Detailed information of oriT
oriT
The information of the oriT region
| oriTDB ID | 118871 |
| Name | oriT_pHNNC189-4  |
| Organism | Raoultella ornithinolytica strain NC189 |
| Sequence Completeness | - |
| NCBI accession of oriT (coordinates [strand]) | NZ_MN548433 (61102..61151 [-], 50 nt) |
| oriT length | 50 nt |
| IRs (inverted repeats) | 7..14, 17..24 (GCAAAATT..AATTTTGC) |
| Location of nic site | 33..34 |
| Conserved sequence flanking the nic site |
TGTGTGGTGA |
| Note | Predicted by oriTfinder 2.0 |
oriT sequence
Download Length: 50 nt
>oriT_pHNNC189-4
AAATCTGCAAAATTTTAATTTTGCGTAGTGTGTGGTGATTTTGTGGTGAG
AAATCTGCAAAATTTTAATTTTGCGTAGTGTGTGGTGATTTTGTGGTGAG
Visualization of oriT structure
oriT secondary structure
Predicted by RNAfold.
Download structure file
Relaxase
| ID | 11964 | GenBank | WP_075606914 |
| Name | traI_KM111_RS00530_pHNNC189-4 |
UniProt ID | A0A6M3HIK1 |
| Length | 1752 a.a. | PDB ID | |
| Note | Predicted by oriTfinder 2.0 | ||
Relaxase protein sequence
Download Length: 1752 a.a. Molecular weight: 190386.65 Da Isoelectric Point: 5.5084
>WP_075606914.1 MULTISPECIES: conjugative transfer relaxase/helicase TraI [Enterobacteriaceae]
MLSFSQVKSAGSAGNYYTEKDNYYVIGSMEERWQGKGAEALGLEGKVDKQVFTELLQGKLPDGSDLTRMQ
DGVNKHRPGYDLTFSAPKSVSMLAMLGGDKRLIDAHNRAVTVALNQVESLASTRVQKDGVSETVLTNNLI
IARFNHDTSRAQEPQIHTHSVVINATQNGDKWQTLGSDTVGKTGFSENILANRIAFGKIYQSTLRADVES
MGYKTVDAGKHGMWEMEGVPVESFSTRSQELREAAGPDASLKSRDVAALDTRKSKEAIDPAEKMVEWMNT
LKETGFDIRGYREAADTRAKELASAPAAPVKTDVPDISDVVTKAIAGLSDRKVQFTYADLLARTVSQLEA
KPGVFEQARTGIDAAIEREQLIPLDREKGLFTSNIHVLDELSVKALSQEIQRQNHVSVTPDTAVVRQSPF
SDAVSVLAQDRPTMGIVSGQGGAAGQRERVAELTLMAREQGRDVHIIAADNRSREFLSADARLAGETVTG
KSALQDGTAFIPGGTLIVDQAEKMSLKETLSMLDGAMRHNVQVLLSDSGKRSGTGSALTVLKESGVNTYR
WQGGKQATADIISEPEKGARYSRLAQEFAASVREGQESVAQISGSREQGILNGLIRETLKSEGVLGEKDM
TVTALTPVWLDSKSRGVRDYYREGMVMERWDPETRQHDRFVIDRVTASSNMLTLRDKEGERLDMKVSAVD
SQWTLFRADKLTVAEGERLAVLGKIPDTRLKGGESVTVLKAEEGQLTVQRPGQKTTQSLSVGSSPFDGIK
VGHGWVESPGRSVSETATVFASVTQRELDNATLNQLAQSGSHIRLYSAQGTARTTEKLARHTAFSVVSEQ
LKARSGETDLDAAIASQKAGLHTPAEQVIHLSIPLLESEELTFTRPQLLATALETGGGKVPMTEIDATIQ
SQIQAGSLLNVPVAPGHGNDLLISRQTWDAEKSILTKVLEGKEAVAPLMDNVPGSLMTDLTSGQRASTRM
ILETTDRFTVVQGYAGVGKTTQFRAVTGAISLLPEATRPRVIGLGPTHRAVGEMQSAGVDAQTTASFLHD
TQLLQRNGQTPDFSNTLFLLDESSMVGLADTAKALSLIAAGGGRAVLSGDTDQLQSIAPGQPFRLMQQRS
AADVAIMKEIVRQVPELRPAVYSLIDRDVNRALATIEQVTPEQVPRKDGAWVPENSVVEFTPAQEEAIQK
ALNKGESVPAGQPTTLYEALVKDYADRTPEAQSQTLIITHLNDDRRVLNSLIHDARRDNGEVGREEVTLP
VLVTSNIRDGELRRLSTWTAHKGAVALVDNVYHRIGSVDKENQLITLMDGEGKERLISPREALAEGVTLY
REQEITVSQGDKMRFSKSDTERGYVANSVWEVKSVSGDSVTLSDGKSTRTLNPKADQAQQHIDLAYAITA
HGAQGASEPFAIALEGVAGGRERMASFESAYVGLSRMKQHVQVYTDNREGWIKAINNSPEKATAHDILEP
RNDRAVKTAGQLFSRARALDEAAAGRAALQQSGLARGQSPGKFISPGKKYPQPHVALPAFDKNGKDAGIW
LSPLTDNDGRLQVIGGEGRIMGNEDARFVALQNSRNGESLLAGNMGEGVRIARDNPDSGVVVRLAGDDRP
WNPEAITGGRIWADPLPNPPINDTGTDIPLPPEVLAQRAAEEAQRREMERQTEQATREVSGEEKKAGEPG
ERIKEVIGDVIRGLERDRPGEEKTVLPDDPQTRRQEAAVQQVVSESLQRDRLQQMERDMVRDLNREKTLG
GD
MLSFSQVKSAGSAGNYYTEKDNYYVIGSMEERWQGKGAEALGLEGKVDKQVFTELLQGKLPDGSDLTRMQ
DGVNKHRPGYDLTFSAPKSVSMLAMLGGDKRLIDAHNRAVTVALNQVESLASTRVQKDGVSETVLTNNLI
IARFNHDTSRAQEPQIHTHSVVINATQNGDKWQTLGSDTVGKTGFSENILANRIAFGKIYQSTLRADVES
MGYKTVDAGKHGMWEMEGVPVESFSTRSQELREAAGPDASLKSRDVAALDTRKSKEAIDPAEKMVEWMNT
LKETGFDIRGYREAADTRAKELASAPAAPVKTDVPDISDVVTKAIAGLSDRKVQFTYADLLARTVSQLEA
KPGVFEQARTGIDAAIEREQLIPLDREKGLFTSNIHVLDELSVKALSQEIQRQNHVSVTPDTAVVRQSPF
SDAVSVLAQDRPTMGIVSGQGGAAGQRERVAELTLMAREQGRDVHIIAADNRSREFLSADARLAGETVTG
KSALQDGTAFIPGGTLIVDQAEKMSLKETLSMLDGAMRHNVQVLLSDSGKRSGTGSALTVLKESGVNTYR
WQGGKQATADIISEPEKGARYSRLAQEFAASVREGQESVAQISGSREQGILNGLIRETLKSEGVLGEKDM
TVTALTPVWLDSKSRGVRDYYREGMVMERWDPETRQHDRFVIDRVTASSNMLTLRDKEGERLDMKVSAVD
SQWTLFRADKLTVAEGERLAVLGKIPDTRLKGGESVTVLKAEEGQLTVQRPGQKTTQSLSVGSSPFDGIK
VGHGWVESPGRSVSETATVFASVTQRELDNATLNQLAQSGSHIRLYSAQGTARTTEKLARHTAFSVVSEQ
LKARSGETDLDAAIASQKAGLHTPAEQVIHLSIPLLESEELTFTRPQLLATALETGGGKVPMTEIDATIQ
SQIQAGSLLNVPVAPGHGNDLLISRQTWDAEKSILTKVLEGKEAVAPLMDNVPGSLMTDLTSGQRASTRM
ILETTDRFTVVQGYAGVGKTTQFRAVTGAISLLPEATRPRVIGLGPTHRAVGEMQSAGVDAQTTASFLHD
TQLLQRNGQTPDFSNTLFLLDESSMVGLADTAKALSLIAAGGGRAVLSGDTDQLQSIAPGQPFRLMQQRS
AADVAIMKEIVRQVPELRPAVYSLIDRDVNRALATIEQVTPEQVPRKDGAWVPENSVVEFTPAQEEAIQK
ALNKGESVPAGQPTTLYEALVKDYADRTPEAQSQTLIITHLNDDRRVLNSLIHDARRDNGEVGREEVTLP
VLVTSNIRDGELRRLSTWTAHKGAVALVDNVYHRIGSVDKENQLITLMDGEGKERLISPREALAEGVTLY
REQEITVSQGDKMRFSKSDTERGYVANSVWEVKSVSGDSVTLSDGKSTRTLNPKADQAQQHIDLAYAITA
HGAQGASEPFAIALEGVAGGRERMASFESAYVGLSRMKQHVQVYTDNREGWIKAINNSPEKATAHDILEP
RNDRAVKTAGQLFSRARALDEAAAGRAALQQSGLARGQSPGKFISPGKKYPQPHVALPAFDKNGKDAGIW
LSPLTDNDGRLQVIGGEGRIMGNEDARFVALQNSRNGESLLAGNMGEGVRIARDNPDSGVVVRLAGDDRP
WNPEAITGGRIWADPLPNPPINDTGTDIPLPPEVLAQRAAEEAQRREMERQTEQATREVSGEEKKAGEPG
ERIKEVIGDVIRGLERDRPGEEKTVLPDDPQTRRQEAAVQQVVSESLQRDRLQQMERDMVRDLNREKTLG
GD
Protein domains
Predicted by InterproScan.
Protein structure
No available structure.
Auxiliary protein
| ID | 6845 | GenBank | WP_053390193 |
| Name | WP_053390193_pHNNC189-4 |
UniProt ID | A0A8G2A327 |
| Length | 130 a.a. | PDB ID | _ |
| Note | Predicted by oriTfinder 2.0 | ||
Auxiliary protein sequence
Download Length: 130 a.a. Molecular weight: 14883.89 Da Isoelectric Point: 4.3779
>WP_053390193.1 MULTISPECIES: conjugal transfer relaxosome DNA-binding protein TraM [Enterobacteriaceae]
MAKVQAYVSDEVADKINAIVEKRRVEGAKDKDISFSSISTMLLELGLRVYEAQMERKESGFNQMAFNKAL
LESVIKTQFTVNKVLGIECLSPHVSGNPRWEWPGLIENIRDDVQEVMLRFFPDEESEDEE
MAKVQAYVSDEVADKINAIVEKRRVEGAKDKDISFSSISTMLLELGLRVYEAQMERKESGFNQMAFNKAL
LESVIKTQFTVNKVLGIECLSPHVSGNPRWEWPGLIENIRDDVQEVMLRFFPDEESEDEE
Protein domains
Predicted by InterproScan.
Protein structure
No available structure.
T4CP
| ID | 14011 | GenBank | WP_075606915 |
| Name | traD_KM111_RS00525_pHNNC189-4 |
UniProt ID | _ |
| Length | 772 a.a. | PDB ID | _ |
| Note | Predicted by oriTfinder 2.0 | ||
T4CP protein sequence
Download Length: 772 a.a. Molecular weight: 86712.47 Da Isoelectric Point: 5.0825
>WP_075606915.1 MULTISPECIES: type IV conjugative transfer system coupling protein TraD [Enterobacteriaceae]
MSFNAKDMTQGGQIANMRFRMFGQIANIIFYVLFIIFWVLCGLILMYRLSWQTFVNGCVYWWCTTLAPMR
DIIRSQPVYTINYYGKSLEYNSEQILADKYTIWCGEQLWTSFVFAAIVSLTVCIVTFFVASWVLGRQGKQ
QSEDENTGGRQLSDKPKEVARQMKRDGVASDIKIGDLPILKHSEIQNFCLHGTVGSGKSEVIRRLLNYVR
ARGDMAIIYDRSCEFVKSYYDPSLDKILNPLDSRCAAWDIWRECLTLPDFDNVSNTLIPMGTKEDPFWQG
SGRTIFSEGAYLMREDKDRSYEKLVDTMLSIKIDKLRAYLKNTPAANLVEEKIEKTAISIRAVLTNYVKA
IRYLQGIEKNGEPFTIRDWMRGVREDQPNGWLFISSNADTHASLKPVISMWLSIAIRGLLGMGENRNRRV
WIFADELPTLHKLPDLVEILPEARKFGGCYVFGIQSYAQLEDIYGVKPAATLFDVMNTRAFFRSPSKEIA
EFAAGEIGEKEILKASEQYSYGADPVRDGVSTGKEKERETLVSYSDIQTLPDLSCYVTLPGPYPAVKLAL
KYKSRPKIAEGFILRNLDTRTDARLDTLLEAREAESSHARTLFTPDAPAPELAEKDEKVGNPPEQSQPAE
SSGTPATVKVPSTAKTPEADESGQSPEVSNPAALLTKVVTVPLTRPRPAAATGTAAVASATDVRATPAGG
TEQALETQPAEQGQDMLPPGVNEYGEIDDMHAWDEWQSGEQTQRDMQRREEVNINHSHRRDEQDDIEIGG
NF
MSFNAKDMTQGGQIANMRFRMFGQIANIIFYVLFIIFWVLCGLILMYRLSWQTFVNGCVYWWCTTLAPMR
DIIRSQPVYTINYYGKSLEYNSEQILADKYTIWCGEQLWTSFVFAAIVSLTVCIVTFFVASWVLGRQGKQ
QSEDENTGGRQLSDKPKEVARQMKRDGVASDIKIGDLPILKHSEIQNFCLHGTVGSGKSEVIRRLLNYVR
ARGDMAIIYDRSCEFVKSYYDPSLDKILNPLDSRCAAWDIWRECLTLPDFDNVSNTLIPMGTKEDPFWQG
SGRTIFSEGAYLMREDKDRSYEKLVDTMLSIKIDKLRAYLKNTPAANLVEEKIEKTAISIRAVLTNYVKA
IRYLQGIEKNGEPFTIRDWMRGVREDQPNGWLFISSNADTHASLKPVISMWLSIAIRGLLGMGENRNRRV
WIFADELPTLHKLPDLVEILPEARKFGGCYVFGIQSYAQLEDIYGVKPAATLFDVMNTRAFFRSPSKEIA
EFAAGEIGEKEILKASEQYSYGADPVRDGVSTGKEKERETLVSYSDIQTLPDLSCYVTLPGPYPAVKLAL
KYKSRPKIAEGFILRNLDTRTDARLDTLLEAREAESSHARTLFTPDAPAPELAEKDEKVGNPPEQSQPAE
SSGTPATVKVPSTAKTPEADESGQSPEVSNPAALLTKVVTVPLTRPRPAAATGTAAVASATDVRATPAGG
TEQALETQPAEQGQDMLPPGVNEYGEIDDMHAWDEWQSGEQTQRDMQRREEVNINHSHRRDEQDDIEIGG
NF
Protein domains
Predicted by InterproScan.
Protein structure
No available structure.
T4SS
T4SS were predicted by using oriTfinder2.
Region 1: 60544..88288
| Locus tag | Coordinates | Strand | Size (bp) | Protein ID | Product | Description |
|---|---|---|---|---|---|---|
| KM111_RS00325 | 55646..55996 | + | 351 | WP_004153414 | hypothetical protein | - |
| KM111_RS00330 | 56626..56979 | + | 354 | WP_004152748 | hypothetical protein | - |
| KM111_RS00335 | 57036..57383 | + | 348 | WP_004152749 | hypothetical protein | - |
| KM111_RS00340 | 57478..57624 | + | 147 | WP_004152750 | hypothetical protein | - |
| KM111_RS00345 | 57675..58508 | + | 834 | WP_004152751 | N-6 DNA methylase | - |
| KM111_RS00590 | 58590..58715 | - | 126 | WP_257792838 | hypothetical protein | - |
| KM111_RS00350 | 59328..60149 | + | 822 | WP_004152492 | DUF932 domain-containing protein | - |
| KM111_RS00355 | 60182..60511 | + | 330 | WP_011977736 | DUF5983 family protein | - |
| KM111_RS00360 | 60544..61029 | - | 486 | WP_001568108 | transglycosylase SLT domain-containing protein | virB1 |
| KM111_RS00365 | 61463..61855 | + | 393 | WP_053390193 | conjugal transfer relaxosome DNA-binding protein TraM | - |
| KM111_RS00370 | 62094..62768 | + | 675 | WP_075606928 | hypothetical protein | - |
| KM111_RS00375 | 62962..63123 | + | 162 | WP_172686697 | TraY domain-containing protein | - |
| KM111_RS00380 | 63188..63556 | + | 369 | WP_053390195 | type IV conjugative transfer system pilin TraA | - |
| KM111_RS00385 | 63570..63875 | + | 306 | WP_053390196 | type IV conjugative transfer system protein TraL | traL |
| KM111_RS00390 | 63895..64461 | + | 567 | WP_053390197 | type IV conjugative transfer system protein TraE | traE |
| KM111_RS00395 | 64448..65182 | + | 735 | WP_053390198 | type-F conjugative transfer system secretin TraK | traK |
| KM111_RS00400 | 65182..66603 | + | 1422 | WP_075606927 | F-type conjugal transfer pilus assembly protein TraB | traB |
| KM111_RS00405 | 66596..67192 | + | 597 | WP_075606926 | conjugal transfer pilus-stabilizing protein TraP | - |
| KM111_RS00410 | 67215..67784 | + | 570 | WP_075606925 | type IV conjugative transfer system lipoprotein TraV | traV |
| KM111_RS00415 | 67896..68174 | + | 279 | WP_053390295 | hypothetical protein | - |
| KM111_RS00420 | 68181..68369 | + | 189 | WP_053390294 | hypothetical protein | - |
| KM111_RS00580 | 68453..68854 | + | 402 | WP_075606924 | hypothetical protein | - |
| KM111_RS00430 | 68851..69150 | + | 300 | WP_053390307 | hypothetical protein | - |
| KM111_RS00435 | 69309..69620 | + | 312 | WP_227524648 | hypothetical protein | - |
| KM111_RS00440 | 69713..72352 | + | 2640 | WP_075606922 | type IV secretion system protein TraC | virb4 |
| KM111_RS00445 | 72352..72741 | + | 390 | WP_060415481 | type-F conjugative transfer system protein TrbI | - |
| KM111_RS00450 | 72738..73364 | + | 627 | WP_060415482 | type-F conjugative transfer system protein TraW | traW |
| KM111_RS00455 | 73408..74367 | + | 960 | WP_229295972 | conjugal transfer pilus assembly protein TraU | traU |
| KM111_RS00460 | 74380..75006 | + | 627 | WP_075606921 | type-F conjugative transfer system pilin assembly protein TrbC | trbC |
| KM111_RS00465 | 75003..76958 | + | 1956 | WP_075606920 | type-F conjugative transfer system mating-pair stabilization protein TraN | traN |
| KM111_RS00470 | 76990..77250 | + | 261 | WP_053390216 | hypothetical protein | - |
| KM111_RS00475 | 77243..77854 | + | 612 | WP_053390217 | hypothetical protein | - |
| KM111_RS00480 | 77844..78086 | + | 243 | WP_053390218 | conjugal transfer protein TrbE | - |
| KM111_RS00485 | 78132..78458 | + | 327 | WP_075606919 | hypothetical protein | - |
| KM111_RS00490 | 78479..79231 | + | 753 | WP_053390220 | type-F conjugative transfer system pilin assembly protein TraF | traF |
| KM111_RS00495 | 79242..79478 | + | 237 | WP_053390221 | type-F conjugative transfer system pilin chaperone TraQ | - |
| KM111_RS00500 | 79453..80013 | + | 561 | WP_039698500 | type-F conjugative transfer system pilin assembly thiol-disulfide isomerase TrbB | traF |
| KM111_RS00505 | 80000..81370 | + | 1371 | WP_075606918 | conjugal transfer pilus assembly protein TraH | traH |
| KM111_RS00510 | 81370..84225 | + | 2856 | WP_075606917 | conjugal transfer mating-pair stabilization protein TraG | traG |
| KM111_RS00515 | 84242..84913 | + | 672 | WP_088883382 | hypothetical protein | - |
| KM111_RS00585 | 85159..85851 | + | 693 | WP_202395549 | hypothetical protein | - |
| KM111_RS00525 | 85970..88288 | + | 2319 | WP_075606915 | type IV conjugative transfer system coupling protein TraD | virb4 |
Host bacterium
| ID | 19303 | GenBank | NZ_MN548433 |
| Plasmid name | pHNNC189-4 | Incompatibility group | IncFII |
| Plasmid size | 99419 bp | Coordinate of oriT [Strand] | 61102..61151 [-] |
| Host baterium | Raoultella ornithinolytica strain NC189 |
Cargo genes
| Drug resistance gene | blaKPC-2 |
| Virulence gene | - |
| Metal resistance gene | - |
| Degradation gene | - |
| Symbiosis gene | - |
| Anti-CRISPR | AcrIE9 |