Detailed information of oriT

oriT

The information of the oriT region

• oriTDB ID: 119005
• Name: oriT_Dog026|unnamed3
• Organism: Mammaliicoccus lentus strain Dog026
• Sequence Completeness: -
• NCBI accession of oriT (coordinates [strand]): NZ_CP120158 (4231..4268 [-], 38 nt)
• oriT length: 38 nt
• IRs (inverted repeats): 14..22, 29..37 (TAAAGTATA..TATACTTTA) 2..8, 13..19 (ACTTTAT..ATAAAGT)
• Location of nic site: 27..28
• Conserved sequence flanking the nic site: GTGTGTTATA
• Note: Predicted by oriTfinder 2.0

oriT sequence

• Length: 38 nt

>oriT_Dog026|unnamed3
CACTTTATGAATATAAAGTATAGTGTGTTATACTTTAC

oriT secondary structure

Predicted by RNAfold.

Relaxase

mobV_P3U54_RS14425_Dog026|unnamed3

• ID: 12030
• GenBank: WP_323711121
• Name: mobV_P3U54_RS14425_Dog026|unnamed3
• UniProt ID: _
• Length: 418 a.a.
• Note: Predicted by oriTfinder 2.0

Relaxase protein sequence

• Length: 418 a.a.
• Molecular weight: 49431.13 Da
• Isoelectric Point: 9.9136

>WP_323711121.1 MobV family relaxase [Mammaliicoccus lentus]
MSYAVCRMQKVKSAGLKGMQFHNQRERKSRTNDDIDHERTRENYDLKNDKNIDYNERVKEIIESQKTGTR
KTRKDAVLVNELLVTSDRDFFEQLDPGEQKRFFEESYKLFSERYGKQNIAYATVHNDEQTPHMHLGVVPM
RDGKLQGKNVFNRQELLWLQDKFPEHMKKQGFELKRGERGSDRKHIETAKFKKQTLEKEIDFLEKNLAVK
KDEWTAYSDKVKSDLEVPAKRHMKSVEVPTGEKSMFGLGKEIMKTEKKPTKNVVISERDYKNLVTAARDN
DRLKQHVRNLMSTDMAREYKKLSKEHGQVKEKYSGLVERFNENVNDYNELLEENKSLKSKISDLKRDVSL
IYESTKEFLKERTDGLKAFKNVFKGFVDKVKDKTAQFQEKHDLEPKKNEFEKIHSKELNKEKDRGMEL

Protein domains

Predicted by InterproScan.

• Plasmid recombination enzyme (Mob_Pre) (1-193)

Protein structure

No available structure.

Host bacterium

• ID: 19437
• GenBank: NZ_CP120158
• Plasmid name: Dog026|unnamed3
• Incompatibility group: -
• Plasmid size: 6274 bp
• Coordinate of oriT [Strand]: 4231..4268 [-]
• Host baterium: Mammaliicoccus lentus strain Dog026

Cargo genes

• Drug resistance gene: tet(L), dfrK
• Virulence gene: -
• Metal resistance gene: -
• Degradation gene: -
• Symbiosis gene: -
• Anti-CRISPR: -