Detailed information of oriT
oriT
The information of the oriT region
| oriTDB ID | 118432 |
| Name | oriT_SC1762-D|p3  |
| Organism | Enterococcus faecium strain SC1762-D |
| Sequence Completeness | - |
| NCBI accession of oriT (coordinates [strand]) | NZ_CP085909 (30935..31126 [+], 192 nt) |
| oriT length | 192 nt |
| IRs (inverted repeats) | 106..112, 122..128 (ATTTTTT..AAAAAAT) 107..113, 120..126 (TTTTTTG..CAAAAAA) 93..98, 105..110 (AAAATA..TATTTT) 65..71, 75..81 (AGTTGGC..GCCAACT) 41..49, 52..60 (CACCTTCCT..AGGAAGGTG) |
| Location of nic site | _ |
| Conserved sequence flanking the nic site |
_ |
| Note | Predicted by oriTfinder 2.0 |
oriT sequence
Download Length: 192 nt
>oriT_SC1762-D|p3
CCAAGGAATTAATACAAAGAGCATAAGGGAAAACTGATAGCACCTTCCTAAAGGAAGGTGGCTAAGTTGGCTGTGCCAACTGGTTTTCTTTCAAAATAGCTTCATATTTTTTGCTATCACAAAAAAATCCATTTTCGACCTATTTTCGGTCATAATATAGTACCTACTTTTGGTCATAGTTTCGTCCGTAGT
CCAAGGAATTAATACAAAGAGCATAAGGGAAAACTGATAGCACCTTCCTAAAGGAAGGTGGCTAAGTTGGCTGTGCCAACTGGTTTTCTTTCAAAATAGCTTCATATTTTTTGCTATCACAAAAAAATCCATTTTCGACCTATTTTCGGTCATAATATAGTACCTACTTTTGGTCATAGTTTCGTCCGTAGT
Visualization of oriT structure
oriT secondary structure
Predicted by RNAfold.
Download structure file
Relaxase
| ID | 11710 | GenBank | WP_002326870 |
| Name | mobP2_LLE91_RS15940_SC1762-D|p3 |
UniProt ID | B5U8V6 |
| Length | 506 a.a. | PDB ID | |
| Note | Predicted by oriTfinder 2.0 | ||
Relaxase protein sequence
Download Length: 506 a.a. Molecular weight: 60597.01 Da Isoelectric Point: 9.3485
>WP_002326870.1 MULTISPECIES: MobP2 family relaxase [Enterococcus]
MTEHIFNRKSASIILKAKFETGKSKKNNHMVELQKFVDYISRQEAIRQDKLDHDYSEDELNELARIEKAL
EKIEQETDQPVIKDLREMDKYIDYMTRKKAILENVDNEIVNGAFSNTKRYITKKDISKIKESVIEAKNNG
SVMFQDVISFDNDFLVREGYYNPETNELNENVLYEATKSMMGKMQEKEELVDPFWFATIHRNTEHIHIHV
TAMERKNTREIMEYDGVLQARGKRKQSTLDDMIFKFGSKILDRTNEFEKISKLRKEVPLELKQSVKDSLI
QLYVENNSRYDKELVKYLKELKKEIPSTTRGYNELPEETKEKIDEVTNYMTKDNPKRKEYDRMTKEIDEL
YQTTYGKRYNPQEYYLNRKKDFNARMGNAVVGQIKLLKQSEEKNLKSNELKNLLDGKKLNVKFDREKPEF
KTKYAKQSYDDFQKRLEERQLAREKNEVHFKKISEKFKDKKLIRKIDRVINDDLNMYRAKNDYEVAQRMI
EQEKMRQAREFEIGMN
MTEHIFNRKSASIILKAKFETGKSKKNNHMVELQKFVDYISRQEAIRQDKLDHDYSEDELNELARIEKAL
EKIEQETDQPVIKDLREMDKYIDYMTRKKAILENVDNEIVNGAFSNTKRYITKKDISKIKESVIEAKNNG
SVMFQDVISFDNDFLVREGYYNPETNELNENVLYEATKSMMGKMQEKEELVDPFWFATIHRNTEHIHIHV
TAMERKNTREIMEYDGVLQARGKRKQSTLDDMIFKFGSKILDRTNEFEKISKLRKEVPLELKQSVKDSLI
QLYVENNSRYDKELVKYLKELKKEIPSTTRGYNELPEETKEKIDEVTNYMTKDNPKRKEYDRMTKEIDEL
YQTTYGKRYNPQEYYLNRKKDFNARMGNAVVGQIKLLKQSEEKNLKSNELKNLLDGKKLNVKFDREKPEF
KTKYAKQSYDDFQKRLEERQLAREKNEVHFKKISEKFKDKKLIRKIDRVINDDLNMYRAKNDYEVAQRMI
EQEKMRQAREFEIGMN
Protein domains
Predicted by InterproScan.
Protein structure
| Source | ID | Structure |
|---|---|---|
| AlphaFold DB | B5U8V6 |
T4CP
| ID | 13711 | GenBank | WP_123061283 |
| Name | t4cp2_LLE91_RS16010_SC1762-D|p3 |
UniProt ID | _ |
| Length | 952 a.a. | PDB ID | _ |
| Note | Predicted by oriTfinder 2.0 | ||
T4CP protein sequence
Download Length: 952 a.a. Molecular weight: 108310.19 Da Isoelectric Point: 7.4752
>WP_123061283.1 VirD4-like conjugal transfer protein, CD1115 family [Enterococcus faecium]
MKLVQNNEVKEIKNGFVKNSNKILGKLKKVGSFKKNKYHTGKIEGEDLPKSLQDKLNVPLVFIGVFLGSI
VFLAITYLLNFLKALLVAIGQSESGLLGVEFDPKFSLAYLKPLGDTKQYFMCAVVAVIGVAYLIYGKLNH
ESEKNLVYGQKGDSRLSTIKEIKRDYHEIPEKTEVYDGIGGVPISHYQDKYYIDRDTVNTVVLGASRSGK
GETFVVPLLDNLSRAKIQSNMVVNDPKGELFSASKETLEKRGYRVLVLNIDDPLESMSFNPLQLVIDSWA
NKDYHEASKRANTLTSMLFASGMGTDNEFFYKSAKSAVNAIILTIVEHCFNNDCIEKITMYNVAQMLNEL
GSLFYTDPKTRKEKNALDEYFNTLPQGNQAKIQYGSTSFAGDKAKGSILSTASQGIEMFTSDLFGKLTSK
MSIDLKEIGFPKSIQFKVNTNLVGKRISISFLRKENDVIRLIKKYRVKVKALGLCVLNFNEYLQDGDMLD
IRYEEEKSRAWYRIEFPKKQANSHDTSLVTKKFKSGKNYSDLEISTLRLKYTDQPTAVFMVIPDYDPSNH
VLASIFISQLYTELASNCKQTPDKKCFRRVHFLLDEFGNMPAIDNMDGIMTVCLGRNMLFDLVIQSYSQL
ETRYDKAFKTIKENCQNHILIMSNDEETIEEISKKCGHKTTINRSASSKHLDTDSSVTSSAEQERVITVE
RASQLIEGEQIILRNLHRQDTKRNKIRPFPIFNTKETNMPYRYQFLAEDFDTQRDINEIDIACEHINLSL
QDNQIPYARFIKDLKTRLEYSIINNIPISEEDYQDYLNLSGSQQTINEEELLKLVNTEVKSQDMEGQVLS
EQEMKINIYLKESQKEAIELVKVINGRLADENSADMVIKTINSQILDTPFIQSLINDSIAIHNVQESKDQ
IMILEQQLANAYELAVFKTKNSILNNKIIKLKNAMTKLAKAI
MKLVQNNEVKEIKNGFVKNSNKILGKLKKVGSFKKNKYHTGKIEGEDLPKSLQDKLNVPLVFIGVFLGSI
VFLAITYLLNFLKALLVAIGQSESGLLGVEFDPKFSLAYLKPLGDTKQYFMCAVVAVIGVAYLIYGKLNH
ESEKNLVYGQKGDSRLSTIKEIKRDYHEIPEKTEVYDGIGGVPISHYQDKYYIDRDTVNTVVLGASRSGK
GETFVVPLLDNLSRAKIQSNMVVNDPKGELFSASKETLEKRGYRVLVLNIDDPLESMSFNPLQLVIDSWA
NKDYHEASKRANTLTSMLFASGMGTDNEFFYKSAKSAVNAIILTIVEHCFNNDCIEKITMYNVAQMLNEL
GSLFYTDPKTRKEKNALDEYFNTLPQGNQAKIQYGSTSFAGDKAKGSILSTASQGIEMFTSDLFGKLTSK
MSIDLKEIGFPKSIQFKVNTNLVGKRISISFLRKENDVIRLIKKYRVKVKALGLCVLNFNEYLQDGDMLD
IRYEEEKSRAWYRIEFPKKQANSHDTSLVTKKFKSGKNYSDLEISTLRLKYTDQPTAVFMVIPDYDPSNH
VLASIFISQLYTELASNCKQTPDKKCFRRVHFLLDEFGNMPAIDNMDGIMTVCLGRNMLFDLVIQSYSQL
ETRYDKAFKTIKENCQNHILIMSNDEETIEEISKKCGHKTTINRSASSKHLDTDSSVTSSAEQERVITVE
RASQLIEGEQIILRNLHRQDTKRNKIRPFPIFNTKETNMPYRYQFLAEDFDTQRDINEIDIACEHINLSL
QDNQIPYARFIKDLKTRLEYSIINNIPISEEDYQDYLNLSGSQQTINEEELLKLVNTEVKSQDMEGQVLS
EQEMKINIYLKESQKEAIELVKVINGRLADENSADMVIKTINSQILDTPFIQSLINDSIAIHNVQESKDQ
IMILEQQLANAYELAVFKTKNSILNNKIIKLKNAMTKLAKAI
Protein domains
Predicted by InterproScan.
Protein structure
No available structure.
T4SS
T4SS were predicted by using oriTfinder2.
Region 1: 33038..51877
| Locus tag | Coordinates | Strand | Size (bp) | Protein ID | Product | Description |
|---|---|---|---|---|---|---|
| LLE91_RS15935 (LLE91_15935) | 28133..28462 | - | 330 | WP_002311869 | hypothetical protein | - |
| LLE91_RS15940 (LLE91_15940) | 28484..30004 | - | 1521 | WP_002326870 | MobP2 family relaxase | - |
| LLE91_RS15945 (LLE91_15945) | 30086..30553 | - | 468 | WP_002311867 | hypothetical protein | - |
| LLE91_RS15950 (LLE91_15950) | 30550..30846 | - | 297 | WP_002311865 | hypothetical protein | - |
| LLE91_RS15955 (LLE91_15955) | 31197..31427 | - | 231 | WP_002311864 | hypothetical protein | - |
| LLE91_RS15960 (LLE91_15960) | 31463..32359 | - | 897 | WP_202979420 | hypothetical protein | - |
| LLE91_RS15965 (LLE91_15965) | 32373..33023 | - | 651 | WP_002311862 | cell division protein FtsL | - |
| LLE91_RS15970 (LLE91_15970) | 33038..34174 | - | 1137 | WP_002311861 | bifunctional lytic transglycosylase/C40 family peptidase | orf14 |
| LLE91_RS15975 (LLE91_15975) | 34196..34516 | - | 321 | WP_010729568 | hypothetical protein | virb4 |
| LLE91_RS15980 (LLE91_15980) | 34704..35744 | - | 1041 | WP_202979419 | HNH endonuclease | - |
| LLE91_RS15985 (LLE91_15985) | 36432..38057 | - | 1626 | WP_010729563 | VirB4 family type IV secretion system protein | virb4 |
| LLE91_RS15990 (LLE91_15990) | 38073..38540 | - | 468 | WP_002311859 | hypothetical protein | - |
| LLE91_RS15995 (LLE91_15995) | 38557..39174 | - | 618 | WP_002311858 | hypothetical protein | virb4 |
| LLE91_RS16000 (LLE91_16000) | 39171..39482 | - | 312 | WP_002317413 | DUF5592 family protein | - |
| LLE91_RS16005 (LLE91_16005) | 39530..42214 | - | 2685 | WP_227889218 | pLS20_p028 family conjugation system transmembrane protein | - |
| LLE91_RS16010 (LLE91_16010) | 42232..45090 | - | 2859 | WP_123061283 | VirD4-like conjugal transfer protein, CD1115 family | - |
| LLE91_RS16015 (LLE91_16015) | 45103..45819 | - | 717 | WP_202979415 | hypothetical protein | - |
| LLE91_RS16020 (LLE91_16020) | 45832..47658 | - | 1827 | WP_002311853 | hypothetical protein | - |
| LLE91_RS16025 (LLE91_16025) | 47766..48041 | - | 276 | WP_002311852 | TrbC/VirB2 family protein | virB2 |
| LLE91_RS16030 (LLE91_16030) | 48060..48281 | - | 222 | WP_002326867 | hypothetical protein | - |
| LLE91_RS16035 (LLE91_16035) | 48293..48439 | - | 147 | WP_002326866 | hypothetical protein | - |
| LLE91_RS16040 (LLE91_16040) | 48453..48815 | - | 363 | WP_002311851 | DUF4320 family protein | - |
| LLE91_RS16045 (LLE91_16045) | 48833..49630 | - | 798 | WP_002311850 | hypothetical protein | - |
| LLE91_RS16050 (LLE91_16050) | 49630..50445 | - | 816 | WP_002313315 | hypothetical protein | - |
| LLE91_RS16055 (LLE91_16055) | 50462..51877 | - | 1416 | WP_002311848 | CpaF/VirB11 family protein | virB11 |
| LLE91_RS16060 (LLE91_16060) | 51889..52311 | - | 423 | WP_002311847 | hypothetical protein | - |
| LLE91_RS16065 (LLE91_16065) | 52379..52531 | - | 153 | WP_222051374 | hypothetical protein | - |
| LLE91_RS16070 (LLE91_16070) | 52545..52907 | - | 363 | WP_002311845 | hypothetical protein | - |
| LLE91_RS16075 (LLE91_16075) | 52967..56596 | - | 3630 | WP_002311844 | collagen binding domain-containing protein | - |
Host bacterium
| ID | 18865 | GenBank | NZ_CP085909 |
| Plasmid name | SC1762-D|p3 | Incompatibility group | - |
| Plasmid size | 63475 bp | Coordinate of oriT [Strand] | 30935..31126 [+] |
| Host baterium | Enterococcus faecium strain SC1762-D |
Cargo genes
| Drug resistance gene | - |
| Virulence gene | - |
| Metal resistance gene | - |
| Degradation gene | - |
| Symbiosis gene | - |
| Anti-CRISPR | AcrIIA1 |