Detailed information of oriT

oriT

The information of the oriT region


oriTDB ID   118918
Name   oriT_punidentified in_silico
Organism   Enterobacter cloacae strain 2312040
Sequence Completeness      -
NCBI accession of oriT (coordinates [strand])   NZ_CP109675 (62163..62249 [+], 87 nt)
oriT length   87 nt
IRs (inverted repeats)     _
Location of nic site      _
Conserved sequence flanking the
  nic site    		  _
Note   Predicted by oriTfinder 2.0

  oriT sequence  


Download         Length: 87 nt

>oriT_punidentified
GGGTGTCGGGGCGGAGCCCTGACCAGGTGGCATTTGTGATATCGTGCGTGCGCGGTATTACAAATGCACATCCTGTCCCGTCTTTCG

Visualization of oriT structure

  oriT secondary structure

Predicted by RNAfold.

Download structure file


Relaxase

ID   11988 GenBank   WP_022650008
Name   Relaxase_OKD03_RS00295_punidentified insolico UniProt ID   A0A4Q2QTK9
Length   899 a.a. PDB ID   
Note   Predicted by oriTfinder 2.0

  Relaxase protein sequence


Download         Length: 899 a.a.        Molecular weight: 102314.37 Da        Isoelectric Point: 8.2756

>WP_022650008.1 MULTISPECIES: relaxase/mobilization nuclease domain-containing protein [Enterobacterales]
MIPVIPPKRQDGKSSFGDLVSYVSVRDEPRDDDLMEAVRASGTKPEMPHSSRFSRLVDYATKLRDESFIS
LVDVMPDGGEWVNFYGVTCFHNCTSIESAAEEMEFSARKTKFAHNKSDPVFHYILSWQSHESPRPEQIYD
SVSHTLSRLGLAGHQFVAAVHTDTDNLHVHIAVNRVHPETGYLNRLAYSQEKLSKACRELELKHGFSPDN
GCYVIAPDNRIVRRTSVERDRQSAWKRGRNQSLREYIADTAIAGLRESPVTDWSSLHQRFAKEGLFLTKE
SGELKVKDGWDSERPGVTLSAFGHAFDTDKLIRKLGEFVPPVQDIFSQVPAVGRYEPDKIIMPSRPERMT
EKESLSDYAIARLRAPLIALDQDPTQRTIQSVHTLLAQSGLYLKEQHDRLVICDGYDSTRTPVRAERVWP
ALTKAILDSYKGGWQPVPKDIFQQVPPAEQFTGGSLEATPVSDREWRKLRTGSGPQGALKREIFSDKESL
WGYAVSHCRQDIESLIGSGEFTWERCHELLARQGLLLMREQQGLVVMDAYNHEQTPVKASHVHPDLTLAR
AEPHAGSFVSVPADIFERVKPASRYNPELAVSDRDIPGMKRDPELRRQRREARAAAREDLKARYAAWRAN
WQRPDLHGRERYQAIHDECRIRKARIRIEHRDPLVRKLHYHIAELQRMQALIALKETMKAERAELIGQGK
WYPPSYRQWVEAEAVKGDKAAISQLRGWDYRDRRGNAVKSTTDNRCVVICEPGGTPMYSGVPGLRASLKK
NGRVQFRDPETGRHICTDYGDRVIFRNSGDYDALKRDMVKVAPVLFSRSPSVAMAPEGNNEQFNTAFAKM
VAWHNVNHRDEGEYQISRPEVDQVRVESEMRFAEMTNQGSNTEAVRNESGPNWKPPSPL

  Protein domains


Predicted by InterproScan.

Relaxase/Mobilisation nuclease domain (Relaxase)

(51-317)


  Protein structure


Source ID Structure
AlphaFold DB A0A4Q2QTK9


T4CP

ID   14038 GenBank   WP_022650006
Name   trbC_OKD03_RS00285_punidentified insolico UniProt ID   _
Length   766 a.a. PDB ID   _
Note   Predicted by oriTfinder 2.0

  T4CP protein sequence


Download         Length: 766 a.a.        Molecular weight: 87660.94 Da        Isoelectric Point: 6.8660

>WP_022650006.1 MULTISPECIES: F-type conjugative transfer protein TrbC [Enterobacterales]
MNYTPVNQALMKRNAWNSPVLELLRDHLLYAGIMAGSVVAGFIWPLAIPLLLLLAIVASISFGTHRWRMP
MRMPVHLNKVDPSEDRKVRRSLFRRFPSLFQYETTQESKGKGIFYLGYRRMNDIGRELWLTIDDLTRHVM
FFASTGGGKTETIYAWMLNSFCWGRGFTFVDGKAQNDTARTCYYLARRFGREDDVEYINFMNSGMSRSEM
IHNGDKSRPQSHQWNPFCYSTEAFVAETMQSMLPTNVQGGEWQSRAIAMNKALVFGTKFYCVRENKTMSL
QLLREFMPLEKLAELYCRAIDDQWPEEAVSPLYNYLVDVPGFDMATVRQPSAWTEEPRKQHSYLTGQFLE
TFNTFTETFGNIFAEDAGDIDIRDSIHSDRILLVLIPAMNTSQHTTSALGRMFVTQQSMILARDLGHRLE
GLDSEALEITKYKGPFPYMNYLDEVGAYYTERIAVQATQVRSLDFSIVMMSQDQERIESQTSATNVATLM
QNAGTKVAGKIVSDDKTAKTITNAAGQEARANMVSLQRRDGIIGTSWIDGDHINIQMENKINVQDLIKLQ
PGENYTVFQGDPVPGASFYIEPQDKTCNAPVIINRYITINPPNLKQLRKLVPRTAQRRLPAPENVSKIIG
VLTEKPSRRRKAAKAEPWKIIDTFQQRLANRQEAHNLMTEYDTDHRGREENLWEEALEVIRTTTMAERTI
RYITLNKPESEPTQVSAEEIQLAPDAILRTLTLPQPAYIPPSRYRNEPFISPKLPDSPHPDMEWPE

  Protein domains


Predicted by InterproScan.

TraM recognition site of TraD and TraG (TraG-D_C)

(440-528)

  Protein structure



No available structure.




T4SS

T4SS were predicted by using oriTfinder2.

Region 1: 21596..56040

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
OKD03_RS00105 (OKD03_00095) 17660..18097 + 438 WP_022649971 type IV pilus biogenesis protein PilM -
OKD03_RS00110 (OKD03_00100) 18112..19788 + 1677 WP_022649972 PilN family type IVB pilus formation outer membrane protein -
OKD03_RS00115 (OKD03_00105) 19790..21094 + 1305 WP_022649973 type 4b pilus protein PilO2 -
OKD03_RS00120 (OKD03_00110) 21078..21593 + 516 WP_022649974 type IV pilus biogenesis protein PilP -
OKD03_RS00125 (OKD03_00115) 21596..23155 + 1560 WP_022649975 GspE/PulE family protein virB11
OKD03_RS00130 (OKD03_00120) 23148..24242 + 1095 WP_032623575 type II secretion system F family protein -
OKD03_RS00135 (OKD03_00125) 24256..24870 + 615 WP_022649977 type 4 pilus major pilin -
OKD03_RS00140 (OKD03_00130) 24955..25437 + 483 WP_045261878 lytic transglycosylase domain-containing protein virB1
OKD03_RS00145 (OKD03_00135) 25422..26096 + 675 WP_022649979 prepilin peptidase -
OKD03_RS00150 (OKD03_00140) 26093..27430 + 1338 WP_022649980 shufflon system plasmid conjugative transfer pilus tip adhesin PilV -
OKD03_RS00155 (OKD03_00145) 28008..29168 + 1161 WP_265937802 site-specific integrase -
OKD03_RS00160 (OKD03_00150) 29245..29664 + 420 WP_071785236 hypothetical protein -
OKD03_RS00165 (OKD03_00155) 29811..30647 + 837 WP_022649983 hypothetical protein traE
OKD03_RS00170 (OKD03_00160) 30753..31958 + 1206 WP_022649984 conjugal transfer protein TraF -
OKD03_RS00175 (OKD03_00165) 32067..32483 + 417 WP_123839394 hypothetical protein -
OKD03_RS00180 (OKD03_00170) 32554..33027 + 474 WP_022649985 DotD/TraH family lipoprotein -
OKD03_RS00185 (OKD03_00175) 33024..33830 + 807 WP_022649986 type IV secretory system conjugative DNA transfer family protein traI
OKD03_RS00190 (OKD03_00180) 33843..35003 + 1161 WP_022649987 plasmid transfer ATPase TraJ virB11
OKD03_RS00195 (OKD03_00185) 35000..35287 + 288 WP_022649988 hypothetical protein traK
OKD03_RS00200 (OKD03_00190) 35359..38901 + 3543 WP_032635082 LPD7 domain-containing protein -
OKD03_RS00205 (OKD03_00195) 38931..39269 + 339 WP_086626045 conjugal transfer protein traL
OKD03_RS00210 (OKD03_00200) 39275..39976 + 702 WP_022649991 DotI/IcmL family type IV secretion protein traM
OKD03_RS00215 (OKD03_00205) 39987..40967 + 981 WP_022649992 DotH/IcmK family type IV secretion protein traN
OKD03_RS00220 (OKD03_00210) 40967..42274 + 1308 WP_022649993 conjugal transfer protein TraO traO
OKD03_RS00225 (OKD03_00215) 42255..43028 + 774 WP_123839398 conjugal transfer protein TraP traP
OKD03_RS00230 (OKD03_00220) 43028..43552 + 525 WP_022649995 conjugal transfer protein TraQ traQ
OKD03_RS00235 (OKD03_00225) 43587..43991 + 405 WP_022649996 DUF6750 family protein traR
OKD03_RS00240 (OKD03_00230) 44175..44900 + 726 WP_063864836 hypothetical protein traT
OKD03_RS00245 (OKD03_00235) 44987..48040 + 3054 WP_022649998 hypothetical protein traU
OKD03_RS00250 (OKD03_00240) 48040..48609 + 570 WP_022649999 hypothetical protein traV
OKD03_RS00255 (OKD03_00245) 48622..49824 + 1203 WP_022650000 conjugal transfer protein TraW traW
OKD03_RS00260 (OKD03_00250) 49821..50363 + 543 WP_022650001 conjugal transfer protein TraX -
OKD03_RS00265 (OKD03_00255) 50421..52580 + 2160 WP_022650002 DotA/TraY family protein traY
OKD03_RS00270 (OKD03_00260) 52642..53274 + 633 WP_022650003 plasmid IncI1-type surface exclusion protein ExcA -
OKD03_RS00275 (OKD03_00265) 53595..54803 + 1209 WP_022650004 hypothetical protein trbA
OKD03_RS00280 (OKD03_00270) 54784..56040 + 1257 WP_126530067 hypothetical protein trbB
OKD03_RS00285 (OKD03_00275) 56044..58344 + 2301 WP_022650006 F-type conjugative transfer protein TrbC -
OKD03_RS00290 (OKD03_00280) 58418..58687 + 270 WP_022650007 hypothetical protein -

Host bacterium

ID   19350 GenBank   NZ_CP109675
Plasmid name   punidentified Incompatibility group   -
Plasmid size   106687 bp Coordinate of oriT [Strand]   62163..62249 [+]
Host baterium   Enterobacter cloacae strain 2312040

Cargo genes

Drug resistance gene   qnrS1, blaLAP-2
Virulence gene   -
Metal resistance gene   -
Degradation gene   -
Symbiosis gene   -
Anti-CRISPR   AcrIIA7, AcrIIC1