Detailed information of oriT

oriT

The information of the oriT region

• oriTDB ID: 118244
• Name: oriT_pBb38S5c
• Organism: Bradyrhizobium barranii subsp. apii strain 38S5
• Sequence Completeness: -
• NCBI accession of oriT (coordinates [strand]): NZ_CP096254 (22899..22954 [+], 56 nt)
• oriT length: 56 nt
• IRs (inverted repeats): 23..29, 34..40 (ACGTCGC..GCGACGT)
• Location of nic site: _
• Conserved sequence flanking the nic site: _
• Note: Predicted by oriTfinder 2.0

oriT sequence

• Length: 56 nt

>oriT_pBb38S5c
CCCCGGCAGGCGGGAAAACAGGACGTCGCGGATGCGACGTATTACTGCGCCCTTGG

oriT secondary structure

Predicted by RNAfold.

Relaxase

traA_J4G48_RS50570_pBb38S5c

• ID: 11595
• GenBank: WP_225049225
• Name: traA_J4G48_RS50570_pBb38S5c
• UniProt ID: _
• Length: 1101 a.a.
• Note: Predicted by oriTfinder 2.0

Relaxase protein sequence

• Length: 1101 a.a.
• Molecular weight: 122094.20 Da
• Isoelectric Point: 9.8900

>WP_225049225.1 Ti-type conjugative transfer relaxase TraA [Bradyrhizobium barranii]
MAITHFTPQLISRGSGRSAVLSAAYRHCARMEHQAEGRTVDYSTKRGLRHEEFLLPPDGPQWARELIADR
SVAGAAEAFWNAIEAFEKRSDAQLAKEFIIALPVELTVDQNIALVREFVATQVLARGQVADWVFHDEPGN
PHIHLMTTLRPLGDNGFGPKKVPVIGDDGAPLRNDFGKIVYRLWSGEKAEFLEQREGWLDLQNKHLALAG
LEIRVDGRSYAERGIDLAPTTHIGVSTKAIDRKGKKAGWSPKLERIALHEERKADNRERMLRRPEIVLDL
VTRELSVFTERDIARVVHRYVDDAGTFQHLMARILQNPDVLRLEGERIALETGIREPAKYTTRELIRLEA
EMAHRAVWLAGRSSHGVREKVLAAAFERHDRLSEEQRVAIAHVAGEGRIAAVVGRAGAGKTTMMKAAREV
WEAAGYRVVGGALAGKASEGLEKEAGIASRTLASWELAWSNGREQLDDKTIFVLDEAGMVSSRQMALFVE
AVVKTGAKLVLVGDPDQLQPIEAGAAFRAIVARTGYAELETIYRQREQWMRDASLDLARGRVAEALQAYG
ANGKLEARKFKAQAVTALIEDWNRDYDPAKSILILAHLRRDVRALNNMARAKLIERGLVEDGQAFCTEDG
QRKFSAGDQIVFLKNEGSLGVKNGMIARVIEARSGWFAAMIGEGERQRRVEVDQRFYANVDHGYATTIHK
SQGATVDRVKVLATLSLDRHLAYVALTRHRENVALYYGAISFGKAGGLTKILSQSRAKETTLDYERGPSY
RAALRFAEARGLHLMRVARTLVADKVRWTVRQSSRLADLGARLLALGARLGLGAARKQTIPNGTKEAKPM
VAGITTFAKSIDQVVEDKLAADPALKKQWEEISTRFRFVFADPEASFRKVDIDSMLKDKATAQSTLSKLA
EKPDSFGALKGKTGLLASRADKQDRQRADLNVPALARDLERFLRLRSEAEHRYEAEERAARLKVAVDIPA
LSPSAKQTLERIRDAIDRNDLPSALEFALEDKMIEAELEGFARAVSERFGERAFVGLSAKDADGKVFEAI
SGGTNAAQKEELKAAWPDLRTAQQLAAQQRTAEAQKEADILRQARRPGLSL

Protein domains

Predicted by InterproScan.

• BID domain of Bartonella effector protein (Bep) (Bep_C_terminal) (858-954)

• MobA/MobL family (MobA_MobL) (17-243)

• AAA domain (AAA_30) (382-566)

• Viral (Superfamily 1) RNA helicase (Viral_helicase1) (692-735)

Protein structure

No available structure.

T4CP

traG_J4G48_RS50585_pBb38S5c

• ID: 13572
• GenBank: WP_248594506
• Name: traG_J4G48_RS50585_pBb38S5c
• UniProt ID: _
• Length: 659 a.a.
• PDB ID: _
• Note: Predicted by oriTfinder 2.0

T4CP protein sequence

• Length: 659 a.a.
• Molecular weight: 70487.61 Da
• Isoelectric Point: 9.8428

>WP_248594506.1 Ti-type conjugative transfer system protein TraG [Bradyrhizobium barranii]
MTANRLLLLIVPMALMLGAIFGLTGIGHGLAGLGKTEAQRLLFARAGLAMPYIAAAGIGVIFLFATAGSA
SIRAAAWSVVAGCVSAISIALAREAMRLSGAPETIVPGRSAVAQIDPATMVAASVVLMGGIFALRVAVKG
NAAFAAPDPKRVRGRRALHGDADWMSMPEAARLFGNAGGIVVGERYRVDRDSAAAQPFRTTNRQSWGFGG
RPPLLCFDASFGSSHGIVFAGSGGFKTTSVTVPTALKWGGALVVLDPSNEVAPMVIAHRRAAGRTVHVLD
PHAGQRTAQGRPPEAAQEATNLPRTGFNALDWIGRFGGTKEEDIAAVASWIMSDSGGARGVRDDFFRASA
LQLLTALIADVCLSGHTEDKNQTLRQVRANLSEPEPKLRARLQAIYDNSESGFVKENVAVFVNMTPETFS
GVYANAVKETHWLSYPNYAALVSGSTFVSDDLAGGQTDVFINLDLKTLETHAGLARVIIGSFMNAIYNRN
GAVTGRALFLLDEVARLGFMRILETARDAGRKYGITLLLLFQSIGQMRETYGGRDAASKWFESASWIAFA
AVNDPETADYISKRCGMTTVEIDQVSRSSQASGSSRTRSKQLASRPLIQPHEVLRMRADEQIVFTAGNAP
LRCGRAIWFRRDDMKACVGHNRFQPEENA

Protein domains

Predicted by InterproScan.

• Type IV secretory system Conjugative DNA transfer (T4SS-DNA_transf) (163-654)

Protein structure

No available structure.

Host bacterium

• ID: 18677
• GenBank: NZ_CP096254
• Plasmid name: pBb38S5c
• Incompatibility group: -
• Plasmid size: 66949 bp
• Coordinate of oriT [Strand]: 22899..22954 [+]
• Host baterium: Bradyrhizobium barranii subsp. apii strain 38S5

Cargo genes

• Drug resistance gene: -
• Virulence gene: -
• Metal resistance gene: -
• Degradation gene: -
• Symbiosis gene: -
• Anti-CRISPR: -