Detailed information of oriT
oriT
The information of the oriT region
| oriTDB ID | 118303 |
| Name | oriT_pP47-61  |
| Organism | Enterococcus faecium strain P47 |
| Sequence Completeness | - |
| NCBI accession of oriT (coordinates [strand]) | NZ_CP091102 (35669..35698 [+], 30 nt) |
| oriT length | 30 nt |
| IRs (inverted repeats) | _ |
| Location of nic site | _ |
| Conserved sequence flanking the nic site |
_ |
| Note | Predicted by oriTfinder 2.0 |
oriT sequence
Download Length: 30 nt
>oriT_pP47-61
AACGTAAGTTAGTGGGTATAAGTGCGCCCT
AACGTAAGTTAGTGGGTATAAGTGCGCCCT
Visualization of oriT structure
oriT secondary structure
Predicted by RNAfold.
Download structure file
Relaxase
| ID | 11624 | GenBank | WP_233701194 |
| Name | mobQ_L1881_RS12780_pP47-61 |
UniProt ID | _ |
| Length | 661 a.a. | PDB ID | |
| Note | Predicted by oriTfinder 2.0 | ||
Relaxase protein sequence
Download Length: 661 a.a. Molecular weight: 77313.36 Da Isoelectric Point: 9.2532
>WP_233701194.1 MULTISPECIES: MobQ family relaxase [Lactobacillales]
MAIFHLSMTIAKREGGKRSLIAMASYRSGEKLYSELYEKTNLYNHRTVKPEAFILKPDYVPNEFLDRQTL
WNKMELAEKSPNAQLCREVNVALPIELNNSDQRMLIEDFVKDNFVSEGMIADVAIHRDDENNPHAHIMLT
MREVDSEGNILNKRKRIPKLDENGNQIFNEKGQRVTVSIKTNDWDRKSLVSEIRKDWADKVNQYLKDRNI
DQQITEKSHAELGKKELPTIHEGFYSKKLEDKGVISELKRKNLEIQSYNDILAELDKLESQDKVLKQDQN
FTLKFEKTFSPLEKGELKNLSKELKLFINDENIDKRIGELKRWENSLIFNNKMEIQKQRLMLSKISSERD
MLTKANEILDKQAERFFKKSYPSLNIDKFSNHEVRAMVNETIFRKQLLNKDQLAEVIYNERVIEKEESKK
IFKEKPFQTSRYLDSKIKQVEDSITKENNPERKEILSIKKEKLIGIKQGLIEYVQSEVERKFDKNVSIDS
VIEGEMLLAKADYYKTTDFSKVEGVARFSSEEINSMLEQSKGFLTNIQTVKIPNDCQGVFFVQDSMKYID
ELSPLAKQNLKKVVNRNAYLPDSDKIELSKEIENTNKDQSQELDKDVPEKNEVTVKMFQFAKSINRLLSG
NQLQKKRNLDKLIKQTKAKENQSLQRNIPLR
MAIFHLSMTIAKREGGKRSLIAMASYRSGEKLYSELYEKTNLYNHRTVKPEAFILKPDYVPNEFLDRQTL
WNKMELAEKSPNAQLCREVNVALPIELNNSDQRMLIEDFVKDNFVSEGMIADVAIHRDDENNPHAHIMLT
MREVDSEGNILNKRKRIPKLDENGNQIFNEKGQRVTVSIKTNDWDRKSLVSEIRKDWADKVNQYLKDRNI
DQQITEKSHAELGKKELPTIHEGFYSKKLEDKGVISELKRKNLEIQSYNDILAELDKLESQDKVLKQDQN
FTLKFEKTFSPLEKGELKNLSKELKLFINDENIDKRIGELKRWENSLIFNNKMEIQKQRLMLSKISSERD
MLTKANEILDKQAERFFKKSYPSLNIDKFSNHEVRAMVNETIFRKQLLNKDQLAEVIYNERVIEKEESKK
IFKEKPFQTSRYLDSKIKQVEDSITKENNPERKEILSIKKEKLIGIKQGLIEYVQSEVERKFDKNVSIDS
VIEGEMLLAKADYYKTTDFSKVEGVARFSSEEINSMLEQSKGFLTNIQTVKIPNDCQGVFFVQDSMKYID
ELSPLAKQNLKKVVNRNAYLPDSDKIELSKEIENTNKDQSQELDKDVPEKNEVTVKMFQFAKSINRLLSG
NQLQKKRNLDKLIKQTKAKENQSLQRNIPLR
Protein domains
Predicted by InterproScan.
Protein structure
No available structure.
T4CP
| ID | 13608 | GenBank | WP_002325630 |
| Name | t4cp2_L1881_RS12735_pP47-61 |
UniProt ID | _ |
| Length | 551 a.a. | PDB ID | _ |
| Note | Predicted by oriTfinder 2.0 | ||
T4CP protein sequence
Download Length: 551 a.a. Molecular weight: 63114.14 Da Isoelectric Point: 4.7040
>WP_002325630.1 MULTISPECIES: VirD4-like conjugal transfer protein, CD1115 family [Bacilli]
MSSLLAESDGLILGKFQSGNVVIQPEDSKISNRNIFVVGGPGSFKTQSYVLPNVVNNRSTSIVVTDPKGE
IYELTNEIKKAQGFKTVVINFKDFLLSSRYNPLLYIRKSNDTNKIANVIVSAKNDPKRKDFWFNAQLNLL
NTLIKYVYFEYEPSARTIEGILDFLEEFDPRYNEEGVSELDEQFERLPDGHEAKRSYYLGFRQAQSEARP
NIVISLLTTLQDFVDKEVAEFTSTNDFFFEELGTSKICLYVLISPLDRTWDGLVNLFFQQMFTELYFLGD
KHNAKLPVPLVMLLDEFVNLGYFPTYENFLATCRGYRISVSTILQSLPQGFELYGDKKFKAIIGNHAIKI
CLGGVEETTAEYFSRQVNDTTIKVYTGGTSESKTSAKTTNRSGSKSESYGYQKRRLITEGEVINLQQEEN
GRKSIVLIDGKPYMLRKTPQFELFGNLLKKHEISQQDYISSQTEFAKEYIEELEKQHKQRKVQLASAPIF
HEKKQEDDLNKKEINLPEQPIESEVTEEEEEKELLMSDILSSFDEGIEENTTNNDNSELPF
MSSLLAESDGLILGKFQSGNVVIQPEDSKISNRNIFVVGGPGSFKTQSYVLPNVVNNRSTSIVVTDPKGE
IYELTNEIKKAQGFKTVVINFKDFLLSSRYNPLLYIRKSNDTNKIANVIVSAKNDPKRKDFWFNAQLNLL
NTLIKYVYFEYEPSARTIEGILDFLEEFDPRYNEEGVSELDEQFERLPDGHEAKRSYYLGFRQAQSEARP
NIVISLLTTLQDFVDKEVAEFTSTNDFFFEELGTSKICLYVLISPLDRTWDGLVNLFFQQMFTELYFLGD
KHNAKLPVPLVMLLDEFVNLGYFPTYENFLATCRGYRISVSTILQSLPQGFELYGDKKFKAIIGNHAIKI
CLGGVEETTAEYFSRQVNDTTIKVYTGGTSESKTSAKTTNRSGSKSESYGYQKRRLITEGEVINLQQEEN
GRKSIVLIDGKPYMLRKTPQFELFGNLLKKHEISQQDYISSQTEFAKEYIEELEKQHKQRKVQLASAPIF
HEKKQEDDLNKKEINLPEQPIESEVTEEEEEKELLMSDILSSFDEGIEENTTNNDNSELPF
Protein domains
Predicted by InterproScan.
Protein structure
No available structure.
T4SS
T4SS were predicted by using oriTfinder2.
Region 1: 13255..33203
| Locus tag | Coordinates | Strand | Size (bp) | Protein ID | Product | Description |
|---|---|---|---|---|---|---|
| L1881_RS12655 (L1881_12655) | 8410..9006 | - | 597 | WP_005237668 | PBECR4 domain-containing protein | - |
| L1881_RS12660 (L1881_12660) | 9223..10347 | - | 1125 | WP_235692367 | AAA family ATPase | - |
| L1881_RS12665 (L1881_12665) | 10349..11074 | - | 726 | WP_016630100 | DUF6414 family protein | - |
| L1881_RS12670 (L1881_12670) | 11087..11551 | - | 465 | WP_235692368 | toll/interleukin-1 receptor domain-containing protein | - |
| L1881_RS12675 (L1881_12675) | 11637..12317 | - | 681 | WP_000191454 | IS6-like element ISEnfa1 family transposase | - |
| L1881_RS12680 (L1881_12680) | 12373..13152 | - | 780 | Protein_17 | IS256 family transposase | - |
| L1881_RS12685 (L1881_12685) | 13255..14130 | - | 876 | WP_235692369 | LPXTG cell wall anchor domain-containing protein | prgC |
| L1881_RS12690 (L1881_12690) | 14543..17641 | - | 3099 | WP_002301650 | HsdR family type I site-specific deoxyribonuclease | - |
| L1881_RS12695 (L1881_12695) | 17644..18849 | - | 1206 | WP_002325634 | restriction endonuclease subunit S | - |
| L1881_RS12700 (L1881_12700) | 18846..20393 | - | 1548 | WP_002301648 | type I restriction-modification system subunit M | - |
| L1881_RS12705 (L1881_12705) | 20886..21131 | - | 246 | WP_002301647 | hypothetical protein | - |
| L1881_RS12710 (L1881_12710) | 21477..21755 | + | 279 | WP_002301646 | hypothetical protein | - |
| L1881_RS12715 (L1881_12715) | 21815..22168 | - | 354 | WP_002301645 | hypothetical protein | - |
| L1881_RS12720 (L1881_12720) | 22197..23165 | - | 969 | WP_002301644 | hypothetical protein | - |
| L1881_RS12725 (L1881_12725) | 23182..24114 | - | 933 | WP_002325632 | conjugal transfer protein TrbL family protein | trsL |
| L1881_RS12730 (L1881_12730) | 24116..25039 | - | 924 | WP_212481474 | hypothetical protein | - |
| L1881_RS12735 (L1881_12735) | 25057..26712 | - | 1656 | WP_002325630 | VirD4-like conjugal transfer protein, CD1115 family | - |
| L1881_RS12740 (L1881_12740) | 26705..27136 | - | 432 | WP_002301637 | hypothetical protein | - |
| L1881_RS12745 (L1881_12745) | 27141..27692 | - | 552 | WP_021109241 | hypothetical protein | - |
| L1881_RS12750 (L1881_12750) | 27705..28814 | - | 1110 | WP_021109240 | lysozyme family protein | orf14 |
| L1881_RS12755 (L1881_12755) | 28836..30188 | - | 1353 | WP_104659242 | conjugal transfer protein TraF | trsF |
| L1881_RS12760 (L1881_12760) | 30202..32163 | - | 1962 | WP_231428364 | VirB4 family type IV secretion system protein | virb4 |
| L1881_RS12765 (L1881_12765) | 32174..32803 | - | 630 | WP_104888277 | hypothetical protein | trsD |
| L1881_RS12770 (L1881_12770) | 32820..33203 | - | 384 | WP_001083514 | glycosyl transferase | trsC |
| L1881_RS12775 (L1881_12775) | 33222..33554 | - | 333 | WP_000713503 | CagC family type IV secretion system protein | - |
| L1881_RS12780 (L1881_12780) | 33578..35563 | - | 1986 | WP_233701194 | MobQ family relaxase | - |
| L1881_RS12785 (L1881_12785) | 35855..36154 | + | 300 | WP_002325624 | hypothetical protein | - |
| L1881_RS12790 (L1881_12790) | 36157..36414 | + | 258 | WP_010717195 | hypothetical protein | - |
| L1881_RS12795 (L1881_12795) | 36444..36620 | - | 177 | WP_181728217 | hypothetical protein | - |
Host bacterium
| ID | 18736 | GenBank | NZ_CP091102 |
| Plasmid name | pP47-61 | Incompatibility group | - |
| Plasmid size | 61338 bp | Coordinate of oriT [Strand] | 35669..35698 [+] |
| Host baterium | Enterococcus faecium strain P47 |
Cargo genes
| Drug resistance gene | erm(A), optrA, fexA, erm(B), fosB3 |
| Virulence gene | - |
| Metal resistance gene | - |
| Degradation gene | - |
| Symbiosis gene | - |
| Anti-CRISPR | - |