Detailed information of oriT

oriT

The information of the oriT region


oriTDB ID   118337
Name   oriT_pIMPIncN3_57kb in_silico
Organism   Enterobacter kobei strain EB_P8_L5_01.19
Sequence Completeness      -
NCBI accession of oriT (coordinates [strand])   NZ_CP043516 (44216..44305 [+], 90 nt)
oriT length   90 nt
IRs (inverted repeats)      34..41, 44..51  (AGTGCGAT..ATCGCACT)
Location of nic site      62..63
Conserved sequence flanking the
  nic site    		  GGTGTATAGC
Note   Predicted by oriTfinder 2.0

  oriT sequence  


Download         Length: 90 nt

>oriT_pIMPIncN3_57kb
TATTTCATTTTTTGTTTTTTTAATCAGTTAGTTAGTGCGATTCATCGCACTGCGTTAGGTGTATAGCAGGTTAAGGGCTAAAAAATAATC

Visualization of oriT structure

  oriT secondary structure

Predicted by RNAfold.

Download structure file


Relaxase

ID   11646 GenBank   WP_238337819
Name   mobF_FZO55_RS26490_pIMPIncN3_57kb insolico UniProt ID   _
Length   606 a.a. PDB ID   
Note   Predicted by oriTfinder 2.0

  Relaxase protein sequence


Download         Length: 606 a.a.        Molecular weight: 66540.30 Da        Isoelectric Point: 9.6448

>WP_238337819.1 MobF family relaxase [Enterobacter kobei]
MLDITTITRIDIASVVGYYADAKDDYYSKDSSFTAWHGQGAEALGLSGEVSSQRFKELLVGEIDPFTQMK
RSSGDATKERLGYDLTFSAPKGVSMQALIHGDKAIIAAHEKAVAAAVQEAEKLAQARTTHAGKTITQNTG
NLVVASFRHETSRALDPELHTHAFVMNMTQRGDGQWRALKNDELMRAKMHLGDVYKQELALELTKAGYEL
RYNNKNNTFDMAHFTDEQIRGFSRRSAQIEEGLAAMGLTRETADAQTKSRVSMATRDKKTDDYSREDIHK
EWVNRANELGIDFNDLSWAGKGKDGPQTSMNTVPNFTSPEVKADRAIQFALKSLSERDASFDQSRLLAVA
NKKAIGQASKQDIEAAYYRAVAKGAIIEGEARYTTTTDQRSKDAPPLPAMTKAEWQAQLTDKKGMNNDQA
KALVAEGIKSGRLKKTSHRVTTVEGIRVEKAVLNIEARGRGKVIAALTSEQVTAQLVGKTLKAEQQKSVV
DICTTTDRFVAAHGFAGTGKSYMTMSAKAVLESQGFNVTALAPYGTQKKSLEDEGMPARTVAAFVKAKDK
KIDEKSVVFIDEAGVIPARQMKVLMETIEKAGARAVFWATPHRQKP

  Protein domains


Predicted by InterproScan.

AAA domain (AAA_30)

(480-597)

TrwC relaxase (TrwC)

(16-288)


  Protein structure



No available structure.




T4CP

ID   13632 GenBank   WP_032493179
Name   t4cp2_FZO55_RS25835_pIMPIncN3_57kb insolico UniProt ID   _
Length   510 a.a. PDB ID   _
Note   Predicted by oriTfinder 2.0

  T4CP protein sequence


Download         Length: 510 a.a.        Molecular weight: 57912.96 Da        Isoelectric Point: 9.5127

>WP_032493179.1 MULTISPECIES: type IV secretion system DNA-binding domain-containing protein [Enterobacterales]
MTEQERGLIFLCSLLGPIPFVWFITAKFVYGINSETAHYLVPYLLKNTFNLWPLWVAIIAGAVIGLCGFI
GFIIYDKGRVYKGPRFKRVLRGTKLVRASSLAEKTKERDHKQLTVAGVPVPTEYETLHFSIGGATGTGKS
TIIKEAIYSSLKRGTDKRIVLDPNGEYVETFYRPGDVILSPYDKRSPGWSMFNEIRDDFDYDLYAVSIIQ
ESKERSAEEWYRYGRLIYREVSKKLLTLKRSPTMEEVMHWCTIVENEKLKEFVKGTPAEALFIKGAERAT
GSARFVLGDKLAPHLKMPAGDFSLRDWLEDDKPGTLFITWQEQMKKSLNPLISCWLDIMFSSLLGMGKRD
QRTLFFIDELESLEYLPNLQDLLTKGRKSGASVWAGYQTYSQLIERYGINIAETLLGSMRSGIVMGSSRL
GKETLEQMSRALGEIEGEVEHKQGNPFQAGIGNRPRIETRTVRAVTPTEISELKNLTGYVYFPGDLPVGK
FKTKHVQYTRKNPVPGIIMR

  Protein domains


Predicted by InterproScan.

Type IV secretion-system coupling protein DNA-binding domain (TrwB_AAD_bind)

(113-492)

  Protein structure



No available structure.




T4SS

T4SS were predicted by using oriTfinder2.

Region 1: 4415..14691

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
FZO55_RS25545 (FZO55_25545) 45..1173 - 1129 Protein_0 IS3-like element ISSen4 family transposase -
FZO55_RS25550 (FZO55_25550) 1403..1735 + 333 WP_032493174 hypothetical protein -
FZO55_RS25555 (FZO55_25555) 1922..2530 + 609 WP_000275218 recombinase family protein -
FZO55_RS25560 (FZO55_25560) 2527..2712 - 186 WP_024562121 ribbon-helix-helix domain-containing protein -
FZO55_RS25565 (FZO55_25565) 2997..3305 - 309 WP_000733764 hypothetical protein -
FZO55_RS25570 (FZO55_25570) 3309..3647 - 339 WP_032493175 hypothetical protein -
FZO55_RS25575 (FZO55_25575) 3644..3994 - 351 WP_024562120 hypothetical protein -
FZO55_RS25580 (FZO55_25580) 3998..4315 - 318 WP_000960955 H-NS family nucleoid-associated regulatory protein -
FZO55_RS25585 (FZO55_25585) 4415..5197 + 783 WP_024562119 lytic transglycosylase domain-containing protein virB1
FZO55_RS25595 (FZO55_25595) 5444..5740 + 297 WP_015060075 TrbC/VirB2 family protein virB2
FZO55_RS25600 (FZO55_25600) 5780..6097 + 318 WP_000462629 type IV secretion system protein VirB3 virB3
FZO55_RS25605 (FZO55_25605) 6098..8671 + 2574 WP_040115085 VirB4 family type IV secretion/conjugal transfer ATPase virb4
FZO55_RS25610 (FZO55_25610) 8687..9388 + 702 WP_014014949 type IV secretion system protein virB5
FZO55_RS25615 (FZO55_25615) 9398..9640 + 243 WP_000858958 EexN family lipoprotein -
FZO55_RS25620 (FZO55_25620) 9657..10694 + 1038 WP_014014948 type IV secretion system protein virB6
FZO55_RS25630 (FZO55_25630) 10913..11608 + 696 WP_001208352 type IV secretion system protein virB8
FZO55_RS25635 (FZO55_25635) 11619..12506 + 888 WP_000758230 TrbG/VirB9 family P-type conjugative transfer protein virB9
FZO55_RS25640 (FZO55_25640) 12506..13639 + 1134 WP_000101920 type IV secretion system protein VirB10 virB10
FZO55_RS25645 (FZO55_25645) 13690..14691 + 1002 WP_001076634 ATPase, T2SS/T4P/T4SS family virB11
FZO55_RS25650 (FZO55_25650) 14703..15215 + 513 WP_029697418 phospholipase D family protein -
FZO55_RS25655 (FZO55_25655) 15724..16728 - 1005 WP_000427620 IS110-like element IS4321 family transposase -

Host bacterium

ID   18770 GenBank   NZ_CP043516
Plasmid name   pIMPIncN3_57kb Incompatibility group   IncN3
Plasmid size   56515 bp Coordinate of oriT [Strand]   44216..44305 [+]
Host baterium   Enterobacter kobei strain EB_P8_L5_01.19

Cargo genes

Drug resistance gene   blaIMP-70, qacE, sul1, qnrA1
Virulence gene   -
Metal resistance gene   merE, merD, merA, merC, merP, merT, merR
Degradation gene   -
Symbiosis gene   -
Anti-CRISPR   -