Detailed information of oriT

oriT

The information of the oriT region


oriTDB ID   118694
Name   oriT_pFF1003_2 in_silico
Organism   Klebsiella africana strain FF1003
Sequence Completeness      -
NCBI accession of oriT (coordinates [strand])   NZ_CP059393 (18480..18580 [+], 101 nt)
oriT length   101 nt
IRs (inverted repeats)      32..38, 43..49  (TTACGAT..ATCGTAA)
 21..26, 38..43  (TTTTCA..TGAAAA)
Location of nic site      62..63
Conserved sequence flanking the
  nic site    		  GGTGTATAGC
Note   Predicted by oriTfinder 2.0

  oriT sequence  


Download         Length: 101 nt

>oriT_pFF1003_2
AATTAGGATAATTTGTTTTGTTTTCAAGCATTTACGATGAAAATCGTAATTGCGTATGGTGTATAGCCGTTAAGGGATACCATACCACGCCTTTTTTAAGG

Visualization of oriT structure

  oriT secondary structure

Predicted by RNAfold.

Download structure file


Relaxase

ID   11859 GenBank   WP_021536814
Name   mobF_H1X61_RS25905_pFF1003_2 insolico UniProt ID   _
Length   969 a.a. PDB ID   
Note   Predicted by oriTfinder 2.0

  Relaxase protein sequence


Download         Length: 969 a.a.        Molecular weight: 108483.54 Da        Isoelectric Point: 9.7610

>WP_021536814.1 MULTISPECIES: MobF family relaxase [Enterobacterales]
MLNITTITRQRVDKVVDYYADGADDYYAKDGNAMQWQGAGAEELGLTGEVEQKRFAKLLDGKVTDDISVM
RKTANSESKERLGYDLTFSAPKGVTLQALVNGDKRIIEAHDRAVTKAIEEAERLALGRFTVNKKTHVENT
NKLIVGKFRHETSRELDPDLHTHAFVMNLTKTSDGKWRSLTNDGIVNSLTLLGNVYKSELARELELAGYN
LRYDRNGTFDLAHFSQEQIAQFSQRSQQIEAELAKNGLSRETASHEQKNAAALKTRKSKGEVDRDVLYEG
WKNRAKELQIDFDSREWKGAANDDIARNTQPAALEKPIEYQADKVMTFAIRSLTERQSVITERELMDVAM
KHGYGRLTLDDIRAARERAITSGNLIKEEATYAAATTNKKQQNVAMTREQWVTELVKAGRNKDEARKLVD
KGITNGRLVQQKPRFTTQLAQQRERNILKMEREGRGKIQTPYTREFSEGWLASRTLKPEQLKAVMGIIHT
PNQFISVHGFAGTGKSYMTKSAADFLKEQGVHVTSLAPYGSQVKALQAEGLESRTLQSFLRASDKKIGPG
SVVFIDEAGVIPARQMEETMRVIRDAGARAVFLGDTKQTKAVEAGKPFEQLIKAGMETAYMKDIQRQKDP
ELLKAALNAAEGKIKASLTHVTSIVEEKNHDQRYRRIVGDYVAMPPTDRANALIITGTNDSRKKINEYIQ
AELGLKGQGINYPLLNRLDTTQAQRQHSKYYEKGAVIIPERDYSNGLKRGAVYTVLDTGPGNKLTVSDGS
GDTIAFSPARFSKLSVYSVEKTELAVGDQVRITRNDAHLDLANGDRFKVKGVQSGEVLLENEKGRLVKID
ANKPMYLGLAYASTVHSAQGLTCDKVFINMDTRSLTTAKDVYYVAVTRAKHEAVIYTDDEKKLDKAASRE
GFKTAALELEQLKRYAKEQQHDAREKGRDKTEPTQEKDNAKRKGKGHDKNNDERAFTSL

  Protein domains


Predicted by InterproScan.

AAA domain (AAA_30)

(475-649)

UvrD-like helicase C-terminal domain (UvrD_C_2)

(850-896)

TrwC relaxase (TrwC)

(14-287)


  Protein structure



No available structure.




T4CP

ID   13890 GenBank   WP_203034371
Name   t4cp2_H1X61_RS25910_pFF1003_2 insolico UniProt ID   _
Length   525 a.a. PDB ID   _
Note   Predicted by oriTfinder 2.0

  T4CP protein sequence


Download         Length: 525 a.a.        Molecular weight: 58947.78 Da        Isoelectric Point: 9.3727

>WP_203034371.1 type IV secretion system DNA-binding domain-containing protein [Klebsiella africana]
MNEADKGKICLGALLFTPLVTWFIAAKALYGFTPKDARERLVLLIQQTFDLWPLWGALLVGEIIAIIGLI
IFFKFNHQIFKGAPFKKIYRGTRLVSQRALASITKEREPQITIADIPIPTKAEGTHISIAGATGVGKSTI
FAEMMKCCLMRGKMQRVASNKDRMIILDPDGDFLSRFYKKGDKILNPYDARTEGWVFFNEIKSDFDFERF
GVSIVPTAKDSQTEEWNGYGRLLFTEVSRKVFNTSRNPTMEEVFYWTNEVPLEELEDYVRGTKAQALFAG
SGRAVGSARFVLSNKLAAHLKMPAGNFSIREWLEDPKGGNLYITWTDEMREALKSLISCWTDSIFSIVLG
MSKSNSRKIWTFIDELESLDYLPSLRDALTKGRKKGLRVVTGYQSYSQVISIYGSDVAETLLSNHRTSVV
MAAGRLGERTLEFVSKSLGEIEGEREKTGISRRFGQLGTKNTNDDHKRERAVTPTEIATLDDLTGYIAFP
GNLPVAKFETHHVNYTRSNPVPGVVLRESTAFAGM

  Protein domains


Predicted by InterproScan.

Type IV secretion-system coupling protein DNA-binding domain (TrwB_AAD_bind)

(111-499)

  Protein structure



No available structure.




T4SS

T4SS were predicted by using oriTfinder2.

Region 1: 962..18038

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
H1X61_RS25815 (H1X61_25805) 261..986 - 726 WP_228290735 restriction endonuclease -
H1X61_RS25820 (H1X61_25810) 962..1276 - 315 WP_000851068 hypothetical protein tfc15
H1X61_RS25825 (H1X61_25815) 1279..1503 - 225 WP_228290736 TrbM/KikA/MpfK family conjugal transfer protein -
H1X61_RS25830 (H1X61_25820) 1608..2039 - 432 WP_203034359 hypothetical protein -
H1X61_RS25835 (H1X61_25825) 2051..2287 - 237 WP_228290737 H-NS family nucleoid-associated regulatory protein -
H1X61_RS25840 (H1X61_25830) 2349..3089 + 741 WP_203034360 lytic transglycosylase domain-containing protein virB1
H1X61_RS25845 (H1X61_25835) 3112..3429 + 318 WP_203034361 transcriptional regulator -
H1X61_RS25850 (H1X61_25840) 3413..3703 + 291 WP_105446467 TrbC/VirB2 family protein virB2
H1X61_RS25855 (H1X61_25845) 3753..4067 + 315 WP_033544585 type IV secretion system protein VirB3 virB3
H1X61_RS25860 (H1X61_25850) 4067..6697 + 2631 WP_203034362 VirB4 family type IV secretion/conjugal transfer ATPase virb4
H1X61_RS25865 (H1X61_25855) 6707..7414 + 708 WP_203034363 type IV secretion system protein virB5
H1X61_RS25870 (H1X61_25860) 7418..7654 + 237 WP_203034364 EexN family lipoprotein -
H1X61_RS25875 (H1X61_25865) 7673..8719 + 1047 WP_203034365 type IV secretion system protein virB6
H1X61_RS26365 8858..8983 + 126 WP_323128153 hypothetical protein -
H1X61_RS25880 (H1X61_25870) 8986..9702 + 717 WP_203034366 virB8 family protein virB8
H1X61_RS25885 (H1X61_25875) 9707..10594 + 888 WP_203034367 TrbG/VirB9 family P-type conjugative transfer protein virB9
H1X61_RS25890 (H1X61_25880) 10591..11745 + 1155 WP_203034368 type IV secretion system protein VirB10 virB10
H1X61_RS25895 (H1X61_25885) 11738..12769 + 1032 WP_203034369 P-type DNA transfer ATPase VirB11 virB11
H1X61_RS25900 (H1X61_25890) 12732..13286 + 555 WP_203034370 phospholipase D family protein -
H1X61_RS25905 (H1X61_25895) 13549..16458 - 2910 WP_021536814 MobF family relaxase -
H1X61_RS25910 (H1X61_25900) 16461..18038 - 1578 WP_203034371 type IV secretion system DNA-binding domain-containing protein virb4
H1X61_RS25915 (H1X61_25905) 18031..18378 - 348 WP_001139329 hypothetical protein -
H1X61_RS25920 (H1X61_25910) 18854..19255 + 402 WP_203034353 hypothetical protein -
H1X61_RS25925 (H1X61_25915) 19268..19978 + 711 WP_021544361 StbB family protein -
H1X61_RS25930 (H1X61_25920) 19980..20348 + 369 WP_015345231 plasmid stabilization protein StbC -
H1X61_RS25935 (H1X61_25925) 20562..21332 + 771 WP_000586559 SprT-like domain-containing protein -
H1X61_RS25940 (H1X61_25930) 21374..21562 - 189 WP_001001010 hypothetical protein -
H1X61_RS25945 (H1X61_25935) 21567..21824 - 258 WP_021536820 hypothetical protein -
H1X61_RS25950 (H1X61_25940) 21897..22052 - 156 WP_021536821 hypothetical protein -
H1X61_RS25955 (H1X61_25945) 22204..22491 - 288 WP_001098466 hypothetical protein -

Host bacterium

ID   19126 GenBank   NZ_CP059393
Plasmid name   pFF1003_2 Incompatibility group   -
Plasmid size   33201 bp Coordinate of oriT [Strand]   18480..18580 [+]
Host baterium   Klebsiella africana strain FF1003

Cargo genes

Drug resistance gene   -
Virulence gene   -
Metal resistance gene   -
Degradation gene   -
Symbiosis gene   -
Anti-CRISPR   -