Detailed information of oriT

oriT

The information of the oriT region

• oriTDB ID: 118974
• Name: oriT_pV1344-optrA
• Organism: Enterococcus faecalis strain V1344
• Sequence Completeness: -
• NCBI accession of oriT (coordinates [strand]): NZ_OR824990 (47659..47708 [+], 50 nt)
• oriT length: 50 nt
• IRs (inverted repeats): _
• Location of nic site: 34..35
• Conserved sequence flanking the nic site: GCTTGCAAAA
• Note: Predicted by oriTfinder 2.0

oriT sequence

• Length: 50 nt

>oriT_pV1344-optrA
AATATCGCAACATGCTAGCATGTTGCTCCGCTTGCAAAAAGAAAGCCTAC

oriT secondary structure

Predicted by RNAfold.

Relaxase

Relaxase_VHB24_RS00240_pV1344-optrA

• ID: 12023
• GenBank: WP_010784826
• Name: Relaxase_VHB24_RS00240_pV1344-optrA
• UniProt ID: A0A8E7CAC2
• Length: 561 a.a.
• Note: Predicted by oriTfinder 2.0

Relaxase protein sequence

• Length: 561 a.a.
• Molecular weight: 65352.94 Da
• Isoelectric Point: 6.0135

>WP_010784826.1 MULTISPECIES: relaxase/mobilization nuclease domain-containing protein [Enterococcus]
MVYTKHFVIHTFDKLNNACSYIENAEKTEVTNDNPSEHLEHLFQYIVNDDKTYMKKLVSGHGIVDPTNPY
EEFKLTKLQAAIQRKIGYTFDPKSERLLPPTLTELEKGNAVLAHHLIQSFSPEDDLTPEKIHEIGYNTVM
ELTGGEYEFVIATHVDKEHLHNHIIFSSTNLKTGKAFRWQKGTKKVFEQISDKIAAKEGAKIIEKSPKQT
HTKYTKWQTESIYKQKIKSRLDFLLEQSSSINDFLEKAEALNLSADFSNKWTTYRLLDEPQIKNTRSRSL
SKSDPTRYNYEKIIERLKENKNVLTVEECVKRYEEKNEQEKNNFDYQFTIEPWQLSHKTERGYYINIDYG
YGNSGKLFVGGYKVDPLDNGNYNIYIKRNEYFYFMSDKDATKSKYLTGASLIKQLRLYNGQTPLKREPVM
QTIDELVSAINFLAANEIEDTRQLKLLEEKLEVAFLEAEKTLETLDEKMLELHQLSNLLLENELQGDPEL
IQKKLKTLLPDATLAEFSYEDVRGEIEAIKTSQTLLESKLERTRNEINQLHEIQAVQEKEPEKQTDIKPK
L

Protein domains

Predicted by InterproScan.

• Relaxase/Mobilisation nuclease domain (Relaxase) (37-296)

Protein structure

No available structure.

Auxiliary protein

WP_002362408_pV1344-optrA

• ID: 6869
• GenBank: WP_002362408
• Name: WP_002362408_pV1344-optrA
• UniProt ID: A0A1J6XY92
• Length: 118 a.a.
• PDB ID: _
• Note: Predicted by oriTfinder 2.0

Auxiliary protein sequence

• Length: 118 a.a.
• Molecular weight: 13979.04 Da
• Isoelectric Point: 9.6545

>WP_002362408.1 MULTISPECIES: plasmid mobilization relaxosome protein MobC [Bacillota]
MENKQKLKRPIQRIVRLSEEENNLIKRKIEESFFPNFQNFALHLLIQGEIRHVDYSELNRLTTEIHKIGI
NINQMARLANQFHEISSEDIKDLTDKVQSLNALVQSELNKLIKRKEQS

Protein domains

Predicted by InterproScan.

• Bacterial mobilisation protein (MobC) (MobC) (64-105)

Protein structure

• AlphaFold DB: A0A1J6XY92

T4CP

t4cp2_VHB24_RS00220_pV1344-optrA

• ID: 14089
• GenBank: WP_174096927
• Name: t4cp2_VHB24_RS00220_pV1344-optrA
• UniProt ID: _
• Length: 609 a.a.
• PDB ID: _
• Note: Predicted by oriTfinder 2.0

T4CP protein sequence

• Length: 609 a.a.
• Molecular weight: 70173.19 Da
• Isoelectric Point: 6.1807

>WP_174096927.1 VirD4-like conjugal transfer protein, CD1115 family [Enterococcus faecalis]
MQTYKKELKPYLYLGVFLFVIGFGIGNFFWLLPGTDYFSKTNYLFTKDILTYIEQTFYRFFITPSLLGFS
VGILGFLLGLLMYVRDNDRGIYRHGEEYGSARFATPAEMKKYEDPIPENNIIISKHVKISLFNKRLPIKL
QKNKNIAILGDSGAAKTLAFIKTNLMQRHASFITTDPDGGILPEIGLLLKKGKYKIKVLDLNTLSNSDTF
NVFEYMHSELDVDRVLEAVTEATKEVEKTTGDDFWIKAEGLLVRSLIAYLWFDGRRNDYTPHLGMIADML
RHLKRKDKNVPSPVEEWFEELNEAIPDNYAYRQWTLFNNLYESETRASVLGIAAARYSVFDHEEVVNMIR
TDTMDIDSWNEEKTAVFIAIPETNTSFNFIAALMFATVGEVLRNKSDQVRLGKRVLEEGKELLHVRFLID
EFANIGRIPHFEKMLTTFRKREMSFTIVLQSLNQLQTLYRNGWQNILNGCACLLYLGGDEEETTKYLSAR
AGKQTLSIRKHSMNKGNQGGGSENRDKYGRDLIDRSEVALIGGDECLVFISKEHVFKDKKFYAFQHDRAD
ELATGPTDDKWYTWRRYMTDEEAILDKISATDIIDHGEITSELKEETHV

Protein domains

Predicted by InterproScan.

• Type IV secretory system Conjugative DNA transfer (T4SS-DNA_transf) (144-558)

Protein structure

No available structure.

T4SS

T4SS were predicted by using oriTfinder2.

Region 1: 27731..45015

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
VHB24_RS00125	23593..23973	+	381	WP_002369738	hypothetical protein	-
VHB24_RS00130	24024..24245	+	222	WP_002406556	hypothetical protein	-
VHB24_RS00135	24547..24852	+	306	WP_010715949	hypothetical protein	-
VHB24_RS00140	24863..27538	+	2676	WP_323145172	surface exclusion protein SEA1/PrgA	-
VHB24_RS00145	27731..31648	+	3918	WP_323145171	LPXTG-anchored aggregation substance PrgB/Asc10	prgB
VHB24_RS00150	31710..32063	+	354	WP_002367913	pheromone response system RNA-binding regulator PrgU	-
VHB24_RS00155	32091..32948	+	858	WP_002367914	pheromone response LPXTG-anchored adhesin PrgC	prgC
VHB24_RS00160	32990..33889	+	900	WP_002367915	hypothetical protein	-
VHB24_RS00165	33909..34343	+	435	WP_002367916	hypothetical protein	-
VHB24_RS00170	34390..34617	+	228	WP_002365858	hypothetical protein	-
VHB24_RS00175	34631..34927	+	297	WP_002387740	hypothetical protein	-
VHB24_RS00180	34942..35742	+	801	WP_002393746	hypothetical protein	prgHb
VHB24_RS00185	35744..36097	+	354	WP_002367919	PrgI family mobile element protein	prgIc
VHB24_RS00190	36051..38441	+	2391	WP_002365863	VirB4-like conjugal transfer ATPase, CD1110 family	virb4
VHB24_RS00195	38453..41068	+	2616	WP_136498887	phage tail tip lysozyme	prgK
VHB24_RS00200	41092..41718	+	627	WP_002399631	hypothetical protein	prgL
VHB24_RS00205	41696..41950	+	255	WP_080005582	type III secretion system protein PrgM	-
VHB24_RS00210	41934..42542	+	609	WP_010831028	hypothetical protein	-
VHB24_RS00215	42701..43186	+	486	WP_002386493	PcfB family protein	gbs1365
VHB24_RS00220	43186..45015	+	1830	WP_174096927	VirD4-like conjugal transfer protein, CD1115 family	virb4
VHB24_RS00225	45065..47224	+	2160	WP_029888121	ArdC-like ssDNA-binding domain-containing protein	-
VHB24_RS00230	47257..47529	+	273	WP_029888122	LtrD	-
VHB24_RS00235	47775..48131	+	357	WP_002362408	plasmid mobilization relaxosome protein MobC	-
VHB24_RS00240	48132..49817	+	1686	WP_010784826	relaxase/mobilization nuclease domain-containing protein	-

Host bacterium

• ID: 19406
• GenBank: NZ_OR824990
• Plasmid name: pV1344-optrA
• Incompatibility group: -
• Plasmid size: 67374 bp
• Coordinate of oriT [Strand]: 47659..47708 [+]
• Host baterium: Enterococcus faecalis strain V1344

Cargo genes

• Drug resistance gene: fexA, optrA, erm(A)
• Virulence gene: prgB/asc10
• Metal resistance gene: -
• Degradation gene: -
• Symbiosis gene: -
• Anti-CRISPR: AcrIIA21, AcrIIA9