Detailed information of oriT

oriT

The information of the oriT region


oriTDB ID   118238
Name   oriT_pUSA03 in_silico
Organism   Staphylococcus aureus subsp. aureus USA300_FPR3757
Sequence Completeness      -
NCBI accession of oriT (coordinates [strand])   NC_007792 (34223..34258 [+], 36 nt)
oriT length   36 nt
IRs (inverted repeats)      4..11, 13..20  (GCGAACGT..ACGTTCGC)
Location of nic site      29..30
Conserved sequence flanking the
  nic site    		  GTGCGCCCTT
Note   Predicted by oriTfinder 2.0

  oriT sequence  


Download         Length: 36 nt

>oriT_pUSA03
CACGCGAACGTAACGTTCGCATAAGTGCGCCCTTAC

Visualization of oriT structure

  oriT secondary structure

Predicted by RNAfold.

Download structure file


Relaxase

ID   11591 GenBank   WP_011443608
Name   MobA_MobL_SAUSA300_RS16050_pUSA03 insolico UniProt ID   _
Length   303 a.a. PDB ID   
Note   Predicted by oriTfinder 2.0

  Relaxase protein sequence


Download         Length: 303 a.a.        Molecular weight: 35855.17 Da        Isoelectric Point: 9.4972

>WP_011443608.1 MobA/MobL family protein, partial [Staphylococcus aureus]
MAMYHFQNKFVSKANGQSATAKSAYNSASRIKDFKENEFKDYSNKQCDYSEILLPNNADDKFKDREYLWN
KVHDVENRKNSQVAREIIIGLPNEFDPNSNIELAKEFAESLSNEGMIVDLNIHKINEENPHAHLLCTLRG
LDKNNEFEPKRKGNDYIRDWNTKEKHNEWRKRWENVQNKHLEKNGFSVRVSADSYKNQNIDLEPTKKEGW
KARKFEDETGKKSSISKHNESIKKRNQQKIDKMFNEVDDMKSHKLNAFSYMNKSDSTTLKNMAKDLKIYV
TPINMYKENERLYDLKQKNFINH

  Protein domains


Predicted by InterproScan.

MobA/MobL family (MobA_MobL)

(17-216)


  Protein structure



No available structure.




T4CP

ID   13565 GenBank   WP_000209436
Name   t4cp2_SAUSA300_RS14835_pUSA03 insolico UniProt ID   _
Length   546 a.a. PDB ID   _
Note   Predicted by oriTfinder 2.0

  T4CP protein sequence


Download         Length: 546 a.a.        Molecular weight: 62672.60 Da        Isoelectric Point: 7.6569

>WP_000209436.1 MULTISPECIES: VirD4-like conjugal transfer protein, CD1115 family [Bacilli]
MTTLLGELESKATSINKKNLVIQDFDTKCPNRNIFVVGGPGSYKSAGYVIPNVIVKNQQSIVVTDPSGEV
YEKTSNIKRMQGFDVRVVNFKNFLASDRQNFFDYIDKDSDCSIVAELIIKSAGDSKINKDVWYKASVGLL
NSLLLYAKYEFEPEKRTIGNIIKFIQNNKPNQDEEGSVELDKRFNELSKDHPARESYEFGFAVSEGRTRA
SIILTFVADLRNYIDNEIRSYTSDSDFDLRDVGLRKTIIYVMLPVLGNTVQSLSSLFFSQMFQQLYRLGD
ENGARLPVPVDFLLDEWPNIGAIPDYEETLATCRKYGISITTIVQSISQAVDKYNKDKANAIIGNHAVIL
CLGNVNNDTAKYIKEELGNATVEFETSSEGSSSGKDSRSKSSNKNVSYTGRALLNEDEISNMQQDKAILI
TKNRNPKIIKKLAYFKMFPNLTETYIAPQNEYKRKKNQSMIDKHNRLREEWDKKQEKNKQQKLEEYKEEQ
RQKELEKEQQPQEEQQNEKVKESKDKNDQEDKKDEQQKINDSKKAFYEKLKQKQAK

  Protein domains


Predicted by InterproScan.

Type IV secretory system Conjugative DNA transfer (T4SS-DNA_transf)

(3-462)

  Protein structure



No available structure.




T4SS

T4SS were predicted by using oriTfinder2.

Region 1: 11407..22628

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
SAUSA300_RS14765 (SAUSA300_pUSA030006) 6681..7157 + 477 WP_000664727 replication/maintenance protein RepL -
SAUSA300_RS14770 (SAUSA300_pUSA030007) 7865..8599 + 735 WP_001003260 23S rRNA (adenine(2058)-N(6))-methyltransferase Erm(C) -
SAUSA300_RS14775 (SAUSA300_pUSA030008) 8896..9570 + 675 WP_001106019 IS6-like element IS257 family transposase -
SAUSA300_RS14780 (SAUSA300_pUSA030009) 9679..9867 - 189 WP_001063224 hypothetical protein -
SAUSA300_RS14785 (SAUSA300_pUSA030010) 10039..11013 + 975 WP_000368849 conjugative transfer protein TrsA -
SAUSA300_RS14790 (SAUSA300_pUSA030011) 11030..11347 + 318 WP_000591622 CagC family type IV secretion system protein -
SAUSA300_RS14795 (SAUSA300_pUSA030012) 11407..11814 + 408 WP_000110157 hypothetical protein trsC
SAUSA300_RS14800 (SAUSA300_pUSA030013) 11801..12484 + 684 WP_000979860 TrsD/TraD family conjugative transfer protein trsD
SAUSA300_RS14805 (SAUSA300_pUSA030014) 12505..14517 + 2013 WP_000874919 VirB4 family type IV secretion system protein virb4
SAUSA300_RS14810 (SAUSA300_pUSA030015) 14529..15809 + 1281 WP_000702361 membrane protein trsF
SAUSA300_RS14815 (SAUSA300_pUSA030016) 15827..16903 + 1077 WP_000269385 CHAP domain-containing protein trsG
SAUSA300_RS14820 (SAUSA300_pUSA030017) 16913..17398 + 486 WP_000735569 TrsH/TraH family protein -
SAUSA300_RS14825 (SAUSA300_pUSA030018) 17414..19516 + 2103 WP_001094109 DNA topoisomerase III -
SAUSA300_RS14830 (SAUSA300_pUSA030019) 19532..19996 + 465 WP_000715273 hypothetical protein trsJ
SAUSA300_RS14835 (SAUSA300_pUSA030020) 19993..21633 + 1641 WP_000209436 VirD4-like conjugal transfer protein, CD1115 family -
SAUSA300_RS14840 (SAUSA300_pUSA030021) 21711..22628 + 918 WP_000383804 conjugal transfer protein TrbL family protein trsL
SAUSA300_RS14845 (SAUSA300_pUSA030022) 22645..23037 + 393 WP_000608970 single-stranded DNA-binding protein -
SAUSA300_RS15915 (SAUSA300_pUSA030023) 23094..23261 + 168 WP_000869301 hypothetical protein -
SAUSA300_RS14855 (SAUSA300_pUSA030024) 23299..23973 + 675 WP_001105950 IS6-like element IS257 family transposase -
SAUSA300_RS14860 (SAUSA300_pUSA030025) 24103..24717 + 615 WP_000527296 hypothetical protein -
SAUSA300_RS14865 (SAUSA300_pUSA030026) 24786..26057 + 1272 WP_000386736 hypothetical protein -
SAUSA300_RS14870 (SAUSA300_pUSA030027) 26099..26515 - 417 WP_000691761 YolD-like family protein -
SAUSA300_RS14875 (SAUSA300_pUSA030028) 26810..27367 + 558 WP_001273859 recombinase family protein -

Host bacterium

ID   18671 GenBank   NC_007792
Plasmid name   pUSA03 Incompatibility group   -
Plasmid size   37136 bp Coordinate of oriT [Strand]   34223..34258 [+]
Host baterium   Staphylococcus aureus subsp. aureus USA300_FPR3757

Cargo genes

Drug resistance gene   mupA, erm(C)
Virulence gene   -
Metal resistance gene   -
Degradation gene   -
Symbiosis gene   -
Anti-CRISPR   AcrIIA21