Detailed information of oriT

oriT

The information of the oriT region


oriTDB ID   117933
Name   oriT_SWMUF35|pC in_silico
Organism   Klebsiella quasipneumoniae strain SWMUF35
Sequence Completeness      -
NCBI accession of oriT (coordinates [strand])   NZ_CP068447 (1708..1789 [+], 82 nt)
oriT length   82 nt
IRs (inverted repeats)      27..32, 35..40  (GCCACC..GGTGGC)
Location of nic site      _
Conserved sequence flanking the
  nic site    		  _
Note   Predicted by oriTfinder 2.0

  oriT sequence  


Download         Length: 82 nt

>oriT_SWMUF35|pC
CGGGACAGGATATGTTTTGTATTACCGCCACCACGGTGGCAGCAATCCAAAACTCCTGGTCAGGGCGAAGCCCCGACACCCC

Visualization of oriT structure

  oriT secondary structure

Predicted by RNAfold.

Download structure file


Relaxase

ID   11386 GenBank   WP_032440569
Name   traI_IT777_RS27055_SWMUF35|pC insolico UniProt ID   A0A7G3L4G1
Length   642 a.a. PDB ID   
Note   Predicted by oriTfinder 2.0

  Relaxase protein sequence


Download         Length: 642 a.a.        Molecular weight: 74384.24 Da        Isoelectric Point: 8.3203

>WP_032440569.1 MULTISPECIES: TraI/MobA(P) family conjugative relaxase [Enterobacterales]
MNAIIPPKRRDRKSSFVQLVAYVSVRDDVPLKDELKEDERFQRPSRSRQAIFDRLVDYMNRSEGTIIEQI
SDMTPEGDIRCSVDGVICQHNLMSVETAAMEMNSIAMQNTHVKDAVYHYILSWQEDENPSDDQVFDSVKH
SLKRLGMEEHQYVAAIHRDTDNLHVHIAANRVHPVSYRAANVWNDADKLQRTCRELELKHGFKVDNGSWV
RDADNNIVRARKGYRNAPRSARQLEHFSDKESLHHFAVQHVRKPVNAMFREKNASWEKLHQVMNDAGLML
ETSGKGLIVRDVCGDEKLAVKSSRVHPGMTLSRLEPALGTFVPSPRSAEMVKQSVQTSYSDKLHVRDRGA
RAERRQARADARIDLRERYEKYRNAWKKPDLHAAERFRAVSAAFKEQKTHIRDNEKDAHMRKLMYHIVEF
EREKSMAALRIALKAERQKLYDEGMMRPLTYRAWTEQQALHGDQAALSQLRGWSYRQNRKNRTPQASDNI
IYCAPADDTSLLKADGYEARLHRDGAVVYSRQGQDAIIDRGDRVEVINPYDDGDLNTRIAVDMVAWKSGE
RVEFSRDGSFAYAAGDAVAAHNLHHRDSIIVPSDREQYDYTREKFNQLKGRYTDSEREALRQQYDDRYYD
DVRHNDDSSYKP

  Protein domains


Predicted by InterproScan.

Relaxase/Mobilisation nuclease domain (Relaxase)

(52-307)


  Protein structure


Source ID Structure
AlphaFold DB A0A7G3L4G1


T4CP

ID   13300 GenBank   WP_196611376
Name   trbC_IT777_RS27150_SWMUF35|pC insolico UniProt ID   _
Length   710 a.a. PDB ID   _
Note   Predicted by oriTfinder 2.0

  T4CP protein sequence


Download         Length: 710 a.a.        Molecular weight: 80321.08 Da        Isoelectric Point: 5.7665

>WP_196611376.1 MULTISPECIES: F-type conjugative transfer protein TrbC [Klebsiella]
MVEEMAYRTGPLLLAIIAIFGAAIVWPVLLWAAIPVMLVWVPMFMLNEFRMPARMPMDMDRLDPSTETMR
PGKLLGFLPVTKLNRHMEKAAGVFYTGYERTLDGGREIWLRMDDLTRHLILMATTGAGKTEMLLGFVLNA
LCWAKGLIFSDGKAQNDVWFAVASLSRRFGREDDLRLLNYITGGQSRSQRLLENDKSRQQSNNTNPFALA
QETYITQLMDSMMPEAGNDRTWQEKAKGMNQVLTKALIYKSRKEQKVMSQRLIQEYLSLDKFAELYLQAE
AEEWHEEIRTGLRNYLSTSVPGFDMSLITKPSEWSQEARNQHGYLTSQYNRMLSLFSDTYGHVFSSGAGD
IDLRDVVHNDRILVILIPALELSASEALTLGRLTTSQLAMILSQDLGERMEGKAEDILVIKKFRDRFPFL
WLADEVGSYYSDTIGQLATQVRSLGYALVLAGQDLQKLKTAVGDKLWTLLGNMFTRIFGTIIDPTETAEF
AAKSAGMEYQAIQDSMEYRAGAIGGSYADTGRVTVQEKPKLPFDELQALNQGEQATVFKGAVIRNNALYI
ADDDKITDKDVRINRFVEVEWPTYDSLSHLLPAKVRRDRPRPASINRIMAIVKNDKIRHAPDSMIIQDGV
LYAIADEAMYFDEVAPTPPNTVQRATQLLSLAAAVLTETRGRYRVEYPEPEKLSVKKDELEKYQHIHQSA
VTQQADGFSY

  Protein domains


Predicted by InterproScan.

TraM recognition site of TraD and TraG (TraG-D_C)

(419-540)

  Protein structure



No available structure.




T4SS

T4SS were predicted by using oriTfinder2.

Region 1: 10898..23301

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
IT777_RS27065 (IT777_27065) 6045..6539 - 495 WP_032440571 molybdopterin-dependent oxidoreductase -
IT777_RS27070 (IT777_27070) 6532..7461 - 930 WP_032440581 glycosyltransferase family 9 protein -
IT777_RS27075 (IT777_27075) 7521..8363 - 843 WP_032440572 helix-turn-helix domain-containing protein -
IT777_RS27080 (IT777_27080) 8973..9200 + 228 WP_032440573 hypothetical protein -
IT777_RS27085 (IT777_27085) 9932..10411 + 480 WP_032440574 lytic transglycosylase domain-containing protein -
IT777_RS27090 (IT777_27090) 10437..10901 + 465 WP_225546581 DotD/TraH family lipoprotein -
IT777_RS27095 (IT777_27095) 10898..11716 + 819 WP_032440576 type IV secretory system conjugative DNA transfer family protein traI
IT777_RS27100 (IT777_27100) 11694..12881 + 1188 WP_077255878 plasmid transfer ATPase TraJ virB11
IT777_RS27105 (IT777_27105) 12878..13144 + 267 WP_032440577 IcmT/TraK family protein traK
IT777_RS27110 (IT777_27110) 13162..16575 + 3414 WP_052145516 LPD7 domain-containing protein -
IT777_RS27115 (IT777_27115) 16609..16962 + 354 WP_032440584 hypothetical protein traL
IT777_RS27120 (IT777_27120) 17034..17702 + 669 WP_032440585 DotI/IcmL/TraM family protein traM
IT777_RS27125 (IT777_27125) 17713..18816 + 1104 WP_077264204 DotH/IcmK family type IV secretion protein traN
IT777_RS27130 (IT777_27130) 18820..20160 + 1341 WP_032440586 conjugal transfer protein TraO traO
IT777_RS27135 (IT777_27135) 20167..20886 + 720 WP_050597333 conjugal transfer protein TraP traP
IT777_RS27140 (IT777_27140) 20925..22199 + 1275 WP_188028211 conjugal transfer protein trbA
IT777_RS27145 (IT777_27145) 22180..23301 + 1122 WP_052455213 thioredoxin fold domain-containing protein trbB
IT777_RS27150 (IT777_27150) 23363..25495 + 2133 WP_196611376 F-type conjugative transfer protein TrbC -
IT777_RS27155 (IT777_27155) 25541..26074 + 534 WP_032440594 phospholipase D family protein -
IT777_RS27160 (IT777_27160) 26086..26337 + 252 WP_113394424 hypothetical protein -
IT777_RS27165 (IT777_27165) 26843..27538 + 696 WP_254910806 RepA family replication protein -

Host bacterium

ID   18366 GenBank   NZ_CP068447
Plasmid name   SWMUF35|pC Incompatibility group   IncFII
Plasmid size   61574 bp Coordinate of oriT [Strand]   1708..1789 [+]
Host baterium   Klebsiella quasipneumoniae strain SWMUF35

Cargo genes

Drug resistance gene   blaTEM-1B, blaCTX-M-3, qnrS1
Virulence gene   -
Metal resistance gene   -
Degradation gene   -
Symbiosis gene   -
Anti-CRISPR   -