Detailed information of oriT
oriT
The information of the oriT region
| oriTDB ID | 119138 |
| Name | oriT_P_PS9952019_2  |
| Organism | Providencia stuartii strain PS9952019BGR isolate PS9952019 |
| Sequence Completeness | - |
| NCBI accession of oriT (coordinates [strand]) | NZ_OY979225 (71386..71491 [+], 106 nt) |
| oriT length | 106 nt |
| IRs (inverted repeats) | 30..35, 41..46 (CCGCCG..CGGCGG) |
| Location of nic site | _ |
| Conserved sequence flanking the nic site |
_ |
| Note | Predicted by oriTfinder 2.0 |
oriT sequence
Download Length: 106 nt
>oriT_P_PS9952019_2
AGTACGGGACAAGATGTGTTTTTGGAGTACCGCCGACACGCGGCGGCCGTCCAAAAACGTCTTGGTCAGGGCAAGCCCCGACACCCCCTAACGAGGTTAGCTATCT
AGTACGGGACAAGATGTGTTTTTGGAGTACCGCCGACACGCGGCGGCCGTCCAAAAACGTCTTGGTCAGGGCAAGCCCCGACACCCCCTAACGAGGTTAGCTATCT
Visualization of oriT structure
oriT secondary structure
Predicted by RNAfold.
Download structure file
Relaxase
| ID | 12101 | GenBank | WP_050868819 |
| Name | traI_VOI40_RS23250_P_PS9952019_2 |
UniProt ID | A0A142BMF1 |
| Length | 659 a.a. | PDB ID | |
| Note | Predicted by oriTfinder 2.0 | ||
Relaxase protein sequence
Download Length: 659 a.a. Molecular weight: 75234.98 Da Isoelectric Point: 10.0285
>WP_050868819.1 MULTISPECIES: TraI/MobA(P) family conjugative relaxase [Enterobacterales]
MVIPKIIEGRRDKKSSFGQLIKYMADKPSQELTDTVQPTPETALAVKSDMFEGLNNYLTRKQKVISTPVD
VEPGVQRVVVGDVTCQYNTFSLDGAAQEMNSVSQQSTRCKDPVMHYVLSWPDYEKPNDDQVFDSVKFTLA
SMGMSDHQYVAAIHRDTDNLHVHVAVNRINPQTYKAASSSFTKDTLHQACRLLELKNGWSHSNGAYVVND
RQQIVRNPHSKKERGNWRSLDRINKMENKEGVETLYRYIVGDEQVGGSRQNLIHVSAGLREAKSWDDVHK
TFADIGLRVEKAQGKKGYVITHEHQNQKTAVKASLVFNKAQYTLKSMEERFGEYQPSHIEPAKVSVFKTA
YTPGAYRRDANKRLQRKIERAEERMLLKGRYRAYRNNLPVYSPDKDRIADEYRKIAQHTRLVKNNVRHSV
SDPHTRKLMYNLAEFKRLQAVANLRLSLREERNGFRAANPRLSYREWVEQEALKGDKAALSQMRGFAYSS
RKKEKYKQQLVEQIGFNRTFNAITSHDRDDVAVMASARHGVKPRLLKDGTVIFERDGKPVAADRGHIVLT
ESNGIDKEKTADLAIALTIAGKAKSVRVDGDGEFKELCCNRIVDAAVNHNHPVAQGITFTDAAQQAYAQN
EKHRLIREQNNSKNEMQFRSERDDKFNPK
MVIPKIIEGRRDKKSSFGQLIKYMADKPSQELTDTVQPTPETALAVKSDMFEGLNNYLTRKQKVISTPVD
VEPGVQRVVVGDVTCQYNTFSLDGAAQEMNSVSQQSTRCKDPVMHYVLSWPDYEKPNDDQVFDSVKFTLA
SMGMSDHQYVAAIHRDTDNLHVHVAVNRINPQTYKAASSSFTKDTLHQACRLLELKNGWSHSNGAYVVND
RQQIVRNPHSKKERGNWRSLDRINKMENKEGVETLYRYIVGDEQVGGSRQNLIHVSAGLREAKSWDDVHK
TFADIGLRVEKAQGKKGYVITHEHQNQKTAVKASLVFNKAQYTLKSMEERFGEYQPSHIEPAKVSVFKTA
YTPGAYRRDANKRLQRKIERAEERMLLKGRYRAYRNNLPVYSPDKDRIADEYRKIAQHTRLVKNNVRHSV
SDPHTRKLMYNLAEFKRLQAVANLRLSLREERNGFRAANPRLSYREWVEQEALKGDKAALSQMRGFAYSS
RKKEKYKQQLVEQIGFNRTFNAITSHDRDDVAVMASARHGVKPRLLKDGTVIFERDGKPVAADRGHIVLT
ESNGIDKEKTADLAIALTIAGKAKSVRVDGDGEFKELCCNRIVDAAVNHNHPVAQGITFTDAAQQAYAQN
EKHRLIREQNNSKNEMQFRSERDDKFNPK
Protein domains
Predicted by InterproScan.
Protein structure
| Source | ID | Structure |
|---|---|---|
| AlphaFold DB | A0A142BMF1 |
T4CP
| ID | 14175 | GenBank | WP_275158519 |
| Name | t4cp2_VOI40_RS22980_P_PS9952019_2 |
UniProt ID | _ |
| Length | 619 a.a. | PDB ID | _ |
| Note | Predicted by oriTfinder 2.0 | ||
T4CP protein sequence
Download Length: 619 a.a. Molecular weight: 70770.27 Da Isoelectric Point: 4.7551
>WP_275158519.1 conjugal transfer protein TrbC [Providencia stuartii]
MFDDTLTTETQAEYRLGPIRIPHKVRERKKAKGILYVGYERGRLFGRELWLNMTDLLRHMVFFGTTGSGK
TETFYGFIVNFLLWCRGYCLSDGKADNKLAFATWSLARRFGREDDYYVLNLLTGSIDRFVNLVKQESIPA
QSNSVNLFSVAPPTFIIQLMESMLPQVGGDSAQWQDTAKAMMSALINALCYKRARGELLLSQRTIQKNMS
LPAMAALYVEAKKNGWHSEGYAALESYLENTPGFLLANAEYPETWEARAFEQHNYMSRQFLKTLSLFNET
YGHVFPEDSGDIRMDDIFHNDRILIVMIPSLELSRGEAATLGRLYVTLQRMTISKDLGYQLEGKKEEVLL
THALNNQAPYGLIYDELGQYFTSGMDTLSAQMRSLEKMGVFSSQDHPSLARGANGEVDSLIANTRVKYFE
SIEDRKTFEILRETVGQDYYSELSGQEAEHGTFSKTVREGDLYQIREKDRVNIRELRKQTEGQGVISFQD
ALVRSAAFYIPDNEKFSSELPMRINRFIEVLPPSPATLYSIYPERKDDELAETNPDAMHFPPIKLFGYDK
AANSLLEKIEVFYELQCGAISPEEMSVCLFELFADWLDGTDTDDVLYHQEDDLFPMIEM
MFDDTLTTETQAEYRLGPIRIPHKVRERKKAKGILYVGYERGRLFGRELWLNMTDLLRHMVFFGTTGSGK
TETFYGFIVNFLLWCRGYCLSDGKADNKLAFATWSLARRFGREDDYYVLNLLTGSIDRFVNLVKQESIPA
QSNSVNLFSVAPPTFIIQLMESMLPQVGGDSAQWQDTAKAMMSALINALCYKRARGELLLSQRTIQKNMS
LPAMAALYVEAKKNGWHSEGYAALESYLENTPGFLLANAEYPETWEARAFEQHNYMSRQFLKTLSLFNET
YGHVFPEDSGDIRMDDIFHNDRILIVMIPSLELSRGEAATLGRLYVTLQRMTISKDLGYQLEGKKEEVLL
THALNNQAPYGLIYDELGQYFTSGMDTLSAQMRSLEKMGVFSSQDHPSLARGANGEVDSLIANTRVKYFE
SIEDRKTFEILRETVGQDYYSELSGQEAEHGTFSKTVREGDLYQIREKDRVNIRELRKQTEGQGVISFQD
ALVRSAAFYIPDNEKFSSELPMRINRFIEVLPPSPATLYSIYPERKDDELAETNPDAMHFPPIKLFGYDK
AANSLLEKIEVFYELQCGAISPEEMSVCLFELFADWLDGTDTDDVLYHQEDDLFPMIEM
Protein domains
No domain identified.
Protein structure
No available structure.
T4SS
T4SS were predicted by using oriTfinder2.
Region 1: 74287..93038
| Locus tag | Coordinates | Strand | Size (bp) | Protein ID | Product | Description |
|---|---|---|---|---|---|---|
| VOI40_RS23230 (PS9952019_23650) | 69371..69682 | + | 312 | WP_011091065 | hypothetical protein | - |
| VOI40_RS23235 (PS9952019_23655) | 69815..70351 | + | 537 | WP_011091066 | hypothetical protein | - |
| VOI40_RS23240 (PS9952019_23665) | 70852..71217 | - | 366 | WP_011091067 | hypothetical protein | - |
| VOI40_RS23245 (PS9952019_23670) | 71232..71810 | + | 579 | WP_223811519 | plasmid mobilization protein MobA | - |
| VOI40_RS23250 (PS9952019_23675) | 71797..73776 | + | 1980 | WP_050868819 | TraI/MobA(P) family conjugative relaxase | - |
| VOI40_RS23255 (PS9952019_23680) | 73790..74290 | + | 501 | WP_004187320 | DotD/TraH family lipoprotein | - |
| VOI40_RS23260 (PS9952019_23685) | 74287..75066 | + | 780 | WP_004187315 | type IV secretory system conjugative DNA transfer family protein | traI |
| VOI40_RS23265 (PS9952019_23690) | 75077..76240 | + | 1164 | WP_004187313 | plasmid transfer ATPase TraJ | virB11 |
| VOI40_RS23270 (PS9952019_23695) | 76230..76490 | + | 261 | WP_004187310 | IcmT/TraK family protein | traK |
| VOI40_RS23275 (PS9952019_23700) | 76515..79727 | + | 3213 | WP_011091070 | LPD7 domain-containing protein | - |
| VOI40_RS23280 (PS9952019_23705) | 79854..80066 | - | 213 | WP_305953721 | Hha/YmoA family nucleoid-associated regulatory protein | - |
| VOI40_RS23285 (PS9952019_23710) | 80038..80448 | - | 411 | WP_004187465 | H-NS family nucleoid-associated regulatory protein | - |
| VOI40_RS23290 (PS9952019_23715) | 80516..81229 | + | 714 | WP_109914255 | DotI/IcmL family type IV secretion protein | traM |
| VOI40_RS23295 (PS9952019_23720) | 81238..82389 | + | 1152 | WP_011091074 | DotH/IcmK family type IV secretion protein | traN |
| VOI40_RS23300 (PS9952019_23725) | 82401..83750 | + | 1350 | WP_011091075 | conjugal transfer protein TraO | traO |
| VOI40_RS23305 (PS9952019_23730) | 83762..84466 | + | 705 | WP_011091076 | conjugal transfer protein TraP | traP |
| VOI40_RS23310 (PS9952019_23735) | 84490..85020 | + | 531 | WP_011091077 | conjugal transfer protein TraQ | traQ |
| VOI40_RS23315 (PS9952019_23740) | 85037..85426 | + | 390 | WP_011091078 | DUF6750 family protein | traR |
| VOI40_RS23320 (PS9952019_23745) | 85472..85966 | + | 495 | WP_011091080 | hypothetical protein | - |
| VOI40_RS23325 (PS9952019_23750) | 85963..89013 | + | 3051 | WP_011091081 | hypothetical protein | traU |
| VOI40_RS23330 (PS9952019_23755) | 89010..90218 | + | 1209 | WP_011091082 | conjugal transfer protein TraW | traW |
| VOI40_RS23335 (PS9952019_23760) | 90215..90865 | + | 651 | WP_011091083 | hypothetical protein | - |
| VOI40_RS23340 (PS9952019_23765) | 90858..93038 | + | 2181 | WP_004187492 | DotA/TraY family protein | traY |
| VOI40_RS23345 (PS9952019_23770) | 93041..93694 | + | 654 | WP_011091084 | hypothetical protein | - |
| VOI40_RS23350 (PS9952019_23775) | 93769..93999 | + | 231 | WP_011091085 | IncL/M type plasmid replication protein RepC | - |
Host bacterium
| ID | 19568 | GenBank | NZ_OY979225 |
| Plasmid name | P_PS9952019_2 | Incompatibility group | IncL/M |
| Plasmid size | 94284 bp | Coordinate of oriT [Strand] | 71386..71491 [+] |
| Host baterium | Providencia stuartii strain PS9952019BGR isolate PS9952019 |
Cargo genes
| Drug resistance gene | blaTEM-1B, aac(3)-IId, dfrA12, aadA2, qacE, sul1, armA, msr(E), mph(E), blaCTX-M-3 |
| Virulence gene | - |
| Metal resistance gene | - |
| Degradation gene | - |
| Symbiosis gene | - |
| Anti-CRISPR | - |