Detailed information of oriT
oriT
The information of the oriT region
| oriTDB ID | 118948 |
| Name | oriT_pAt15-1187-1-2a2  |
| Organism | Agrobacterium tumefaciens strain 15-1187-1-2a |
| Sequence Completeness | - |
| NCBI accession of oriT (coordinates [strand]) | NZ_CP123841 (67755..67798 [+], 44 nt) |
| oriT length | 44 nt |
| IRs (inverted repeats) | 21..27, 32..38 (ACGTCGC..GCGACGT) |
| Location of nic site | _ |
| Conserved sequence flanking the nic site |
_ |
| Note | Predicted by oriTfinder 2.0 |
oriT sequence
Download Length: 44 nt
>oriT_pAt15-1187-1-2a2
GGATCCAAGGGCGCAATTATACGTCGCTGATGCGACGTGCTTGC
GGATCCAAGGGCGCAATTATACGTCGCTGATGCGACGTGCTTGC
Visualization of oriT structure
oriT secondary structure
Predicted by RNAfold.
Download structure file
Relaxase
| ID | 12008 | GenBank | WP_065657879 |
| Name | traA_A6U93_RS27335_pAt15-1187-1-2a2 |
UniProt ID | A0AA44F421 |
| Length | 1100 a.a. | PDB ID | |
| Note | Predicted by oriTfinder 2.0 | ||
Relaxase protein sequence
Download Length: 1100 a.a. Molecular weight: 123610.15 Da Isoelectric Point: 9.7013
>WP_065657879.1 Ti-type conjugative transfer relaxase TraA [Agrobacterium tumefaciens]
MAIAHFSASIVSRGGGRSAVLSAAYRHCAKMEYEREARTIDYTRKQGLLHEEFVLPADAPKWVRAMIADR
SVAGASEAFWNKVEAFEKRSDAQLARDLTIALPLELSAEQNIALVRDFVDKHILAKGMVADWVYHDNPGN
PHIHLMTTLRPLTEDGFGSKKVAVIGQDGQPVRTKSGKILYELWAGSTDDFNSLRDGWFERLNHHLALGG
IDLRIDGRSYEKQGIELEPTIHLGVGAKAIERKAQEQGVRPELERIELNEERRSENTRRILNNPAIVLDL
ITREKSVFDERDVAKVLHRYVDDPTVFQQLMLRIIQNPEVLRLQRDTIEFATGEKVPARYSTRVMIRLEA
TMVRQALWLSNRDGHAVSEAALAATFRRHERLSEEQKTAIERIAGPARIGAVVGRAGAGKTTMMKAAREV
WELAGYRVVGGALSGKAAEGLEKEAGIQSRTLASWELRWNRGRDVLDDRTVFVMDEAGMVASKQMAGFVD
AVVRAGAKIVLVGDPEQLQPIEAGAAFRAIVARVGYAELETIYRQREDWMRKASLDLARGNVEKALTAYD
VNLRITGTRLKAEAVERLIGDWNHDYDQTKTTLILAHLRRDVRMLNVMAREKLVERGIVGEGHVFKTADG
IRQFDVGDQIVFLKNEASLGVKNGMIAHVIEAAPSRIVAVIGEGDQRRQVIVEQRFYSNLDHGYATTIHK
SQGATVDRVKVLASLSLDRHLTYVAMTRHREDLQLYYGHRSFAFNGGLAKVLSRRQAKETTLDYERGKLY
REALRYAENRGLNIVQVARTLLRDQLDWTLRQKTKLVDLGQRLAGFAERLGLHHPLKTQTTKEAAPMVAG
IKTFSGSIADTVENKLDTDPALRQQWDEVSARFAYVFADPETAFRAMNFDAVLVDKETAKLALQTLTTAP
ASIGPLKGKTGILASKTEREDRRVADLNVPALKRDLEQYLRMRETAVQRLQADEQVLRQRVSIDIPALSP
AAHVVLERVRDAIDRNDLPAAMAYALSNRETKLEIDGFNQAVTERFGERTLLTNAAREPSGKLFDKLSEG
MQPEQKERLKEAWPIMRTSQQLAAHERTVQTLRQAEDLRLAQRQTPVMKQ
MAIAHFSASIVSRGGGRSAVLSAAYRHCAKMEYEREARTIDYTRKQGLLHEEFVLPADAPKWVRAMIADR
SVAGASEAFWNKVEAFEKRSDAQLARDLTIALPLELSAEQNIALVRDFVDKHILAKGMVADWVYHDNPGN
PHIHLMTTLRPLTEDGFGSKKVAVIGQDGQPVRTKSGKILYELWAGSTDDFNSLRDGWFERLNHHLALGG
IDLRIDGRSYEKQGIELEPTIHLGVGAKAIERKAQEQGVRPELERIELNEERRSENTRRILNNPAIVLDL
ITREKSVFDERDVAKVLHRYVDDPTVFQQLMLRIIQNPEVLRLQRDTIEFATGEKVPARYSTRVMIRLEA
TMVRQALWLSNRDGHAVSEAALAATFRRHERLSEEQKTAIERIAGPARIGAVVGRAGAGKTTMMKAAREV
WELAGYRVVGGALSGKAAEGLEKEAGIQSRTLASWELRWNRGRDVLDDRTVFVMDEAGMVASKQMAGFVD
AVVRAGAKIVLVGDPEQLQPIEAGAAFRAIVARVGYAELETIYRQREDWMRKASLDLARGNVEKALTAYD
VNLRITGTRLKAEAVERLIGDWNHDYDQTKTTLILAHLRRDVRMLNVMAREKLVERGIVGEGHVFKTADG
IRQFDVGDQIVFLKNEASLGVKNGMIAHVIEAAPSRIVAVIGEGDQRRQVIVEQRFYSNLDHGYATTIHK
SQGATVDRVKVLASLSLDRHLTYVAMTRHREDLQLYYGHRSFAFNGGLAKVLSRRQAKETTLDYERGKLY
REALRYAENRGLNIVQVARTLLRDQLDWTLRQKTKLVDLGQRLAGFAERLGLHHPLKTQTTKEAAPMVAG
IKTFSGSIADTVENKLDTDPALRQQWDEVSARFAYVFADPETAFRAMNFDAVLVDKETAKLALQTLTTAP
ASIGPLKGKTGILASKTEREDRRVADLNVPALKRDLEQYLRMRETAVQRLQADEQVLRQRVSIDIPALSP
AAHVVLERVRDAIDRNDLPAAMAYALSNRETKLEIDGFNQAVTERFGERTLLTNAAREPSGKLFDKLSEG
MQPEQKERLKEAWPIMRTSQQLAAHERTVQTLRQAEDLRLAQRQTPVMKQ
Protein domains
Predicted by InterproScan.
Protein structure
No available structure.
T4CP
| ID | 14060 | GenBank | WP_065657821 |
| Name | traG_A6U93_RS27320_pAt15-1187-1-2a2 |
UniProt ID | _ |
| Length | 657 a.a. | PDB ID | _ |
| Note | Predicted by oriTfinder 2.0 | ||
T4CP protein sequence
Download Length: 657 a.a. Molecular weight: 70493.46 Da Isoelectric Point: 9.1158
>WP_065657821.1 Ti-type conjugative transfer system protein TraG [Agrobacterium tumefaciens]
MTASKVFLAVIPATTMIVVVVFMPGIEHWLAAVGKTAQAKLMLGRVGLALTYATAAVIGTIFLFAANGAA
GIKAAGWGVVGGDGVAILIAVLREGVRLAGIAGDIPKGQSVLGYADPATMLGASTAFLAGVFALRVAVMG
NAAFAKAAPRRIGGKRAVHGEADWMKVQEAAKLFPDPGGIVVGERYRVDRDSVAAVSFRADDPSTWGAGG
KSPLLCFDGSFGSSHGIVFAGSGGFKTTSVTVPTALKWGGGLVVLDPSSEVAPMVSEHRRKAGRTVIILD
PTASSVGFNALDWIGRHGSTKEEDIVAVATWIMTDNPRTASARDDFFRASAMQLLTALIADVCLSGHTDE
EDQTLRRVRTNLSEPEPQLRARLTKIYEQSESDFVKENVSVFVNMTPETFSGVYANAVKETHWLSYPNYA
ALVSGDSFSTDDLADGGTDIFIALDLKVLEAHPGLARVVIGSLLNAIYNRNGDVKGRALFLLDEVARLGY
LRILETARDAGRKYGITLTMIFQSIGQMREAYGGRDATSKWFESASWISFAAINDPDTADYISKRCGDTT
VEVDQTNRSSGMKGSSRSRSRQLSRRPLILPHEVLRMRADEQIVFTSGNPPLRCGRAIWFRRKDMSASVG
ENRFHKQATEGVKSYKAAPKDNEETKT
MTASKVFLAVIPATTMIVVVVFMPGIEHWLAAVGKTAQAKLMLGRVGLALTYATAAVIGTIFLFAANGAA
GIKAAGWGVVGGDGVAILIAVLREGVRLAGIAGDIPKGQSVLGYADPATMLGASTAFLAGVFALRVAVMG
NAAFAKAAPRRIGGKRAVHGEADWMKVQEAAKLFPDPGGIVVGERYRVDRDSVAAVSFRADDPSTWGAGG
KSPLLCFDGSFGSSHGIVFAGSGGFKTTSVTVPTALKWGGGLVVLDPSSEVAPMVSEHRRKAGRTVIILD
PTASSVGFNALDWIGRHGSTKEEDIVAVATWIMTDNPRTASARDDFFRASAMQLLTALIADVCLSGHTDE
EDQTLRRVRTNLSEPEPQLRARLTKIYEQSESDFVKENVSVFVNMTPETFSGVYANAVKETHWLSYPNYA
ALVSGDSFSTDDLADGGTDIFIALDLKVLEAHPGLARVVIGSLLNAIYNRNGDVKGRALFLLDEVARLGY
LRILETARDAGRKYGITLTMIFQSIGQMREAYGGRDATSKWFESASWISFAAINDPDTADYISKRCGDTT
VEVDQTNRSSGMKGSSRSRSRQLSRRPLILPHEVLRMRADEQIVFTSGNPPLRCGRAIWFRRKDMSASVG
ENRFHKQATEGVKSYKAAPKDNEETKT
Protein domains
Predicted by InterproScan.
Protein structure
No available structure.
| ID | 14061 | GenBank | WP_065657871 |
| Name | t4cp2_A6U93_RS27600_pAt15-1187-1-2a2 |
UniProt ID | _ |
| Length | 822 a.a. | PDB ID | _ |
| Note | Predicted by oriTfinder 2.0 | ||
T4CP protein sequence
Download Length: 822 a.a. Molecular weight: 91939.91 Da Isoelectric Point: 5.8678
>WP_065657871.1 conjugal transfer protein TrbE [Agrobacterium tumefaciens]
MVALKSFRHSGPSFADLVPYAGLVDNGVILLKDGSLMAGWYFAGPDSESSTDAERNEVSRQINAILSRLG
SGWMIQVEAIRVPTGNYPDEAESHFPDPVTRAIDDERRVHFQKERGHFESRHALILTWRPPEPRRSGLTR
YVYSDAASRSATYADKALESFLTSIREVEQYLANVVSIHRMMTRETPERGGFRVARYDELFQFIRFCVTG
ENHPVRLPDIPMYLDWLVTAELQHGLTPMVENRFLGVVAIDGLPAESWPGILNTLDRMPLTYRWSSRFVF
LDTEEARARLERTRKKWQQKVRPFFDQLFQTQSRSVDQDAMLMVAETEDAIAEASSQLVAYGYYTPVIVI
FEEDPTRLQEKCEAIRRLIQAEGFGARIETLNATDAFLGSLPGVSYANIREPLINTRNLADLIPLNSVWS
GSPVAPCPFYPPASPPLMQVASGSTPFRLNLHVDDVGHTLIFGPTGSGKSTLLALIAAQFRRYADAQVFA
FDKGGSMLPLTLGLDGDHYQIGGDIGASAIGEGKVLAFCPLAELSTDGDRAWASEWIEMLVALQGVTITP
DYRNAISRQIALMAESRGRSLSDFVSGVQMREIKDALHHYTVDGPMGQLLDAEQDGLVLGAFQCFEVEEL
MNMGERNLVPVLTYLFRRIEKRLTGAPSLIILDEAWLMLGHPVFRDKIREWLKVLRKANCAVVLATQSIS
DAERSGIIDVLKESCPTKICLPNGAAREPGTREFYERIGFNERQIEIVATAIPKREYYVVSPEGRRLFNM
ALGPIALSFVGATGRQDLKHIRSLHSEYGAAWPLHWLQQRGIAHAETLFPTS
MVALKSFRHSGPSFADLVPYAGLVDNGVILLKDGSLMAGWYFAGPDSESSTDAERNEVSRQINAILSRLG
SGWMIQVEAIRVPTGNYPDEAESHFPDPVTRAIDDERRVHFQKERGHFESRHALILTWRPPEPRRSGLTR
YVYSDAASRSATYADKALESFLTSIREVEQYLANVVSIHRMMTRETPERGGFRVARYDELFQFIRFCVTG
ENHPVRLPDIPMYLDWLVTAELQHGLTPMVENRFLGVVAIDGLPAESWPGILNTLDRMPLTYRWSSRFVF
LDTEEARARLERTRKKWQQKVRPFFDQLFQTQSRSVDQDAMLMVAETEDAIAEASSQLVAYGYYTPVIVI
FEEDPTRLQEKCEAIRRLIQAEGFGARIETLNATDAFLGSLPGVSYANIREPLINTRNLADLIPLNSVWS
GSPVAPCPFYPPASPPLMQVASGSTPFRLNLHVDDVGHTLIFGPTGSGKSTLLALIAAQFRRYADAQVFA
FDKGGSMLPLTLGLDGDHYQIGGDIGASAIGEGKVLAFCPLAELSTDGDRAWASEWIEMLVALQGVTITP
DYRNAISRQIALMAESRGRSLSDFVSGVQMREIKDALHHYTVDGPMGQLLDAEQDGLVLGAFQCFEVEEL
MNMGERNLVPVLTYLFRRIEKRLTGAPSLIILDEAWLMLGHPVFRDKIREWLKVLRKANCAVVLATQSIS
DAERSGIIDVLKESCPTKICLPNGAAREPGTREFYERIGFNERQIEIVATAIPKREYYVVSPEGRRLFNM
ALGPIALSFVGATGRQDLKHIRSLHSEYGAAWPLHWLQQRGIAHAETLFPTS
Protein domains
Predicted by InterproScan.
Protein structure
No available structure.
T4SS
T4SS were predicted by using oriTfinder2.
Region 1: 116154..125846
| Locus tag | Coordinates | Strand | Size (bp) | Protein ID | Product | Description |
|---|---|---|---|---|---|---|
| A6U93_RS27540 (A6U93_27540) | 111369..112460 | - | 1092 | WP_065657860 | Gfo/Idh/MocA family protein | - |
| A6U93_RS27545 (A6U93_27545) | 112573..113352 | - | 780 | WP_065657861 | ATP-binding cassette domain-containing protein | - |
| A6U93_RS27550 (A6U93_27550) | 113352..114464 | - | 1113 | WP_065657862 | ABC transporter permease | - |
| A6U93_RS27555 (A6U93_27555) | 114536..115489 | - | 954 | WP_065657863 | sugar ABC transporter substrate-binding protein | - |
| A6U93_RS27560 (A6U93_27560) | 115714..116085 | + | 372 | WP_065657864 | hypothetical protein | - |
| A6U93_RS27565 (A6U93_27565) | 116154..117476 | - | 1323 | WP_065657865 | IncP-type conjugal transfer protein TrbI | virB10 |
| A6U93_RS27570 (A6U93_27570) | 117488..117964 | - | 477 | WP_065657866 | conjugal transfer protein TrbH | - |
| A6U93_RS27575 (A6U93_27575) | 117964..118815 | - | 852 | WP_065657884 | P-type conjugative transfer protein TrbG | virB9 |
| A6U93_RS27580 (A6U93_27580) | 118833..119510 | - | 678 | WP_065657867 | conjugal transfer protein TrbF | virB8 |
| A6U93_RS27585 (A6U93_27585) | 119528..120718 | - | 1191 | WP_065657868 | P-type conjugative transfer protein TrbL | virB6 |
| A6U93_RS27590 (A6U93_27590) | 120712..120939 | - | 228 | WP_065657869 | entry exclusion protein TrbK | - |
| A6U93_RS27595 (A6U93_27595) | 120936..121745 | - | 810 | WP_065657870 | P-type conjugative transfer protein TrbJ | virB5 |
| A6U93_RS27600 (A6U93_27600) | 121717..124185 | - | 2469 | WP_065657871 | conjugal transfer protein TrbE | virb4 |
| A6U93_RS27605 (A6U93_27605) | 124189..124488 | - | 300 | WP_065657872 | conjugal transfer protein TrbD | virB3 |
| A6U93_RS27610 (A6U93_27610) | 124481..124885 | - | 405 | WP_065657873 | conjugal transfer pilin TrbC | virB2 |
| A6U93_RS27615 (A6U93_27615) | 124875..125846 | - | 972 | WP_065658927 | P-type conjugative transfer ATPase TrbB | virB11 |
| A6U93_RS27620 (A6U93_27620) | 125843..126478 | - | 636 | WP_065657771 | acyl-homoserine-lactone synthase | - |
Host bacterium
| ID | 19380 | GenBank | NZ_CP123841 |
| Plasmid name | pAt15-1187-1-2a2 | Incompatibility group | - |
| Plasmid size | 126855 bp | Coordinate of oriT [Strand] | 67755..67798 [+] |
| Host baterium | Agrobacterium tumefaciens strain 15-1187-1-2a |
Cargo genes
| Drug resistance gene | - |
| Virulence gene | - |
| Metal resistance gene | arsC |
| Degradation gene | - |
| Symbiosis gene | - |
| Anti-CRISPR | - |