Detailed information of oriT
oriT
The information of the oriT region
| oriTDB ID | 117998 |
| Name | oriT_p1-S2-IND-01-C  |
| Organism | Klebsiella electrica strain S2-IND-01-C |
| Sequence Completeness | - |
| NCBI accession of oriT (coordinates [strand]) | NZ_CP112888 (59065..59114 [-], 50 nt) |
| oriT length | 50 nt |
| IRs (inverted repeats) | 7..14, 17..24 (GCAAAATT..AATTTTGC) |
| Location of nic site | 33..34 |
| Conserved sequence flanking the nic site |
TGTGTGGTGA |
| Note | Predicted by oriTfinder 2.0 |
oriT sequence
Download Length: 50 nt
>oriT_p1-S2-IND-01-C
AAATCTGCAAAATTTTAATTTTGCGTAGTGTGTGGTGATTTTGTGGTGAG
AAATCTGCAAAATTTTAATTTTGCGTAGTGTGTGGTGATTTTGTGGTGAG
Visualization of oriT structure
oriT secondary structure
Predicted by RNAfold.
Download structure file
Relaxase
| ID | 11416 | GenBank | WP_075606914 |
| Name | traI_OR613_RS26825_p1-S2-IND-01-C |
UniProt ID | A0A6M3HIK1 |
| Length | 1752 a.a. | PDB ID | |
| Note | Predicted by oriTfinder 2.0 | ||
Relaxase protein sequence
Download Length: 1752 a.a. Molecular weight: 190386.65 Da Isoelectric Point: 5.5084
>WP_075606914.1 MULTISPECIES: conjugative transfer relaxase/helicase TraI [Enterobacteriaceae]
MLSFSQVKSAGSAGNYYTEKDNYYVIGSMEERWQGKGAEALGLEGKVDKQVFTELLQGKLPDGSDLTRMQ
DGVNKHRPGYDLTFSAPKSVSMLAMLGGDKRLIDAHNRAVTVALNQVESLASTRVQKDGVSETVLTNNLI
IARFNHDTSRAQEPQIHTHSVVINATQNGDKWQTLGSDTVGKTGFSENILANRIAFGKIYQSTLRADVES
MGYKTVDAGKHGMWEMEGVPVESFSTRSQELREAAGPDASLKSRDVAALDTRKSKEAIDPAEKMVEWMNT
LKETGFDIRGYREAADTRAKELASAPAAPVKTDVPDISDVVTKAIAGLSDRKVQFTYADLLARTVSQLEA
KPGVFEQARTGIDAAIEREQLIPLDREKGLFTSNIHVLDELSVKALSQEIQRQNHVSVTPDTAVVRQSPF
SDAVSVLAQDRPTMGIVSGQGGAAGQRERVAELTLMAREQGRDVHIIAADNRSREFLSADARLAGETVTG
KSALQDGTAFIPGGTLIVDQAEKMSLKETLSMLDGAMRHNVQVLLSDSGKRSGTGSALTVLKESGVNTYR
WQGGKQATADIISEPEKGARYSRLAQEFAASVREGQESVAQISGSREQGILNGLIRETLKSEGVLGEKDM
TVTALTPVWLDSKSRGVRDYYREGMVMERWDPETRQHDRFVIDRVTASSNMLTLRDKEGERLDMKVSAVD
SQWTLFRADKLTVAEGERLAVLGKIPDTRLKGGESVTVLKAEEGQLTVQRPGQKTTQSLSVGSSPFDGIK
VGHGWVESPGRSVSETATVFASVTQRELDNATLNQLAQSGSHIRLYSAQGTARTTEKLARHTAFSVVSEQ
LKARSGETDLDAAIASQKAGLHTPAEQVIHLSIPLLESEELTFTRPQLLATALETGGGKVPMTEIDATIQ
SQIQAGSLLNVPVAPGHGNDLLISRQTWDAEKSILTKVLEGKEAVAPLMDNVPGSLMTDLTSGQRASTRM
ILETTDRFTVVQGYAGVGKTTQFRAVTGAISLLPEATRPRVIGLGPTHRAVGEMQSAGVDAQTTASFLHD
TQLLQRNGQTPDFSNTLFLLDESSMVGLADTAKALSLIAAGGGRAVLSGDTDQLQSIAPGQPFRLMQQRS
AADVAIMKEIVRQVPELRPAVYSLIDRDVNRALATIEQVTPEQVPRKDGAWVPENSVVEFTPAQEEAIQK
ALNKGESVPAGQPTTLYEALVKDYADRTPEAQSQTLIITHLNDDRRVLNSLIHDARRDNGEVGREEVTLP
VLVTSNIRDGELRRLSTWTAHKGAVALVDNVYHRIGSVDKENQLITLMDGEGKERLISPREALAEGVTLY
REQEITVSQGDKMRFSKSDTERGYVANSVWEVKSVSGDSVTLSDGKSTRTLNPKADQAQQHIDLAYAITA
HGAQGASEPFAIALEGVAGGRERMASFESAYVGLSRMKQHVQVYTDNREGWIKAINNSPEKATAHDILEP
RNDRAVKTAGQLFSRARALDEAAAGRAALQQSGLARGQSPGKFISPGKKYPQPHVALPAFDKNGKDAGIW
LSPLTDNDGRLQVIGGEGRIMGNEDARFVALQNSRNGESLLAGNMGEGVRIARDNPDSGVVVRLAGDDRP
WNPEAITGGRIWADPLPNPPINDTGTDIPLPPEVLAQRAAEEAQRREMERQTEQATREVSGEEKKAGEPG
ERIKEVIGDVIRGLERDRPGEEKTVLPDDPQTRRQEAAVQQVVSESLQRDRLQQMERDMVRDLNREKTLG
GD
MLSFSQVKSAGSAGNYYTEKDNYYVIGSMEERWQGKGAEALGLEGKVDKQVFTELLQGKLPDGSDLTRMQ
DGVNKHRPGYDLTFSAPKSVSMLAMLGGDKRLIDAHNRAVTVALNQVESLASTRVQKDGVSETVLTNNLI
IARFNHDTSRAQEPQIHTHSVVINATQNGDKWQTLGSDTVGKTGFSENILANRIAFGKIYQSTLRADVES
MGYKTVDAGKHGMWEMEGVPVESFSTRSQELREAAGPDASLKSRDVAALDTRKSKEAIDPAEKMVEWMNT
LKETGFDIRGYREAADTRAKELASAPAAPVKTDVPDISDVVTKAIAGLSDRKVQFTYADLLARTVSQLEA
KPGVFEQARTGIDAAIEREQLIPLDREKGLFTSNIHVLDELSVKALSQEIQRQNHVSVTPDTAVVRQSPF
SDAVSVLAQDRPTMGIVSGQGGAAGQRERVAELTLMAREQGRDVHIIAADNRSREFLSADARLAGETVTG
KSALQDGTAFIPGGTLIVDQAEKMSLKETLSMLDGAMRHNVQVLLSDSGKRSGTGSALTVLKESGVNTYR
WQGGKQATADIISEPEKGARYSRLAQEFAASVREGQESVAQISGSREQGILNGLIRETLKSEGVLGEKDM
TVTALTPVWLDSKSRGVRDYYREGMVMERWDPETRQHDRFVIDRVTASSNMLTLRDKEGERLDMKVSAVD
SQWTLFRADKLTVAEGERLAVLGKIPDTRLKGGESVTVLKAEEGQLTVQRPGQKTTQSLSVGSSPFDGIK
VGHGWVESPGRSVSETATVFASVTQRELDNATLNQLAQSGSHIRLYSAQGTARTTEKLARHTAFSVVSEQ
LKARSGETDLDAAIASQKAGLHTPAEQVIHLSIPLLESEELTFTRPQLLATALETGGGKVPMTEIDATIQ
SQIQAGSLLNVPVAPGHGNDLLISRQTWDAEKSILTKVLEGKEAVAPLMDNVPGSLMTDLTSGQRASTRM
ILETTDRFTVVQGYAGVGKTTQFRAVTGAISLLPEATRPRVIGLGPTHRAVGEMQSAGVDAQTTASFLHD
TQLLQRNGQTPDFSNTLFLLDESSMVGLADTAKALSLIAAGGGRAVLSGDTDQLQSIAPGQPFRLMQQRS
AADVAIMKEIVRQVPELRPAVYSLIDRDVNRALATIEQVTPEQVPRKDGAWVPENSVVEFTPAQEEAIQK
ALNKGESVPAGQPTTLYEALVKDYADRTPEAQSQTLIITHLNDDRRVLNSLIHDARRDNGEVGREEVTLP
VLVTSNIRDGELRRLSTWTAHKGAVALVDNVYHRIGSVDKENQLITLMDGEGKERLISPREALAEGVTLY
REQEITVSQGDKMRFSKSDTERGYVANSVWEVKSVSGDSVTLSDGKSTRTLNPKADQAQQHIDLAYAITA
HGAQGASEPFAIALEGVAGGRERMASFESAYVGLSRMKQHVQVYTDNREGWIKAINNSPEKATAHDILEP
RNDRAVKTAGQLFSRARALDEAAAGRAALQQSGLARGQSPGKFISPGKKYPQPHVALPAFDKNGKDAGIW
LSPLTDNDGRLQVIGGEGRIMGNEDARFVALQNSRNGESLLAGNMGEGVRIARDNPDSGVVVRLAGDDRP
WNPEAITGGRIWADPLPNPPINDTGTDIPLPPEVLAQRAAEEAQRREMERQTEQATREVSGEEKKAGEPG
ERIKEVIGDVIRGLERDRPGEEKTVLPDDPQTRRQEAAVQQVVSESLQRDRLQQMERDMVRDLNREKTLG
GD
Protein domains
Predicted by InterproScan.
Protein structure
No available structure.
Auxiliary protein
| ID | 6561 | GenBank | WP_053390193 |
| Name | WP_053390193_p1-S2-IND-01-C |
UniProt ID | A0A8G2A327 |
| Length | 130 a.a. | PDB ID | _ |
| Note | Predicted by oriTfinder 2.0 | ||
Auxiliary protein sequence
Download Length: 130 a.a. Molecular weight: 14883.89 Da Isoelectric Point: 4.3779
>WP_053390193.1 MULTISPECIES: conjugal transfer relaxosome DNA-binding protein TraM [Enterobacteriaceae]
MAKVQAYVSDEVADKINAIVEKRRVEGAKDKDISFSSISTMLLELGLRVYEAQMERKESGFNQMAFNKAL
LESVIKTQFTVNKVLGIECLSPHVSGNPRWEWPGLIENIRDDVQEVMLRFFPDEESEDEE
MAKVQAYVSDEVADKINAIVEKRRVEGAKDKDISFSSISTMLLELGLRVYEAQMERKESGFNQMAFNKAL
LESVIKTQFTVNKVLGIECLSPHVSGNPRWEWPGLIENIRDDVQEVMLRFFPDEESEDEE
Protein domains
Predicted by InterproScan.
Protein structure
No available structure.
T4CP
| ID | 13343 | GenBank | WP_075606915 |
| Name | traD_OR613_RS26820_p1-S2-IND-01-C |
UniProt ID | _ |
| Length | 772 a.a. | PDB ID | _ |
| Note | Predicted by oriTfinder 2.0 | ||
T4CP protein sequence
Download Length: 772 a.a. Molecular weight: 86712.47 Da Isoelectric Point: 5.0825
>WP_075606915.1 MULTISPECIES: type IV conjugative transfer system coupling protein TraD [Enterobacteriaceae]
MSFNAKDMTQGGQIANMRFRMFGQIANIIFYVLFIIFWVLCGLILMYRLSWQTFVNGCVYWWCTTLAPMR
DIIRSQPVYTINYYGKSLEYNSEQILADKYTIWCGEQLWTSFVFAAIVSLTVCIVTFFVASWVLGRQGKQ
QSEDENTGGRQLSDKPKEVARQMKRDGVASDIKIGDLPILKHSEIQNFCLHGTVGSGKSEVIRRLLNYVR
ARGDMAIIYDRSCEFVKSYYDPSLDKILNPLDSRCAAWDIWRECLTLPDFDNVSNTLIPMGTKEDPFWQG
SGRTIFSEGAYLMREDKDRSYEKLVDTMLSIKIDKLRAYLKNTPAANLVEEKIEKTAISIRAVLTNYVKA
IRYLQGIEKNGEPFTIRDWMRGVREDQPNGWLFISSNADTHASLKPVISMWLSIAIRGLLGMGENRNRRV
WIFADELPTLHKLPDLVEILPEARKFGGCYVFGIQSYAQLEDIYGVKPAATLFDVMNTRAFFRSPSKEIA
EFAAGEIGEKEILKASEQYSYGADPVRDGVSTGKEKERETLVSYSDIQTLPDLSCYVTLPGPYPAVKLAL
KYKSRPKIAEGFILRNLDTRTDARLDTLLEAREAESSHARTLFTPDAPAPELAEKDEKVGNPPEQSQPAE
SSGTPATVKVPSTAKTPEADESGQSPEVSNPAALLTKVVTVPLTRPRPAAATGTAAVASATDVRATPAGG
TEQALETQPAEQGQDMLPPGVNEYGEIDDMHAWDEWQSGEQTQRDMQRREEVNINHSHRRDEQDDIEIGG
NF
MSFNAKDMTQGGQIANMRFRMFGQIANIIFYVLFIIFWVLCGLILMYRLSWQTFVNGCVYWWCTTLAPMR
DIIRSQPVYTINYYGKSLEYNSEQILADKYTIWCGEQLWTSFVFAAIVSLTVCIVTFFVASWVLGRQGKQ
QSEDENTGGRQLSDKPKEVARQMKRDGVASDIKIGDLPILKHSEIQNFCLHGTVGSGKSEVIRRLLNYVR
ARGDMAIIYDRSCEFVKSYYDPSLDKILNPLDSRCAAWDIWRECLTLPDFDNVSNTLIPMGTKEDPFWQG
SGRTIFSEGAYLMREDKDRSYEKLVDTMLSIKIDKLRAYLKNTPAANLVEEKIEKTAISIRAVLTNYVKA
IRYLQGIEKNGEPFTIRDWMRGVREDQPNGWLFISSNADTHASLKPVISMWLSIAIRGLLGMGENRNRRV
WIFADELPTLHKLPDLVEILPEARKFGGCYVFGIQSYAQLEDIYGVKPAATLFDVMNTRAFFRSPSKEIA
EFAAGEIGEKEILKASEQYSYGADPVRDGVSTGKEKERETLVSYSDIQTLPDLSCYVTLPGPYPAVKLAL
KYKSRPKIAEGFILRNLDTRTDARLDTLLEAREAESSHARTLFTPDAPAPELAEKDEKVGNPPEQSQPAE
SSGTPATVKVPSTAKTPEADESGQSPEVSNPAALLTKVVTVPLTRPRPAAATGTAAVASATDVRATPAGG
TEQALETQPAEQGQDMLPPGVNEYGEIDDMHAWDEWQSGEQTQRDMQRREEVNINHSHRRDEQDDIEIGG
NF
Protein domains
Predicted by InterproScan.
Protein structure
No available structure.
T4SS
T4SS were predicted by using oriTfinder2.
Region 1: 58498..87782
| Locus tag | Coordinates | Strand | Size (bp) | Protein ID | Product | Description |
|---|---|---|---|---|---|---|
| OR613_RS26615 (OR613_26610) | 53688..54563 | + | 876 | WP_060415463 | DUF4942 domain-containing protein | - |
| OR613_RS26620 (OR613_26615) | 55343..55681 | + | 339 | WP_020804855 | hypothetical protein | - |
| OR613_RS26625 (OR613_26620) | 55738..56085 | + | 348 | WP_064182675 | hypothetical protein | - |
| OR613_RS26630 (OR613_26625) | 56174..56404 | + | 231 | WP_060415465 | hypothetical protein | - |
| OR613_RS26635 (OR613_26630) | 56451..57284 | + | 834 | WP_060415466 | N-6 DNA methylase | - |
| OR613_RS26640 (OR613_26635) | 57570..57938 | - | 369 | WP_080848162 | hypothetical protein | - |
| OR613_RS26645 (OR613_26640) | 57933..58475 | + | 543 | WP_060415467 | antirestriction protein | - |
| OR613_RS26650 (OR613_26645) | 58498..58920 | - | 423 | WP_073972322 | transglycosylase SLT domain-containing protein | virB1 |
| OR613_RS26655 (OR613_26650) | 59426..59818 | + | 393 | WP_053390193 | conjugal transfer relaxosome DNA-binding protein TraM | - |
| OR613_RS26660 (OR613_26655) | 60056..60757 | + | 702 | WP_094898581 | hypothetical protein | - |
| OR613_RS26665 (OR613_26660) | 60918..61079 | + | 162 | WP_172686697 | TraY domain-containing protein | - |
| OR613_RS26670 (OR613_26665) | 61144..61512 | + | 369 | WP_064386081 | type IV conjugative transfer system pilin TraA | - |
| OR613_RS26675 (OR613_26670) | 61526..61831 | + | 306 | WP_064343526 | type IV conjugative transfer system protein TraL | traL |
| OR613_RS26680 (OR613_26675) | 61847..62413 | + | 567 | WP_064359954 | type IV conjugative transfer system protein TraE | traE |
| OR613_RS26685 (OR613_26680) | 62400..63134 | + | 735 | WP_064343528 | type-F conjugative transfer system secretin TraK | traK |
| OR613_RS26690 (OR613_26685) | 63134..64555 | + | 1422 | WP_053390199 | F-type conjugal transfer pilus assembly protein TraB | traB |
| OR613_RS26695 (OR613_26690) | 64548..64982 | + | 435 | WP_020322358 | conjugal transfer protein TraP | - |
| OR613_RS26700 (OR613_26695) | 65016..65600 | + | 585 | WP_064343530 | type IV conjugative transfer system lipoprotein TraV | traV |
| OR613_RS26705 (OR613_26700) | 66213..66401 | + | 189 | WP_053390286 | hypothetical protein | - |
| OR613_RS26710 (OR613_26705) | 66485..66886 | + | 402 | WP_064343532 | hypothetical protein | - |
| OR613_RS26715 (OR613_26710) | 66883..67182 | + | 300 | WP_053390308 | hypothetical protein | - |
| OR613_RS26720 (OR613_26715) | 67270..67653 | + | 384 | WP_042946290 | hypothetical protein | - |
| OR613_RS26725 (OR613_26720) | 67746..70385 | + | 2640 | WP_064343533 | type IV secretion system protein TraC | virb4 |
| OR613_RS26730 (OR613_26725) | 70385..70777 | + | 393 | WP_064343534 | type-F conjugative transfer system protein TrbI | - |
| OR613_RS26735 (OR613_26730) | 70774..71400 | + | 627 | WP_064343535 | type-F conjugative transfer system protein TraW | traW |
| OR613_RS26740 (OR613_26735) | 71444..72403 | + | 960 | WP_229295972 | conjugal transfer pilus assembly protein TraU | traU |
| OR613_RS26745 (OR613_26740) | 72416..73063 | + | 648 | WP_064343536 | type-F conjugative transfer system pilin assembly protein TrbC | trbC |
| OR613_RS26750 (OR613_26745) | 73060..73443 | + | 384 | WP_053390297 | hypothetical protein | - |
| OR613_RS26755 (OR613_26750) | 73440..75395 | + | 1956 | WP_064343537 | type-F conjugative transfer system mating-pair stabilization protein TraN | traN |
| OR613_RS26760 (OR613_26755) | 75427..75687 | + | 261 | WP_053390216 | hypothetical protein | - |
| OR613_RS26765 (OR613_26760) | 75680..76291 | + | 612 | WP_053390217 | hypothetical protein | - |
| OR613_RS26770 (OR613_26765) | 76281..76523 | + | 243 | WP_053390218 | conjugal transfer protein TrbE | - |
| OR613_RS26775 (OR613_26770) | 76569..76895 | + | 327 | WP_053390219 | hypothetical protein | - |
| OR613_RS26780 (OR613_26775) | 76916..77668 | + | 753 | WP_053390220 | type-F conjugative transfer system pilin assembly protein TraF | traF |
| OR613_RS26785 (OR613_26780) | 77679..77915 | + | 237 | WP_053390221 | type-F conjugative transfer system pilin chaperone TraQ | - |
| OR613_RS26790 (OR613_26785) | 77890..78450 | + | 561 | WP_039698500 | type-F conjugative transfer system pilin assembly thiol-disulfide isomerase TrbB | traF |
| OR613_RS26795 (OR613_26790) | 78437..79807 | + | 1371 | WP_075606918 | conjugal transfer pilus assembly protein TraH | traH |
| OR613_RS26800 (OR613_26795) | 79807..82686 | + | 2880 | WP_064386194 | conjugal transfer mating-pair stabilization protein TraG | traG |
| OR613_RS26805 (OR613_26800) | 82703..83371 | + | 669 | WP_128317877 | hypothetical protein | - |
| OR613_RS26810 (OR613_26805) | 83730..84461 | + | 732 | WP_064386198 | conjugal transfer complement resistance protein TraT | - |
| OR613_RS26815 (OR613_26810) | 84653..85345 | + | 693 | WP_202395549 | hypothetical protein | - |
| OR613_RS26820 (OR613_26815) | 85464..87782 | + | 2319 | WP_075606915 | type IV conjugative transfer system coupling protein TraD | virb4 |
Host bacterium
| ID | 18431 | GenBank | NZ_CP112888 |
| Plasmid name | p1-S2-IND-01-C | Incompatibility group | IncFII |
| Plasmid size | 98913 bp | Coordinate of oriT [Strand] | 59065..59114 [-] |
| Host baterium | Klebsiella electrica strain S2-IND-01-C |
Cargo genes
| Drug resistance gene | - |
| Virulence gene | - |
| Metal resistance gene | - |
| Degradation gene | - |
| Symbiosis gene | - |
| Anti-CRISPR | - |