Detailed information of oriT

oriT

The information of the oriT region

• oriTDB ID: 119149
• Name: oriT_On28M|unnamed
• Organism: Citrobacter sp. On28M
• Sequence Completeness: -
• NCBI accession of oriT (coordinates [strand]): NZ_JAABKH010000009 (6781..6862 [-], 82 nt)
• oriT length: 82 nt
• IRs (inverted repeats): _
• Location of nic site: _
• Conserved sequence flanking the nic site: _
• Note: Predicted by oriTfinder 2.0

oriT sequence

• Length: 82 nt

>oriT_On28M|unnamed
GGGACAGGATATGTTTTGTTGAACCGCCTGCACGCAGTCGGCTTCAAAAAACATCCTGGTCAGGGCGAAGCCCCGACACCCC

oriT secondary structure

Predicted by RNAfold.

Relaxase

traI_CTBON28M_RS24120_On28M|unnamed

• ID: 12109
• GenBank: WP_219608514
• Name: traI_CTBON28M_RS24120_On28M|unnamed
• UniProt ID: _
• Length: 663 a.a.
• Note: Predicted by oriTfinder 2.0

Relaxase protein sequence

• Length: 663 a.a.
• Molecular weight: 76052.27 Da
• Isoelectric Point: 5.9734

>WP_219608514.1 TraI/MobA(P) family conjugative relaxase [Citrobacter sp. On28M]
MIVIVPEKRRDGKSSFLTLVSYLTLREDVRLAEPVSPDAPVMRMSRSKEAIFDRLVDYIDRSENAVTQTP
VKEFADGRSQVLSGSVACETNCFSLDTASAEMNIVAAQNTRCIDPVYHFILSWPEQDNPVDADIFDCARS
ALHALGMKEHQFVTAIHRDTDHVHCHVAVNRINPVSYRAANLYNDIDTLHKVCRFLEIKYDYTPENGAWV
NENGAVVRNTNDVKSIPRKARQLEYHSDKESLYSYAVNECRERIGEILGSGEASWETLHAELIRAGLDMR
EKGEGLAIYSRHDENVTPIKASTLHPDLTRQCLEKWLGDFEPSPEVVNNFDDDGVYLGSNYPQEFVYDAR
AHVRDQGARSERRLARAVAREDLDARYKAYKNAWVRPKMPDTDSGVQFRALSNRYAWKKEQVRYVFDDPL
IRKLIYRTLELERQKEFEMLRDKVKAEKTQFYSRPENRRLSYWQWVERQAAAQDQAAISRLRGRAYKLKQ
KQRTPGLSENAIVCAVADDIATVNIDGFESRVNRDGTIQYLQDGHVVIQDSGERIEIADPYREEGIHISD
AMLIAEEKSGERLLFSGEGRFVNSACDVVQWFNDGGEKPLPLTDPQQRVMAGYDKPETHSDRPVMTHRIV
DEETYLASRQGARAVDDRETEELKPEKKTHRLR

Protein domains

Predicted by InterproScan.

• Large polyvalent protein-associated domain 7 (LPD7) (517-589)

• Relaxase/Mobilisation nuclease domain (Relaxase) (52-317)

Protein structure

No available structure.

T4CP

trbC_CTBON28M_RS24675_On28M|unnamed

• ID: 14184
• GenBank: WP_219608589
• Name: trbC_CTBON28M_RS24675_On28M|unnamed
• UniProt ID: _
• Length: 731 a.a.
• PDB ID: _
• Note: Predicted by oriTfinder 2.0

T4CP protein sequence

• Length: 731 a.a.
• Molecular weight: 83078.44 Da
• Isoelectric Point: 8.0317

>WP_219608589.1 F-type conjugative transfer protein TrbC [Citrobacter sp. On28M]
MTTGNTQPVDKQRVRQITQSTWIDDYLLAPATVQAGLVVILVLTFLRTWILLPGVFATSIWLLTFFGQLF
RMPLRMPKDIGGFDMTTERENALEFKGPMGLFRFTRRRLTWEKSAGIMCLGHARRRFLGRELWLTRDDCL
RHMQLLATTGSGKTEALLSLQLNSLCMGRGMMFSDGKAETKLAYSIWSLMRRYGQEDNYYVLNFLNGGRD
RFDELLGNDRTRPQSNSINFFGDATATFIIQLMESLLPQVGSNEAGWQDKAKPMLYGLVYALYYKCRKEN
IRLSQSMIQKHLPLKEMADLYIEGKQNGWHDEAVSALEAYLSNLAGFRMELVSKSSEWDQGVYDQHGFLT
QQFSRMLAMFNDVYGHIFSSDAGDIDISDVLHNDRTLVVLIPALELSKSESANLGKLYISAKRMVYARDL
GYQLEGKSRDVLTSAKFYNPFPFTDAHDELASYFAPGMDDLAAQMRSLGVMLVISAQDIQRFVAQFKGEY
QTVNANTLVKWFMAMQDEKDTFELAKATAGKDYYAELGEMKRTPGTVSSSYEEANTTYIREKNRITLDEL
KDLNPGEGFISFKSALVPSSAIYIPDSEKISSKLSLRINRFIDTGKPTEADLVAQNPHLRRLLPPSELEV
SMLLQRTDEIIAGNETHTLPPLMDPILARLMKVALDLDNRCDISYTPTQRGILLFEAARDVLKKRGKKWF
TVKTQPNQLRVSDAMAHRLARKSTAFMPQEQ

Protein domains

Predicted by InterproScan.

• TraM recognition site of TraD and TraG (TraG-D_C) (448-563)

Protein structure

No available structure.

Host bacterium

• ID: 19579
• GenBank: NZ_JAABKH010000009
• Plasmid name: On28M|unnamed
• Incompatibility group: IncFIB
• Plasmid size: 107461 bp
• Coordinate of oriT [Strand]: 6781..6862 [-]
• Host baterium: Citrobacter sp. On28M

Cargo genes

• Drug resistance gene: -
• Virulence gene: paa
• Metal resistance gene: -
• Degradation gene: -
• Symbiosis gene: -
• Anti-CRISPR: AcrIB