Detailed information of oriT

oriT

The information of the oriT region

• oriTDB ID: 119048
• Name: oriT_pCM-BC1-1
• Organism: Pediococcus inopinatus strain BC1
• Sequence Completeness: -
• NCBI accession of oriT (coordinates [strand]): NZ_CP104769 (45773..45793 [+], 21 nt)
• oriT length: 21 nt
• IRs (inverted repeats): _
• Location of nic site: _
• Conserved sequence flanking the nic site: _
• Note: Predicted by oriTfinder 2.0

oriT sequence

• Length: 21 nt

>oriT_pCM-BC1-1
AGTGGGGTGTAAGTGCGCATT

oriT secondary structure

Predicted by RNAfold.

Relaxase

mobQ_N6G94_RS10125_pCM-BC1-1

• ID: 12033
• GenBank: WP_323715381
• Name: mobQ_N6G94_RS10125_pCM-BC1-1
• UniProt ID: _
• Length: 687 a.a.
• Note: Predicted by oriTfinder 2.0

Relaxase protein sequence

• Length: 687 a.a.
• Molecular weight: 80493.14 Da
• Isoelectric Point: 9.5200

>WP_323715381.1 MobQ family relaxase [Pediococcus inopinatus]
MAIFHMSFSNISAGKGRSAIASAAYRSGEKLFDDQEGRHYFYARSVIPESFILTPKNAPAWASDREKLWN
EVERKDRRANSRYAKEFNVALPVELSEDEQKELLTKYVQENFVDEGMVADVAIHRDHPDNPHAHVMLTNR
PFNSDGTWGIKSKKQYILDDNGDKTYTGTSKYPKSRKILMVDWDKKEKITEWRHNWAASVNQALEQKNIP
DRISEKSFVEQGIADTPMQHEGINSKRSKRKEFNQQVKKYRKTKANYKNNQEKVINRGHLDSLSKHFSFN
EKKVVKELSHELKTYISLESLDDKRRMLFNWKNSTLIKHAFGEDITKQLLTINQQESSLKKADELLNKVV
DRTTKKLYPELDFEQTTAAERRELIKETNSEQTIFKGSELNERLMNIRDDLLVRQLLTFTKRPYVGWKLL
MQQEKEVKIELKYTLMIHDDNLESLEHVDQGLLEKYSPTEQQKITRAVKDLRTIMAVKQVIQTQYQEVLR
RAFPNGNFNELPMIKQEQAYTAVMYYDPVLKPCQAETIEQWQANPPQVFSPQEHQQGLAYLSGQLSLDQL
ENHHLQRVLKHDGTKQLFFGECKADPTIKNSQIEKIQKQLKGQQAKDDQYRKVNIGHYQPLNYKPVSPSY
YLKTAFSNAIMTALYARDEDYERQKQAQGLKEIEWEMTKKQRQHQTRNRHEDGGMHL

Protein domains

Predicted by InterproScan.

• MobA/MobL family (MobA_MobL) (17-241)

• Nicking enzyme C-terminal middle helical domain (NES_C_h) (276-384)

Protein structure

No available structure.

Host bacterium

• ID: 19480
• GenBank: NZ_CP104769
• Plasmid name: pCM-BC1-1
• Incompatibility group: -
• Plasmid size: 49816 bp
• Coordinate of oriT [Strand]: 45773..45793 [+]
• Host baterium: Pediococcus inopinatus strain BC1

Cargo genes

• Drug resistance gene: -
• Virulence gene: -
• Metal resistance gene: -
• Degradation gene: -
• Symbiosis gene: -
• Anti-CRISPR: -