Detailed information of oriT

oriT

The information of the oriT region

• oriTDB ID: 119114
• Name: oriT_pCD034-3
• Organism: Lentilactobacillus buchneri subsp. silagei CD034
• Sequence Completeness: -
• NCBI accession of oriT (coordinates [strand]): NC_018611 (3333..3353 [-], 21 nt)
• oriT length: 21 nt
• IRs (inverted repeats): _
• Location of nic site: _
• Conserved sequence flanking the nic site: _
• Note: Predicted by oriTfinder 2.0

oriT sequence

• Length: 21 nt

>oriT_pCD034-3
AGTGGGGTGTAAGTGCGCATT

oriT secondary structure

Predicted by RNAfold.

Relaxase

mobQ_LBUCD034_RS12000_pCD034-3

• ID: 12073
• GenBank: WP_044502778
• Name: mobQ_LBUCD034_RS12000_pCD034-3
• UniProt ID: _
• Length: 687 a.a.
• Note: Predicted by oriTfinder 2.0

Relaxase protein sequence

• Length: 687 a.a.
• Molecular weight: 80647.45 Da
• Isoelectric Point: 9.4715

>WP_044502778.1 MobQ family relaxase [Lentilactobacillus buchneri]
MAIFHMSFSNISAGKGRSAIASAAYRSGEKLFDDQEGRHYFYARSVMPESFILTPKNAPAWASDREKLWN
EVERKDRRANSRYAKEFNVALPVELSEDEQKELLTKYVQENFVDEGMVADVAIHRDHPDNPHAHVMLTNR
PFNSDGTWGIKSKKQYILDENGNTMYTGTSKYPKSRKILMVDWDKKEKIIEWRHNWAVSVNQVLEQKNIP
DRISEKSFVEQGIADTPMQHEGINSKRHERKAFNQQVKNYRKSKAGYKNMQEKVVNRGHLDSLSKHFSFN
EKKVVKELSHELKTYISLESLDDKRRMLFNWKNSTLIKHAVGEDVTKQLLTINQQESSLKKADELLNKVV
DRTTKKLYPELDFEQTTAAERRELIKETNSEQTIFKGSELNERLMNIRDDLLVRQLLTFTKRPYVGWKIL
MQQEKEVKIELKYTLMIHDDSLESLEHVDQGLLEKYSPTEQQKITRAVKDLRTIMAVKQVIQTQYQEVLR
RAFPNGNFNELPMIKQEQAYTAVMYYDPVLKPCQAETIEQWQANPPQVFSPQEHQQGLAYLSGQLSLDQL
ENHHLQRVLKHDGTKQLFFGECKADPTIKNSQIEKIQKQLKGQQAKDDQYRKVNIGHYQPLNYKPVSPSY
YLKTAFSNVIMTALYARDEDYQRQKQERGLKETEWEMTKKQRQHQTRNRHEDWGMHL

Protein domains

Predicted by InterproScan.

• Nicking enzyme C-terminal middle helical domain (NES_C_h) (276-384)

• MobA/MobL family (MobA_MobL) (17-241)

Protein structure

No available structure.

Host bacterium

• ID: 19546
• GenBank: NC_018611
• Plasmid name: pCD034-3
• Incompatibility group: -
• Plasmid size: 56473 bp
• Coordinate of oriT [Strand]: 3333..3353 [-]
• Host baterium: Lentilactobacillus buchneri subsp. silagei CD034

Cargo genes

• Drug resistance gene: -
• Virulence gene: cpsF
• Metal resistance gene: -
• Degradation gene: -
• Symbiosis gene: -
• Anti-CRISPR: AcrIIA21