Detailed information of oriT
oriT
The information of the oriT region
| oriTDB ID | 118960 |
| Name | oriT_pSymB  |
| Organism | Sinorhizobium medicae strain ml49 |
| Sequence Completeness | - |
| NCBI accession of oriT (coordinates [strand]) | NZ_CP140897 (669967..670000 [-], 34 nt) |
| oriT length | 34 nt |
| IRs (inverted repeats) | 20..25, 29..34 (CGTCGC..GCGACG) |
| Location of nic site | _ |
| Conserved sequence flanking the nic site |
_ |
| Note | Predicted by oriTfinder 2.0 |
oriT sequence
Download Length: 34 nt
>oriT_pSymB
ATCCAAGGGCGCAATTATACGTCGCAGGGCGACG
ATCCAAGGGCGCAATTATACGTCGCAGGGCGACG
Visualization of oriT structure
oriT secondary structure
Predicted by RNAfold.
Download structure file
Relaxase
| ID | 12016 | GenBank | WP_322887484 |
| Name | traA_U8C36_RS30825_pSymB |
UniProt ID | _ |
| Length | 1102 a.a. | PDB ID | |
| Note | Predicted by oriTfinder 2.0 | ||
Relaxase protein sequence
Download Length: 1102 a.a. Molecular weight: 122620.62 Da Isoelectric Point: 10.1964
>WP_322887484.1 Ti-type conjugative transfer relaxase TraA [Sinorhizobium medicae]
MAVPHFSVSLITRGSGRSAVLSAAYRHCAKMEYQREARTIDYTRKTGLLHEEFVIPDNAPEWLQSMITDR
SVSDAAEAFWNKVEDFEKRSDAQLAKEVTIALPIELTSAQNIALVRDFVEQHISAKGMVADWVYHDAPGN
PHVHLMTTLRPLTQQGFGAKKVAVLGPDGYPIRNDAGKIVYELWAGSLDDFNALRDGWFACQNRHLTLAG
LDIRIDGRSFEKQGIELAPTLHLGAGTKAIERKATAEAKTLSLERLQLQEDLRSENARRLQRRPEIVLDL
ITRERSVFDERDVAKILHRYVDDPAVFRSLMARILQSPETLQLERERTAFATGIRAPAKYTTRALIRLEA
EMANRAIWLSRQSSHRLREAVLEVTLARHSRLSDEQKTAIEHVAKAGRIAAVIGRAGAGKTTMMKAAREA
WEAAGYRVVGGALAGKAAEGLEKEAGIISRTLSAWELRWSQGRNQLDARTVFILDEAGMVASRQMACFIE
AMTRTGAKLVLVGDPEQLQPIEAGAAFRAIADRIGYAELETIYRQREQWMRDASLDLARGNVGKAVEAYR
ANGRMIGAELKAEAVRNLVADWNRDYDPTKTTLILAHLRRDVRMLNEMARSKLVERGIVREGFAFRTADG
NRNFAAGDQIVFLKNEGSLGVKNGMLGKIVEASPNRVVAEIGAGEHHRQVIIEQRFYNNLDHGYATTIHK
SQGATVDRVKVLASLSLDRHLVYVAMTRHRDNLDVYYGARSFARAGGLIQVLSRKNSKETTLDYEKATFY
RQALRFAEARGLHLLNVARTIARDRLAWTARQSSRLADLAHRLVRIGRKLGLAAGTNPINSQNSKETRPM
VAGIATHPESVEQAVADKLAADPGLKKQWDDVSTRFHLVYAHPAAAFKAVNIDVMLKDETAAKATLSKIA
NQPDSYGALKGKTGLLASRADRQDRERAVRNVPALVQSLDGYMRQRGSAERRYRAEELEARRKVAIDIPA
LSPGARQILERVRDAIDRNDLSAALEFARADRHVKAELDGFANAVDARFGKRTFLPLSARDTNGDTFTSV
TAGMNPGQRLEVESAWKAMRTVQQLSDHERTTEALKHSETLRLSKSQGLSLG
MAVPHFSVSLITRGSGRSAVLSAAYRHCAKMEYQREARTIDYTRKTGLLHEEFVIPDNAPEWLQSMITDR
SVSDAAEAFWNKVEDFEKRSDAQLAKEVTIALPIELTSAQNIALVRDFVEQHISAKGMVADWVYHDAPGN
PHVHLMTTLRPLTQQGFGAKKVAVLGPDGYPIRNDAGKIVYELWAGSLDDFNALRDGWFACQNRHLTLAG
LDIRIDGRSFEKQGIELAPTLHLGAGTKAIERKATAEAKTLSLERLQLQEDLRSENARRLQRRPEIVLDL
ITRERSVFDERDVAKILHRYVDDPAVFRSLMARILQSPETLQLERERTAFATGIRAPAKYTTRALIRLEA
EMANRAIWLSRQSSHRLREAVLEVTLARHSRLSDEQKTAIEHVAKAGRIAAVIGRAGAGKTTMMKAAREA
WEAAGYRVVGGALAGKAAEGLEKEAGIISRTLSAWELRWSQGRNQLDARTVFILDEAGMVASRQMACFIE
AMTRTGAKLVLVGDPEQLQPIEAGAAFRAIADRIGYAELETIYRQREQWMRDASLDLARGNVGKAVEAYR
ANGRMIGAELKAEAVRNLVADWNRDYDPTKTTLILAHLRRDVRMLNEMARSKLVERGIVREGFAFRTADG
NRNFAAGDQIVFLKNEGSLGVKNGMLGKIVEASPNRVVAEIGAGEHHRQVIIEQRFYNNLDHGYATTIHK
SQGATVDRVKVLASLSLDRHLVYVAMTRHRDNLDVYYGARSFARAGGLIQVLSRKNSKETTLDYEKATFY
RQALRFAEARGLHLLNVARTIARDRLAWTARQSSRLADLAHRLVRIGRKLGLAAGTNPINSQNSKETRPM
VAGIATHPESVEQAVADKLAADPGLKKQWDDVSTRFHLVYAHPAAAFKAVNIDVMLKDETAAKATLSKIA
NQPDSYGALKGKTGLLASRADRQDRERAVRNVPALVQSLDGYMRQRGSAERRYRAEELEARRKVAIDIPA
LSPGARQILERVRDAIDRNDLSAALEFARADRHVKAELDGFANAVDARFGKRTFLPLSARDTNGDTFTSV
TAGMNPGQRLEVESAWKAMRTVQQLSDHERTTEALKHSETLRLSKSQGLSLG
Protein domains
Predicted by InterproScan.
Protein structure
No available structure.
T4CP
| ID | 14075 | GenBank | WP_322887421 |
| Name | tcpA_U8C36_RS35185_pSymB |
UniProt ID | _ |
| Length | 951 a.a. | PDB ID | _ |
| Note | Predicted by oriTfinder 2.0 | ||
T4CP protein sequence
Download Length: 951 a.a. Molecular weight: 102081.58 Da Isoelectric Point: 4.5063
>WP_322887421.1 DNA translocase FtsK [Sinorhizobium medicae]
MRIPRTNFSTAALYDANQAEDFEDPQPVVPGIVHASAPESAAHDHLFEAPEAPRRASGYRQEEISQATRA
VRLYGHGSAYAPAEVTVSTRDPLPTLAEITGLSCEPGWESHFFLSPNVRFTRTPEREFMKRRPPVPEDNE
VDVAEAAAEAPLAETVTGKEPVEPLPSPAETDAPSYSPSELLRVLVQQLPSFNAAHLQTPEKNAVEQAAE
AAVLTEEEPSIPQAPHVPIMEEAPVVADASTGTAAVPDSAGAEDVARQSEARLSYLSDFAFFEFMPLEPP
VALRAVAAPAEESAHVAAPSSALPKAAAPNIVSAPVRIPSQPPAAITSLFRVVECRRPEAGPDVSAPDAP
QDLGAEPISAGAAVGAEAVEAQGLTPEAPDEPAREPAPEAAVVPAKEPAPEAPVTRAAITMPAVIQRSSP
ALPPVGATERPGIADAYEFPSKELLQEPPQGQGFFMTQEQLEQNAGLLESVLEDFGVKGEIIHVRPGPVV
TLYEFEPAPGVKSSRVIGLADDIARSMSALSARVAVVPGRNVIGIELPNATRETVYFRELIESGDFQKTG
CKLALCLGKTIGGEPVIAELAKMPHLLVAGTTGSGKSVAINTMILSLLYRLKPEECRLIMVDPKMLELSV
YDGIPHLLTPVVTDPKKAVMALKWAVREMEDRYRKMSRLGVRNIDGYNQRAAAAREKGEPILATVQTGFE
KGTGEPLFEQQEMDLSPMPYIVVIVDEMADLMMVAGKEIEGAIQRLAQMARAAGIHLIMATQRPSVDVIT
GTIKANFPTRISFQVTSKIDSRTILGEQGAEQLLGQGDMLHMAGGGRIARVHGPFVSDQEVEHVVAHLKT
QGRPEYLETVTADEEEEEPEEDQGAVFDKSAIAAEDGNELYDQAVKVVLRDKKCSTSYIQRRLGIGYNRA
ASLVERMEKDGLVGPANHVGKREIIYGNRDGGPKPDSDELD
MRIPRTNFSTAALYDANQAEDFEDPQPVVPGIVHASAPESAAHDHLFEAPEAPRRASGYRQEEISQATRA
VRLYGHGSAYAPAEVTVSTRDPLPTLAEITGLSCEPGWESHFFLSPNVRFTRTPEREFMKRRPPVPEDNE
VDVAEAAAEAPLAETVTGKEPVEPLPSPAETDAPSYSPSELLRVLVQQLPSFNAAHLQTPEKNAVEQAAE
AAVLTEEEPSIPQAPHVPIMEEAPVVADASTGTAAVPDSAGAEDVARQSEARLSYLSDFAFFEFMPLEPP
VALRAVAAPAEESAHVAAPSSALPKAAAPNIVSAPVRIPSQPPAAITSLFRVVECRRPEAGPDVSAPDAP
QDLGAEPISAGAAVGAEAVEAQGLTPEAPDEPAREPAPEAAVVPAKEPAPEAPVTRAAITMPAVIQRSSP
ALPPVGATERPGIADAYEFPSKELLQEPPQGQGFFMTQEQLEQNAGLLESVLEDFGVKGEIIHVRPGPVV
TLYEFEPAPGVKSSRVIGLADDIARSMSALSARVAVVPGRNVIGIELPNATRETVYFRELIESGDFQKTG
CKLALCLGKTIGGEPVIAELAKMPHLLVAGTTGSGKSVAINTMILSLLYRLKPEECRLIMVDPKMLELSV
YDGIPHLLTPVVTDPKKAVMALKWAVREMEDRYRKMSRLGVRNIDGYNQRAAAAREKGEPILATVQTGFE
KGTGEPLFEQQEMDLSPMPYIVVIVDEMADLMMVAGKEIEGAIQRLAQMARAAGIHLIMATQRPSVDVIT
GTIKANFPTRISFQVTSKIDSRTILGEQGAEQLLGQGDMLHMAGGGRIARVHGPFVSDQEVEHVVAHLKT
QGRPEYLETVTADEEEEEPEEDQGAVFDKSAIAAEDGNELYDQAVKVVLRDKKCSTSYIQRRLGIGYNRA
ASLVERMEKDGLVGPANHVGKREIIYGNRDGGPKPDSDELD
Protein domains
Predicted by InterproScan.
Protein structure
No available structure.
Host bacterium
| ID | 19392 | GenBank | NZ_CP140897 |
| Plasmid name | pSymB | Incompatibility group | - |
| Plasmid size | 1592858 bp | Coordinate of oriT [Strand] | 669967..670000 [-] |
| Host baterium | Sinorhizobium medicae strain ml49 |
Cargo genes
| Drug resistance gene | - |
| Virulence gene | - |
| Metal resistance gene | actP |
| Degradation gene | - |
| Symbiosis gene | - |
| Anti-CRISPR | AcrVIB, AcrIIA7, AcrIIA9 |