Detailed information of oriT

oriT

The information of the oriT region


oriTDB ID   118293
Name   oriT_FDAARGOS_1081|unnamed in_silico
Organism   Serratia liquefaciens strain FDAARGOS_1081
Sequence Completeness      -
NCBI accession of oriT (coordinates [strand])   NZ_CP068149 (106113..106195 [+], 83 nt)
oriT length   83 nt
IRs (inverted repeats)     _
Location of nic site      _
Conserved sequence flanking the
  nic site    		  _
Note   Predicted by oriTfinder 2.0

  oriT sequence  


Download         Length: 83 nt

>oriT_FDAARGOS_1081|unnamed
ACGGGACCAGATGTGTTTTGTAGCACCGCCTGCACGCAGTCGGTTCAACAAAACGCTGGGTCAGGGCAGAGCCCCGACACCCC

Visualization of oriT structure

  oriT secondary structure

Predicted by RNAfold.

Download structure file


Relaxase

ID   11622 GenBank   WP_128865578
Name   traI_I6I38_RS25355_FDAARGOS_1081|unnamed insolico UniProt ID   _
Length   651 a.a. PDB ID   
Note   Predicted by oriTfinder 2.0

  Relaxase protein sequence


Download         Length: 651 a.a.        Molecular weight: 73461.66 Da        Isoelectric Point: 7.2603

>WP_128865578.1 TraI/MobA(P) family conjugative relaxase [Serratia liquefaciens]
MIIRIPEKRRDGKSSFLQLVAYTVVRDEDKPDTPLEPEHPGWRRPRSKDDIFNHLVDYISRNGDADLQQI
MHADPHGHTQVMFDGVMCETNCFNITTASVEMNAVALQNTRCKDPVLHYFLSWPETDNPSTEHIFDSVRH
SLSALGMSEHQYVAAIHTDTNNIHCHVAANRIHPETYKAADDSFTRIRLQQAARELELKYNWTPTNGFFA
VNEQGEIVRSKREKSLTPSGAKALEYYADVESLHTYAVTECGFKIDEAMADPNLTWKDVHRILVNAGLAL
KPKGKGLAIYSIDNPELPPVKASSVHPDLTLGCLEKDLGLFQPLENVGTYTKDHTTTATDAIVHEFRYEP
TLHARDLTARLERRLARADARADLKARYQAYKHSWKRPRLDASVVKRRYQNESKRFAWQKARARVAVGDP
LLRKLTYHIIEVERMKAMAALRLAVKEERAAFKADPANRRLSYREWVEQQALSHDQAAIAQLRAFAYRMK
RTQRTAPISTNSIVCAVADDTPAFALEGYATQVTRDGTVQYVRDGCVELQDKGERIEVGNHRVNEGEHIA
GGMALAENKSGEHLKFEGESAFVQQACSLVRWFNEGGDTPLPLSDPQQRIMAGYDASSQAISTGEGQHKL
SAAEQPDVEQSKPRSGYRPQQ

  Protein domains


Predicted by InterproScan.

Large polyvalent protein-associated domain 7 (LPD7)

(519-589)

Relaxase/Mobilisation nuclease domain (Relaxase)

(53-318)


  Protein structure



No available structure.




T4CP

ID   13606 GenBank   WP_236591436
Name   trbC_I6I38_RS25370_FDAARGOS_1081|unnamed insolico UniProt ID   _
Length   725 a.a. PDB ID   _
Note   Predicted by oriTfinder 2.0

  T4CP protein sequence


Download         Length: 725 a.a.        Molecular weight: 81651.39 Da        Isoelectric Point: 8.1679

>WP_236591436.1 F-type conjugative transfer protein TrbC [Serratia liquefaciens]
MSRPVEVDPRRVRRPLGYTFINDALLSPMGVQLSLLAGLIVGFVMPATLLLTVPGLLLLVMLFADRPFRM
PLRMPTDIGGMDLTTEREMPKYRKGLGGFFRYVVRTRKYMPAAGVMCLGYARGKGLARELWLTLDDALRH
MLLLATTGSGKTEALLSVFLNSICWGRGICYSDGKGQNTLAFAMWSLARRFGREDDFYVLNFMTGGTDKL
LQLLLNDKKRQPSNTINLFGTANTTFIIQLMESMLPPAGSGDQGWQDKAKSMLSALVYAIYYKCKREKRR
ISQKVIQEYLPLRKLADLYQEAKRDGWHREAYNPLENYFNTLAGFRIELINKPSEWEQGVYDQHGYLIQQ
FNRMLTMFNDLYGHIFSTDAGDIDIEDVLHNDRILCTTIPALELSKGEASNIGKLYISAIRMTMARDLGC
ELEGMMNDVLIVKKYSGKFPYPIAMDELGAYFGPGMDNLASQMRSLGYMLIVSAQDIQRFIAEHKGEYMT
VNANLLTKWFMTLQDEKDTFDLARITGGKGYYAELGSVEQAGGFITPNYEDAANNYIREKDRLDLGDLKD
MSPGEGMISFKSALVPSNAIYIPDDQKMTSSLPMRINRFIDVETPTEAELFTLNPSLKRKLPPTAQEIDG
ILQRLDQAMDTTTSAGMMDPVLKRVAAVALDLDNRADVSYSPTQRGVLLFEAAREALHQNKRNWRQLPAP
PKPIRVSKEVAQSLANAGTNEITFR

  Protein domains



No domain identified.


  Protein structure



No available structure.




T4SS

T4SS were predicted by using oriTfinder2.

Region 1: 111662..140494

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
I6I38_RS25360 (I6I38_25365) 108567..108890 - 324 WP_201896509 hypothetical protein -
I6I38_RS25365 (I6I38_25370) 108900..109436 - 537 WP_201896510 phospholipase D family protein -
I6I38_RS25370 (I6I38_25375) 109447..111624 - 2178 WP_236591436 F-type conjugative transfer protein TrbC -
I6I38_RS25375 (I6I38_25380) 111662..112675 - 1014 WP_201896511 hypothetical protein trbB
I6I38_RS25380 (I6I38_25385) 112689..113948 - 1260 WP_201896512 conjugal transfer protein TrbA trbA
I6I38_RS25385 (I6I38_25390) 114142..114720 - 579 WP_128865581 hypothetical protein -
I6I38_RS25390 (I6I38_25395) 114717..116861 - 2145 WP_201896513 DotA/TraY family protein traY
I6I38_RS25395 (I6I38_25400) 116926..117489 - 564 WP_128865583 conjugal transfer protein TraX -
I6I38_RS25400 (I6I38_25405) 117482..118708 - 1227 WP_201896514 conjugal transfer protein TraW traW
I6I38_RS25405 (I6I38_25410) 118849..121923 - 3075 WP_201896515 ATP-binding protein traU
I6I38_RS25410 (I6I38_25415) 121926..122531 - 606 WP_201896516 hypothetical protein traT
I6I38_RS25415 (I6I38_25420) 122616..123017 - 402 WP_127147005 DUF6750 family protein traR
I6I38_RS25420 (I6I38_25425) 123033..123563 - 531 WP_010895872 conjugal transfer protein TraQ traQ
I6I38_RS25425 (I6I38_25430) 123572..124315 - 744 WP_201896517 hypothetical protein traP
I6I38_RS25430 (I6I38_25435) 124315..125547 - 1233 WP_127147003 conjugal transfer protein TraO traO
I6I38_RS25435 (I6I38_25440) 125552..126559 - 1008 WP_127147002 DotH/IcmK family type IV secretion protein traN
I6I38_RS25440 (I6I38_25445) 126562..127236 - 675 WP_127147001 DotI/IcmL/TraM family protein traM
I6I38_RS25445 (I6I38_25450) 127329..127820 - 492 WP_127147000 hypothetical protein traL
I6I38_RS25450 (I6I38_25455) 127830..131063 - 3234 WP_201896518 LPD7 domain-containing protein -
I6I38_RS25455 (I6I38_25460) 131060..131197 - 138 WP_201896519 hypothetical protein -
I6I38_RS25460 (I6I38_25465) 131206..131472 - 267 WP_201896520 IcmT/TraK family protein traK
I6I38_RS25465 (I6I38_25470) 131472..132629 - 1158 WP_201896521 plasmid transfer ATPase TraJ virB11
I6I38_RS25470 (I6I38_25475) 132660..133448 - 789 WP_201896522 type IV secretory system conjugative DNA transfer family protein traI
I6I38_RS25475 (I6I38_25480) 133445..133909 - 465 WP_201896523 DotD/TraH family lipoprotein -
I6I38_RS25480 (I6I38_25485) 134202..134603 + 402 WP_236591437 DapH/DapD/GlmU-related protein -
I6I38_RS25485 (I6I38_25490) 134676..136061 - 1386 WP_236591438 shufflon system plasmid conjugative transfer pilus tip adhesin PilV -
I6I38_RS25490 (I6I38_25495) 136058..136726 - 669 WP_201896525 prepilin peptidase -
I6I38_RS25495 (I6I38_25500) 136726..137211 - 486 WP_048795524 lytic transglycosylase domain-containing protein virB1
I6I38_RS25500 (I6I38_25505) 137266..137856 - 591 WP_201896526 type 4 pilus major pilin -
I6I38_RS25505 (I6I38_25510) 137892..139007 - 1116 WP_201896527 type II secretion system F family protein -
I6I38_RS25510 (I6I38_25515) 139004..140494 - 1491 WP_201896528 GspE/PulE family protein virB11
I6I38_RS25515 (I6I38_25520) 140500..141084 - 585 WP_127146991 type IV pilus biogenesis protein PilP -
I6I38_RS25520 (I6I38_25525) 141071..142375 - 1305 WP_201896529 type 4b pilus protein PilO2 -
I6I38_RS25525 (I6I38_25530) 142379..144013 - 1635 WP_127146989 PilN family type IVB pilus formation outer membrane protein -
I6I38_RS25530 (I6I38_25535) 144037..144462 - 426 WP_127146988 type IV pilus biogenesis protein PilM -
I6I38_RS25535 (I6I38_25540) 144462..145493 - 1032 WP_201896530 toxin co-regulated pilus biosynthesis Q family protein -

Host bacterium

ID   18726 GenBank   NZ_CP068149
Plasmid name   FDAARGOS_1081|unnamed Incompatibility group   -
Plasmid size   187192 bp Coordinate of oriT [Strand]   106113..106195 [+]
Host baterium   Serratia liquefaciens strain FDAARGOS_1081

Cargo genes

Drug resistance gene   -
Virulence gene   pilW
Metal resistance gene   -
Degradation gene   -
Symbiosis gene   -
Anti-CRISPR   -