Detailed information of oriT
oriT
The information of the oriT region
| oriTDB ID | 118532 |
| Name | oriT_pDA33145-77  |
| Organism | Klebsiella quasipneumoniae subsp. similipneumoniae strain ATCC 700603 |
| Sequence Completeness | - |
| NCBI accession of oriT (coordinates [strand]) | NZ_CP029599 (75405..75509 [+], 105 nt) |
| oriT length | 105 nt |
| IRs (inverted repeats) | 32..37, 40..45 (GCCGGC..GCCGGC) |
| Location of nic site | _ |
| Conserved sequence flanking the nic site |
_ |
| Note | Predicted by oriTfinder 2.0 |
oriT sequence
Download Length: 105 nt
>oriT_pDA33145-77
AGTACGGGACAAGATGTGTTTTTGGTACACCGCCGGCACGCCGGCAGTGACGCAAAAACGTCTTGGTCAGGGCAAGCCCCGACACCCCCTAACGAGGTTAGCTAT
AGTACGGGACAAGATGTGTTTTTGGTACACCGCCGGCACGCCGGCAGTGACGCAAAAACGTCTTGGTCAGGGCAAGCCCCGACACCCCCTAACGAGGTTAGCTAT
Visualization of oriT structure
oriT secondary structure
Predicted by RNAfold.
Download structure file
Relaxase
| ID | 11764 | GenBank | WP_004206924 |
| Name | traI_DLJ73_RS27380_pDA33145-77 |
UniProt ID | A0A2V4FJ40 |
| Length | 659 a.a. | PDB ID | |
| Note | Predicted by oriTfinder 2.0 | ||
Relaxase protein sequence
Download Length: 659 a.a. Molecular weight: 75087.84 Da Isoelectric Point: 10.0285
>WP_004206924.1 MULTISPECIES: TraI/MobA(P) family conjugative relaxase [Enterobacterales]
MVIPKIIEGRRDKKSSFGQLIKYMADKPSQELTDTVQPTPETALAVKSDMFEGLNNYLTRKQKVISTPVD
VEPGVQRVVVGDVTCQYNTFSLDGAAQEMNSVSQQSTRCKDPVMHYVLSWPDYEKPNDDQVFDSVKFTLA
SMGMSDHQYVAAIHRDTDNLHVHVAVNRINPQTYKAASSSFTKDTLHQACRLLELKNGWSHSNGAYVVND
RQQIVRNPHSKKERGNWRSLDRINKMENKEGVETLYRYIVGDEQVGGSRQNLIHVSAGLREAKSWDDVHK
TFAGIGLRVEKAQGKKGYVITHEHQNQKTAVKASLVFNKAQYTLKSMEERFGEYQPSHIEPAKVSVFKTA
YTPGAYRRDANKRLQRKIERAEERMLLKGRYRAYRNNLPIYSPDKDRIADEYRKIAQHTRLVKNNVRHSV
SDPHTRKLMYNLAEFKRLQAVANLRLSLREERNGFRAANPRLSYREWVEQEALKGDKAALSQMRGFAYSS
RKKEKYKQQLVEQIGFNRTFNAITSHDRDDVAVMASARHGVKPRLLKDGTVIFERDGKPVAADRGHIVLT
ESNGIDKEKTADLAIALTIAGKAKSVRVDGDGEFKELCCNRIVDAAVNHNHPVAQGITFTDAAQQAYAQN
EKHRLIREQNNSKNEMQLRSESDDKFNPK
MVIPKIIEGRRDKKSSFGQLIKYMADKPSQELTDTVQPTPETALAVKSDMFEGLNNYLTRKQKVISTPVD
VEPGVQRVVVGDVTCQYNTFSLDGAAQEMNSVSQQSTRCKDPVMHYVLSWPDYEKPNDDQVFDSVKFTLA
SMGMSDHQYVAAIHRDTDNLHVHVAVNRINPQTYKAASSSFTKDTLHQACRLLELKNGWSHSNGAYVVND
RQQIVRNPHSKKERGNWRSLDRINKMENKEGVETLYRYIVGDEQVGGSRQNLIHVSAGLREAKSWDDVHK
TFAGIGLRVEKAQGKKGYVITHEHQNQKTAVKASLVFNKAQYTLKSMEERFGEYQPSHIEPAKVSVFKTA
YTPGAYRRDANKRLQRKIERAEERMLLKGRYRAYRNNLPIYSPDKDRIADEYRKIAQHTRLVKNNVRHSV
SDPHTRKLMYNLAEFKRLQAVANLRLSLREERNGFRAANPRLSYREWVEQEALKGDKAALSQMRGFAYSS
RKKEKYKQQLVEQIGFNRTFNAITSHDRDDVAVMASARHGVKPRLLKDGTVIFERDGKPVAADRGHIVLT
ESNGIDKEKTADLAIALTIAGKAKSVRVDGDGEFKELCCNRIVDAAVNHNHPVAQGITFTDAAQQAYAQN
EKHRLIREQNNSKNEMQLRSESDDKFNPK
Protein domains
Predicted by InterproScan.
Protein structure
| Source | ID | Structure |
|---|---|---|
| AlphaFold DB | A0A2V4FJ40 |
T4CP
| ID | 13779 | GenBank | WP_004206885 |
| Name | t4cp2_DLJ73_RS27645_pDA33145-77 |
UniProt ID | _ |
| Length | 695 a.a. | PDB ID | _ |
| Note | Predicted by oriTfinder 2.0 | ||
T4CP protein sequence
Download Length: 695 a.a. Molecular weight: 79538.91 Da Isoelectric Point: 4.8317
>WP_004206885.1 MULTISPECIES: conjugal transfer protein TrbC [Enterobacterales]
MQQESVDSKKVLRPDGSFMEWFLTPNVQFGLLAILTVLGLFLPFTMLLSILILPPLMVAFTDRKFRPPLR
MPKDCEMFDDTLTTETQAEYRLGPIRIPHKVRERKKAKGILYVGYERGRLFGRELWLNMTDLLRHMVFFG
TTGSGKTETFYGFIVNFLLWCRGYCLSDGKADNKLAFATWSLARRFGREDDYYVLNLLTGSIDRFVNLVK
QESIPAQSNSVNLFSVAPPTFIIQLMESMLPQVGGDSAQWQDTAKAMMSALINALCYKRARGELLLSQRT
IQKNMSLPAMAALYVEAKKNGWHSEGYAALESYLENTPGFLLANAEYPETWEARAFEQHNYMSRQFLKTL
SLFNETYGHVFPEDSGDIRMDDIFHNDRILIVMIPSLELSRGEAATLGRLYVTLQRMTISKDLGYQLEGK
KEEVLLTHALNNQAPYGLIYDELGQYFTSGMDTLSAQMRSLEKMGVFSSQDHPSLARGANGEVDSLIANT
RVKYFESIEDRKTFEILRETVGQDYYSELSGQEAEHGTFSKTVREGDLYQIREKDRVNIRELRKQTEGQG
VISFQDALVRSAAFYIPDNEKFSSELPMRINRFIEVLPPSPATLYSIYPERKDDELAETNPDAMRFPPIK
LFGYDKAANSLLEKIEVFYELQCGAISPEEMSVCLFELFAEWLDGTETDDVLYHQEDDLFPMIEM
MQQESVDSKKVLRPDGSFMEWFLTPNVQFGLLAILTVLGLFLPFTMLLSILILPPLMVAFTDRKFRPPLR
MPKDCEMFDDTLTTETQAEYRLGPIRIPHKVRERKKAKGILYVGYERGRLFGRELWLNMTDLLRHMVFFG
TTGSGKTETFYGFIVNFLLWCRGYCLSDGKADNKLAFATWSLARRFGREDDYYVLNLLTGSIDRFVNLVK
QESIPAQSNSVNLFSVAPPTFIIQLMESMLPQVGGDSAQWQDTAKAMMSALINALCYKRARGELLLSQRT
IQKNMSLPAMAALYVEAKKNGWHSEGYAALESYLENTPGFLLANAEYPETWEARAFEQHNYMSRQFLKTL
SLFNETYGHVFPEDSGDIRMDDIFHNDRILIVMIPSLELSRGEAATLGRLYVTLQRMTISKDLGYQLEGK
KEEVLLTHALNNQAPYGLIYDELGQYFTSGMDTLSAQMRSLEKMGVFSSQDHPSLARGANGEVDSLIANT
RVKYFESIEDRKTFEILRETVGQDYYSELSGQEAEHGTFSKTVREGDLYQIREKDRVNIRELRKQTEGQG
VISFQDALVRSAAFYIPDNEKFSSELPMRINRFIEVLPPSPATLYSIYPERKDDELAETNPDAMRFPPIK
LFGYDKAANSLLEKIEVFYELQCGAISPEEMSVCLFELFAEWLDGTETDDVLYHQEDDLFPMIEM
Protein domains
No domain identified.
Protein structure
No available structure.
T4SS
T4SS were predicted by using oriTfinder2.
Region 1: 1197..20393
| Locus tag | Coordinates | Strand | Size (bp) | Protein ID | Product | Description |
|---|---|---|---|---|---|---|
| DLJ73_RS27385 (DLJ73_27385) | 700..1200 | + | 501 | WP_004206925 | DotD/TraH family lipoprotein | - |
| DLJ73_RS27390 (DLJ73_27390) | 1197..1976 | + | 780 | WP_004187315 | type IV secretory system conjugative DNA transfer family protein | traI |
| DLJ73_RS27395 (DLJ73_27395) | 1987..3150 | + | 1164 | WP_004206926 | plasmid transfer ATPase TraJ | virB11 |
| DLJ73_RS27400 (DLJ73_27400) | 3140..3400 | + | 261 | WP_004187310 | IcmT/TraK family protein | traK |
| DLJ73_RS28950 | 3425..6730 | + | 3306 | WP_219818010 | LPD7 domain-containing protein | - |
| DLJ73_RS27410 (DLJ73_27410) | 6696..7208 | + | 513 | WP_011091071 | hypothetical protein | traL |
| DLJ73_RS27415 (DLJ73_27415) | 7209..7421 | - | 213 | WP_305953721 | Hha/YmoA family nucleoid-associated regulatory protein | - |
| DLJ73_RS27420 (DLJ73_27420) | 7393..7803 | - | 411 | WP_004187465 | H-NS family nucleoid-associated regulatory protein | - |
| DLJ73_RS27425 (DLJ73_27425) | 7871..8584 | + | 714 | WP_223294958 | DotI/IcmL family type IV secretion protein | traM |
| DLJ73_RS27430 (DLJ73_27430) | 8593..9744 | + | 1152 | WP_015062843 | DotH/IcmK family type IV secretion protein | traN |
| DLJ73_RS27435 (DLJ73_27435) | 9756..11105 | + | 1350 | WP_004206932 | conjugal transfer protein TraO | traO |
| DLJ73_RS27440 (DLJ73_27440) | 11117..11821 | + | 705 | WP_004206933 | conjugal transfer protein TraP | traP |
| DLJ73_RS27445 (DLJ73_27445) | 11845..12375 | + | 531 | WP_004187478 | conjugal transfer protein TraQ | traQ |
| DLJ73_RS27450 (DLJ73_27450) | 12392..12781 | + | 390 | WP_011154472 | DUF6750 family protein | traR |
| DLJ73_RS27455 (DLJ73_27455) | 12827..13321 | + | 495 | WP_004187480 | hypothetical protein | - |
| DLJ73_RS27460 (DLJ73_27460) | 13318..16368 | + | 3051 | WP_004206934 | hypothetical protein | traU |
| DLJ73_RS27465 (DLJ73_27465) | 16365..17573 | + | 1209 | WP_004187486 | conjugal transfer protein TraW | traW |
| DLJ73_RS27470 (DLJ73_27470) | 17570..18220 | + | 651 | WP_004187488 | hypothetical protein | - |
| DLJ73_RS27475 (DLJ73_27475) | 18213..20393 | + | 2181 | WP_004187492 | DotA/TraY family protein | traY |
| DLJ73_RS27480 (DLJ73_27480) | 20396..21049 | + | 654 | WP_004206935 | hypothetical protein | - |
| DLJ73_RS27485 (DLJ73_27485) | 21123..21353 | + | 231 | WP_004187496 | IncL/M type plasmid replication protein RepC | - |
| DLJ73_RS27490 (DLJ73_27490) | 21650..22705 | + | 1056 | WP_015062846 | plasmid replication initiator RepA | - |
| DLJ73_RS27495 (DLJ73_27495) | 23877..24410 | - | 534 | Protein_23 | IS110-like element IS4321 family transposase | - |
Host bacterium
| ID | 18965 | GenBank | NZ_CP029599 |
| Plasmid name | pDA33145-77 | Incompatibility group | IncL/M |
| Plasmid size | 77110 bp | Coordinate of oriT [Strand] | 75405..75509 [+] |
| Host baterium | Klebsiella quasipneumoniae subsp. similipneumoniae strain ATCC 700603 |
Cargo genes
| Drug resistance gene | ant(2'')-Ia, aadA6, blaOXA-2, qacE, sul1, blaSHV-18 |
| Virulence gene | - |
| Metal resistance gene | merE, merD, merA, merC, merP, merT, merR |
| Degradation gene | - |
| Symbiosis gene | - |
| Anti-CRISPR | - |