Detailed information of oriT

oriT

The information of the oriT region


oriTDB ID   118528
Name   oriT_CNR130 in_silico
Organism   Klebsiella variicola isolate CNR130
Sequence Completeness      -
NCBI accession of oriT (coordinates [strand])   NZ_LT994838 (18904..19009 [+], 106 nt)
oriT length   106 nt
IRs (inverted repeats)      30..35, 41..46  (CCGCCG..CGGCGG)
Location of nic site      _
Conserved sequence flanking the
  nic site    		  _
Note   Predicted by oriTfinder 2.0

  oriT sequence  


Download         Length: 106 nt

>oriT_CNR130
AGTACGGGACAAGATGTGTTTTTGGAGTACCGCCGACACGCGGCGGCCGTCCAAAAACGTCTTGGTCAGGGCAAGCCCCGACACCCCCTAACGAGGTTAGCTATCT

Visualization of oriT structure

  oriT secondary structure

Predicted by RNAfold.

Download structure file


Relaxase

ID   11761 GenBank   WP_015058937
Name   traI_GWB07_RS00170_CNR130 insolico UniProt ID   A0A7G3L766
Length   659 a.a. PDB ID   
Note   Predicted by oriTfinder 2.0

  Relaxase protein sequence


Download         Length: 659 a.a.        Molecular weight: 75223.04 Da        Isoelectric Point: 9.9948

>WP_015058937.1 MULTISPECIES: TraI/MobA(P) family conjugative relaxase [Enterobacterales]
MVIPKIIEGRRDKKSSFGQLIKYMADKPSQELTDTVQPTPETVLAVKSDMFEGLNNYLIRKQKVISTPVD
VEPGVQRVVVGDVTCQYNTFSLDGAAQEMNSVSQQNTRCKDPVMHYVLSWPDYEKPNDDQVFDSVKFTLA
SMGMSDHQYVAAIHRDTDNLHVHVAVNRINPQTYKAASSSFTKDTLHQACRLLELKNGWSHSNGAYVVND
RQQIVRNPHSKKERGNWRSLDRINKMENKEGVETLYRYIVGDEQVGGSRQNLIHVSAGLREAKSWDDVHK
TFADIGLRVEKAQGKKGYVITHEHQNQKTAVKASLVFNKAQYTLKSMEERFGEYQPSHIEPAKVSVFKTA
YTPGAYRRDANKRLQRKIERAEERMLLKGRYRAYRNNLPIYSPDKDRIADEYRKIAQHTRLVKNNVRHSV
SDPHTRKLMYNLAEFKRLQAVANLRLSLREERNGFRAANPRLSYREWVEQEALKGDKAALSQMRGFAYSS
RKKEKYKQQLVEQIGFNRTFNAITSHDRDDVAVMASARHGVKPRLLKDGTVIFERDGKPVAADRGHIVLT
ESNGIDKEKTADLAIALTIAGKAKSVRVDGDGEFKELCCNRIVDAAVNHNLPVAQGITFTDAAQQAYAQN
EKHRLIREQNNSKNEMQFRSESDDKFNPK

  Protein domains


Predicted by InterproScan.

Relaxase/Mobilisation nuclease domain (Relaxase)

(87-334)

Large polyvalent protein-associated domain 7 (LPD7)

(530-630)


  Protein structure


Source ID Structure
AlphaFold DB A0A7G3L766


T4CP

ID   13776 GenBank   WP_004187443
Name   t4cp2_GWB07_RS00285_CNR130 insolico UniProt ID   _
Length   695 a.a. PDB ID   _
Note   Predicted by oriTfinder 2.0

  T4CP protein sequence


Download         Length: 695 a.a.        Molecular weight: 79519.87 Da        Isoelectric Point: 4.8309

>WP_004187443.1 MULTISPECIES: hypothetical protein [Enterobacterales]
MQQESVDSKKVIRPDGSFMEWFLTPNVQFGLLAILTVLGLFLPFTMLLSILILPPLMVAFTDRKFRPPLR
MPKDCEMFDDTLTTETQAEYRLGPIRIPHKVRERKKAKGILYVGYERGRLFGRELWLNMTDLLRHMVFFG
TTGSGKTETFYGFIVNFLLWCRGYCLSDGKADNKLAFATWSLARRFGREDDYYVLNLLTGSIDRFVNLVK
QESIPAQSNSVNLFSVAPPTFIIQLMESMLPQVGGDSAQWQDTAKAMMSALINALCYKRARGELLLSQRT
IQKNMSLPAMAALYVEAKKNGWHSEGYAALESYLENTPGFLLANAEYPETWEARAFEQHNYMSRQFLKTL
SLFNETYGHVFPEDSGDIRMDDIFHNDRILIVMIPSLELSRGEAATLGRLYVTLQRMTISKDLGYQLEGK
KEEVLLTHALNNQAPYGLIYDELGQYFTSGMDTLSAQMRSLEKMGVFSSQDHPSLARGANGEVDSLIANT
RVKYFESIEDRKTFEILRETVGQDYYSELSGQEAEHGTFSKTVREGDLYQIREKDRVNIRELRKQTEGQG
VISFQDALVRSAAFYIPDNEKFSSELPMRINRFIEVLPPSPATLYSIYPERKDDELAETNPDAMHFPPIK
LFGYDKAANSLLEKIEVFYELQCGAISPEEMSVCLFELFAEWLDGTETDDVLYHQEDDLFPMIEM

  Protein domains



No domain identified.


  Protein structure



No available structure.




T4SS

T4SS were predicted by using oriTfinder2.

Region 1: 21805..49053

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
GWB07_RS00150 (PCNR130_0035) 17001..17201 + 201 WP_228156189 hypothetical protein -
GWB07_RS00155 (PCNR130_0036) 17334..17870 + 537 WP_015058935 hypothetical protein -
GWB07_RS00160 (PCNR130_0039) 18371..18766 - 396 WP_019725163 hypothetical protein -
GWB07_RS00475 18765..19328 + 564 WP_227537722 plasmid mobilization protein MobA -
GWB07_RS00170 (PCNR130_0041) 19315..21294 + 1980 WP_015058937 TraI/MobA(P) family conjugative relaxase -
GWB07_RS00175 (PCNR130_0042) 21308..21808 + 501 WP_004187320 DotD/TraH family lipoprotein -
GWB07_RS00180 (PCNR130_0043) 21805..22584 + 780 WP_111961975 type IV secretory system conjugative DNA transfer family protein traI
GWB07_RS00185 (PCNR130_0044) 22595..23758 + 1164 WP_004187313 plasmid transfer ATPase TraJ virB11
GWB07_RS00190 (PCNR130_0045) 23748..24008 + 261 WP_004187310 IcmT/TraK family protein traK
GWB07_RS00480 (PCNR130_0046) 24033..27335 + 3303 WP_228156190 LPD7 domain-containing protein -
GWB07_RS00200 (PCNR130_0047) 27259..27813 + 555 WP_015243643 hypothetical protein traL
GWB07_RS00205 (PCNR130_0048) 27814..28011 - 198 WP_004187464 Hha/YmoA family nucleoid-associated regulatory protein -
GWB07_RS00210 (PCNR130_0049) 27998..28408 - 411 WP_004187465 H-NS family nucleoid-associated regulatory protein -
GWB07_RS00215 (PCNR130_0050) 28407..29189 + 783 WP_015058941 DotI/IcmL family type IV secretion protein traM
GWB07_RS00220 (PCNR130_0051) 29198..30349 + 1152 WP_011091074 DotH/IcmK family type IV secretion protein traN
GWB07_RS00225 (PCNR130_0052) 30361..31710 + 1350 WP_015058942 conjugal transfer protein TraO traO
GWB07_RS00230 (PCNR130_0053) 31722..32426 + 705 WP_015243644 conjugal transfer protein TraP traP
GWB07_RS00235 (PCNR130_0054) 32450..32980 + 531 WP_011091077 conjugal transfer protein TraQ traQ
GWB07_RS00240 (PCNR130_0055) 32997..33386 + 390 WP_011091078 DUF6750 family protein traR
GWB07_RS00245 (PCNR130_0056) 33432..33926 + 495 WP_015243645 hypothetical protein -
GWB07_RS00250 (PCNR130_0057) 33923..36973 + 3051 WP_031942490 plasmid transfer protein traU
GWB07_RS00255 (PCNR130_0058) 36970..38178 + 1209 WP_011091082 conjugal transfer protein TraW traW
GWB07_RS00260 (PCNR130_0059) 38175..38825 + 651 WP_011091083 hypothetical protein -
GWB07_RS00265 (PCNR130_0060) 38818..40998 + 2181 WP_004187492 DotA/TraY family protein traY
GWB07_RS00270 (PCNR130_0061) 41001..41654 + 654 WP_004187494 hypothetical protein -
GWB07_RS00275 (PCNR130_0062) 41711..41959 + 249 WP_015062836 IncL/M type plasmid replication protein RepC -
GWB07_RS00280 (PCNR130_0063) 42245..43312 + 1068 WP_052671755 plasmid replication initiator RepA -
GWB07_RS00490 44539..44634 + 96 WP_004206884 DinQ-like type I toxin DqlB -
GWB07_RS00285 (PCNR130_0066) 44685..46772 - 2088 WP_004187443 hypothetical protein -
GWB07_RS00290 (PCNR130_0067) 46785..47735 - 951 WP_111961976 DsbC family protein trbB
GWB07_RS00295 (PCNR130_0068) 47746..49053 - 1308 WP_015062796 hypothetical protein trbA
GWB07_RS00300 (PCNR130_0069) 49053..49448 - 396 WP_004187436 lytic transglycosylase domain-containing protein -
GWB07_RS00305 (PCNR130_0070) 49553..49948 - 396 WP_015062797 DUF1496 domain-containing protein -
GWB07_RS00310 (PCNR130_0071) 50016..50669 + 654 WP_039592068 CPBP family intramembrane glutamic endopeptidase -
GWB07_RS00315 (PCNR130_0072) 50821..51255 + 435 WP_015062799 LexA family transcriptional regulator -
GWB07_RS00320 (PCNR130_0073) 51243..52508 + 1266 WP_040224115 translesion error-prone DNA polymerase V subunit UmuC -
GWB07_RS00325 (PCNR130_0074) 52531..53379 - 849 WP_060453257 3'-5' exonuclease -
GWB07_RS00330 (PCNR130_0075) 53382..53702 - 321 WP_021561451 hypothetical protein -

Host bacterium

ID   18961 GenBank   NZ_LT994838
Plasmid name   CNR130 Incompatibility group   IncL/M
Plasmid size   69498 bp Coordinate of oriT [Strand]   18904..19009 [+]
Host baterium   Klebsiella variicola isolate CNR130

Cargo genes

Drug resistance gene   sul1, blaDHA-1, qnrB4
Virulence gene   -
Metal resistance gene   -
Degradation gene   -
Symbiosis gene   -
Anti-CRISPR   -