Detailed information of oriT
oriT
The information of the oriT region
oriTDB ID | 118289 |
Name | oriT_83_pKSR100 |
Organism | Shigella flexneri 3a isolate 83 |
Sequence Completeness | - |
NCBI accession of oriT (coordinates [strand]) | NZ_LR878367 (59171..59522 [-], 352 nt) |
oriT length | 352 nt |
IRs (inverted repeats) | 192..198, 206..212 (TATAAAA..TTTTATA) 104..109, 119..124 (TTTAAA..TTTAAA) 93..98, 106..111 (GATTTA..TAAATC) 40..47, 50..57 (GCAAAAAC..GTTTTTGC) 4..11, 16..23 (TTGGTGGT..ACCACCAA) |
Location of nic site | _ |
Conserved sequence flanking the nic site |
_ |
Note | Predicted by oriTfinder 2.0 |
oriT sequence
Download Length: 352 nt
AGGTTGGTGGTTCTCACCACCAAAAGCACCACACACTACGCAAAAACAAGTTTTTGCTGATTTATCTTTAATATCATTGAGTTGTATTTGTGGATTTATATTGTTTAAATCTGATTTTTTTAAAGCAGCGTCGTTAACGCAGCTACAGCAACGCGCCGACACCGCTTTGTAGGGGTGGTACTGACTATTTTTATAAAAAACATTATTTTATATTAGGGGTGCTGCTAGCGGCGCGGTGTGTTTTTTTATAGGATACCGTCAGGGGCGCTGCTAGCGGTGCGTCCCTGTTTGCACTATGAATTCTAGTGTTTCGAAATTAACTTTGTTTTATGTTTAAAAAAGGTAATATCTA
Visualization of oriT structure
oriT secondary structure
Predicted by RNAfold.
Download structure fileRelaxase
ID | 11618 | GenBank | WP_152030378 |
Name | traI_JMV05_RS24400_83_pKSR100 | UniProt ID | _ |
Length | 1756 a.a. | PDB ID | |
Note | Predicted by oriTfinder 2.0 |
Relaxase protein sequence
Download Length: 1756 a.a. Molecular weight: 191501.96 Da Isoelectric Point: 5.6388
MLSFSVVKSAGSAGNYYTDKDNYYVLGSMGERWAGQGAEQLGLQGSVDKDVFTRLLEGRLPDGADLSRMQ
DGSNKHRPGYDLTFSAPKSVSMMAMLGGDKRLIDAHNQAVDFAVRQVEALASTRVMTDGQSETVLTGNLV
MALFNHDTSRDQDPQLHTHVVVANVTQHNGEWKTLSSDKVGKTGFSENVLANRIAFGKIYQSELRQRVEA
LGYETEVVGKHGMWEMPGVPVEAFSSRSQAIREAVGEDASLKSRDVAALDTRKSKQHVDPEIRMAEWMQT
LKETGFDIRAYRDAADQRAEIRTQAPGPASQDGPDVQQAVTQAIAGLSERKVQFTYTDVLARTVGILPPE
NGVIERARAGIDEAISREQLIPLDREKGLFTSGIHVLDELSVRALSRDIMKQNRVTVHPEKSVPRTAGYS
DAVSVLAQDRPSLAIVSGQGGAAGQRERVAELVMMAREQGREVQIIAADRRSQMNLKQDERLSGELITGR
RQLLEGMAFTPGSTVIVDQGEKLSLKETLTLLDGAARHNVQVLITDSGQRTGTGSALMAMKDAGVNTYRW
QGGEQRPATIISEPDRNVRYARLAGDFAASVKAGEESVAQVSGVREQAILTQAIRSELKTQGVLGHPEVT
MTALSPVWLDSRSRYLRDMYRPGMVMEQWNPETRSHDRYVIDRVTAQSHSLTLRDAQGETQVVRISSLDS
SWSLFRPEKMPVADGERLRVTGKIPGLRVSGGDRLQVASVSEDAMTVVVPGRAEPATLPVADSPFTALKL
ENGWVETPGHSVSDSATVFASVTQMAMDNATLNGLARSGRDVRLYSSLDETRTAEKLARHPSFTVVSEQI
KARAGETLLETAISLQKAGLHTPAQQAIHLALPVLESKNLAFSMVDLLTEAKSFAAEGTGFTELGGEINA
QIKRGDLLYVDVAKGYGTGLLVSRASYEAEKSILRHILEGKEAVTPLMERVPGELMETLTSGQRAATRMI
LETSDRFTVVQGYAGVGKTTQFRAVMSAVNMLPESERPRVVGLGPTHRAVGEMRSAGVDAQTLASFLHDT
QLQQRSGETPDFSNTLFLLDESSMVGNTDMARAYALIAAGGGRAVASGDTDQLQAIAPGQPFRLQQTRSA
ADVAIMKEIVRQTPELREAVYSLINRDVERALSGLESVKPSQVPRLEGAWAPEHSVTEFSHSQEAKLAEA
QQKAMQNGEAFPDVPMTLYEAIVRDYTGRTPEAREQTLIVTHLNEDRRVLNSMIHDAREKAGELGKEQVM
VPVLNTANIRDGELRRLSTWENNPDALALVDNVYHRIAGIGKDDGLITLEDAEGNTRLISPREAVAEGVT
LYTPDTIRVGTGDRMRFTKSDRERGYVANSVWTVTAVSGDSVTLSDGQQTRVIRPGQERAEQHIDLAYAI
TAHGAQGASETFAIALEGTEGNRKLMAGFESAYVALSRMKQHVQVYTDNRQGWTDAINNAVQKGTAHDVF
EPKPDREVMSAQRLFSTARELRDVAAGRAVLRQAGLAGGDSPARFIAPGRKYPQPYVALPAFDRNGRSAG
IWLNPLTTDDGNGLRGFSGEGRVKGSGDAQFVALQGSRNGEGLLADNMQDGVRIARDNPDSGVVVRIAGE
GRPWNPGAITGGRVWGDIPDNSVRPGAGNGEPVTAEVLAQQQAEEAIRRETERRADEIVRKMAGNKPDLP
DGKTEQAVREIAGQERDRTATSERETALPESVLRESQREQEAVREVAQENLLQERLQQMERDMVRDLQKE
KTLGGD
Protein domains
Predicted by InterproScan.
Protein structure
No available structure.
Auxiliary protein
ID | 6711 | GenBank | WP_001151529 |
Name | WP_001151529_83_pKSR100 | UniProt ID | A0A7I0L277 |
Length | 127 a.a. | PDB ID | _ |
Note | Predicted by oriTfinder 2.0 |
Auxiliary protein sequence
Download Length: 127 a.a. Molecular weight: 14497.49 Da Isoelectric Point: 4.8662
MAKVNLYISNDAYEKINAIIEKRRQEGAREKDVSFSATASMLLELGLRVYEAQMERKESAFNQAEFNKLL
LECVVKTQSSVAKILGIESLSPHVSGNPKFEYANMVEDIREKVSAEMERFFPKNDDE
Protein domains
Predicted by InterproScan.
Protein structure
Source | ID | Structure |
---|---|---|
AlphaFold DB | A0A7I0L277 |
T4CP
ID | 13602 | GenBank | WP_128867119 |
Name | traD_JMV05_RS24405_83_pKSR100 | UniProt ID | _ |
Length | 750 a.a. | PDB ID | _ |
Note | Predicted by oriTfinder 2.0 |
T4CP protein sequence
Download Length: 750 a.a. Molecular weight: 85432.30 Da Isoelectric Point: 5.2345
MSFNAKDMTQGGQIASMRIRMFSQIANIMLYCLFIFFWILIGLVLWVKISWQTFINGCIYWWCTSLEGMR
DLIKSQPVYEIQYYGKTFRMNAAQVLHDKYMIWCGEQLWSAFVLATVVALVICLITFFVVSWILGRQGKQ
QSENEVTGGRQLTDNPKDVARMLKKDGKDSDIRIGDLPIIRDSEIQNFCLHGTVSTGKSEVIRRLANYAR
KRGDMVVIYDRSCEFVKSYYDPSIDKILNPLDARCAAWDLWKECLTQPDFDNVANTLIPMGTKEDPFWQG
SGRTIFAEAAYLMRNDPNRSYSKLVDTLLSIKIEKLRTFLRNSPAANLVEEKIEKTAISIRAVLTNYVKA
IRYLQGIEHNGEPFTIRDWMRGVREDQKNGWLFISSNADTHASLKPVVSMWLSIAIRGLLAMGENRNRRV
WFFCDELPTLHKLPDLVEILPEARKFGGCYVFGIQSYAQLEDIYGEKAAATLFDVLNTRAFFRSPSHQIA
EFAAGEIGEKEHLKASLQYSYGADPVRDGVSTGKEMERQTLVSYSDIQSLPDLTCYVTLPGPYPAVKLSL
KYQTRPKVAPEFIPRDINPEMENRLSAVLAAREAEGRQMASLFEPEVASGEDVTQAEQPQQPQQPQQPQQ
PQQPQQPQQPQQPQQPQQPQQPQQSQQPVSSVINDKKSDAGVSVPAGGIEQELKMKPEEEMEQQLPPGIS
ESGEVVDMAAYEAWQQENHPDIQQHMQRREEVNINVHRERGEDVEPGDDF
Protein domains
Predicted by InterproScan.
Protein structure
No available structure.
ID | 13603 | GenBank | WP_000069772 |
Name | traC_JMV05_RS24510_83_pKSR100 | UniProt ID | _ |
Length | 876 a.a. | PDB ID | _ |
Note | Predicted by oriTfinder 2.0 |
T4CP protein sequence
Download Length: 876 a.a. Molecular weight: 99344.15 Da Isoelectric Point: 6.4096
MSNNPLEAVTQAVNSLVTALKLPDESAKANEVLGEMSFPQFSRLLPYRDYNQESGLFMNDTTMGFMLEAI
PINGANKSIVEALDHMLRTKLPRGIPLCIHLMSSQLVGDRIEYGLREFSWSGEQAERFNAITRAYYMKAA
ATQFPLPEGMNLPLTLRHYRVFISYCSPSKKKSRADILEMENLVKIIRASFHGAKITTQTVDAQAFIEIV
GEMINHNPDSLYPKRRQLDPYSDLNYQCVEDSFDLNVRADYLTLGLRENGRNSTARILNFHLARNPEIAF
LWNMADNYSNLLNPEMSISCPFILTLTLVVEDQVKTHSEANLKYMDLEKKSKTSYAKWFPSVEKEAKEWG
ELRQRLGSGQSSVVSYFLNITAFCKDNNETALEVEQDILNSFRKNGFELISPRFNHMRNFLTCLPFMAGK
GLFKQLKEAGVVQRAESFNVANLMPLVADNPLTPAGLLAPTYRNQLAFIDIFFKGMNNTNYNMAVCGTSG
AGKTGLIQPLIRSVLDSGGFAVVFDMGDGYKSLCENMGGVYLDGETLRFNPFANITDIDQSAERVRDQLS
VMASPNGNLDEVHEGLLLQAVRASWLAKENRARIDDVVDFLKNASDSEQYAGSPTIRSRLDEMIVLLDQY
TANGTYGQYFNSDEPSLRDDAKMVVLELGGLEDRPSLLVAVMFSLIIYIENRMYRTPRTLKKLNVIDEGW
RLLDFKNRKVGEFIQKGYRTCRRHTGAYITITQNIVDFDSDKASSAARAAWGNSSYKIILKQSAKEFAKY
NQLFPDQFQPLQRDMIGKFGAAKDQWFSSFLLQVENHSSWHRLFVDPLSRAMYSSDGPDFEFVQQKRREG
LSIHEAVWQLAWKKSGPEMASLEAWLEEHEKYRSVA
Protein domains
Predicted by InterproScan.
Protein structure
No available structure.
T4SS
T4SS were predicted by using oriTfinder2.
Region 1: 30663..60093
Locus tag | Coordinates | Strand | Size (bp) | Protein ID | Product | Description |
---|---|---|---|---|---|---|
JMV05_RS24405 | 30663..32915 | - | 2253 | WP_128867119 | type IV conjugative transfer system coupling protein TraD | prgC |
JMV05_RS24410 | 32966..33703 | - | 738 | WP_012881122 | hypothetical protein | - |
JMV05_RS24415 | 33906..34637 | - | 732 | WP_012881123 | conjugal transfer complement resistance protein TraT | - |
JMV05_RS24420 | 34651..35160 | - | 510 | WP_000628111 | conjugal transfer entry exclusion protein TraS | - |
JMV05_RS24425 | 35157..37982 | - | 2826 | WP_033807963 | conjugal transfer mating-pair stabilization protein TraG | traG |
JMV05_RS24430 | 37979..39352 | - | 1374 | WP_033807962 | conjugal transfer pilus assembly protein TraH | traH |
JMV05_RS24435 | 39339..39731 | - | 393 | WP_000659962 | F-type conjugal transfer protein TrbF | - |
JMV05_RS24440 | 39712..40059 | - | 348 | WP_071571857 | P-type conjugative transfer protein TrbJ | - |
JMV05_RS24445 | 39989..40534 | - | 546 | WP_000059829 | type-F conjugative transfer system pilin assembly thiol-disulfide isomerase TrbB | traF |
JMV05_RS24450 | 40521..40805 | - | 285 | WP_000624107 | type-F conjugative transfer system pilin chaperone TraQ | - |
JMV05_RS24455 | 40886..41221 | + | 336 | WP_001620807 | hypothetical protein | - |
JMV05_RS24460 | 41202..41543 | - | 342 | WP_000556795 | conjugal transfer protein TrbA | - |
JMV05_RS24465 | 41557..42300 | - | 744 | WP_001030371 | type-F conjugative transfer system pilin assembly protein TraF | traF |
JMV05_RS24470 | 42293..42550 | - | 258 | WP_033807961 | conjugal transfer protein TrbE | - |
JMV05_RS24475 | 42577..44481 | - | 1905 | WP_225620370 | type-F conjugative transfer system mating-pair stabilization protein TraN | traN |
JMV05_RS24480 | 44717..45097 | - | 381 | WP_001462111 | hypothetical protein | - |
JMV05_RS24485 | 45094..45732 | - | 639 | WP_033807960 | type-F conjugative transfer system pilin assembly protein TrbC | trbC |
JMV05_RS24490 | 45741..46046 | - | 306 | WP_000224418 | hypothetical protein | - |
JMV05_RS24495 | 46076..47068 | - | 993 | WP_000830849 | conjugal transfer pilus assembly protein TraU | traU |
JMV05_RS24500 | 47065..47697 | - | 633 | WP_001203720 | type-F conjugative transfer system protein TraW | traW |
JMV05_RS24505 | 47694..48080 | - | 387 | WP_000214084 | type-F conjugative transfer system protein TrbI | - |
JMV05_RS24510 | 48077..50707 | - | 2631 | WP_000069772 | type IV secretion system protein TraC | virb4 |
JMV05_RS24515 | 50833..51180 | - | 348 | WP_000836682 | hypothetical protein | - |
JMV05_RS24520 | 51208..51426 | - | 219 | WP_033807959 | hypothetical protein | - |
JMV05_RS24525 | 51525..51998 | - | 474 | WP_033807958 | hypothetical protein | - |
JMV05_RS24530 | 51991..52404 | - | 414 | WP_000549589 | hypothetical protein | - |
JMV05_RS24535 | 52397..52618 | - | 222 | WP_001278683 | conjugal transfer protein TraR | - |
JMV05_RS24540 | 52753..53268 | - | 516 | WP_000809881 | type IV conjugative transfer system lipoprotein TraV | traV |
JMV05_RS24545 | 53265..53585 | - | 321 | WP_012881128 | conjugal transfer protein TrbD | - |
JMV05_RS24550 | 53572..54162 | - | 591 | WP_000002784 | conjugal transfer pilus-stabilizing protein TraP | - |
JMV05_RS24555 | 54152..55579 | - | 1428 | WP_033807957 | F-type conjugal transfer pilus assembly protein TraB | traB |
JMV05_RS24560 | 55579..56307 | - | 729 | WP_001230769 | type-F conjugative transfer system secretin TraK | traK |
JMV05_RS24565 | 56294..56860 | - | 567 | WP_000399780 | type IV conjugative transfer system protein TraE | traE |
JMV05_RS24570 | 56882..57193 | - | 312 | WP_000012113 | type IV conjugative transfer system protein TraL | traL |
JMV05_RS24575 | 57208..57567 | - | 360 | WP_001098994 | type IV conjugative transfer system pilin TraA | - |
JMV05_RS24580 | 57601..57828 | - | 228 | WP_000589556 | conjugal transfer relaxosome protein TraY | - |
JMV05_RS24585 | 57948..58595 | - | 648 | WP_000332523 | transcriptional regulator TraJ family protein | - |
JMV05_RS24590 | 58787..59170 | - | 384 | WP_001151529 | conjugal transfer relaxosome DNA-binding protein TraM | - |
JMV05_RS24595 | 59503..60093 | + | 591 | WP_000252683 | transglycosylase SLT domain-containing protein | virB1 |
JMV05_RS24600 | 60390..61211 | - | 822 | WP_001234445 | DUF932 domain-containing protein | - |
JMV05_RS24605 | 61322..61618 | - | 297 | WP_001272251 | hypothetical protein | - |
JMV05_RS24610 | 61918..62214 | + | 297 | Protein_78 | hypothetical protein | - |
JMV05_RS24615 | 62533..62658 | - | 126 | WP_001372321 | type I toxin-antitoxin system Hok family toxin | - |
JMV05_RS24620 | 62600..62749 | - | 150 | Protein_80 | plasmid maintenance protein Mok | - |
JMV05_RS24625 | 62771..62950 | + | 180 | WP_001334662 | hypothetical protein | - |
JMV05_RS24630 | 62928..63690 | - | 763 | Protein_82 | plasmid SOS inhibition protein A | - |
JMV05_RS24635 | 63687..64121 | - | 435 | WP_000845953 | conjugation system SOS inhibitor PsiB | - |
Host bacterium
ID | 18722 | GenBank | NZ_LR878367 |
Plasmid name | 83_pKSR100 | Incompatibility group | - |
Plasmid size | 72593 bp | Coordinate of oriT [Strand] | 59171..59522 [-] |
Host baterium | Shigella flexneri 3a isolate 83 |
Cargo genes
Drug resistance gene | erm(B), mph(A), blaTEM-1B |
Virulence gene | - |
Metal resistance gene | - |
Degradation gene | - |
Symbiosis gene | - |
Anti-CRISPR | - |