Detailed information of oriT

oriT

The information of the oriT region


oriTDB ID   119098
Name   oriT_p201702782-2 in_silico
Organism   Shigella sonnei strain 201702782
Sequence Completeness      -
NCBI accession of oriT (coordinates [strand])   NZ_OP038272 (39514..39586 [-], 73 nt)
oriT length   73 nt
IRs (inverted repeats)     _
Location of nic site      _
Conserved sequence flanking the
  nic site    		  _
Note   Predicted by oriTfinder 2.0

  oriT sequence  


Download         Length: 73 nt

>oriT_p201702782-2
GTCGGGGCAAAGCCCTGACCAGGTGGGGAATGTCTGAGTGCGCGTGCGCGGTCCGACATTCCCACATCCTGTC

Visualization of oriT structure

  oriT secondary structure

Predicted by RNAfold.

Download structure file


Relaxase

ID   12060 GenBank   WP_052984280
Name   Relaxase_OVP30_RS00260_p201702782-2 insolico UniProt ID   _
Length   903 a.a. PDB ID   
Note   Predicted by oriTfinder 2.0

  Relaxase protein sequence


Download         Length: 903 a.a.        Molecular weight: 104663.00 Da        Isoelectric Point: 7.4180

>WP_052984280.1 MULTISPECIES: relaxase/mobilization nuclease domain-containing protein [Enterobacteriaceae]
MNAIIPHKRRDGRSSFEDLIAYTSVRDDVQENELTEDSEIKSDVPHRNRFRRLVDYATRLRNEKFVSLID
VMKDGSQWVNFYGVTCFHNCNSLETAAEEMQYKADKAVFSRNDTDPVFHYILSWPAHESPRPEQLFDCVR
HTLKSLELSKHQYVAAVHTDTDNLHVHVAVNRVHPETGYINWLSWSQEKLSRACRELELKHGFAPDNGCF
VHAPGNRIVRKTALVRERRNAWRRGKKQTFREYIAQMSIAGLREEPAQDWLSLHKRLASDGLYITMQEGE
LVVKDGWDRAREGVALSSFGPSWTAEKLGRKLGEYQPVPTDIFSQVGTPGRYDPEAINVDIRPEKVAETE
SLKQYACRYFAERLPEMARNGELESCLDVHRTLAKAGLWMGIQHGHLVLHDGFDKQQTPVRADSVWPLMT
LDYMQDLDGGWQPVPKDIFTQVIPGERFRGRNLGTQAVSDYEWYRMRMGTGPQGAIKRELFSDKESLWGY
TTVQCESLIEDMIAGGNFSWQACHEMFARKGLMLQKQHHGLVIVDAFNHELTPVKASSIHPDLTLSRAEL
QAGPFEIAGADIFERVKPECRYNPELAASDEVEPGFRRDPVLRRERREARAAAREDLRARYLAWKEHWRK
PDLRYGERLREIHAACRRRKAYIRVQFRDPQLRKLHYHIAEVQRMQALIRLKESVKEERLSLIAEGKWYP
LSYRQWVEQQAVQGDRAALSQLRGWDYRDRRKDKRRTTNADRCVILCEPGGTPLYEDTGVLEARLQKDGS
VRFRDRRNGELVCVDYGDRVVFYHHQDRNELVDKLNLIAPVLFDREPGMGFEPEGSYQQFNDVFAEMVAW
HNAAGITGNGHFVISRPDVDLHRQRSEQYYHEYIRQQKSISGGNDTSYTPVQDNEWTPPSPGM

  Protein domains


Predicted by InterproScan.

Relaxase/Mobilisation nuclease domain (Relaxase)

(62-315)


  Protein structure



No available structure.




Auxiliary protein

ID   6894 GenBank   WP_001291058
Name   WP_001291058_p201702782-2 insolico UniProt ID   A0A7I0L241
Length   110 a.a. PDB ID   _
Note   Predicted by oriTfinder 2.0

  Auxiliary protein sequence


Download         Length: 110 a.a.        Molecular weight: 12730.52 Da        Isoelectric Point: 9.9656

>WP_001291058.1 MULTISPECIES: plasmid mobilization protein MobA [Enterobacteriaceae]
MSEKKTRTGSENRKRIVKFTARFTEDEAEIVREKAESSGQTVSTFIRSSSLDKVVNCRTDDRMIDEIMRL
GRLQKHLFVEGKRTGDKEYAEVLVAITEYVNALRRDLMGR

  Protein domains



No domain identified.



  Protein structure


Source ID Structure
AlphaFold DB A0A7I0L241


T4CP

ID   14123 GenBank   WP_000077663
Name   trbC_OVP30_RS00290_p201702782-2 insolico UniProt ID   _
Length   767 a.a. PDB ID   _
Note   Predicted by oriTfinder 2.0

  T4CP protein sequence


Download         Length: 767 a.a.        Molecular weight: 87012.13 Da        Isoelectric Point: 7.3008

>WP_000077663.1 MULTISPECIES: F-type conjugative transfer protein TrbC [Enterobacteriaceae]
MSQHHVNPDLIHRTAWGNPLWNALHNLNITGLCLAGSIITALIWPLALPVCLLFTLVTSVIFMLQRWRCP
LRMPMTLSLDDPSQDRKVRRSLFSFWPTLFQYEADETFPARGIFYVGYRRINDIGRELWLSMDDLTRHVM
FFATTGGGKTETTFAWLLNPLCWGRGFTFVDGKAQNDTTRTIWYLSRRFGREDDIEVINFMNGGKSRSEI
IQSGEKSRPQSNTWNPFAFSTEAFTAETMQSMLPQNVQGGEWQSRAIAMNKALVFGTKFWCVREGKTMSL
QMLREHMTLEGMAKLYCRGLDDQWPEEAIAPLRNYLQDVPGFDMSLVRTPSAWTEEPRKQHAYLSGQFSE
TFTTFAETFGDVFAADAGDIDIRDSIHSDRILIVMIPALDTSAHTTSALGRMFVTQQSMLLARDLGYRLE
GTDAQTLEVKKYKGSFPYICILDEVGAYYTDRIAVEATQVRSLEFSLIMTAQDQERIEGQTSATNTATLM
QNAGTKFAGRIVSDDKTARTVKNAAGEEARARMGSLQRHDGVMGESWVDGNQITIQMESKIDVQDLIRLN
AGEFFTVFQGDVVPSASVYIPDSEKSCDSDPVVINRYISVEAPRLERLRRLVPRTVQRRLPTPEHVSSII
GVLTAKPSRKRRKNRTEPYRIIDTFQRQLATAQTSLDLLPQYDTDIESRANELWKKAVHTINNTTREERR
VCYITLNRPEEEHSGPEDIPSVKAILNTLLPLEMLLPVPDLTASPPHKKNVAQTPSGGKQESRKKRF

  Protein domains


Predicted by InterproScan.

TraM recognition site of TraD and TraG (TraG-D_C)

(439-528)

  Protein structure



No available structure.




T4SS

T4SS were predicted by using oriTfinder2.

Region 1: 47440..86245

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
OVP30_RS00265 (p201702782-2__00052) 42730..43092 + 363 Protein_53 cell envelope integrity TolA C-terminal domain-containing protein -
OVP30_RS00270 (p201702782-2__00053) 43160..44140 + 981 WP_052990530 IS5-like element ISKpn26 family transposase -
OVP30_RS00275 44290..44361 + 72 Protein_55 tolA family protein -
OVP30_RS00280 44459..44689 - 231 WP_065406859 hypothetical protein -
OVP30_RS00285 (p201702782-2__00055) 44749..45042 - 294 WP_001402879 hypothetical protein -
OVP30_RS00290 (p201702782-2__00056) 45156..47459 - 2304 WP_000077663 F-type conjugative transfer protein TrbC -
OVP30_RS00295 (p201702782-2__00057) 47440..48564 - 1125 WP_000152381 DsbC family protein trbB
OVP30_RS00300 (p201702782-2__00058) 48561..49841 - 1281 WP_052973421 IncI1-type conjugal transfer protein TrbA trbA
OVP30_RS00560 50638..50733 + 96 WP_001363141 DinQ-like type I toxin DqlB -
OVP30_RS00310 (p201702782-2__00059) 50796..50972 - 177 WP_001054907 hypothetical protein -
OVP30_RS00315 (p201702782-2__00060) 51122..51589 - 468 WP_000598962 thermonuclease family protein -
OVP30_RS00320 (p201702782-2__00061) 51748..52371 + 624 WP_001155247 helix-turn-helix domain-containing protein -
OVP30_RS00325 (p201702782-2__00063) 52569..52784 - 216 WP_000266543 hypothetical protein -
OVP30_RS00330 (p201702782-2__00064) 52788..53156 - 369 WP_001345852 hypothetical protein -
OVP30_RS00335 (p201702782-2__00065) 53447..53689 - 243 WP_000650923 hypothetical protein -
OVP30_RS00340 (p201702782-2__00066) 54019..54171 + 153 WP_001335079 Hok/Gef family protein -
OVP30_RS00345 (p201702782-2__00067) 54309..55895 - 1587 WP_000004564 TnsA endonuclease N-terminal domain-containing protein -
OVP30_RS00350 (p201702782-2__00068) 56169..56822 - 654 WP_001401768 plasmid IncI1-type surface exclusion protein ExcA -
OVP30_RS00355 (p201702782-2__00069) 56910..59075 - 2166 WP_000691808 DotA/TraY family protein traY
OVP30_RS00360 (p201702782-2__00070) 59150..59719 - 570 WP_000650752 conjugal transfer protein TraX -
OVP30_RS00365 (p201702782-2__00071) 59716..60921 - 1206 WP_001189548 conjugal transfer protein TraW traW
OVP30_RS00370 (p201702782-2__00072) 60879..61499 - 621 WP_000286854 hypothetical protein traV
OVP30_RS00375 (p201702782-2__00073) 61499..64543 - 3045 WP_001024766 conjugal transfer protein traU
OVP30_RS00380 (p201702782-2__00074) 64838..65464 - 627 WP_000785703 hypothetical protein traT
OVP30_RS00565 (p201702782-2__00075) 65571..65822 - 252 WP_001277573 hypothetical protein -
OVP30_RS00390 (p201702782-2__00076) 65879..66277 - 399 WP_000088813 DUF6750 family protein traR
OVP30_RS00395 (p201702782-2__00077) 66324..66854 - 531 WP_000987023 conjugal transfer protein TraQ traQ
OVP30_RS00400 (p201702782-2__00078) 66851..67564 - 714 WP_000787003 conjugal transfer protein TraP traP
OVP30_RS00405 (p201702782-2__00079) 67561..68892 - 1332 WP_001288254 conjugal transfer protein TraO traO
OVP30_RS00410 (p201702782-2__00080) 68896..69870 - 975 WP_000547383 DotH/IcmK family type IV secretion protein traN
OVP30_RS00415 (p201702782-2__00081) 69881..70576 - 696 WP_001287545 DotI/IcmL family type IV secretion protein traM
OVP30_RS00420 (p201702782-2__00082) 70588..70938 - 351 WP_001056367 hypothetical protein traL
OVP30_RS00425 (p201702782-2__00083) 70955..75016 - 4062 WP_000842447 LPD7 domain-containing protein -
OVP30_RS00430 (p201702782-2__00084) 75080..75370 - 291 WP_000817801 IcmT/TraK family protein traK
OVP30_RS00435 (p201702782-2__00085) 75367..76515 - 1149 WP_000775210 plasmid transfer ATPase TraJ virB11
OVP30_RS00440 (p201702782-2__00086) 76499..77335 - 837 WP_000745049 type IV secretory system conjugative DNA transfer family protein traI
OVP30_RS00445 (p201702782-2__00087) 77332..77790 - 459 WP_001170111 DotD/TraH family lipoprotein -
OVP30_RS00450 (p201702782-2__00088) 77894..79096 - 1203 WP_000979993 conjugal transfer protein TraF -
OVP30_RS00455 (p201702782-2__00089) 79198..80019 - 822 WP_000845373 hypothetical protein traE
OVP30_RS00460 80184..80378 - 195 Protein_92 integrase -
OVP30_RS00465 (p201702782-2__00090) 80492..81853 - 1362 WP_001168171 shufflon system plasmid conjugative transfer pilus tip adhesin PilV -
OVP30_RS00470 (p201702782-2__00091) 81871..82407 - 537 WP_023649457 prepilin peptidase -
OVP30_RS00475 (p201702782-2__00092) 82513..82998 - 486 WP_063612151 lytic transglycosylase domain-containing protein virB1
OVP30_RS00480 (p201702782-2__00093) 83043..83579 - 537 WP_000111534 type 4 pilus major pilin -
OVP30_RS00485 (p201702782-2__00094) 83641..84735 - 1095 WP_000697158 type II secretion system F family protein -
OVP30_RS00490 (p201702782-2__00095) 84737..86245 - 1509 WP_000880807 GspE/PulE family protein virB11
OVP30_RS00495 (p201702782-2__00096) 86348..86806 - 459 WP_000095885 type IV pilus biogenesis protein PilP -
OVP30_RS00500 (p201702782-2__00097) 86796..88091 - 1296 WP_000129883 type 4b pilus protein PilO2 -
OVP30_RS00505 (p201702782-2__00098) 88112..89731 - 1620 WP_001035562 PilN family type IVB pilus formation outer membrane protein -
OVP30_RS00510 (p201702782-2__00099) 89763..90176 - 414 WP_124983997 type IV pilus biogenesis protein PilM -

Host bacterium

ID   19530 GenBank   NZ_OP038272
Plasmid name   p201702782-2 Incompatibility group   IncB/O/K/Z
Plasmid size   97029 bp Coordinate of oriT [Strand]   39514..39586 [-]
Host baterium   Shigella sonnei strain 201702782

Cargo genes

Drug resistance gene   mph(A), blaCTX-M-55, aac(3)-IIa
Virulence gene   -
Metal resistance gene   -
Degradation gene   -
Symbiosis gene   -
Anti-CRISPR   -