EKHRLIREQNNSKNEMQLRSESDDKFNPK
Protein domains
Predicted by InterproScan.
Protein structure
| Source | ID | Structure |
|---|---|---|
| AlphaFold DB | A0A2V4FJ40 |
T4CP
| ID | 14193 | GenBank | WP_004206885 |
| Name | t4cp2_AL473_RS26690_FDAARGOS_93|unnamed1 |
UniProt ID | _ |
| Length | 695 a.a. | PDB ID | _ |
| Note | Predicted by oriTfinder 2.0 | ||
T4CP protein sequence
Download Length: 695 a.a. Molecular weight: 79538.91 Da Isoelectric Point: 4.8317
>WP_004206885.1 MULTISPECIES: conjugal transfer protein TrbC [Enterobacterales]
MQQESVDSKKVLRPDGSFMEWFLTPNVQFGLLAILTVLGLFLPFTMLLSILILPPLMVAFTDRKFRPPLR
MPKDCEMFDDTLTTETQAEYRLGPIRIPHKVRERKKAKGILYVGYERGRLFGRELWLNMTDLLRHMVFFG
TTGSGKTETFYGFIVNFLLWCRGYCLSDGKADNKLAFATWSLARRFGREDDYYVLNLLTGSIDRFVNLVK
QESIPAQSNSVNLFSVAPPTFIIQLMESMLPQVGGDSAQWQDTAKAMMSALINALCYKRARGELLLSQRT
IQKNMSLPAMAALYVEAKKNGWHSEGYAALESYLENTPGFLLANAEYPETWEARAFEQHNYMSRQFLKTL
SLFNETYGHVFPEDSGDIRMDDIFHNDRILIVMIPSLELSRGEAATLGRLYVTLQRMTISKDLGYQLEGK
KEEVLLTHALNNQAPYGLIYDELGQYFTSGMDTLSAQMRSLEKMGVFSSQDHPSLARGANGEVDSLIANT
RVKYFESIEDRKTFEILRETVGQDYYSELSGQEAEHGTFSKTVREGDLYQIREKDRVNIRELRKQTEGQG
VISFQDALVRSAAFYIPDNEKFSSELPMRINRFIEVLPPSPATLYSIYPERKDDELAETNPDAMRFPPIK
LFGYDKAANSLLEKIEVFYELQCGAISPEEMSVCLFELFAEWLDGTETDDVLYHQEDDLFPMIEM
MQQESVDSKKVLRPDGSFMEWFLTPNVQFGLLAILTVLGLFLPFTMLLSILILPPLMVAFTDRKFRPPLR
MPKDCEMFDDTLTTETQAEYRLGPIRIPHKVRERKKAKGILYVGYERGRLFGRELWLNMTDLLRHMVFFG
TTGSGKTETFYGFIVNFLLWCRGYCLSDGKADNKLAFATWSLARRFGREDDYYVLNLLTGSIDRFVNLVK
QESIPAQSNSVNLFSVAPPTFIIQLMESMLPQVGGDSAQWQDTAKAMMSALINALCYKRARGELLLSQRT
IQKNMSLPAMAALYVEAKKNGWHSEGYAALESYLENTPGFLLANAEYPETWEARAFEQHNYMSRQFLKTL
SLFNETYGHVFPEDSGDIRMDDIFHNDRILIVMIPSLELSRGEAATLGRLYVTLQRMTISKDLGYQLEGK
KEEVLLTHALNNQAPYGLIYDELGQYFTSGMDTLSAQMRSLEKMGVFSSQDHPSLARGANGEVDSLIANT
RVKYFESIEDRKTFEILRETVGQDYYSELSGQEAEHGTFSKTVREGDLYQIREKDRVNIRELRKQTEGQG
VISFQDALVRSAAFYIPDNEKFSSELPMRINRFIEVLPPSPATLYSIYPERKDDELAETNPDAMRFPPIK
LFGYDKAANSLLEKIEVFYELQCGAISPEEMSVCLFELFAEWLDGTETDDVLYHQEDDLFPMIEM
Protein domains
No domain identified.
Protein structure
No available structure.
T4SS
T4SS were predicted by using oriTfinder2.
Region 1: 2984..22180
| Locus tag | Coordinates | Strand | Size (bp) | Protein ID | Product | Description |
|---|---|---|---|---|---|---|
| AL473_RS26420 (AL473_26420) | 190..507 | + | 318 | WP_004206923 | plasmid mobilization protein MobA | - |
| AL473_RS26425 (AL473_26425) | 494..2473 | + | 1980 | WP_004206924 | TraI/MobA(P) family conjugative relaxase | - |
| AL473_RS26430 (AL473_26430) | 2487..2987 | + | 501 | WP_004206925 | DotD/TraH family lipoprotein | - |
| AL473_RS26435 (AL473_26435) | 2984..3763 | + | 780 | WP_004187315 | type IV secretory system conjugative DNA transfer family protein | traI |
| AL473_RS26440 (AL473_26440) | 3774..4937 | + | 1164 | WP_004206926 | plasmid transfer ATPase TraJ | virB11 |
| AL473_RS26445 (AL473_26445) | 4927..5187 | + | 261 | WP_004187310 | IcmT/TraK family protein | traK |
| AL473_RS28610 | 5212..8517 | + | 3306 | WP_219818010 | LPD7 domain-containing protein | - |
| AL473_RS26455 (AL473_26455) | 8483..8995 | + | 513 | WP_011091071 | hypothetical protein | traL |
| AL473_RS26460 (AL473_26460) | 8996..9208 | - | 213 | WP_305953721 | Hha/YmoA family nucleoid-associated regulatory protein | - |
| AL473_RS26465 (AL473_26465) | 9180..9590 | - | 411 | WP_004187465 | H-NS family nucleoid-associated regulatory protein | - |
| AL473_RS26470 (AL473_26470) | 9658..10371 | + | 714 | WP_223294958 | DotI/IcmL family type IV secretion protein | traM |
| AL473_RS26475 (AL473_26475) | 10380..11531 | + | 1152 | WP_015062843 | DotH/IcmK family type IV secretion protein | traN |
| AL473_RS26480 (AL473_26480) | 11543..12892 | + | 1350 | WP_004206932 | conjugal transfer protein TraO | traO |
| AL473_RS26485 (AL473_26485) | 12904..13608 | + | 705 | WP_004206933 | conjugal transfer protein TraP | traP |
| AL473_RS26490 (AL473_26490) | 13632..14162 | + | 531 | WP_004187478 | conjugal transfer protein TraQ | traQ |
| AL473_RS26495 (AL473_26495) | 14179..14568 | + | 390 | WP_011154472 | DUF6750 family protein | traR |
| AL473_RS26500 (AL473_26500) | 14614..15108 | + | 495 | WP_004187480 | hypothetical protein | - |
| AL473_RS26505 (AL473_26505) | 15105..18155 | + | 3051 | WP_004206934 | hypothetical protein | traU |
| AL473_RS26510 (AL473_26510) | 18152..19360 | + | 1209 | WP_004187486 | conjugal transfer protein TraW | traW |
| AL473_RS26515 (AL473_26515) | 19357..20007 | + | 651 | WP_004187488 | hypothetical protein | - |
| AL473_RS26520 (AL473_26520) | 20000..22180 | + | 2181 | WP_004187492 | DotA/TraY family protein | traY |
| AL473_RS26525 (AL473_26525) | 22183..22836 | + | 654 | WP_004206935 | hypothetical protein | - |
| AL473_RS26530 (AL473_26530) | 22910..23140 | + | 231 | WP_004187496 | IncL/M type plasmid replication protein RepC | - |
| AL473_RS26535 (AL473_26535) | 23437..24492 | + | 1056 | WP_015062846 | plasmid replication initiator RepA | - |
| AL473_RS26540 (AL473_26540) | 25664..26197 | - | 534 | Protein_24 | IS110-like element IS4321 family transposase | - |
Host bacterium
| ID | 19589 | GenBank | NZ_CP014072 |
| Plasmid name | FDAARGOS_93|unnamed1 | Incompatibility group | IncL/M |
| Plasmid size | 76312 bp | Coordinate of oriT [Strand] | 82..186 [+] |
| Host baterium | Klebsiella quasipneumoniae strain FDAARGOS_93 |
Cargo genes
| Drug resistance gene | ant(2'')-Ia, aadA6, blaOXA-2, qacE, sul1, blaSHV-18 |
| Virulence gene | - |
| Metal resistance gene | merE, merD, merA, merC, merP, merT, merR |
| Degradation gene | - |
| Symbiosis gene | - |
| Anti-CRISPR | - |