Detailed information of oriT

oriT

The information of the oriT region

• oriTDB ID: 118711
• Name: oriT_pATF64-2
• Organism: Enterococcus faecium strain ATF64
• Sequence Completeness: -
• NCBI accession of oriT (coordinates [strand]): NZ_JAOXXX010000003 (14437..14474 [-], 38 nt)
• oriT length: 38 nt
• IRs (inverted repeats): 14..22, 29..37 (TAAAGTATA..TATACTTTA) 2..8, 13..19 (ACTTTAT..ATAAAGT)
• Location of nic site: 27..28
• Conserved sequence flanking the nic site: GTGTGTTATA
• Note: Predicted by oriTfinder 2.0

oriT sequence

• Length: 38 nt

>oriT_pATF64-2
CACTTTATGAATATAAAGTATAGTGTGTTATACTTTAC

oriT secondary structure

Predicted by RNAfold.

Relaxase

mobV_OIG04_RS12565_pATF64-2

• ID: 11875
• GenBank: WP_237621712
• Name: mobV_OIG04_RS12565_pATF64-2
• UniProt ID: _
• Length: 420 a.a.
• Note: Predicted by oriTfinder 2.0

Relaxase protein sequence

• Length: 420 a.a.
• Molecular weight: 49642.29 Da
• Isoelectric Point: 9.9693

>WP_237621712.1 MobV family relaxase [Enterococcus faecium]
MSYAVCRMQKVKSAGLKGMQFHNQRERKSRTNDDIDHERTRENYDLKNDKNIDYNERVKEIIESQKTGTR
KTRKDAVLVNELLVTSDRDFFEQLDPGEQKRFFEESYKLFSERYGKQNIAYATVHNDEQTPHMHLGVVPM
RDGKLQGKNVFNRQELLWLQDKFPEHMKKQGFELKRGERGSDRKHIETAKFKKQTLEKEIDFLEKNLAVK
KDEWTAYSDKVKSDLEVPAKRHMKSVEVPTGEKSIFGLGKEIMKTEKKPTKNVVISERDYKNLVTAARDN
DRLKQHVRNLMSTDMAREYKKLSKEHGQVKEKYSGLVERFNENVNDYNELLEENKSLKSKISDLKRDVSL
IYESTKEFLKERTDGLKAFKNVFKGFVDKVKDKTAQFQEKHDLEPKKNEFELTHNREVKKERSRDQGMSL

Protein domains

Predicted by InterproScan.

• Plasmid recombination enzyme (Mob_Pre) (1-193)

Protein structure

No available structure.

Host bacterium

• ID: 19143
• GenBank: NZ_JAOXXX010000003
• Plasmid name: pATF64-2
• Incompatibility group: -
• Plasmid size: 34286 bp
• Coordinate of oriT [Strand]: 14437..14474 [-]
• Host baterium: Enterococcus faecium strain ATF64

Cargo genes

• Drug resistance gene: tet(L), tet(M), erm(B)
• Virulence gene: -
• Metal resistance gene: -
• Degradation gene: -
• Symbiosis gene: -
• Anti-CRISPR: -