Detailed information of oriT

oriT

The information of the oriT region

• oriTDB ID: 118094
• Name: oriT_PCH555|unnamed1
• Organism: Enterococcus faecalis strain PCH555
• Sequence Completeness: -
• NCBI accession of oriT (coordinates [strand]): NZ_CP090559 (4138..4187 [-], 50 nt)
• oriT length: 50 nt
• IRs (inverted repeats): _
• Location of nic site: _
• Conserved sequence flanking the nic site: _
• Note: Predicted by oriTfinder 2.0

oriT sequence

• Length: 50 nt

>oriT_PCH555|unnamed1
AATATCGCAACATGCTAGCATGTTGCTCTGCTCGCAAAAAGAAAGCCTAC

oriT secondary structure

Predicted by RNAfold.

Relaxase

Relaxase_LZP99_RS13460_PCH555|unnamed1

• ID: 11477
• GenBank: WP_010784125
• Name: Relaxase_LZP99_RS13460_PCH555|unnamed1
• UniProt ID: _
• Length: 561 a.a.
• Note: Predicted by oriTfinder 2.0

Relaxase protein sequence

• Length: 561 a.a.
• Molecular weight: 65427.18 Da
• Isoelectric Point: 6.1241

>WP_010784125.1 relaxase/mobilization nuclease domain-containing protein [Enterococcus faecalis]
MVYTKHFVIHTFDKLNNACSYIENAEKTEVTNDNPSEHLEHLFQYIVNDDKTYMKKLVSGHGIVDPTNPY
EEFKLTKLQAAIQRKIGYTFDPKSERLLPPTLTELEKGNAVLAHHLIQSFSPEDDLTPEKTHEIGYNTVM
ELTGGEYEFVIATHVDKEHLHNHIIFSSTNLKTGKAFRWQKGTKRVFEQISDKIAAKEGAKIIEKSPKNS
HKKYTMWETESLYKEKIKSRLDFLLEQSSSINDFLEKAEALNLSVDFSKKWTTYRLLDEPQIKNTRSRSL
SKNDPTRYNYEKIVERLKENKSVLTLKEVVEKYVEKGEKSKNEFDYQLTIDEWQISHKTTRGYYVNVDFG
FGERGKVFIGAYKVEPLENGQYNIYVKRKDFFYLMNEKNADRNRYMTGETLIKQLRLYNGQTPLKKEPVM
RTIDELVNAINFLAANEIEDTRQLELLEEKLEAAFLEAEETLETLDEKMLELHQLSNLLLENELNGELEL
IQKKLKTLLPDATLAEFSYEDVRGEIEAIKTSQSLLENKLERTRNEINQLHEIQAVQKKETANQNNVKPK
L

Protein domains

Predicted by InterproScan.

• Relaxase/Mobilisation nuclease domain (Relaxase) (37-296)

Protein structure

No available structure.

Auxiliary protein

WP_010784124_PCH555|unnamed1

• ID: 6595
• GenBank: WP_010784124
• Name: WP_010784124_PCH555|unnamed1
• UniProt ID: _
• Length: 118 a.a.
• PDB ID: _
• Note: Predicted by oriTfinder 2.0

Auxiliary protein sequence

• Length: 118 a.a.
• Molecular weight: 13987.11 Da
• Isoelectric Point: 10.1993

>WP_010784124.1 MULTISPECIES: plasmid mobilization relaxosome protein MobC [Enterococcus]
MENKQKLKRPIQRIVRLSEEENNLIKRKIEKSFFPNFQNFALHLLIQGEIRHVDYSELNRLTTEIHKIGI
NINQMARLANQFHEISSEDIKDLTDKVHSLNALVQSELNKLIKRKEQS

Protein domains

Predicted by InterproScan.

• Bacterial mobilisation protein (MobC) (MobC) (64-105)

Protein structure

No available structure.

T4CP

t4cp2_LZP99_RS13480_PCH555|unnamed1

• ID: 13413
• GenBank: WP_048948780
• Name: t4cp2_LZP99_RS13480_PCH555|unnamed1
• UniProt ID: _
• Length: 609 a.a.
• PDB ID: _
• Note: Predicted by oriTfinder 2.0

T4CP protein sequence

• Length: 609 a.a.
• Molecular weight: 70085.17 Da
• Isoelectric Point: 6.4057

>WP_048948780.1 MULTISPECIES: VirD4-like conjugal transfer protein, CD1115 family [Enterococcus]
MQTYKKELKPYLYLGIFLFVIGFGIGNFFWLLPGTDYFSKTNYLFTKDILTYIEQTFYRFFITPSLLGFS
VGILGFLLGLLMYVRDNDRGIYRHGEEYGSARFATPAEMKKYEDPIPENNIIVSKHVKISLFNKRLPIKL
QKNKNIAILGDSGAAKTLAFIKTNLMQRHASFITTDPDGGILPEIGLLLKKGKYKIKVLDLNTLSNSDTF
NVFEYMHSELDVDRVLEAVTEATKEVEKTTGDDFWIKAEGLLVRSLIAYLWFDGRRNDYTPHLGMIADML
RHLKRKDKNVPSPVEEWFEELNVAIPDNYAYRQWTLFNNLYESETRASVLGIAAARYSVFDHEEVVNMIR
TDTMDIDSWNEVKTAVFIAIPETNTSFNFIAALMFATVGEVLRNKSDQVRLGKRALEEGKELLHVRFLID
EFANIGRIPHFEKMLTTFRKREMSFTIVLQSLNQLQTLYRNGWQNILNGCACLLYLGGDEEETTKYLSAR
AGKQTLSIRKHSMNKGNQGGGSENRDKYGRDLIDRSEVALIGGDECLVFISKEHVFKDKKFYAFQHDRAD
ELATGPTDDKWYTWRRYMTDEEAILDKISATDIIDHGEITSELKEETHV

Protein domains

Predicted by InterproScan.

• Type IV secretory system Conjugative DNA transfer (T4SS-DNA_transf) (144-558)

Protein structure

No available structure.

Host bacterium

• ID: 18527
• GenBank: NZ_CP090559
• Plasmid name: PCH555|unnamed1
• Incompatibility group: -
• Plasmid size: 77536 bp
• Coordinate of oriT [Strand]: 4138..4187 [-]
• Host baterium: Enterococcus faecalis strain PCH555

Cargo genes

• Drug resistance gene: ClpL
• Virulence gene: -
• Metal resistance gene: -
• Degradation gene: -
• Symbiosis gene: -
• Anti-CRISPR: AcrIIA21, AcrIIA9