Detailed information of oriT

oriT

The information of the oriT region

• oriTDB ID: 118629
• Name: oriT_pIMPIncN3_52kb
• Organism: Enterobacter hormaechei strain EB_P6_L3_02.19
• Sequence Completeness: -
• NCBI accession of oriT (coordinates [strand]): NZ_CP043856 (51370..51459 [+], 90 nt)
• oriT length: 90 nt
• IRs (inverted repeats): 34..41, 44..51 (AGTGCGAT..ATCGCACT)
• Location of nic site: 62..63
• Conserved sequence flanking the nic site: GGTGTATAGC
• Note: Predicted by oriTfinder 2.0

oriT sequence

• Length: 90 nt

>oriT_pIMPIncN3_52kb
TATTTCATTTTTTGTTTTTTTAATCAGTTAGTTAGTGCGATTCATCGCACTGCGTTAGGTGTATAGCAGGTTAAGGGCTAAAAAATAATC

oriT secondary structure

Predicted by RNAfold.

Relaxase

mobF_F1867_RS26410_pIMPIncN3_52kb

• ID: 11812
• GenBank: WP_024561907
• Name: mobF_F1867_RS26410_pIMPIncN3_52kb
• UniProt ID: H1ZMV6
• Length: 1072 a.a.
• Note: Predicted by oriTfinder 2.0

Relaxase protein sequence

• Length: 1072 a.a.
• Molecular weight: 118896.59 Da
• Isoelectric Point: 6.4636

>WP_024561907.1 MULTISPECIES: MobF family relaxase [Enterobacterales]
MLDITTITRIDIASVVGYYADAKDDYYSKDSSFTAWHGQGAEALGLSGEVSSQRFKELLVGEIDPFTQMK
RSSGDATKERLGYDLTFSAPKGVSMQALIHGDKAIIAAHEKAVAAAVQEAEKLAQARTTHAGKTITQNTG
NLVVASFRHETSRALDPELHTHAFVMNMTQRGDGQWRALKNDELMRAKMHLGDVYKQELALELTKAGYEL
RYNNKNNTFDMAHFTDEQIRGFSRRSAQIEEGLAAMGLTRETADAQTKSRVSMATRDKKTDDYSREDIHK
EWVNRANELGIDFNDLSWAGKGKDGPQTSMNTVPNFTSPEVKADRAIQFALKSLSERDASFDQSRLLAVA
NKKAIGQASKQDIEAAYYRAVAKGAIIEGEARYTTTTDQRSKDAPPLPAMTKAEWQAQLTDKKGMNNDQA
KALVAEGIKSGRLKKTSHRVTTVEGIRVEKAVLNIEARGRGKVIAALTSEQVTAQLVGKTLKAEQQKSVV
DICTTTDRFVAAHGFAGTGKSYMTMSAKAVLESQGFNVTALAPYGTQKKSLEDEGMPARTVAAFVKAKDK
KIDEKSVVFIDEAGVIPARQMKVLMETIEKAGARAVFLGDTSQTKAVEAGKPFEQLISAGMQTSYMKDIQ
RQKNDVLLEAVKLAAEGHISGSLARLSNISAEKDQDKRLEAVANRWLSFSPEQREGTLIISGTNESRVIL
NSQIREALQLQGKGVEVNFLERVDSTQAERRDSKYYQVGQIIIPEKDYKNGLQRGESYRVLDTGPGNLLT
VAGSDGQKISFSPRTHKNLSVYTSVAAELAVGDKVMVTRNDKTLDVANGDRFTVTGTTEKGTFTLTNAKG
REIELDQKQAAYLSYAYATTVHKAQGLTCDRVLFNIDTRSLTTSKDVFYVGISRARHEVEIFTDDKKRLL
SSASRNSPKTTASEIDRFLGMEERYKDVNRSYGHSDGQQEKSYGHDAGHQTEEKENTMKNATEAAHDGPD
TSGTENTSQDLRQREQEEVNAAIRYEQRQEATPEMEANPYHDYERYADEGPDYGDMQDYSTYEEYERARD
VELPQHEHENHQHDHDKEGYGL

Protein domains

Predicted by InterproScan.

• TrwC relaxase (TrwC) (16-288)

• AAA domain (AAA_30) (480-656)

• UvrD-like helicase C-terminal domain (UvrD_C_2) (855-899)

Protein structure

• AlphaFold DB: H1ZMV6

T4CP

t4cp2_F1867_RS26415_pIMPIncN3_52kb

• ID: 13840
• GenBank: WP_032493179
• Name: t4cp2_F1867_RS26415_pIMPIncN3_52kb
• UniProt ID: _
• Length: 510 a.a.
• PDB ID: _
• Note: Predicted by oriTfinder 2.0

T4CP protein sequence

• Length: 510 a.a.
• Molecular weight: 57912.96 Da
• Isoelectric Point: 9.5127

>WP_032493179.1 MULTISPECIES: type IV secretion system DNA-binding domain-containing protein [Enterobacterales]
MTEQERGLIFLCSLLGPIPFVWFITAKFVYGINSETAHYLVPYLLKNTFNLWPLWVAIIAGAVIGLCGFI
GFIIYDKGRVYKGPRFKRVLRGTKLVRASSLAEKTKERDHKQLTVAGVPVPTEYETLHFSIGGATGTGKS
TIIKEAIYSSLKRGTDKRIVLDPNGEYVETFYRPGDVILSPYDKRSPGWSMFNEIRDDFDYDLYAVSIIQ
ESKERSAEEWYRYGRLIYREVSKKLLTLKRSPTMEEVMHWCTIVENEKLKEFVKGTPAEALFIKGAERAT
GSARFVLGDKLAPHLKMPAGDFSLRDWLEDDKPGTLFITWQEQMKKSLNPLISCWLDIMFSSLLGMGKRD
QRTLFFIDELESLEYLPNLQDLLTKGRKSGASVWAGYQTYSQLIERYGINIAETLLGSMRSGIVMGSSRL
GKETLEQMSRALGEIEGEVEHKQGNPFQAGIGNRPRIETRTVRAVTPTEISELKNLTGYVYFPGDLPVGK
FKTKHVQYTRKNPVPGIIMR

Protein domains

Predicted by InterproScan.

• Type IV secretion-system coupling protein DNA-binding domain (TrwB_AAD_bind) (113-492)

Protein structure

No available structure.

T4SS

T4SS were predicted by using oriTfinder2.

Region 1: 16596..26872

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
F1867_RS26165 (F1867_26165)	13585..13917	+	333	WP_032493174	hypothetical protein	-
F1867_RS26170 (F1867_26170)	14104..14712	+	609	WP_000275218	recombinase family protein	-
F1867_RS26175 (F1867_26175)	14709..14894	-	186	WP_024562121	ribbon-helix-helix domain-containing protein	-
F1867_RS26180 (F1867_26180)	15178..15486	-	309	WP_000733764	hypothetical protein	-
F1867_RS26185 (F1867_26185)	15490..15828	-	339	WP_032493175	hypothetical protein	-
F1867_RS26190 (F1867_26190)	15825..16175	-	351	WP_024562120	hypothetical protein	-
F1867_RS26195 (F1867_26195)	16179..16496	-	318	WP_000960955	H-NS family nucleoid-associated regulatory protein	-
F1867_RS26200 (F1867_26200)	16596..17378	+	783	WP_024562119	lytic transglycosylase domain-containing protein	virB1
F1867_RS26210 (F1867_26210)	17625..17921	+	297	WP_015060075	TrbC/VirB2 family protein	virB2
F1867_RS26215 (F1867_26215)	17961..18278	+	318	WP_000462629	type IV secretion system protein VirB3	virB3
F1867_RS26220 (F1867_26220)	18279..20852	+	2574	WP_040115085	VirB4 family type IV secretion/conjugal transfer ATPase	virb4
F1867_RS26225 (F1867_26225)	20868..21569	+	702	WP_014014949	type IV secretion system protein	virB5
F1867_RS26230 (F1867_26230)	21579..21821	+	243	WP_000858958	EexN family lipoprotein	-
F1867_RS26235 (F1867_26235)	21838..22875	+	1038	WP_014014948	type IV secretion system protein	virB6
F1867_RS27075 (F1867_26240)	22952..23083	+	132	WP_032493177	hypothetical protein	-
F1867_RS26245 (F1867_26245)	23094..23789	+	696	WP_001208352	type IV secretion system protein	virB8
F1867_RS26250 (F1867_26250)	23800..24687	+	888	WP_000758230	TrbG/VirB9 family P-type conjugative transfer protein	virB9
F1867_RS26255 (F1867_26255)	24687..25820	+	1134	WP_000101920	type IV secretion system protein VirB10	virB10
F1867_RS26260 (F1867_26260)	25871..26872	+	1002	WP_001076634	ATPase, T2SS/T4P/T4SS family	virB11
F1867_RS26265 (F1867_26265)	26884..27396	+	513	WP_029697418	phospholipase D family protein	-
F1867_RS26270 (F1867_26270)	27905..28912	-	1008	WP_149912353	IS110-like element IS4321 family transposase	-

Host bacterium

• ID: 19061
• GenBank: NZ_CP043856
• Plasmid name: pIMPIncN3_52kb
• Incompatibility group: IncN3
• Plasmid size: 51628 bp
• Coordinate of oriT [Strand]: 51370..51459 [+]
• Host baterium: Enterobacter hormaechei strain EB_P6_L3_02.19

Cargo genes

• Drug resistance gene: blaIMP-70, qacE, sul1
• Virulence gene: -
• Metal resistance gene: merE, merD, merA, merC, merP, merT, merR
• Degradation gene: -
• Symbiosis gene: -
• Anti-CRISPR: -