Detailed information of oriT
oriT
The information of the oriT region
| oriTDB ID | 118065 |
| Name | oriT_29212|1  |
| Organism | Enterococcus faecalis ATCC 29212 |
| Sequence Completeness | - |
| NCBI accession of oriT (coordinates [strand]) | NZ_CP008815 (47573..47622 [+], 50 nt) |
| oriT length | 50 nt |
| IRs (inverted repeats) | _ |
| Location of nic site | 34..35 |
| Conserved sequence flanking the nic site |
GCTTGCAAAA |
| Note | Predicted by oriTfinder 2.0 |
oriT sequence
Download Length: 50 nt
>oriT_29212|1
AATATCGCAACATGGTACCATGTTGCTCCGCTTGCAAAAAGAAAGCCTAC
AATATCGCAACATGGTACCATGTTGCTCCGCTTGCAAAAAGAAAGCCTAC
Visualization of oriT structure
oriT secondary structure
Predicted by RNAfold.
Download structure file
Relaxase
| ID | 11465 | GenBank | WP_010784826 |
| Name | Relaxase_DR75_RS00525_29212|1 |
UniProt ID | A0A8E7CAC2 |
| Length | 561 a.a. | PDB ID | |
| Note | Predicted by oriTfinder 2.0 | ||
Relaxase protein sequence
Download Length: 561 a.a. Molecular weight: 65352.94 Da Isoelectric Point: 6.0135
>WP_010784826.1 MULTISPECIES: relaxase/mobilization nuclease domain-containing protein [Enterococcus]
MVYTKHFVIHTFDKLNNACSYIENAEKTEVTNDNPSEHLEHLFQYIVNDDKTYMKKLVSGHGIVDPTNPY
EEFKLTKLQAAIQRKIGYTFDPKSERLLPPTLTELEKGNAVLAHHLIQSFSPEDDLTPEKIHEIGYNTVM
ELTGGEYEFVIATHVDKEHLHNHIIFSSTNLKTGKAFRWQKGTKKVFEQISDKIAAKEGAKIIEKSPKQT
HTKYTKWQTESIYKQKIKSRLDFLLEQSSSINDFLEKAEALNLSADFSNKWTTYRLLDEPQIKNTRSRSL
SKSDPTRYNYEKIIERLKENKNVLTVEECVKRYEEKNEQEKNNFDYQFTIEPWQLSHKTERGYYINIDYG
YGNSGKLFVGGYKVDPLDNGNYNIYIKRNEYFYFMSDKDATKSKYLTGASLIKQLRLYNGQTPLKREPVM
QTIDELVSAINFLAANEIEDTRQLKLLEEKLEVAFLEAEKTLETLDEKMLELHQLSNLLLENELQGDPEL
IQKKLKTLLPDATLAEFSYEDVRGEIEAIKTSQTLLESKLERTRNEINQLHEIQAVQEKEPEKQTDIKPK
L
MVYTKHFVIHTFDKLNNACSYIENAEKTEVTNDNPSEHLEHLFQYIVNDDKTYMKKLVSGHGIVDPTNPY
EEFKLTKLQAAIQRKIGYTFDPKSERLLPPTLTELEKGNAVLAHHLIQSFSPEDDLTPEKIHEIGYNTVM
ELTGGEYEFVIATHVDKEHLHNHIIFSSTNLKTGKAFRWQKGTKKVFEQISDKIAAKEGAKIIEKSPKQT
HTKYTKWQTESIYKQKIKSRLDFLLEQSSSINDFLEKAEALNLSADFSNKWTTYRLLDEPQIKNTRSRSL
SKSDPTRYNYEKIIERLKENKNVLTVEECVKRYEEKNEQEKNNFDYQFTIEPWQLSHKTERGYYINIDYG
YGNSGKLFVGGYKVDPLDNGNYNIYIKRNEYFYFMSDKDATKSKYLTGASLIKQLRLYNGQTPLKREPVM
QTIDELVSAINFLAANEIEDTRQLKLLEEKLEVAFLEAEKTLETLDEKMLELHQLSNLLLENELQGDPEL
IQKKLKTLLPDATLAEFSYEDVRGEIEAIKTSQTLLESKLERTRNEINQLHEIQAVQEKEPEKQTDIKPK
L
Protein domains
Predicted by InterproScan.
Protein structure
No available structure.
Auxiliary protein
| ID | 6587 | GenBank | WP_002367696 |
| Name | WP_002367696_29212|1 |
UniProt ID | Q82YL4 |
| Length | 118 a.a. | PDB ID | _ |
| Note | Predicted by oriTfinder 2.0 | ||
Auxiliary protein sequence
Download Length: 118 a.a. Molecular weight: 14042.10 Da Isoelectric Point: 9.9724
>WP_002367696.1 MULTISPECIES: plasmid mobilization relaxosome protein MobC [Bacteria]
MENKQKLKRPIQRKIRFSKEENELINRKIEESFFPNFQNFALHLLIQGEIRHVDYSELNRLTTEIHKIGI
NINQMARLANQFHEISSEDIKDLTDKVQSLNALVQSELNKLIKRKEQS
MENKQKLKRPIQRKIRFSKEENELINRKIEESFFPNFQNFALHLLIQGEIRHVDYSELNRLTTEIHKIGI
NINQMARLANQFHEISSEDIKDLTDKVQSLNALVQSELNKLIKRKEQS
Protein domains
Predicted by InterproScan.
Protein structure
| Source | ID | Structure |
|---|---|---|
| AlphaFold DB | Q82YL4 |
T4CP
| ID | 13398 | GenBank | WP_002414681 |
| Name | t4cp2_DR75_RS00505_29212|1 |
UniProt ID | _ |
| Length | 609 a.a. | PDB ID | _ |
| Note | Predicted by oriTfinder 2.0 | ||
T4CP protein sequence
Download Length: 609 a.a. Molecular weight: 70072.08 Da Isoelectric Point: 6.1807
>WP_002414681.1 VirD4-like conjugal transfer protein, CD1115 family [Enterococcus faecalis]
MQTYKKELKPYLYLGVFLFVIGFGIGNFFWLLPGTDYFSKTNYLFTKDILTYIEQTFYRFFITPSLLGFS
VGVLGFLLGLLMYVRDNDRGIYRHGEEYGSARFATPAEMKKYEDPIPENNIIVSKHVKISLFNKRLPIKL
QKNKNIAILGDSGAAKTLAFIKTNLMQRHASFITTDPDGGILPEIGLLLKKGKYKIKVLDLNTLSNSDTF
NVFEYMHSELDVDRVLEAVTEATKEVEKTTGDDFWIKAEGLLVRSLIAYLWFDGRRNDYTPHLGMIADML
RHLKRKDKNVPSPAEEWFEELNEAIPDNYAYRQWTLFNNLYESETRASVLGIAAARYSVFDHEEVVNMIR
TDTMDIDSWNEEKTAVFIAIPETNTSFNFIAALMFATVGEVLRNKSDQVRLGKRVLEEGKELLHVRFLID
EFANIGRIPHFEKMLTTFRKREMSFTIVLQSLNQLQTLYPKGWQNILNGCACLLYLGGDEEETTKYLSAR
AGKQTLSIRKHSMNKGNQGGGSENRDKYGRDLIDRSEVALIGGDECLVFISKEHVFKDKKFYAFQHDRAD
ELATGPTDDKWYTWRRYMTDEEAILDKISATDIIDHGEITSELKEETHV
MQTYKKELKPYLYLGVFLFVIGFGIGNFFWLLPGTDYFSKTNYLFTKDILTYIEQTFYRFFITPSLLGFS
VGVLGFLLGLLMYVRDNDRGIYRHGEEYGSARFATPAEMKKYEDPIPENNIIVSKHVKISLFNKRLPIKL
QKNKNIAILGDSGAAKTLAFIKTNLMQRHASFITTDPDGGILPEIGLLLKKGKYKIKVLDLNTLSNSDTF
NVFEYMHSELDVDRVLEAVTEATKEVEKTTGDDFWIKAEGLLVRSLIAYLWFDGRRNDYTPHLGMIADML
RHLKRKDKNVPSPAEEWFEELNEAIPDNYAYRQWTLFNNLYESETRASVLGIAAARYSVFDHEEVVNMIR
TDTMDIDSWNEEKTAVFIAIPETNTSFNFIAALMFATVGEVLRNKSDQVRLGKRVLEEGKELLHVRFLID
EFANIGRIPHFEKMLTTFRKREMSFTIVLQSLNQLQTLYPKGWQNILNGCACLLYLGGDEEETTKYLSAR
AGKQTLSIRKHSMNKGNQGGGSENRDKYGRDLIDRSEVALIGGDECLVFISKEHVFKDKKFYAFQHDRAD
ELATGPTDDKWYTWRRYMTDEEAILDKISATDIIDHGEITSELKEETHV
Protein domains
Predicted by InterproScan.
Protein structure
No available structure.
T4SS
T4SS were predicted by using oriTfinder2.
Region 1: 30553..44928
| Locus tag | Coordinates | Strand | Size (bp) | Protein ID | Product | Description |
|---|---|---|---|---|---|---|
| DR75_RS16520 | 26753..27691 | + | 939 | WP_265183031 | transposase | - |
| DR75_RS00415 (DR75_2916) | 27882..30107 | + | 2226 | Protein_34 | LPXTG-anchored aggregation substance Asa1 | - |
| DR75_RS00420 (DR75_2917) | 30169..30525 | + | 357 | WP_002414676 | pheromone response system RNA-binding regulator PrgU | - |
| DR75_RS00425 (DR75_2918) | 30553..31410 | + | 858 | WP_002414677 | pheromone response LPXTG-anchored adhesin PrgC | prgC |
| DR75_RS00430 (DR75_2919) | 31452..32350 | + | 899 | Protein_37 | type III secretion system protein PrgD | - |
| DR75_RS00435 (DR75_2921) | 32370..32804 | + | 435 | WP_002365857 | hypothetical protein | - |
| DR75_RS00440 (DR75_2922) | 32851..33078 | + | 228 | WP_002365858 | hypothetical protein | - |
| DR75_RS00445 (DR75_2923) | 33091..33387 | + | 297 | WP_002405100 | hypothetical protein | - |
| DR75_RS00450 (DR75_2924) | 33402..34202 | + | 801 | WP_002414678 | type IV secretion system protein | prgHb |
| DR75_RS00455 (DR75_2925) | 34204..34557 | + | 354 | WP_002367919 | PrgI family mobile element protein | prgIc |
| DR75_RS00460 (DR75_2926) | 34511..36901 | + | 2391 | WP_002365863 | VirB4-like conjugal transfer ATPase, CD1110 family | virb4 |
| DR75_RS00465 (DR75_2928) | 36913..39527 | + | 2615 | Protein_44 | phage tail tip lysozyme | - |
| DR75_RS00470 (DR75_2929) | 39551..40177 | + | 627 | WP_002414679 | hypothetical protein | prgL |
| DR75_RS00475 (DR75_2930) | 40179..40409 | + | 231 | WP_227660684 | type III secretion system protein PrgM | - |
| DR75_RS00480 (DR75_2931) | 40393..41001 | + | 609 | WP_002367686 | hypothetical protein | - |
| DR75_RS00485 (DR75_2932) | 41114..41869 | + | 756 | WP_002367687 | Fic family protein | - |
| DR75_RS00490 (DR75_2933) | 41886..42344 | + | 459 | WP_002414680 | single-stranded DNA-binding protein | - |
| DR75_RS00495 (DR75_2934) | 42390..42572 | + | 183 | WP_002367689 | hypothetical protein | - |
| DR75_RS00500 (DR75_2935) | 42614..43099 | + | 486 | WP_010784824 | PcfB family protein | gbs1365 |
| DR75_RS00505 (DR75_2936) | 43099..44928 | + | 1830 | WP_002414681 | VirD4-like conjugal transfer protein, CD1115 family | virb4 |
| DR75_RS00510 (DR75_2937) | 44979..47138 | + | 2160 | WP_010784825 | ArdC-like ssDNA-binding domain-containing protein | - |
| DR75_RS00515 (DR75_2938) | 47171..47443 | + | 273 | WP_002395044 | LtrD | - |
| DR75_RS00520 (DR75_2939) | 47689..48045 | + | 357 | WP_002367696 | plasmid mobilization relaxosome protein MobC | - |
| DR75_RS00525 (DR75_2940) | 48046..49731 | + | 1686 | WP_010784826 | relaxase/mobilization nuclease domain-containing protein | - |
Host bacterium
| ID | 18498 | GenBank | NZ_CP008815 |
| Plasmid name | 29212|1 | Incompatibility group | - |
| Plasmid size | 66548 bp | Coordinate of oriT [Strand] | 47573..47622 [+] |
| Host baterium | Enterococcus faecalis ATCC 29212 |
Cargo genes
| Drug resistance gene | - |
| Virulence gene | cylR2, cylL, cylS, cylM, cylB, cylA, cylI |
| Metal resistance gene | - |
| Degradation gene | - |
| Symbiosis gene | - |
| Anti-CRISPR | AcrIIA9 |