Detailed information of oriT

oriT

The information of the oriT region

• oriTDB ID: 118411
• Name: oriT_AKSZ-142|unnamed1
• Organism: Enterococcus faecium strain AKSZ-142
• Sequence Completeness: -
• NCBI accession of oriT (coordinates [strand]): NZ_CP110361 (2219..2422 [-], 204 nt)
• oriT length: 204 nt
• IRs (inverted repeats): 125..130, 143..148 (AAATCC..GGATTT) 66..72, 83..89 (ACCCCCC..GGGGGGT)
• Location of nic site: 135..136
• Conserved sequence flanking the nic site: TTTGGTTACA
• Note: Predicted by oriTfinder 2.0

oriT sequence

• Length: 204 nt

>oriT_AKSZ-142|unnamed1
AAGCGGAAGTCGCAGGTGTGGACTGATCTTGCTGGCTGGTGTGGCAATAGCCACGCCAGCACTTAACCCCCCGTATCTAACAGGGGGGTACAAATCGACAGGAAACAGTCAAAAAAACATTAGAAAATCCTTTGGTTACAAGGGATTTACAAAATTTCAGCGTATGTCAAATGGGCTTTAAAAGTTGACATACGCCTTTTTGAT

oriT secondary structure

Predicted by RNAfold.

Relaxase

mobT_MZO31_RS14425_AKSZ-142|unnamed1

• ID: 11695
• GenBank: WP_000398284
• Name: mobT_MZO31_RS14425_AKSZ-142|unnamed1
• UniProt ID: A0A3P3PY43
• Length: 401 a.a.
• Note: Predicted by oriTfinder 2.0

Relaxase protein sequence

• Length: 401 a.a.
• Molecular weight: 47398.10 Da
• Isoelectric Point: 6.5091

>WP_000398284.1 MULTISPECIES: MobT family relaxase [Bacteria]
MEGFLLNEQTWLQHLKEKRLAYGLSQNRLAVATGITRQYLSDIETGKVKPSEDLQQSLWEALERFNPDAP
LEMLFDYVRIRFPTTDVQQVVENILQLKLSYFLHEDYGFYSYSEHYALGDIFVLCSHELDKGVLVELKGR
GCRQFESYLLAQQRSWYEFFMDVLVAGGVMKRLDLAINDKTGILNIPVLTEKCQQEECISVFRSFKSYRS
GELVRKEEKECMGNTLYIGSLQSEVYFCIYEKDYEQYKKNDIPIEDAEVKNRFEIRLKNERAYYAVRDLL
VYDNPEHTAFKIINRYIRFVDKDDSKPRSDWKLNEEWAWFIGNNRERLKLTTKPEPYSFQRTLNWLSHQV
APTLKVAIKLDEINQTQVVKDILDHAKLTDRHKQILKQQSVKEQDVITTKK

Protein domains

Predicted by InterproScan.

• Helix-turn-helix (HTH_3) (15-53)

• Replication initiation factor (Rep_trans) (169-373)

• Rolling Circle replication initiation protein N-terminal domain (Rol_Rep_N) (73-161)

Protein structure

• AlphaFold DB: A0A3P3PY43

T4CP

tcpA_MZO31_RS14435_AKSZ-142|unnamed1

• ID: 13694
• GenBank: WP_000813488
• Name: tcpA_MZO31_RS14435_AKSZ-142|unnamed1
• UniProt ID: _
• Length: 461 a.a.
• PDB ID: _
• Note: Predicted by oriTfinder 2.0

T4CP protein sequence

• Length: 461 a.a.
• Molecular weight: 53370.27 Da
• Isoelectric Point: 9.0687

>WP_000813488.1 MULTISPECIES: FtsK/SpoIIIE domain-containing protein [Bacteria]
MKQRGKRIRPSGKDLVFHFTIASLLPVFLLVVGLFHVKTIQQINWQDFNLSQADKIDIPYLIISFSVAIL
ICLLVAFVFKRVRYDTVKQLYHRQKLAKMILENKWYESEQVKTEGFFKDSAGRTKEKITYFPKMYYRLKN
GLIQIRVEITLGKYQDQLLHLEKKLESGLYCELTDKELKDSYVEYTLLYDTIASRISIDEVEAKDGKLRL
MKNVWWEYDKLPHMLIAGGTGGGKTYFILTLIEALLHTDSKLYILDPKNADLADLGSVMANVYYRKEDLL
SCIETFYEEMMKRSEEMKQMKNYKTGKNYAYLGLPAHFLIFDEYVAFMEMLGTKENTAVMNKLKQIVMLG
RQAGFFLILACQRPDAKYLGDGIRDQFNFRVALGRMSEMGYGMMFGSDVQKDFFLKRIKGRGYVDVGTSV
ISEFYTPLVPKGYDFLEEIKKLSNSRQSTQATCEAEVAGVD

Protein domains

Predicted by InterproScan.

• FtsK/SpoIIIE family (FtsK_SpoIIIE) (217-301)

Protein structure

No available structure.

Host bacterium

• ID: 18844
• GenBank: NZ_CP110361
• Plasmid name: AKSZ-142|unnamed1
• Incompatibility group: -
• Plasmid size: 54197 bp
• Coordinate of oriT [Strand]: 2219..2422 [-]
• Host baterium: Enterococcus faecium strain AKSZ-142

Cargo genes

• Drug resistance gene: erm(B), poxtA, fexB, tet(L), tet(M)
• Virulence gene: -
• Metal resistance gene: -
• Degradation gene: -
• Symbiosis gene: -
• Anti-CRISPR: -