Detailed information of oriT
oriT
The information of the oriT region
| oriTDB ID | 118428 |
| Name | oriT_SC1762|p3  |
| Organism | Enterococcus faecium strain SC1762 |
| Sequence Completeness | - |
| NCBI accession of oriT (coordinates [strand]) | NZ_CP085897 (40893..41084 [-], 192 nt) |
| oriT length | 192 nt |
| IRs (inverted repeats) | 106..112, 122..128 (ATTTTTT..AAAAAAT) 107..113, 120..126 (TTTTTTG..CAAAAAA) 93..98, 105..110 (AAAATA..TATTTT) 65..71, 75..81 (AGTTGGC..GCCAACT) 41..49, 52..60 (CACCTTCCT..AGGAAGGTG) |
| Location of nic site | _ |
| Conserved sequence flanking the nic site |
_ |
| Note | Predicted by oriTfinder 2.0 |
oriT sequence
Download Length: 192 nt
>oriT_SC1762|p3
CCAAGGAATTAATACAAAGAGCATAAGGGAAAACTGATAGCACCTTCCTAAAGGAAGGTGGCTAAGTTGGCTGTGCCAACTGGTTTTCTTTCAAAATAGCTTCATATTTTTTGCTATCACAAAAAAATCCATTTTCGACCTATTTTCGGTCATAATATAGTACCTACTTTTGGTCATAGTTTCGTCCGTAGT
CCAAGGAATTAATACAAAGAGCATAAGGGAAAACTGATAGCACCTTCCTAAAGGAAGGTGGCTAAGTTGGCTGTGCCAACTGGTTTTCTTTCAAAATAGCTTCATATTTTTTGCTATCACAAAAAAATCCATTTTCGACCTATTTTCGGTCATAATATAGTACCTACTTTTGGTCATAGTTTCGTCCGTAGT
Visualization of oriT structure
oriT secondary structure
Predicted by RNAfold.
Download structure file
Relaxase
| ID | 11708 | GenBank | WP_002326870 |
| Name | mobP2_LK894_RS15975_SC1762|p3 |
UniProt ID | B5U8V6 |
| Length | 506 a.a. | PDB ID | |
| Note | Predicted by oriTfinder 2.0 | ||
Relaxase protein sequence
Download Length: 506 a.a. Molecular weight: 60597.01 Da Isoelectric Point: 9.3485
>WP_002326870.1 MULTISPECIES: MobP2 family relaxase [Enterococcus]
MTEHIFNRKSASIILKAKFETGKSKKNNHMVELQKFVDYISRQEAIRQDKLDHDYSEDELNELARIEKAL
EKIEQETDQPVIKDLREMDKYIDYMTRKKAILENVDNEIVNGAFSNTKRYITKKDISKIKESVIEAKNNG
SVMFQDVISFDNDFLVREGYYNPETNELNENVLYEATKSMMGKMQEKEELVDPFWFATIHRNTEHIHIHV
TAMERKNTREIMEYDGVLQARGKRKQSTLDDMIFKFGSKILDRTNEFEKISKLRKEVPLELKQSVKDSLI
QLYVENNSRYDKELVKYLKELKKEIPSTTRGYNELPEETKEKIDEVTNYMTKDNPKRKEYDRMTKEIDEL
YQTTYGKRYNPQEYYLNRKKDFNARMGNAVVGQIKLLKQSEEKNLKSNELKNLLDGKKLNVKFDREKPEF
KTKYAKQSYDDFQKRLEERQLAREKNEVHFKKISEKFKDKKLIRKIDRVINDDLNMYRAKNDYEVAQRMI
EQEKMRQAREFEIGMN
MTEHIFNRKSASIILKAKFETGKSKKNNHMVELQKFVDYISRQEAIRQDKLDHDYSEDELNELARIEKAL
EKIEQETDQPVIKDLREMDKYIDYMTRKKAILENVDNEIVNGAFSNTKRYITKKDISKIKESVIEAKNNG
SVMFQDVISFDNDFLVREGYYNPETNELNENVLYEATKSMMGKMQEKEELVDPFWFATIHRNTEHIHIHV
TAMERKNTREIMEYDGVLQARGKRKQSTLDDMIFKFGSKILDRTNEFEKISKLRKEVPLELKQSVKDSLI
QLYVENNSRYDKELVKYLKELKKEIPSTTRGYNELPEETKEKIDEVTNYMTKDNPKRKEYDRMTKEIDEL
YQTTYGKRYNPQEYYLNRKKDFNARMGNAVVGQIKLLKQSEEKNLKSNELKNLLDGKKLNVKFDREKPEF
KTKYAKQSYDDFQKRLEERQLAREKNEVHFKKISEKFKDKKLIRKIDRVINDDLNMYRAKNDYEVAQRMI
EQEKMRQAREFEIGMN
Protein domains
Predicted by InterproScan.
Protein structure
| Source | ID | Structure |
|---|---|---|
| AlphaFold DB | B5U8V6 |
T4CP
| ID | 13707 | GenBank | WP_123061283 |
| Name | t4cp2_LK894_RS15905_SC1762|p3 |
UniProt ID | _ |
| Length | 952 a.a. | PDB ID | _ |
| Note | Predicted by oriTfinder 2.0 | ||
T4CP protein sequence
Download Length: 952 a.a. Molecular weight: 108310.19 Da Isoelectric Point: 7.4752
>WP_123061283.1 VirD4-like conjugal transfer protein, CD1115 family [Enterococcus faecium]
MKLVQNNEVKEIKNGFVKNSNKILGKLKKVGSFKKNKYHTGKIEGEDLPKSLQDKLNVPLVFIGVFLGSI
VFLAITYLLNFLKALLVAIGQSESGLLGVEFDPKFSLAYLKPLGDTKQYFMCAVVAVIGVAYLIYGKLNH
ESEKNLVYGQKGDSRLSTIKEIKRDYHEIPEKTEVYDGIGGVPISHYQDKYYIDRDTVNTVVLGASRSGK
GETFVVPLLDNLSRAKIQSNMVVNDPKGELFSASKETLEKRGYRVLVLNIDDPLESMSFNPLQLVIDSWA
NKDYHEASKRANTLTSMLFASGMGTDNEFFYKSAKSAVNAIILTIVEHCFNNDCIEKITMYNVAQMLNEL
GSLFYTDPKTRKEKNALDEYFNTLPQGNQAKIQYGSTSFAGDKAKGSILSTASQGIEMFTSDLFGKLTSK
MSIDLKEIGFPKSIQFKVNTNLVGKRISISFLRKENDVIRLIKKYRVKVKALGLCVLNFNEYLQDGDMLD
IRYEEEKSRAWYRIEFPKKQANSHDTSLVTKKFKSGKNYSDLEISTLRLKYTDQPTAVFMVIPDYDPSNH
VLASIFISQLYTELASNCKQTPDKKCFRRVHFLLDEFGNMPAIDNMDGIMTVCLGRNMLFDLVIQSYSQL
ETRYDKAFKTIKENCQNHILIMSNDEETIEEISKKCGHKTTINRSASSKHLDTDSSVTSSAEQERVITVE
RASQLIEGEQIILRNLHRQDTKRNKIRPFPIFNTKETNMPYRYQFLAEDFDTQRDINEIDIACEHINLSL
QDNQIPYARFIKDLKTRLEYSIINNIPISEEDYQDYLNLSGSQQTINEEELLKLVNTEVKSQDMEGQVLS
EQEMKINIYLKESQKEAIELVKVINGRLADENSADMVIKTINSQILDTPFIQSLINDSIAIHNVQESKDQ
IMILEQQLANAYELAVFKTKNSILNNKIIKLKNAMTKLAKAI
MKLVQNNEVKEIKNGFVKNSNKILGKLKKVGSFKKNKYHTGKIEGEDLPKSLQDKLNVPLVFIGVFLGSI
VFLAITYLLNFLKALLVAIGQSESGLLGVEFDPKFSLAYLKPLGDTKQYFMCAVVAVIGVAYLIYGKLNH
ESEKNLVYGQKGDSRLSTIKEIKRDYHEIPEKTEVYDGIGGVPISHYQDKYYIDRDTVNTVVLGASRSGK
GETFVVPLLDNLSRAKIQSNMVVNDPKGELFSASKETLEKRGYRVLVLNIDDPLESMSFNPLQLVIDSWA
NKDYHEASKRANTLTSMLFASGMGTDNEFFYKSAKSAVNAIILTIVEHCFNNDCIEKITMYNVAQMLNEL
GSLFYTDPKTRKEKNALDEYFNTLPQGNQAKIQYGSTSFAGDKAKGSILSTASQGIEMFTSDLFGKLTSK
MSIDLKEIGFPKSIQFKVNTNLVGKRISISFLRKENDVIRLIKKYRVKVKALGLCVLNFNEYLQDGDMLD
IRYEEEKSRAWYRIEFPKKQANSHDTSLVTKKFKSGKNYSDLEISTLRLKYTDQPTAVFMVIPDYDPSNH
VLASIFISQLYTELASNCKQTPDKKCFRRVHFLLDEFGNMPAIDNMDGIMTVCLGRNMLFDLVIQSYSQL
ETRYDKAFKTIKENCQNHILIMSNDEETIEEISKKCGHKTTINRSASSKHLDTDSSVTSSAEQERVITVE
RASQLIEGEQIILRNLHRQDTKRNKIRPFPIFNTKETNMPYRYQFLAEDFDTQRDINEIDIACEHINLSL
QDNQIPYARFIKDLKTRLEYSIINNIPISEEDYQDYLNLSGSQQTINEEELLKLVNTEVKSQDMEGQVLS
EQEMKINIYLKESQKEAIELVKVINGRLADENSADMVIKTINSQILDTPFIQSLINDSIAIHNVQESKDQ
IMILEQQLANAYELAVFKTKNSILNNKIIKLKNAMTKLAKAI
Protein domains
Predicted by InterproScan.
Protein structure
No available structure.
T4SS
T4SS were predicted by using oriTfinder2.
Region 1: 20142..38981
| Locus tag | Coordinates | Strand | Size (bp) | Protein ID | Product | Description |
|---|---|---|---|---|---|---|
| LK894_RS15840 (LK894_15855) | 15423..19052 | + | 3630 | WP_002311844 | collagen binding domain-containing protein | - |
| LK894_RS15845 (LK894_15860) | 19112..19474 | + | 363 | WP_002311845 | hypothetical protein | - |
| LK894_RS15850 (LK894_15865) | 19488..19640 | + | 153 | WP_222051374 | hypothetical protein | - |
| LK894_RS15855 (LK894_15870) | 19708..20130 | + | 423 | WP_002311847 | hypothetical protein | - |
| LK894_RS15860 (LK894_15875) | 20142..21557 | + | 1416 | WP_002311848 | CpaF/VirB11 family protein | virB11 |
| LK894_RS15865 (LK894_15880) | 21574..22389 | + | 816 | WP_002313315 | hypothetical protein | - |
| LK894_RS15870 (LK894_15885) | 22389..23186 | + | 798 | WP_002311850 | hypothetical protein | - |
| LK894_RS15875 (LK894_15890) | 23204..23566 | + | 363 | WP_002311851 | DUF4320 family protein | - |
| LK894_RS15880 (LK894_15895) | 23580..23726 | + | 147 | WP_002326866 | hypothetical protein | - |
| LK894_RS15885 (LK894_15900) | 23738..23959 | + | 222 | WP_002326867 | hypothetical protein | - |
| LK894_RS15890 (LK894_15905) | 23978..24253 | + | 276 | WP_002311852 | TrbC/VirB2 family protein | virB2 |
| LK894_RS15895 (LK894_15910) | 24361..26187 | + | 1827 | WP_002311853 | hypothetical protein | - |
| LK894_RS15900 (LK894_15915) | 26200..26916 | + | 717 | WP_202979415 | hypothetical protein | - |
| LK894_RS15905 (LK894_15920) | 26929..29787 | + | 2859 | WP_123061283 | VirD4-like conjugal transfer protein, CD1115 family | - |
| LK894_RS15910 (LK894_15925) | 29805..32489 | + | 2685 | WP_227889218 | pLS20_p028 family conjugation system transmembrane protein | - |
| LK894_RS15915 (LK894_15930) | 32537..32848 | + | 312 | WP_002317413 | DUF5592 family protein | - |
| LK894_RS15920 (LK894_15935) | 32845..33462 | + | 618 | WP_002311858 | hypothetical protein | virb4 |
| LK894_RS15925 (LK894_15940) | 33479..33946 | + | 468 | WP_002311859 | hypothetical protein | - |
| LK894_RS15930 (LK894_15945) | 33962..35587 | + | 1626 | WP_010729563 | VirB4 family type IV secretion system protein | virb4 |
| LK894_RS15935 (LK894_15950) | 36275..37315 | + | 1041 | WP_202979419 | HNH endonuclease | - |
| LK894_RS15940 (LK894_15955) | 37503..37823 | + | 321 | WP_010729568 | hypothetical protein | virb4 |
| LK894_RS15945 (LK894_15960) | 37845..38981 | + | 1137 | WP_002311861 | bifunctional lytic transglycosylase/C40 family peptidase | orf14 |
| LK894_RS15950 (LK894_15965) | 38996..39646 | + | 651 | WP_002311862 | cell division protein FtsL | - |
| LK894_RS15955 (LK894_15970) | 39660..40556 | + | 897 | WP_202979420 | hypothetical protein | - |
| LK894_RS15960 (LK894_15975) | 40592..40822 | + | 231 | WP_002311864 | hypothetical protein | - |
| LK894_RS15965 (LK894_15980) | 41173..41469 | + | 297 | WP_002311865 | hypothetical protein | - |
| LK894_RS15970 (LK894_15985) | 41466..41933 | + | 468 | WP_002311867 | hypothetical protein | - |
| LK894_RS15975 (LK894_15990) | 42015..43535 | + | 1521 | WP_002326870 | MobP2 family relaxase | - |
| LK894_RS15980 (LK894_15995) | 43557..43886 | + | 330 | WP_002311869 | hypothetical protein | - |
Host bacterium
| ID | 18861 | GenBank | NZ_CP085897 |
| Plasmid name | SC1762|p3 | Incompatibility group | - |
| Plasmid size | 63475 bp | Coordinate of oriT [Strand] | 40893..41084 [-] |
| Host baterium | Enterococcus faecium strain SC1762 |
Cargo genes
| Drug resistance gene | - |
| Virulence gene | - |
| Metal resistance gene | - |
| Degradation gene | - |
| Symbiosis gene | - |
| Anti-CRISPR | AcrIIA1 |