Detailed information of oriT

oriT

The information of the oriT region


oriTDB ID   118026
Name   oriT_pIncLM-1301491 in_silico
Organism   Enterobacter hormaechei strain 2013_1a
Sequence Completeness      -
NCBI accession of oriT (coordinates [strand])   NZ_CP031566 (6299..6403 [-], 105 nt)
oriT length   105 nt
IRs (inverted repeats)      32..37, 40..45  (GCCGGC..GCCGGC)
Location of nic site      _
Conserved sequence flanking the
  nic site    		  _
Note   Predicted by oriTfinder 2.0

  oriT sequence  


Download         Length: 105 nt

>oriT_pIncLM-1301491
AGTACGGGACAAGATGTGTTTTTGGTACACCGCCGGCACGCCGGCAGTGACGCAAAAACGTCTTGGTCAGGGCAAGCCCCGACACCCCCTAACGAGGTTAGCTAT

Visualization of oriT structure

  oriT secondary structure

Predicted by RNAfold.

Download structure file


Relaxase

ID   11431 GenBank   WP_004206924
Name   traI_AXA59_RS23110_pIncLM-1301491 insolico UniProt ID   A0A2V4FJ40
Length   659 a.a. PDB ID   
Note   Predicted by oriTfinder 2.0

  Relaxase protein sequence


Download         Length: 659 a.a.        Molecular weight: 75087.84 Da        Isoelectric Point: 10.0285

>WP_004206924.1 MULTISPECIES: TraI/MobA(P) family conjugative relaxase [Enterobacterales]
MVIPKIIEGRRDKKSSFGQLIKYMADKPSQELTDTVQPTPETALAVKSDMFEGLNNYLTRKQKVISTPVD
VEPGVQRVVVGDVTCQYNTFSLDGAAQEMNSVSQQSTRCKDPVMHYVLSWPDYEKPNDDQVFDSVKFTLA
SMGMSDHQYVAAIHRDTDNLHVHVAVNRINPQTYKAASSSFTKDTLHQACRLLELKNGWSHSNGAYVVND
RQQIVRNPHSKKERGNWRSLDRINKMENKEGVETLYRYIVGDEQVGGSRQNLIHVSAGLREAKSWDDVHK
TFAGIGLRVEKAQGKKGYVITHEHQNQKTAVKASLVFNKAQYTLKSMEERFGEYQPSHIEPAKVSVFKTA
YTPGAYRRDANKRLQRKIERAEERMLLKGRYRAYRNNLPIYSPDKDRIADEYRKIAQHTRLVKNNVRHSV
SDPHTRKLMYNLAEFKRLQAVANLRLSLREERNGFRAANPRLSYREWVEQEALKGDKAALSQMRGFAYSS
RKKEKYKQQLVEQIGFNRTFNAITSHDRDDVAVMASARHGVKPRLLKDGTVIFERDGKPVAADRGHIVLT
ESNGIDKEKTADLAIALTIAGKAKSVRVDGDGEFKELCCNRIVDAAVNHNHPVAQGITFTDAAQQAYAQN
EKHRLIREQNNSKNEMQLRSESDDKFNPK

  Protein domains


Predicted by InterproScan.

Relaxase/Mobilisation nuclease domain (Relaxase)

(86-334)


  Protein structure


Source ID Structure
AlphaFold DB A0A2V4FJ40


T4CP

ID   13361 GenBank   WP_004206885
Name   t4cp2_AXA59_RS23360_pIncLM-1301491 insolico UniProt ID   _
Length   695 a.a. PDB ID   _
Note   Predicted by oriTfinder 2.0

  T4CP protein sequence


Download         Length: 695 a.a.        Molecular weight: 79538.91 Da        Isoelectric Point: 4.8317

>WP_004206885.1 MULTISPECIES: conjugal transfer protein TrbC [Enterobacterales]
MQQESVDSKKVLRPDGSFMEWFLTPNVQFGLLAILTVLGLFLPFTMLLSILILPPLMVAFTDRKFRPPLR
MPKDCEMFDDTLTTETQAEYRLGPIRIPHKVRERKKAKGILYVGYERGRLFGRELWLNMTDLLRHMVFFG
TTGSGKTETFYGFIVNFLLWCRGYCLSDGKADNKLAFATWSLARRFGREDDYYVLNLLTGSIDRFVNLVK
QESIPAQSNSVNLFSVAPPTFIIQLMESMLPQVGGDSAQWQDTAKAMMSALINALCYKRARGELLLSQRT
IQKNMSLPAMAALYVEAKKNGWHSEGYAALESYLENTPGFLLANAEYPETWEARAFEQHNYMSRQFLKTL
SLFNETYGHVFPEDSGDIRMDDIFHNDRILIVMIPSLELSRGEAATLGRLYVTLQRMTISKDLGYQLEGK
KEEVLLTHALNNQAPYGLIYDELGQYFTSGMDTLSAQMRSLEKMGVFSSQDHPSLARGANGEVDSLIANT
RVKYFESIEDRKTFEILRETVGQDYYSELSGQEAEHGTFSKTVREGDLYQIREKDRVNIRELRKQTEGQG
VISFQDALVRSAAFYIPDNEKFSSELPMRINRFIEVLPPSPATLYSIYPERKDDELAETNPDAMRFPPIK
LFGYDKAANSLLEKIEVFYELQCGAISPEEMSVCLFELFAEWLDGTETDDVLYHQEDDLFPMIEM

  Protein domains



No domain identified.


  Protein structure



No available structure.




T4SS

T4SS were predicted by using oriTfinder2.

Region 1: 49968..63664

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
AXA59_RS23405 (AXA59_23645) 45952..46485 + 534 Protein_65 IS110-like element IS4321 family transposase -
AXA59_RS23410 (AXA59_23650) 47657..48712 - 1056 WP_015062846 plasmid replication initiator RepA -
AXA59_RS23415 (AXA59_23655) 49009..49239 - 231 WP_004187496 IncL/M type plasmid replication protein RepC -
AXA59_RS23420 (AXA59_23660) 49453..49965 - 513 WP_235027230 ExcA -
AXA59_RS23425 (AXA59_23665) 49968..52148 - 2181 WP_004187492 DotA/TraY family protein traY
AXA59_RS23430 (AXA59_23670) 52141..52791 - 651 WP_004187488 hypothetical protein -
AXA59_RS23435 (AXA59_23675) 52788..53996 - 1209 WP_004187486 conjugal transfer protein TraW traW
AXA59_RS23440 (AXA59_23680) 53993..57043 - 3051 WP_011154474 conjugative transfer protein traU
AXA59_RS23445 (AXA59_23685) 57040..57534 - 495 WP_004187480 hypothetical protein -
AXA59_RS23450 (AXA59_23690) 57580..57969 - 390 WP_011154472 DUF6750 family protein traR
AXA59_RS23455 (AXA59_23695) 57986..58516 - 531 WP_004187478 conjugal transfer protein TraQ traQ
AXA59_RS23460 (AXA59_23700) 58540..59244 - 705 WP_004206933 conjugal transfer protein TraP traP
AXA59_RS23465 (AXA59_23705) 59256..60605 - 1350 WP_004206932 conjugal transfer protein TraO traO
AXA59_RS23470 (AXA59_23710) 60617..61768 - 1152 WP_015062843 DotH/IcmK family type IV secretion protein traN
AXA59_RS27500 61777..62232 - 456 WP_235027231 DotI/IcmL family type IV secretion protein traM
AXA59_RS27505 62229..62489 - 261 WP_228716118 hypothetical protein traM
AXA59_RS23480 (AXA59_23720) 62557..62967 + 411 WP_004187465 H-NS family nucleoid-associated regulatory protein -
AXA59_RS23485 (AXA59_23725) 62954..63151 + 198 WP_004187464 Hha/YmoA family nucleoid-associated regulatory protein -
AXA59_RS23490 (AXA59_23730) 63152..63664 - 513 WP_011091071 hypothetical protein traL
AXA59_RS23495 (AXA59_23735) 63630..64853 - 1224 WP_235027232 hypothetical protein -

Host bacterium

ID   18459 GenBank   NZ_CP031566
Plasmid name   pIncLM-1301491 Incompatibility group   IncL/M
Plasmid size   64859 bp Coordinate of oriT [Strand]   6299..6403 [-]
Host baterium   Enterobacter hormaechei strain 2013_1a

Cargo genes

Drug resistance gene   blaSHV-30, ant(2'')-Ia
Virulence gene   -
Metal resistance gene   -
Degradation gene   -
Symbiosis gene   -
Anti-CRISPR   AcrIIA8