Detailed information of oriT
oriT
The information of the oriT region
| oriTDB ID | 118624 |
| Name | oriT_plamid_2  |
| Organism | Klebsiella grimontii strain SS141 |
| Sequence Completeness | - |
| NCBI accession of oriT (coordinates [strand]) | NZ_CP044529 (75237..75286 [+], 50 nt) |
| oriT length | 50 nt |
| IRs (inverted repeats) | 7..14, 17..24 (GCAAAATT..AATTTTGC) |
| Location of nic site | _ |
| Conserved sequence flanking the nic site |
_ |
| Note | Predicted by oriTfinder 2.0 |
oriT sequence
Download Length: 50 nt
>oriT_plamid_2
AAATCTGCAAAATTTTAATTTTGCGTGGGGTGTGGTCATTTTGTGGTGAG
AAATCTGCAAAATTTTAATTTTGCGTGGGGTGTGGTCATTTTGTGGTGAG
Visualization of oriT structure
oriT secondary structure
Predicted by RNAfold.
Download structure file
Relaxase
| ID | 11809 | GenBank | WP_064353420 |
| Name | traI_EHW69_RS29855_plamid_2 |
UniProt ID | _ |
| Length | 1753 a.a. | PDB ID | |
| Note | Predicted by oriTfinder 2.0 | ||
Relaxase protein sequence
Download Length: 1753 a.a. Molecular weight: 190811.84 Da Isoelectric Point: 6.1732
>WP_064353420.1 MULTISPECIES: conjugative transfer relaxase/helicase TraI [Klebsiella]
MMSIGSVKSAGSAGNYYTDKDNYYVIGSMDERWQGKGAEALGIDGKAVDKALFTELLKGKLPDGSDLTRI
QDGANRHRPGYDLTFSAPKSVSVMAMLGGDKRLIDAHNQAVTEAVRQLETLAATRVMTDGKSETVLTGNL
IVAKFNHDTNRNQEPQIHTHAVVINATQNGDKWQSLGTDKIGKTGFIENVYANQIAFGKLYREAFKPLVE
KLGYETEVVGKHGMWEMKGVPVEPFSTRSQEVREAAGPDASLKSRDVAALDTRKSKEAIDPAEKMVEWMN
TLKETGFDIRGYREAADARAAELARAPAAPVNTDGPDITDVVTKAIAGLSDRKVQFTYADLLARTVGQLE
AKDGVFDLARKGIDAAIEREQLIPLDREKGLFTSNIHVLDELAVKALSQEVQRQNHVSVTPDASVVRQVP
FSDAVSVLAQDRLVMGIVSGQGGATGQRERVAELTLMAREQGRDVHILAADNRSRDFLAGDVRLAGETVT
GKSALQDGTAFIPGGTLIVDQAEKLSLKETISLLDGAMRHNVQVLLSDSGKRSGTGSALTVLKDSGVNTY
RWQGGQQTTADIISEPDKGARYSRLAQEFAVSVREGQESVAQISGTREQSVLNGLIRDSLRQEGVLGEKD
TTITALTPVWLDSKSRGVRDYYREGMVMERWDPENRTHDRFVIDRVTASSNMLTLKDRDGVRLDLKVSAV
DSQWTLFRAETLPVAEGERLAVLGKIPDTRLKGGESITVMKVEEGQLTVQRPGQKTTQTLAVGAGVFDGI
KIGHGWVESPGRSVSETATVFASVTQRELDNATLNQLAQSGSHLRLYSAQDAARTTEKLSRHTAFSVVSE
QLKSRSGETDLDAAIAQQKAGLHTPAEQAIHLAIPLLESQDLTFSRPQLLATAMETGGGKVSMADIDTTI
QAQIRSGQLLNVPVAHGYGNDLLISRQTWDAEKSILTRVLEGKDAVAPLMDRVPASLMTDLTAGQRAATR
MILESTDRFTVVQGYAGVGKTTQFRAVMSAISLLPEETRPRVIGLAPTHRAVGEMQSAGVDARTTASFLH
DTQLLQRNGQTPDFSNTLFLLDESSMVGLADMAKAHSLIVAGGGRAVSSGDNDQLQPIAPGQPFRLMQQR
SAADVAIMKEIVRQMPELRPAVYSLIERDVHRALTTIEQVTPEQVPRKEGSWAPGSSVVEFTPKQEKAIE
KALSEGKTLPEGQPATLYEALVKDYTGRTPEAQSQTLVITHLNKDRRALNSLIHDARRENGETGKEEITL
PVLVTSNIRDGELRKLSTWTAHKEAVALVDNVYHRISKVDKDNQLITLTDSEGKERFISPREASAEGVTL
YRQEKITVSQGDRMRFSKSDPERGYVANSIWEVQSVSGDSVTLSDGKITRTLTPKAEQAQQHIDLAYAIT
AHGAQGASEPYAIALEGVAGGREQMASFESAYVALSRMKQHVQVYTDNREGWIKAIKNSPEKATAHDILE
PRNDRAVKTADLLFGRARPLDETAAGRAALQQSGLAQGSSPGKFISPGKKYPQPHVALPAFDKNGKAAGI
WLSPLTDRDGRLEAIGGEGRIMGNEDARFVALQNSRNGESLLAGNMGEGVRMARDNPDTGVVVRLAGDDR
PWNPGAMTGGRVWAEPAPVAPVPQAGADIILPPEVLAQRAAEEQQRREMEKQAEQTAREVAGEARKAGEP
ADRVKEVIGDVIRGLERDRPGTEKTTLPDDPQFRRQEEAIQQVASERLQRERLQAVERDMVRDLNREKTL
GGD
MMSIGSVKSAGSAGNYYTDKDNYYVIGSMDERWQGKGAEALGIDGKAVDKALFTELLKGKLPDGSDLTRI
QDGANRHRPGYDLTFSAPKSVSVMAMLGGDKRLIDAHNQAVTEAVRQLETLAATRVMTDGKSETVLTGNL
IVAKFNHDTNRNQEPQIHTHAVVINATQNGDKWQSLGTDKIGKTGFIENVYANQIAFGKLYREAFKPLVE
KLGYETEVVGKHGMWEMKGVPVEPFSTRSQEVREAAGPDASLKSRDVAALDTRKSKEAIDPAEKMVEWMN
TLKETGFDIRGYREAADARAAELARAPAAPVNTDGPDITDVVTKAIAGLSDRKVQFTYADLLARTVGQLE
AKDGVFDLARKGIDAAIEREQLIPLDREKGLFTSNIHVLDELAVKALSQEVQRQNHVSVTPDASVVRQVP
FSDAVSVLAQDRLVMGIVSGQGGATGQRERVAELTLMAREQGRDVHILAADNRSRDFLAGDVRLAGETVT
GKSALQDGTAFIPGGTLIVDQAEKLSLKETISLLDGAMRHNVQVLLSDSGKRSGTGSALTVLKDSGVNTY
RWQGGQQTTADIISEPDKGARYSRLAQEFAVSVREGQESVAQISGTREQSVLNGLIRDSLRQEGVLGEKD
TTITALTPVWLDSKSRGVRDYYREGMVMERWDPENRTHDRFVIDRVTASSNMLTLKDRDGVRLDLKVSAV
DSQWTLFRAETLPVAEGERLAVLGKIPDTRLKGGESITVMKVEEGQLTVQRPGQKTTQTLAVGAGVFDGI
KIGHGWVESPGRSVSETATVFASVTQRELDNATLNQLAQSGSHLRLYSAQDAARTTEKLSRHTAFSVVSE
QLKSRSGETDLDAAIAQQKAGLHTPAEQAIHLAIPLLESQDLTFSRPQLLATAMETGGGKVSMADIDTTI
QAQIRSGQLLNVPVAHGYGNDLLISRQTWDAEKSILTRVLEGKDAVAPLMDRVPASLMTDLTAGQRAATR
MILESTDRFTVVQGYAGVGKTTQFRAVMSAISLLPEETRPRVIGLAPTHRAVGEMQSAGVDARTTASFLH
DTQLLQRNGQTPDFSNTLFLLDESSMVGLADMAKAHSLIVAGGGRAVSSGDNDQLQPIAPGQPFRLMQQR
SAADVAIMKEIVRQMPELRPAVYSLIERDVHRALTTIEQVTPEQVPRKEGSWAPGSSVVEFTPKQEKAIE
KALSEGKTLPEGQPATLYEALVKDYTGRTPEAQSQTLVITHLNKDRRALNSLIHDARRENGETGKEEITL
PVLVTSNIRDGELRKLSTWTAHKEAVALVDNVYHRISKVDKDNQLITLTDSEGKERFISPREASAEGVTL
YRQEKITVSQGDRMRFSKSDPERGYVANSIWEVQSVSGDSVTLSDGKITRTLTPKAEQAQQHIDLAYAIT
AHGAQGASEPYAIALEGVAGGREQMASFESAYVALSRMKQHVQVYTDNREGWIKAIKNSPEKATAHDILE
PRNDRAVKTADLLFGRARPLDETAAGRAALQQSGLAQGSSPGKFISPGKKYPQPHVALPAFDKNGKAAGI
WLSPLTDRDGRLEAIGGEGRIMGNEDARFVALQNSRNGESLLAGNMGEGVRMARDNPDTGVVVRLAGDDR
PWNPGAMTGGRVWAEPAPVAPVPQAGADIILPPEVLAQRAAEEQQRREMEKQAEQTAREVAGEARKAGEP
ADRVKEVIGDVIRGLERDRPGTEKTTLPDDPQFRRQEEAIQQVASERLQRERLQAVERDMVRDLNREKTL
GGD
Protein domains
Predicted by InterproScan.
Protein structure
No available structure.
T4CP
| ID | 13836 | GenBank | WP_048235023 |
| Name | traD_EHW69_RS29860_plamid_2 |
UniProt ID | _ |
| Length | 770 a.a. | PDB ID | _ |
| Note | Predicted by oriTfinder 2.0 | ||
T4CP protein sequence
Download Length: 770 a.a. Molecular weight: 85941.02 Da Isoelectric Point: 5.1149
>WP_048235023.1 MULTISPECIES: type IV conjugative transfer system coupling protein TraD [Enterobacterales]
MSFNAKDMTQGGQIANMRFRMFGQIANIIFYVLFILFWVLCGLMLMYRLSWQTFVNGCVYWWCTTLGPMR
DIIRSQPVYTIQYYGQSLEYTSEQILADKYTIWCGEQLWTSFVFAAVVSLVICIVTFFIASWVLGRQGKQ
QSEDENTGGRQLSDKPKEVARQMKRDGMASDIKIGDLPILKNSEIQNFCLHGTVGSGKSEVIRRLLNYVR
ARGDMAIIYDRSCEFVKSYYDPSLDKILNPLDSRCAAWDLWKECLTLPDFDNISNTLIPMGTKEDPFWQG
SGRTIFAEGAYLMREDKDRSYEKLVDTMLSIKIDKLRAYLQNTPAANLVEEKIEKTAISIRAVLTNYVKA
IRYLQGIEKNGEPFTIRDWMRGVREDRPNGWLFISSNADTHASLKPVISMWLSIAIRGLLAMGENRNRRV
WIFADELPTLHKLPDLVEILPEARKFGGCYVFGIQSYAQLEDIYGVKPAATLFDVMNTRAFFRSPSKEIA
EFAAGEIGEKEILKASEQYSYGADPVRDGVSTGKEKERETLVSYSDIQTLPDLSCYVTLPGPYPAVKLAL
KYKPRPKIAEGFIPRTLDARVDARLSALLEAREAEGSLARALFTPDAPASGPADTNSHAGEQPEPVSQPA
PADMTVSPAPVKAPPTTKMPAEEPSVRSTEPSALRLTTVPLIKPKAAAAAAAAATVSSAGTPAAAAGGTE
QELAQQSAEQGQDMLPAGMNEDGVIEDMQAYDAWLADEQTQRDMQRREEVNINHSHRHDEQDNVEIGGNF
MSFNAKDMTQGGQIANMRFRMFGQIANIIFYVLFILFWVLCGLMLMYRLSWQTFVNGCVYWWCTTLGPMR
DIIRSQPVYTIQYYGQSLEYTSEQILADKYTIWCGEQLWTSFVFAAVVSLVICIVTFFIASWVLGRQGKQ
QSEDENTGGRQLSDKPKEVARQMKRDGMASDIKIGDLPILKNSEIQNFCLHGTVGSGKSEVIRRLLNYVR
ARGDMAIIYDRSCEFVKSYYDPSLDKILNPLDSRCAAWDLWKECLTLPDFDNISNTLIPMGTKEDPFWQG
SGRTIFAEGAYLMREDKDRSYEKLVDTMLSIKIDKLRAYLQNTPAANLVEEKIEKTAISIRAVLTNYVKA
IRYLQGIEKNGEPFTIRDWMRGVREDRPNGWLFISSNADTHASLKPVISMWLSIAIRGLLAMGENRNRRV
WIFADELPTLHKLPDLVEILPEARKFGGCYVFGIQSYAQLEDIYGVKPAATLFDVMNTRAFFRSPSKEIA
EFAAGEIGEKEILKASEQYSYGADPVRDGVSTGKEKERETLVSYSDIQTLPDLSCYVTLPGPYPAVKLAL
KYKPRPKIAEGFIPRTLDARVDARLSALLEAREAEGSLARALFTPDAPASGPADTNSHAGEQPEPVSQPA
PADMTVSPAPVKAPPTTKMPAEEPSVRSTEPSALRLTTVPLIKPKAAAAAAAAATVSSAGTPAAAAGGTE
QELAQQSAEQGQDMLPAGMNEDGVIEDMQAYDAWLADEQTQRDMQRREEVNINHSHRHDEQDNVEIGGNF
Protein domains
Predicted by InterproScan.
Protein structure
No available structure.
T4SS
T4SS were predicted by using oriTfinder2.
Region 1: 47233..75844
| Locus tag | Coordinates | Strand | Size (bp) | Protein ID | Product | Description |
|---|---|---|---|---|---|---|
| EHW69_RS29860 | 47233..49545 | - | 2313 | WP_048235023 | type IV conjugative transfer system coupling protein TraD | virb4 |
| EHW69_RS31005 | 49601..49714 | - | 114 | Protein_49 | hypothetical protein | - |
| EHW69_RS29865 | 49905..50636 | - | 732 | WP_032415736 | conjugal transfer complement resistance protein TraT | - |
| EHW69_RS29870 | 50888..51490 | - | 603 | WP_023280878 | hypothetical protein | - |
| EHW69_RS29875 | 51514..54336 | - | 2823 | WP_023320108 | conjugal transfer mating-pair stabilization protein TraG | traG |
| EHW69_RS29880 | 54336..55715 | - | 1380 | WP_011977731 | conjugal transfer pilus assembly protein TraH | traH |
| EHW69_RS29885 | 55693..56136 | - | 444 | WP_032436741 | F-type conjugal transfer protein TrbF | - |
| EHW69_RS29890 | 56181..56738 | - | 558 | WP_004152678 | type-F conjugative transfer system pilin assembly thiol-disulfide isomerase TrbB | traF |
| EHW69_RS29895 | 56710..56949 | - | 240 | WP_004144400 | type-F conjugative transfer system pilin chaperone TraQ | - |
| EHW69_RS29900 | 56960..57712 | - | 753 | WP_015065637 | type-F conjugative transfer system pilin assembly protein TraF | traF |
| EHW69_RS29905 | 57733..58059 | - | 327 | WP_032436744 | hypothetical protein | - |
| EHW69_RS31155 | 58105..58290 | - | 186 | WP_032436745 | hypothetical protein | - |
| EHW69_RS29915 | 58287..58523 | - | 237 | WP_032436746 | conjugal transfer protein TrbE | - |
| EHW69_RS29920 | 58555..60510 | - | 1956 | WP_023307537 | type-F conjugative transfer system mating-pair stabilization protein TraN | traN |
| EHW69_RS29925 | 60569..61207 | - | 639 | WP_015065635 | type-F conjugative transfer system pilin assembly protein TrbC | trbC |
| EHW69_RS29930 | 61220..62179 | - | 960 | WP_015065634 | conjugal transfer pilus assembly protein TraU | traU |
| EHW69_RS29935 | 62223..62849 | - | 627 | WP_023307538 | type-F conjugative transfer system protein TraW | traW |
| EHW69_RS29940 | 62849..63238 | - | 390 | WP_032436749 | type-F conjugative transfer system protein TrbI | - |
| EHW69_RS29945 | 63238..65877 | - | 2640 | WP_016528865 | type IV secretion system protein TraC | virb4 |
| EHW69_RS29950 | 65949..66347 | - | 399 | WP_072158599 | hypothetical protein | - |
| EHW69_RS29955 | 66355..66645 | - | 291 | WP_023307542 | hypothetical protein | - |
| EHW69_RS29960 | 66642..67046 | - | 405 | WP_023307543 | hypothetical protein | - |
| EHW69_RS29965 | 67113..67430 | - | 318 | WP_023307544 | hypothetical protein | - |
| EHW69_RS29970 | 67431..67649 | - | 219 | WP_004171484 | hypothetical protein | - |
| EHW69_RS29975 | 67673..67963 | - | 291 | WP_004152596 | hypothetical protein | - |
| EHW69_RS29980 | 67968..68378 | - | 411 | WP_004152597 | lipase chaperone | - |
| EHW69_RS29985 | 68510..69079 | - | 570 | WP_074186487 | type IV conjugative transfer system lipoprotein TraV | traV |
| EHW69_RS29990 | 69102..69698 | - | 597 | WP_004152599 | conjugal transfer pilus-stabilizing protein TraP | - |
| EHW69_RS29995 | 69691..71115 | - | 1425 | WP_004152600 | F-type conjugal transfer pilus assembly protein TraB | traB |
| EHW69_RS30000 | 71115..71855 | - | 741 | WP_004152601 | type-F conjugative transfer system secretin TraK | traK |
| EHW69_RS30005 | 71842..72408 | - | 567 | WP_004152602 | type IV conjugative transfer system protein TraE | traE |
| EHW69_RS30010 | 72428..72733 | - | 306 | WP_004178059 | type IV conjugative transfer system protein TraL | traL |
| EHW69_RS30015 | 72747..73115 | - | 369 | WP_004178060 | type IV conjugative transfer system pilin TraA | - |
| EHW69_RS30020 | 73169..73540 | - | 372 | WP_004208838 | TraY domain-containing protein | - |
| EHW69_RS30025 | 73624..74319 | - | 696 | WP_004178061 | transcriptional regulator TraJ family protein | - |
| EHW69_RS30030 | 74533..74925 | - | 393 | WP_004178062 | conjugal transfer relaxosome DNA-binding protein TraM | - |
| EHW69_RS30035 | 75359..75844 | + | 486 | WP_004178063 | transglycosylase SLT domain-containing protein | virB1 |
| EHW69_RS30040 | 75877..76206 | - | 330 | WP_011977736 | DUF5983 family protein | - |
| EHW69_RS30045 | 76239..77060 | - | 822 | WP_004178064 | DUF932 domain-containing protein | - |
| EHW69_RS30065 | 77894..78307 | - | 414 | WP_004152722 | type II toxin-antitoxin system HigA family antitoxin | - |
| EHW69_RS30070 | 78308..78586 | - | 279 | WP_004152721 | helix-turn-helix transcriptional regulator | - |
| EHW69_RS30075 | 78576..78896 | - | 321 | WP_004152720 | type II toxin-antitoxin system RelE/ParE family toxin | - |
| EHW69_RS30080 | 78977..79201 | - | 225 | WP_004152719 | hypothetical protein | - |
| EHW69_RS30085 | 79212..79424 | - | 213 | WP_004152718 | hypothetical protein | - |
| EHW69_RS30090 | 79485..79841 | - | 357 | WP_004152717 | hypothetical protein | - |
| EHW69_RS30100 | 80477..80827 | - | 351 | WP_004182068 | hypothetical protein | - |
Host bacterium
| ID | 19057 | GenBank | NZ_CP044529 |
| Plasmid name | plamid_2 | Incompatibility group | IncFII |
| Plasmid size | 100742 bp | Coordinate of oriT [Strand] | 75237..75286 [+] |
| Host baterium | Klebsiella grimontii strain SS141 |
Cargo genes
| Drug resistance gene | - |
| Virulence gene | - |
| Metal resistance gene | - |
| Degradation gene | - |
| Symbiosis gene | - |
| Anti-CRISPR | AcrIE9 |