Detailed information of oriT

oriT


The information of the oriT region


oriTDB ID   118289
Name   oriT_83_pKSR100 in_silico
Organism   Shigella flexneri 3a isolate 83
Sequence Completeness      -
NCBI accession of oriT (coordinates [strand])   NZ_LR878367 (59171..59522 [-], 352 nt)
oriT length   352 nt
IRs (inverted repeats)      192..198, 206..212  (TATAAAA..TTTTATA)
 104..109, 119..124  (TTTAAA..TTTAAA)
 93..98, 106..111  (GATTTA..TAAATC)
 40..47, 50..57  (GCAAAAAC..GTTTTTGC)
 4..11, 16..23  (TTGGTGGT..ACCACCAA)
Location of nic site      _
Conserved sequence flanking the
  nic site  
 
 _
Note   Predicted by oriTfinder 2.0

  oriT sequence  


Download         Length: 352 nt

>oriT_83_pKSR100
AGGTTGGTGGTTCTCACCACCAAAAGCACCACACACTACGCAAAAACAAGTTTTTGCTGATTTATCTTTAATATCATTGAGTTGTATTTGTGGATTTATATTGTTTAAATCTGATTTTTTTAAAGCAGCGTCGTTAACGCAGCTACAGCAACGCGCCGACACCGCTTTGTAGGGGTGGTACTGACTATTTTTATAAAAAACATTATTTTATATTAGGGGTGCTGCTAGCGGCGCGGTGTGTTTTTTTATAGGATACCGTCAGGGGCGCTGCTAGCGGTGCGTCCCTGTTTGCACTATGAATTCTAGTGTTTCGAAATTAACTTTGTTTTATGTTTAAAAAAGGTAATATCTA

Visualization of oriT structure

  oriT secondary structure

Predicted by RNAfold.

Download structure file


Relaxase


ID   11618 GenBank   WP_152030378
Name   traI_JMV05_RS24400_83_pKSR100 insolico UniProt ID   _
Length   1756 a.a. PDB ID   
Note   Predicted by oriTfinder 2.0

  Relaxase protein sequence


Download         Length: 1756 a.a.        Molecular weight: 191501.96 Da        Isoelectric Point: 5.6388

>WP_152030378.1 conjugative transfer relaxase/helicase TraI [Shigella flexneri]
MLSFSVVKSAGSAGNYYTDKDNYYVLGSMGERWAGQGAEQLGLQGSVDKDVFTRLLEGRLPDGADLSRMQ
DGSNKHRPGYDLTFSAPKSVSMMAMLGGDKRLIDAHNQAVDFAVRQVEALASTRVMTDGQSETVLTGNLV
MALFNHDTSRDQDPQLHTHVVVANVTQHNGEWKTLSSDKVGKTGFSENVLANRIAFGKIYQSELRQRVEA
LGYETEVVGKHGMWEMPGVPVEAFSSRSQAIREAVGEDASLKSRDVAALDTRKSKQHVDPEIRMAEWMQT
LKETGFDIRAYRDAADQRAEIRTQAPGPASQDGPDVQQAVTQAIAGLSERKVQFTYTDVLARTVGILPPE
NGVIERARAGIDEAISREQLIPLDREKGLFTSGIHVLDELSVRALSRDIMKQNRVTVHPEKSVPRTAGYS
DAVSVLAQDRPSLAIVSGQGGAAGQRERVAELVMMAREQGREVQIIAADRRSQMNLKQDERLSGELITGR
RQLLEGMAFTPGSTVIVDQGEKLSLKETLTLLDGAARHNVQVLITDSGQRTGTGSALMAMKDAGVNTYRW
QGGEQRPATIISEPDRNVRYARLAGDFAASVKAGEESVAQVSGVREQAILTQAIRSELKTQGVLGHPEVT
MTALSPVWLDSRSRYLRDMYRPGMVMEQWNPETRSHDRYVIDRVTAQSHSLTLRDAQGETQVVRISSLDS
SWSLFRPEKMPVADGERLRVTGKIPGLRVSGGDRLQVASVSEDAMTVVVPGRAEPATLPVADSPFTALKL
ENGWVETPGHSVSDSATVFASVTQMAMDNATLNGLARSGRDVRLYSSLDETRTAEKLARHPSFTVVSEQI
KARAGETLLETAISLQKAGLHTPAQQAIHLALPVLESKNLAFSMVDLLTEAKSFAAEGTGFTELGGEINA
QIKRGDLLYVDVAKGYGTGLLVSRASYEAEKSILRHILEGKEAVTPLMERVPGELMETLTSGQRAATRMI
LETSDRFTVVQGYAGVGKTTQFRAVMSAVNMLPESERPRVVGLGPTHRAVGEMRSAGVDAQTLASFLHDT
QLQQRSGETPDFSNTLFLLDESSMVGNTDMARAYALIAAGGGRAVASGDTDQLQAIAPGQPFRLQQTRSA
ADVAIMKEIVRQTPELREAVYSLINRDVERALSGLESVKPSQVPRLEGAWAPEHSVTEFSHSQEAKLAEA
QQKAMQNGEAFPDVPMTLYEAIVRDYTGRTPEAREQTLIVTHLNEDRRVLNSMIHDAREKAGELGKEQVM
VPVLNTANIRDGELRRLSTWENNPDALALVDNVYHRIAGIGKDDGLITLEDAEGNTRLISPREAVAEGVT
LYTPDTIRVGTGDRMRFTKSDRERGYVANSVWTVTAVSGDSVTLSDGQQTRVIRPGQERAEQHIDLAYAI
TAHGAQGASETFAIALEGTEGNRKLMAGFESAYVALSRMKQHVQVYTDNRQGWTDAINNAVQKGTAHDVF
EPKPDREVMSAQRLFSTARELRDVAAGRAVLRQAGLAGGDSPARFIAPGRKYPQPYVALPAFDRNGRSAG
IWLNPLTTDDGNGLRGFSGEGRVKGSGDAQFVALQGSRNGEGLLADNMQDGVRIARDNPDSGVVVRIAGE
GRPWNPGAITGGRVWGDIPDNSVRPGAGNGEPVTAEVLAQQQAEEAIRRETERRADEIVRKMAGNKPDLP
DGKTEQAVREIAGQERDRTATSERETALPESVLRESQREQEAVREVAQENLLQERLQQMERDMVRDLQKE
KTLGGD

  Protein domains


Predicted by InterproScan.

(1434-1557)

(633-712)

(10-283)

(968-1156)

(575-626)


  Protein structure



No available structure.




Auxiliary protein


ID   6711 GenBank   WP_001151529
Name   WP_001151529_83_pKSR100 insolico UniProt ID   A0A7I0L277
Length   127 a.a. PDB ID   _
Note   Predicted by oriTfinder 2.0

  Auxiliary protein sequence


Download         Length: 127 a.a.        Molecular weight: 14497.49 Da        Isoelectric Point: 4.8662

>WP_001151529.1 MULTISPECIES: conjugal transfer relaxosome DNA-binding protein TraM [Enterobacteriaceae]
MAKVNLYISNDAYEKINAIIEKRRQEGAREKDVSFSATASMLLELGLRVYEAQMERKESAFNQAEFNKLL
LECVVKTQSSVAKILGIESLSPHVSGNPKFEYANMVEDIREKVSAEMERFFPKNDDE

  Protein domains


Predicted by InterproScan.

(1-126)


  Protein structure


Source ID Structure
AlphaFold DB A0A7I0L277


T4CP


ID   13602 GenBank   WP_128867119
Name   traD_JMV05_RS24405_83_pKSR100 insolico UniProt ID   _
Length   750 a.a. PDB ID   _
Note   Predicted by oriTfinder 2.0

  T4CP protein sequence


Download         Length: 750 a.a.        Molecular weight: 85432.30 Da        Isoelectric Point: 5.2345

>WP_128867119.1 MULTISPECIES: type IV conjugative transfer system coupling protein TraD [Enterobacteriaceae]
MSFNAKDMTQGGQIASMRIRMFSQIANIMLYCLFIFFWILIGLVLWVKISWQTFINGCIYWWCTSLEGMR
DLIKSQPVYEIQYYGKTFRMNAAQVLHDKYMIWCGEQLWSAFVLATVVALVICLITFFVVSWILGRQGKQ
QSENEVTGGRQLTDNPKDVARMLKKDGKDSDIRIGDLPIIRDSEIQNFCLHGTVSTGKSEVIRRLANYAR
KRGDMVVIYDRSCEFVKSYYDPSIDKILNPLDARCAAWDLWKECLTQPDFDNVANTLIPMGTKEDPFWQG
SGRTIFAEAAYLMRNDPNRSYSKLVDTLLSIKIEKLRTFLRNSPAANLVEEKIEKTAISIRAVLTNYVKA
IRYLQGIEHNGEPFTIRDWMRGVREDQKNGWLFISSNADTHASLKPVVSMWLSIAIRGLLAMGENRNRRV
WFFCDELPTLHKLPDLVEILPEARKFGGCYVFGIQSYAQLEDIYGEKAAATLFDVLNTRAFFRSPSHQIA
EFAAGEIGEKEHLKASLQYSYGADPVRDGVSTGKEMERQTLVSYSDIQSLPDLTCYVTLPGPYPAVKLSL
KYQTRPKVAPEFIPRDINPEMENRLSAVLAAREAEGRQMASLFEPEVASGEDVTQAEQPQQPQQPQQPQQ
PQQPQQPQQPQQPQQPQQPQQPQQSQQPVSSVINDKKSDAGVSVPAGGIEQELKMKPEEEMEQQLPPGIS
ESGEVVDMAAYEAWQQENHPDIQQHMQRREEVNINVHRERGEDVEPGDDF

  Protein domains


Predicted by InterproScan.

(173-560)

(32-128)

  Protein structure



No available structure.



ID   13603 GenBank   WP_000069772
Name   traC_JMV05_RS24510_83_pKSR100 insolico UniProt ID   _
Length   876 a.a. PDB ID   _
Note   Predicted by oriTfinder 2.0

  T4CP protein sequence


Download         Length: 876 a.a.        Molecular weight: 99344.15 Da        Isoelectric Point: 6.4096

>WP_000069772.1 MULTISPECIES: type IV secretion system protein TraC [Enterobacteriaceae]
MSNNPLEAVTQAVNSLVTALKLPDESAKANEVLGEMSFPQFSRLLPYRDYNQESGLFMNDTTMGFMLEAI
PINGANKSIVEALDHMLRTKLPRGIPLCIHLMSSQLVGDRIEYGLREFSWSGEQAERFNAITRAYYMKAA
ATQFPLPEGMNLPLTLRHYRVFISYCSPSKKKSRADILEMENLVKIIRASFHGAKITTQTVDAQAFIEIV
GEMINHNPDSLYPKRRQLDPYSDLNYQCVEDSFDLNVRADYLTLGLRENGRNSTARILNFHLARNPEIAF
LWNMADNYSNLLNPEMSISCPFILTLTLVVEDQVKTHSEANLKYMDLEKKSKTSYAKWFPSVEKEAKEWG
ELRQRLGSGQSSVVSYFLNITAFCKDNNETALEVEQDILNSFRKNGFELISPRFNHMRNFLTCLPFMAGK
GLFKQLKEAGVVQRAESFNVANLMPLVADNPLTPAGLLAPTYRNQLAFIDIFFKGMNNTNYNMAVCGTSG
AGKTGLIQPLIRSVLDSGGFAVVFDMGDGYKSLCENMGGVYLDGETLRFNPFANITDIDQSAERVRDQLS
VMASPNGNLDEVHEGLLLQAVRASWLAKENRARIDDVVDFLKNASDSEQYAGSPTIRSRLDEMIVLLDQY
TANGTYGQYFNSDEPSLRDDAKMVVLELGGLEDRPSLLVAVMFSLIIYIENRMYRTPRTLKKLNVIDEGW
RLLDFKNRKVGEFIQKGYRTCRRHTGAYITITQNIVDFDSDKASSAARAAWGNSSYKIILKQSAKEFAKY
NQLFPDQFQPLQRDMIGKFGAAKDQWFSSFLLQVENHSSWHRLFVDPLSRAMYSSDGPDFEFVQQKRREG
LSIHEAVWQLAWKKSGPEMASLEAWLEEHEKYRSVA

  Protein domains


Predicted by InterproScan.

(291-447)

(468-764)

(39-277)

  Protein structure



No available structure.




T4SS


T4SS were predicted by using oriTfinder2.

Region 1: 30663..60093

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
JMV05_RS24405 30663..32915 - 2253 WP_128867119 type IV conjugative transfer system coupling protein TraD prgC
JMV05_RS24410 32966..33703 - 738 WP_012881122 hypothetical protein -
JMV05_RS24415 33906..34637 - 732 WP_012881123 conjugal transfer complement resistance protein TraT -
JMV05_RS24420 34651..35160 - 510 WP_000628111 conjugal transfer entry exclusion protein TraS -
JMV05_RS24425 35157..37982 - 2826 WP_033807963 conjugal transfer mating-pair stabilization protein TraG traG
JMV05_RS24430 37979..39352 - 1374 WP_033807962 conjugal transfer pilus assembly protein TraH traH
JMV05_RS24435 39339..39731 - 393 WP_000659962 F-type conjugal transfer protein TrbF -
JMV05_RS24440 39712..40059 - 348 WP_071571857 P-type conjugative transfer protein TrbJ -
JMV05_RS24445 39989..40534 - 546 WP_000059829 type-F conjugative transfer system pilin assembly thiol-disulfide isomerase TrbB traF
JMV05_RS24450 40521..40805 - 285 WP_000624107 type-F conjugative transfer system pilin chaperone TraQ -
JMV05_RS24455 40886..41221 + 336 WP_001620807 hypothetical protein -
JMV05_RS24460 41202..41543 - 342 WP_000556795 conjugal transfer protein TrbA -
JMV05_RS24465 41557..42300 - 744 WP_001030371 type-F conjugative transfer system pilin assembly protein TraF traF
JMV05_RS24470 42293..42550 - 258 WP_033807961 conjugal transfer protein TrbE -
JMV05_RS24475 42577..44481 - 1905 WP_225620370 type-F conjugative transfer system mating-pair stabilization protein TraN traN
JMV05_RS24480 44717..45097 - 381 WP_001462111 hypothetical protein -
JMV05_RS24485 45094..45732 - 639 WP_033807960 type-F conjugative transfer system pilin assembly protein TrbC trbC
JMV05_RS24490 45741..46046 - 306 WP_000224418 hypothetical protein -
JMV05_RS24495 46076..47068 - 993 WP_000830849 conjugal transfer pilus assembly protein TraU traU
JMV05_RS24500 47065..47697 - 633 WP_001203720 type-F conjugative transfer system protein TraW traW
JMV05_RS24505 47694..48080 - 387 WP_000214084 type-F conjugative transfer system protein TrbI -
JMV05_RS24510 48077..50707 - 2631 WP_000069772 type IV secretion system protein TraC virb4
JMV05_RS24515 50833..51180 - 348 WP_000836682 hypothetical protein -
JMV05_RS24520 51208..51426 - 219 WP_033807959 hypothetical protein -
JMV05_RS24525 51525..51998 - 474 WP_033807958 hypothetical protein -
JMV05_RS24530 51991..52404 - 414 WP_000549589 hypothetical protein -
JMV05_RS24535 52397..52618 - 222 WP_001278683 conjugal transfer protein TraR -
JMV05_RS24540 52753..53268 - 516 WP_000809881 type IV conjugative transfer system lipoprotein TraV traV
JMV05_RS24545 53265..53585 - 321 WP_012881128 conjugal transfer protein TrbD -
JMV05_RS24550 53572..54162 - 591 WP_000002784 conjugal transfer pilus-stabilizing protein TraP -
JMV05_RS24555 54152..55579 - 1428 WP_033807957 F-type conjugal transfer pilus assembly protein TraB traB
JMV05_RS24560 55579..56307 - 729 WP_001230769 type-F conjugative transfer system secretin TraK traK
JMV05_RS24565 56294..56860 - 567 WP_000399780 type IV conjugative transfer system protein TraE traE
JMV05_RS24570 56882..57193 - 312 WP_000012113 type IV conjugative transfer system protein TraL traL
JMV05_RS24575 57208..57567 - 360 WP_001098994 type IV conjugative transfer system pilin TraA -
JMV05_RS24580 57601..57828 - 228 WP_000589556 conjugal transfer relaxosome protein TraY -
JMV05_RS24585 57948..58595 - 648 WP_000332523 transcriptional regulator TraJ family protein -
JMV05_RS24590 58787..59170 - 384 WP_001151529 conjugal transfer relaxosome DNA-binding protein TraM -
JMV05_RS24595 59503..60093 + 591 WP_000252683 transglycosylase SLT domain-containing protein virB1
JMV05_RS24600 60390..61211 - 822 WP_001234445 DUF932 domain-containing protein -
JMV05_RS24605 61322..61618 - 297 WP_001272251 hypothetical protein -
JMV05_RS24610 61918..62214 + 297 Protein_78 hypothetical protein -
JMV05_RS24615 62533..62658 - 126 WP_001372321 type I toxin-antitoxin system Hok family toxin -
JMV05_RS24620 62600..62749 - 150 Protein_80 plasmid maintenance protein Mok -
JMV05_RS24625 62771..62950 + 180 WP_001334662 hypothetical protein -
JMV05_RS24630 62928..63690 - 763 Protein_82 plasmid SOS inhibition protein A -
JMV05_RS24635 63687..64121 - 435 WP_000845953 conjugation system SOS inhibitor PsiB -


Host bacterium


ID   18722 GenBank   NZ_LR878367
Plasmid name   83_pKSR100 Incompatibility group   -
Plasmid size   72593 bp Coordinate of oriT [Strand]   59171..59522 [-]
Host baterium   Shigella flexneri 3a isolate 83

Cargo genes


Drug resistance gene   erm(B), mph(A), blaTEM-1B
Virulence gene   -
Metal resistance gene   -
Degradation gene   -
Symbiosis gene   -
Anti-CRISPR   -