Detailed information of oriT

oriT

The information of the oriT region


oriTDB ID   118162
Name   oriT_ECL412|unnamed5 in_silico
Organism   Enterobacter mori strain ECL412
Sequence Completeness      -
NCBI accession of oriT (coordinates [strand])   NZ_JAMYDB010000080 (22705..22855 [+], 151 nt)
oriT length   151 nt
IRs (inverted repeats)      93..98, 106..111  (TGGCCT..AGGCCA)
 12..17, 24..29  (AACCCT..AGGGTT)
Location of nic site      _
Conserved sequence flanking the
  nic site    		  _
Note   Predicted by oriTfinder 2.0

  oriT sequence  


Download         Length: 151 nt

>oriT_ECL412|unnamed5
TGGTCGATGTGAACCCTTTCGACAGGGTTATGAATGAATTGAAAAGTCGTGGCCGCAAGAACGCTCACATCCTGAGCATCCTCCAATTCGACTGGCCTGCATCGGAGGCCATCATCGAGAAGCTGAGCTGCTACATCACAGACGGGATTAA

Visualization of oriT structure

  oriT secondary structure

Predicted by RNAfold.

Download structure file


Relaxase

ID   11538 GenBank   WP_001337757
Name   mobH_NJA11_RS13455_ECL412|unnamed5 insolico UniProt ID   A0A709FRI2
Length   992 a.a. PDB ID   
Note   Predicted by oriTfinder 2.0

  Relaxase protein sequence


Download         Length: 992 a.a.        Molecular weight: 109581.85 Da        Isoelectric Point: 4.8956

>WP_001337757.1 MULTISPECIES: MobH family relaxase [Gammaproteobacteria]
MLKALNKLFGGRSGVIETAPSARVLPLKDVEDEEIPRYPPFAKGLPVAPLDKILATQAELIEKVRNSLGF
TVDDFNRLVLPVIQRYAAFVHLLPASESHHHRGAGGLFRHGLEVAFWAAQASESVIFSIEGTPRERRDNE
PRWRLASCFSGLLHDVGKPLSDVSITDKDGSITWNPYSESLHDWAHRHEIDRYFIRWRDKRHKRHEQFSL
LAVDRIIPAETREFLSKSGPSIMEAMLEAISGTSVNQPVTKLMLRADQESVSRDLRQSRLDVDEFSYGVP
VERYVFDAIRRLVKTGKWKVNEPGAKVWHLNQGVFIAWKQLGDLYDLISHDKIPGIPRDPDTLADILIER
GFAVPNTVQEKGERAYYRYWEVLPEMLQEAAGSVKILMLRLESNDLVFTTEPPAAVAAEVVGDVEDAEIE
FVDPEEADDGDDQEEGEAALNDDMLAAEQEAEKALAGLGFGDAMEMLKSTSDAVEEKPEQKDAGPTESSK
PDAGKKGKPQSKPGKAKPKSDTEKQPHKPEAKEDLSPQDIAKNAPPLANDNPLQALKDVGGGLGDIDFPF
DAFNASAETTSTDATNSEIPDVAMPGKQEEQPKQDFVPQEQNSLQGDDFPMFGGSDEPPSWAIEPLPMLT
DAPEQPTHTPEMPHTDNVNQHEKDAKTLLVEMLSGYGEASALLEQAIMPVLEGKTTLGEVLCLMKGQAVI
LYPDGARSLGAPSEVLSKLSHANAIVPDPIMPGRKVRDFSGVKAIVLAEQLSDAVVAAIKDAEASMGGYQ
DAFELVSPPGLDASKNKSAPKQQSRKKAQQQKPEVNAGKASPEQKAKGKDSQPQPKEKKVDVTSPVEEQQ
RKPVQEKQNVARLPKREAQPVAPEPKVEREKELGHVEVREREDPEVREFEPPKAKTNPKDINAEDFLPSG
VTPQKALQMLKDMIQKRSGRWLVTPVLEEDGCLVTSDKAFDMIAGENIGISKHILCGMLSRAQRRPLLKK
RQGKLYLEVNET

  Protein domains


Predicted by InterproScan.

Putative helicase (TraI_2)

(51-357)


  Protein structure


Source ID Structure
AlphaFold DB A0A709FRI2


T4CP

ID   13490 GenBank   WP_000178856
Name   traD_NJA11_RS13460_ECL412|unnamed5 insolico UniProt ID   _
Length   621 a.a. PDB ID   _
Note   Predicted by oriTfinder 2.0

  T4CP protein sequence


Download         Length: 621 a.a.        Molecular weight: 69197.66 Da        Isoelectric Point: 6.9403

>WP_000178856.1 MULTISPECIES: conjugative transfer system coupling protein TraD [Gammaproteobacteria]
MTMSYDPLAYEMPWRPNYEKNAVAGWLAASGAALAVEQVSTMPPEPFYWMTGICGVMAMARLPKAIKLHL
LQKHLKGRDLEFISIAELQKYIKDTPDDMWLGSGFLWENRHAQRVFEILKRDWTSIVGRESTVKKVVRKI
QGKKKELPIGQPWIHGVEPKEEKLMQPLKHTEGHSLIVGTTGSGKTRMFDILISQAILRGEAVIIIDPKG
DKEMRDNARRACEAMGQPERFVSFHPAFPEESVRIDPLRNFTRVTEIASRLAALIPSEAGADPFKSFGWQ
ALNNIAQGLVITHDRPNLTKLRRFLEGGAAGLVIKAVQAYSERVMPDWEAEAAAYLEKAKNGSREKIAFA
LMKFYYDIIQPEHPNSDLEGLLSMFQHDQTHFSKMVANLLPIMNMLTSGELGPLLSPDSSDLSDERQITD
SAKIINNAQVAYLGLDSLTDNMVGSAMGSIFLSDLTAVAGDRYNYGVNNRPVNIFVDEAAEVINDPFIQL
LNKGRGAKLRLFVATQTFADFAARLGSKDKALQVLGNINNTFALRIVDGETQEYIADNLPKTRLKYVMRT
QGQNSDGKEPIMHGGNQGERLMEEEADLFPAQLLGMLPNLEYIAKISGGTIVKGRLPILTQ

  Protein domains


Predicted by InterproScan.

TraM recognition site of TraD and TraG (TraG-D_C)

(471-598)

Type IV secretion-system coupling protein DNA-binding domain (TrwB_AAD_bind)

(170-297)

  Protein structure



No available structure.



ID   13491 GenBank   WP_000637386
Name   traC_NJA11_RS13535_ECL412|unnamed5 insolico UniProt ID   _
Length   815 a.a. PDB ID   _
Note   Predicted by oriTfinder 2.0

  T4CP protein sequence


Download         Length: 815 a.a.        Molecular weight: 92590.87 Da        Isoelectric Point: 5.9222

>WP_000637386.1 MULTISPECIES: type IV secretion system protein TraC [Gammaproteobacteria]
MIVTIKKKLEETLIPEHLRAAGIIPVLAYDEDDHVFLMDDHSAGFGFMCEPLCGADEKVQERMNGFLNQE
FPSKTTLQFVLFRSPDINQEMYRMMGLRDGFRHELLTSVIKERINFLQHHTTERIFAKTNKGIYDNGLIQ
DLKLFVTCKVPIKNNNPTESELQQLAQLRTKVESSLQTVGLRPRTMTAVNYIRIMSTILNWGPDASWRHD
SVDWEMDKPICEQIFDYGTDVEVSKNGIRLGDYHAKVMSAKKLPDVFYFGDALTYAGDLSGGNSSIKENY
MVVTNVFFPEAESTKNTLERKRQFTVNQAYGPMLKFVPVLADKKESFDTLYESMKEGAKPVKITYSVVLF
APTKERVEAAAMAARNIWRESRFELMEDKFVALPMFLNCLPFCTDRDAVRDLFRYKTMTTEQAAVVLPVF
GEWKGTGTYHAALISRNGQLMSLSLHDSNTNKNLVIAAESGSGKSFLTNELIFSYLSEGAQVWVIDAGKS
YQKLSEMLNGDFVHFEEGTHVCLNPFELIQNYEDEEDAIVSLVCAMASAKGLLDEWQISALKQVLSRLWE
EKGKEMKVDDIAERCLEEENDQRLKDIGQQLYAFTSKGSYGKYFSRKNNVSFQNQFTVLELDELQGRKHL
RQVVLLQLIYQIQQEVFLGERNRKKVVIVDEAWDLLKEGEVSVFMEHAYRKFRKYGGSVVIATQSINDLY
ENAVGRAIAENSASMYLLGQTEETVESVKRSGRLTLSEGGFHTLKTVHTIQGVYSEIFIKSKSGMGVGRL
IVGDFQKLLYSTDPVDVNAIDQFVKQGMSIPEAIKAVMRSRQQAA

  Protein domains


Predicted by InterproScan.

TraG P-loop domain (P-loop_TraG)

(449-809)

Family of unknown function (DUF5934) (DUF5934)

(284-434)

TraC protein (TraC_F_IV)

(21-255)

  Protein structure



No available structure.




T4SS

T4SS were predicted by using oriTfinder2.

Region 1: 20789..45980

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
NJA11_RS13445 (NJA11_13445) 16814..17047 - 234 WP_001191892 hypothetical protein -
NJA11_RS13450 (NJA11_13450) 17029..17646 - 618 WP_001249396 hypothetical protein -
NJA11_RS13455 (NJA11_13455) 17814..20792 + 2979 WP_001337757 MobH family relaxase -
NJA11_RS13460 (NJA11_13460) 20789..22654 + 1866 WP_000178856 conjugative transfer system coupling protein TraD virb4
NJA11_RS13465 (NJA11_13465) 22704..23249 + 546 WP_000228720 hypothetical protein -
NJA11_RS13470 (NJA11_13470) 23206..23835 + 630 WP_000743450 DUF4400 domain-containing protein tfc7
NJA11_RS13475 (NJA11_13475) 23845..24291 + 447 WP_000122506 hypothetical protein -
NJA11_RS13480 (NJA11_13480) 24301..24678 + 378 WP_000869261 hypothetical protein -
NJA11_RS13485 (NJA11_13485) 24678..25340 + 663 WP_001231463 hypothetical protein -
NJA11_RS13490 (NJA11_13490) 25676..26041 + 366 WP_001052531 hypothetical protein -
NJA11_RS13495 (NJA11_13495) 26186..26467 + 282 WP_000805625 type IV conjugative transfer system protein TraL traL
NJA11_RS13500 (NJA11_13500) 26464..27090 + 627 WP_001049716 TraE/TraK family type IV conjugative transfer system protein traE
NJA11_RS13505 (NJA11_13505) 27074..27991 + 918 WP_000794249 type-F conjugative transfer system secretin TraK traK
NJA11_RS13510 (NJA11_13510) 27991..29304 + 1314 WP_024131605 TraB/VirB10 family protein traB
NJA11_RS13515 (NJA11_13515) 29301..29879 + 579 WP_000793435 type IV conjugative transfer system lipoprotein TraV traV
NJA11_RS13520 (NJA11_13520) 29883..30275 + 393 WP_000479535 TraA family conjugative transfer protein -
NJA11_RS13525 (NJA11_13525) 30476..36007 + 5532 WP_000606833 Ig-like domain-containing protein -
NJA11_RS13530 (NJA11_13530) 36156..36863 + 708 WP_001259347 DsbC family protein -
NJA11_RS13535 (NJA11_13535) 36860..39307 + 2448 WP_000637386 type IV secretion system protein TraC virb4
NJA11_RS13540 (NJA11_13540) 39322..39639 + 318 WP_000351984 hypothetical protein -
NJA11_RS13545 (NJA11_13545) 39636..40166 + 531 WP_001010738 S26 family signal peptidase -
NJA11_RS13550 (NJA11_13550) 40129..41394 + 1266 WP_000621288 TrbC family F-type conjugative pilus assembly protein traW
NJA11_RS13555 (NJA11_13555) 41391..42062 + 672 WP_001337754 EAL domain-containing protein -
NJA11_RS13560 (NJA11_13560) 42062..43066 + 1005 WP_043940352 TraU family protein traU
NJA11_RS13565 (NJA11_13565) 43182..45980 + 2799 WP_001256487 conjugal transfer mating pair stabilization protein TraN traN
NJA11_RS13570 (NJA11_13570) 46019..46879 - 861 WP_000709517 hypothetical protein -
NJA11_RS13575 (NJA11_13575) 47002..47643 - 642 WP_000796664 hypothetical protein -
NJA11_RS13580 (NJA11_13580) 47937..48257 + 321 WP_000547566 hypothetical protein -
NJA11_RS13585 (NJA11_13585) 48561..48746 + 186 WP_001186917 hypothetical protein -
NJA11_RS13590 (NJA11_13590) 48966..49934 + 969 WP_000085162 AAA family ATPase -
NJA11_RS13595 (NJA11_13595) 49945..50853 + 909 WP_000739139 hypothetical protein -

Host bacterium

ID   18595 GenBank   NZ_JAMYDB010000080
Plasmid name   ECL412|unnamed5 Incompatibility group   IncA/C2
Plasmid size   122265 bp Coordinate of oriT [Strand]   22705..22855 [+]
Host baterium   Enterobacter mori strain ECL412

Cargo genes

Drug resistance gene   aph(6)-Id, aph(3'')-Ib, sul2
Virulence gene   -
Metal resistance gene   -
Degradation gene   -
Symbiosis gene   -
Anti-CRISPR   -