Detailed information of oriT
oriT
The information of the oriT region
| oriTDB ID | 117996 |
| Name | oriT_unnamed2  |
| Organism | Klebsiella electrica strain DSM 102253 |
| Sequence Completeness | - |
| NCBI accession of oriT (coordinates [strand]) | NZ_CP041249 (30363..30412 [-], 50 nt) |
| oriT length | 50 nt |
| IRs (inverted repeats) | 7..14, 17..24 (GCAAAATT..AATTTTGC) |
| Location of nic site | 33..34 |
| Conserved sequence flanking the nic site |
TGTGTGGTGA |
| Note | Predicted by oriTfinder 2.0 |
oriT sequence
Download Length: 50 nt
>oriT_unnamed2
AAATCTGCAAAATTTCAATTTTGCGTAGTGTGTGGTGATTTTGTGGTGAG
AAATCTGCAAAATTTCAATTTTGCGTAGTGTGTGGTGATTTTGTGGTGAG
Visualization of oriT structure
oriT secondary structure
Predicted by RNAfold.
Download structure file
T4CP
| ID | 13342 | GenBank | WP_142255972 |
| Name | traD_Electrica_RS27390_unnamed2 |
UniProt ID | _ |
| Length | 772 a.a. | PDB ID | _ |
| Note | Predicted by oriTfinder 2.0 | ||
T4CP protein sequence
Download Length: 772 a.a. Molecular weight: 86488.25 Da Isoelectric Point: 5.0245
>WP_142255972.1 type IV conjugative transfer system coupling protein TraD [Klebsiella electrica]
MSFNAKDMTQGGQIANMRFRMFGQIANIIFYVLFIIFWVLCGLILMYRLSWQTFVNGCVYWWCTTLAPMR
DIIRSQPVYTINYYGKSLEYNSEQILADKYTIWCGEQLWTSFVFAAIVSLTVCIVTFFVASWVLGRQGKQ
QSEDENTGGRQLSDKPKEVARQMKRDGVASDIKIGDLPILKNSEIQNFCLHGTVGSGKSEVIRRLLNYVR
ARGDMAIIYDRSCEFVKSYYDPSLDKILNPLDSRCAAWDIWRECLTLPDFDNVSNTLIPMGTKEDPFWQG
SGRTIFSEGAYLMREDKDRSYEKLVDTMLSIKIDKLRAYLKNTPAANLVEEKIEKTAISIRAVLTNYVKA
IRYLQGIEKNGEPFTIRDWMRGVREDQPNGWLFISSNADTHASLKPVISMWLSIAIRGLLGMGENRNRRV
WIFADELPTLHKLPDLVEILPEARKFGGCYVFGIQSYAQLEDIYGVKPAATLFDVMNTRAFFRSPSKEIA
EFAAGEIGEKEILKASEQYSYGADPVRDGVSTGKEKERETLVSYSDVQTLPDLSCYVTLPGPYPAVKLAL
KYKPRPKIAEGFIQRNLDTRVDARLNALLEAREAEGSLARTLFTPDAPAPELAEKDEKVGNPPEQSQPAE
SSGTSATVKVPSTTKTPAADESGQSPEVSNPAALLTKVVTVPLTRPRPAAATGTAAAASATDVPATPAGG
TEQALETQPAEQGQDMLPPGVNEYGEIDDMHAWDEWQSGEQTQRDMQRREEVNINHSHRRDEQDDIEIGG
NF
MSFNAKDMTQGGQIANMRFRMFGQIANIIFYVLFIIFWVLCGLILMYRLSWQTFVNGCVYWWCTTLAPMR
DIIRSQPVYTINYYGKSLEYNSEQILADKYTIWCGEQLWTSFVFAAIVSLTVCIVTFFVASWVLGRQGKQ
QSEDENTGGRQLSDKPKEVARQMKRDGVASDIKIGDLPILKNSEIQNFCLHGTVGSGKSEVIRRLLNYVR
ARGDMAIIYDRSCEFVKSYYDPSLDKILNPLDSRCAAWDIWRECLTLPDFDNVSNTLIPMGTKEDPFWQG
SGRTIFSEGAYLMREDKDRSYEKLVDTMLSIKIDKLRAYLKNTPAANLVEEKIEKTAISIRAVLTNYVKA
IRYLQGIEKNGEPFTIRDWMRGVREDQPNGWLFISSNADTHASLKPVISMWLSIAIRGLLGMGENRNRRV
WIFADELPTLHKLPDLVEILPEARKFGGCYVFGIQSYAQLEDIYGVKPAATLFDVMNTRAFFRSPSKEIA
EFAAGEIGEKEILKASEQYSYGADPVRDGVSTGKEKERETLVSYSDVQTLPDLSCYVTLPGPYPAVKLAL
KYKPRPKIAEGFIQRNLDTRVDARLNALLEAREAEGSLARTLFTPDAPAPELAEKDEKVGNPPEQSQPAE
SSGTSATVKVPSTTKTPAADESGQSPEVSNPAALLTKVVTVPLTRPRPAAATGTAAAASATDVPATPAGG
TEQALETQPAEQGQDMLPPGVNEYGEIDDMHAWDEWQSGEQTQRDMQRREEVNINHSHRRDEQDDIEIGG
NF
Protein domains
Predicted by InterproScan.
Protein structure
No available structure.
T4SS
T4SS were predicted by using oriTfinder2.
Region 1: 32792..57642
| Locus tag | Coordinates | Strand | Size (bp) | Protein ID | Product | Description |
|---|---|---|---|---|---|---|
| Electrica_RS27210 | 28757..29236 | - | 480 | WP_142255947 | hypothetical protein | - |
| Electrica_RS27215 (electrica_05461) | 29231..29773 | + | 543 | WP_064343524 | antirestriction protein | - |
| Electrica_RS27220 (electrica_05462) | 29796..30290 | - | 495 | WP_228267449 | transglycosylase SLT domain-containing protein | - |
| Electrica_RS27225 (electrica_05463) | 30720..31109 | + | 390 | WP_142255948 | conjugal transfer relaxosome DNA-binding protein TraM | - |
| Electrica_RS27230 (electrica_05464) | 31350..32054 | + | 705 | WP_142255949 | hypothetical protein | - |
| Electrica_RS27235 (electrica_05465) | 32139..32339 | + | 201 | WP_142255950 | TraY domain-containing protein | - |
| Electrica_RS27240 (electrica_05466) | 32410..32778 | + | 369 | WP_142255951 | type IV conjugative transfer system pilin TraA | - |
| Electrica_RS27245 (electrica_05467) | 32792..33097 | + | 306 | WP_032744112 | type IV conjugative transfer system protein TraL | traL |
| Electrica_RS27250 (electrica_05468) | 33116..33682 | + | 567 | WP_032744110 | type IV conjugative transfer system protein TraE | traE |
| Electrica_RS27255 (electrica_05469) | 33669..34403 | + | 735 | WP_142255952 | type-F conjugative transfer system secretin TraK | traK |
| Electrica_RS27260 (electrica_05470) | 34403..35827 | + | 1425 | WP_142255953 | F-type conjugal transfer pilus assembly protein TraB | traB |
| Electrica_RS27265 (electrica_05471) | 35890..36459 | + | 570 | WP_095104641 | type IV conjugative transfer system lipoprotein TraV | traV |
| Electrica_RS29080 (electrica_05472) | 36483..36851 | + | 369 | WP_224253886 | hypothetical protein | - |
| Electrica_RS27275 (electrica_05473) | 36858..37100 | + | 243 | WP_142255954 | hypothetical protein | - |
| Electrica_RS27280 (electrica_05474) | 37097..37501 | + | 405 | WP_142255955 | hypothetical protein | - |
| Electrica_RS27285 (electrica_05475) | 37498..37788 | + | 291 | WP_142255956 | hypothetical protein | - |
| Electrica_RS27290 (electrica_05476) | 37785..38183 | + | 399 | WP_228267450 | hypothetical protein | - |
| Electrica_RS27295 (electrica_05477) | 38268..40907 | + | 2640 | WP_142255957 | type IV secretion system protein TraC | virb4 |
| Electrica_RS27300 (electrica_05478) | 40907..41296 | + | 390 | WP_032744089 | type-F conjugative transfer system protein TrbI | - |
| Electrica_RS27305 (electrica_05479) | 41293..41922 | + | 630 | WP_142255958 | type-F conjugative transfer system protein TraW | traW |
| Electrica_RS27310 (electrica_05480) | 41945..42922 | + | 978 | WP_208764251 | conjugal transfer pilus assembly protein TraU | traU |
| Electrica_RS27315 (electrica_05482) | 43109..43345 | - | 237 | WP_142255960 | hypothetical protein | - |
| Electrica_RS27320 (electrica_05483) | 43582..44229 | + | 648 | WP_142255961 | type-F conjugative transfer system pilin assembly protein TrbC | trbC |
| Electrica_RS27330 (electrica_05485) | 44606..46561 | + | 1956 | WP_142255963 | type-F conjugative transfer system mating-pair stabilization protein TraN | traN |
| Electrica_RS27335 (electrica_05486) | 46577..46957 | + | 381 | WP_142255964 | hypothetical protein | - |
| Electrica_RS27340 (electrica_05487) | 46947..47174 | + | 228 | WP_095104619 | conjugal transfer protein TrbE | - |
| Electrica_RS27345 (electrica_05488) | 47184..47510 | + | 327 | WP_142255965 | hypothetical protein | - |
| Electrica_RS27350 (electrica_05489) | 47533..48285 | + | 753 | WP_032744065 | type-F conjugative transfer system pilin assembly protein TraF | traF |
| Electrica_RS27355 (electrica_05490) | 48296..48532 | + | 237 | WP_142255966 | type-F conjugative transfer system pilin chaperone TraQ | - |
| Electrica_RS27360 (electrica_05491) | 48507..49073 | + | 567 | WP_142255967 | type-F conjugative transfer system pilin assembly thiol-disulfide isomerase TrbB | traF |
| Electrica_RS27365 (electrica_05492) | 49066..49452 | + | 387 | WP_142255968 | hypothetical protein | - |
| Electrica_RS27370 (electrica_05493) | 49427..50809 | + | 1383 | WP_167686289 | conjugal transfer pilus assembly protein TraH | traH |
| Electrica_RS27375 (electrica_05494) | 50809..53652 | + | 2844 | WP_142255970 | conjugal transfer mating-pair stabilization protein TraG | traG |
| Electrica_RS27380 (electrica_05495) | 53636..54211 | + | 576 | WP_228267447 | conjugal transfer entry exclusion protein TraS | - |
| Electrica_RS27385 (electrica_05496) | 54398..55129 | + | 732 | WP_142255971 | conjugal transfer complement resistance protein TraT | - |
| Electrica_RS27390 (electrica_05497) | 55324..57642 | + | 2319 | WP_142255972 | type IV conjugative transfer system coupling protein TraD | virb4 |
| Electrica_RS27395 (electrica_05498) | 57642..58145 | + | 504 | Protein_74 | MobF family relaxase | - |
| Electrica_RS27400 (electrica_05499) | 58188..58793 | - | 606 | WP_000509966 | recombinase family protein | - |
| Electrica_RS27405 (electrica_05500) | 58888..61785 | + | 2898 | WP_023307208 | Tn3-like element Tn5403 family transposase | - |
Host bacterium
| ID | 18429 | GenBank | NZ_CP041249 |
| Plasmid name | unnamed2 | Incompatibility group | IncFII |
| Plasmid size | 92370 bp | Coordinate of oriT [Strand] | 30363..30412 [-] |
| Host baterium | Klebsiella electrica strain DSM 102253 |
Cargo genes
| Drug resistance gene | - |
| Virulence gene | - |
| Metal resistance gene | arsR, arsD |
| Degradation gene | - |
| Symbiosis gene | - |
| Anti-CRISPR | - |